FastQCFastQC Report
Mon 6 Feb 2017
SRR2077151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269926
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35620.1089321287393048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71900.016.7700541
ACGGACC2007.2759576E-1116.5025358
GGTATCA43350.016.2421531
CGTATAC850.002978950315.5313233
GCGTATC1004.9417006E-415.4021311
GTACCGA1158.2168044E-515.3059986
CTATACT3600.014.6691464
CTTATAG9150.014.6684723
CCTATAC2701.8189894E-1214.6684713
GTATTAG3250.014.2173521
TTATAGG7650.014.0938854
CGATTCG1251.676954E-414.07548839
TATAGGC7750.013.9118165
GTACATG94250.013.8905161
TCGGACG1453.854085E-513.64975343
TACATGG95900.013.605222
GCGCACT3151.8189894E-1213.26457442
TAAGGTG6900.013.0744735
GTACTAG3203.6379788E-1213.0643081
ACATGGG97850.013.0419733
Produced by FastQC (version 0.11.5)