FastQCFastQC Report
Mon 6 Feb 2017
SRR2077150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451230
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52000.022.4708671
GGTATCA25400.021.5721931
CGTGCCG2450.018.85327510
TATCAAC64650.017.7673152
ATCAACG65000.017.6381513
TCAACGC65250.017.469044
CAACGCA66100.017.3099215
GCGTATC902.212412E-417.1152881
AACGCAG67300.017.0284066
GTACATG102350.014.5340791
GCACCGT3950.014.4786366
ACGCAGA79500.014.2492317
GAGTACG1707.578619E-714.238771
ACCGTGC5100.014.2338288
TACATGG102750.014.07032
AGAGTAC75950.013.75679311
CGCAGAG81250.013.7265858
GTCTTAC3450.013.3945731
GTATAGA4450.013.3515721
GTGTAAG6600.013.3365881
Produced by FastQC (version 0.11.5)