##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077150_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2451230
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.565724962569814	31.0	30.0	34.0	16.0	34.0
2	28.63171469017595	31.0	30.0	34.0	16.0	34.0
3	28.68164880488571	31.0	30.0	34.0	16.0	34.0
4	32.01532659113996	35.0	33.0	37.0	18.0	37.0
5	31.990571264222453	35.0	33.0	37.0	18.0	37.0
6	32.02194857275735	35.0	33.0	37.0	17.0	37.0
7	31.886919628105073	35.0	33.0	37.0	17.0	37.0
8	31.807058089204194	35.0	32.0	37.0	17.0	37.0
9	33.06914651011941	38.0	33.0	39.0	17.0	39.0
10	33.00742076426937	38.0	33.0	39.0	16.0	39.0
11	33.04181900515252	38.0	33.0	39.0	16.0	39.0
12	32.92545579158219	38.0	33.0	39.0	15.0	39.0
13	32.86498982143659	38.0	33.0	39.0	13.0	39.0
14	33.742027471922256	39.0	33.0	41.0	11.0	41.0
15	33.779759141329045	39.0	33.0	41.0	11.0	41.0
16	33.74568889904252	39.0	33.0	41.0	11.0	41.0
17	33.59615825524329	38.0	33.0	41.0	10.0	41.0
18	33.567867968326105	38.0	33.0	41.0	10.0	41.0
19	33.70348151744226	39.0	33.0	41.0	10.0	41.0
20	33.68711300041204	39.0	33.0	41.0	10.0	41.0
21	33.6389241319664	39.0	33.0	41.0	10.0	41.0
22	33.56129290193087	39.0	33.0	41.0	10.0	41.0
23	33.51836996120315	39.0	33.0	41.0	10.0	41.0
24	33.52230431252881	39.0	33.0	41.0	10.0	41.0
25	33.430071025566754	39.0	32.0	41.0	10.0	41.0
26	33.37414889667636	38.0	32.0	41.0	9.0	41.0
27	33.31401745246264	38.0	32.0	41.0	8.0	41.0
28	33.20701606948349	38.0	32.0	40.0	8.0	41.0
29	33.181870326325964	38.0	32.0	40.0	8.0	41.0
30	33.01700819588533	38.0	32.0	40.0	2.0	41.0
31	32.999798876482416	38.0	32.0	40.0	2.0	41.0
32	32.857462987969306	38.0	32.0	40.0	2.0	41.0
33	32.81293432276857	38.0	32.0	40.0	2.0	41.0
34	32.72141006759872	38.0	32.0	40.0	2.0	41.0
35	32.654341289883035	38.0	31.0	40.0	2.0	41.0
36	32.55689143817594	38.0	31.0	40.0	2.0	41.0
37	32.456680931613924	38.0	31.0	40.0	2.0	41.0
38	32.36923381322846	38.0	31.0	40.0	2.0	41.0
39	32.254115280899796	38.0	31.0	40.0	2.0	41.0
40	32.18506382510005	38.0	31.0	40.0	2.0	41.0
41	32.06421633220873	38.0	31.0	40.0	2.0	41.0
42	31.919808830668686	38.0	30.0	40.0	2.0	41.0
43	31.81403785038532	38.0	30.0	40.0	2.0	41.0
44	31.655065824096475	38.0	30.0	40.0	2.0	41.0
45	31.499328092427067	38.0	30.0	40.0	2.0	41.0
46	31.350665992175358	38.0	30.0	40.0	2.0	41.0
47	31.16799647523896	37.0	30.0	40.0	2.0	41.0
48	30.984781925808676	37.0	30.0	40.0	2.0	41.0
49	30.79904496926033	37.0	30.0	40.0	2.0	41.0
50	30.5807064208581	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.5375996025553746
1101	2	1.5049204164940164
1101	3	1.606316602606725
1101	4	1.7804976314835486
1101	5	1.9629818252219664
1101	6	1.8675031447998904
1101	7	1.831004089535348
1101	8	1.9276407212895847
1101	9	2.1386679158278596
1101	10	2.1054878908568924
1101	11	2.1279679069263366
1101	12	2.1061649435142016
1101	13	2.313266470989923
1101	14	2.6464146443317773
1101	15	2.5661439763403635
1101	16	2.5710852213347835
1101	17	2.752608726890017
1101	18	2.711794886298172
1101	19	2.5958152590912533
1101	20	2.717544853827043
1101	21	2.878609957370813
1101	22	2.6791881329438496
1101	23	2.8120509679446997
1101	24	2.6516912146518195
1101	25	2.657919061129178
1101	26	2.6270986094872057
1101	27	2.7120834993698253
1101	28	2.739510475483563
1101	29	2.771851099974647
1101	30	3.0207876260561974
1101	31	2.869776530501511
1101	32	2.8010133317440946
1101	33	2.958541516455604
1101	34	2.9849453034440145
1101	35	3.0386008873368127
1101	36	3.1104723001415167
1101	37	3.1180336307011984
1101	38	2.9995255982493205
1101	39	3.1544063111143714
1101	40	3.099207389170104
1101	41	3.1452025037194744
1101	42	3.1315007258283956
1101	43	2.9842986605560355
1101	44	3.0258899126740353
1101	45	3.0442543397465442
1101	46	3.0371263170471856
1101	47	3.03486512722359
1101	48	3.127262155209788
1101	49	3.026698920112036
1101	50	3.0141310571683064
1102	1	0.5332032361452761
1102	2	0.5775429037618132
1102	3	0.5249260379209666
1102	4	0.6067294384933888
1102	5	0.6441782286151607
1102	6	0.6125069586824239
1102	7	0.8116642379300529
1102	8	0.8078046797144154
1102	9	0.8383897720714586
1102	10	0.9540197387022431
1102	11	0.7977257446475647
1102	12	0.7427520194605464
1102	13	0.8828316883812306
1102	14	1.1101388428385803
1102	15	0.9188857341164507
1102	16	1.0089374276080463
1102	17	1.1381876280295629
1102	18	1.0781980483535136
1102	19	0.9913202710722331
1102	20	0.9111375311725851
1102	21	0.9788927240151395
1102	22	1.0307117597535154
1102	23	1.0114407467395097
1102	24	0.8517397549749646
1102	25	1.0332967895961502
1102	26	1.0077587578819092
1102	27	0.9685813535793457
1102	28	1.131341832532307
1102	29	0.9458481259167542
1102	30	1.0624737473761812
1102	31	1.0813961592836066
1102	32	1.1180910501948063
1102	33	1.120031319135336
1102	34	1.1036549013298185
1102	35	1.0628949338276499
1102	36	1.141102014524094
1102	37	1.0243962654391225
1102	38	1.1041407813542108
1102	39	1.1271089284394193
1102	40	1.1998676916610762
1102	41	1.0271601426565198
1102	42	0.9913201250070642
1102	43	0.9739618831711994
1102	44	0.893472681629806
1102	45	1.0571760819589073
1102	46	1.0784888362129088
1102	47	0.9945196433998476
1102	48	0.9976140340284836
1102	49	1.0041592595861069
1102	50	0.9968946195657402
1103	1	0.25346211813016595
1103	2	0.34855178394252206
1103	3	0.26318208012437694
1103	4	0.350492203076584
1103	5	0.42428803109338986
1103	6	0.3751716804415679
1103	7	0.33123771141219294
1103	8	0.5476476798515364
1103	9	0.6483839445656301
1103	10	0.39825754424035864
1103	11	0.5933595355212944
1103	12	0.5001780299825285
1103	13	0.6343776913978232
1103	14	0.5928482902539116
1103	15	0.5963039686167235
1103	16	0.41199145562337236
1103	17	0.6053135490641495
1103	18	0.4564871612014443
1103	19	0.6537662358401803
1103	20	0.5127609335699788
1103	21	0.2768619374642256
1103	22	0.3176680696974401
1103	23	0.43538692440085924
1103	24	0.3183209936537281
1103	25	0.4546880660183845
1103	26	0.5120418844560604
1103	27	0.3565692944310612
1103	28	0.41245214378967887
1103	29	0.47013231695790836
1103	30	0.5079639254128026
1103	31	0.4742615240529986
1103	32	0.5681952416689668
1103	33	0.639429516397314
1103	34	0.4712822945764046
1103	35	0.5217669055092244
1103	36	0.6204214953528577
1103	37	0.6006891418093971
1103	38	0.43967422366009856
1103	39	0.6668939241454623
1103	40	0.6388160884655107
1103	41	0.6638858782212651
1103	42	0.6529333698633408
1103	43	0.5097238250476934
1103	44	0.5593488462288754
1103	45	0.39859223804224797
1103	46	0.4939741249180081
1103	47	0.5264920827252233
1103	48	0.5043351508889202
1103	49	0.49441902561986595
1103	50	0.5494052281521995
1104	1	0.5815828783404946
1104	2	0.7358936206896871
1104	3	0.6027854130421346
1104	4	0.6552708967507854
1104	5	0.7369308852678458
1104	6	0.6319900261566147
1104	7	0.6136362742533663
1104	8	0.7800850195798432
1104	9	0.7854797971193435
1104	10	0.7373307124678732
1104	11	0.7202551416630101
1104	12	0.8113080049746486
1104	13	0.623404351806407
1104	14	0.8748262354028
1104	15	0.8309531798783425
1104	16	0.9467380216229202
1104	17	0.9843127020229261
1104	18	0.8482781033606344
1104	19	0.8433769635622639
1104	20	0.8204269622055946
1104	21	0.7610223888284153
1104	22	0.8018728287734973
1104	23	0.8288339054872722
1104	24	0.7714854504350654
1104	25	0.7585005460035248
1104	26	0.7001143706503825
1104	27	0.8952016467611017
1104	28	0.7479078165213053
1104	29	0.8311205378291078
1104	30	0.7202071851403318
1104	31	0.9567722006837087
1104	32	0.8883091399898007
1104	33	0.8208277333327629
1104	34	0.851416488884972
1104	35	0.8090683458459864
1104	36	0.8195432553896254
1104	37	0.7527290688636228
1104	38	0.8402671219589237
1104	39	1.0532884925700188
1104	40	1.0618610794328447
1104	41	0.9233900352013507
1104	42	0.8083707817062731
1104	43	0.9015662558237736
1104	44	0.9539024506154057
1104	45	0.9809891508031114
1104	46	0.9423296671099557
1104	47	0.8566875133472962
1104	48	0.8947817359560624
1104	49	1.0360801920188543
1104	50	1.1429881575214296
1105	1	0.20955604695857133
1105	2	0.15282839360945744
1105	3	0.09426121976808588
1105	4	0.21336144978191385
1105	5	0.1150461790286954
1105	6	0.039273982966719245
1105	7	0.07041293367251455
1105	8	0.16827491030203845
1105	9	0.21954497403775974
1105	10	0.11278281744790775
1105	11	0.15835920955559146
1105	12	0.13173778794696034
1105	13	0.24588754039118754
1105	14	0.2959526910323689
1105	15	0.12714183327576478
1105	16	0.12103778862993408
1105	17	0.2291862510059417
1105	18	0.046265057829273815
1105	19	-0.05909419771835189
1105	20	0.2389437061618196
1105	21	0.16185882268084129
1105	22	0.17747686063028567
1105	23	0.12843226502294414
1105	24	0.17387502669457433
1105	25	0.14060139777420488
1105	26	0.25195747141163594
1105	27	0.30637963068279817
1105	28	0.26461371895253905
1105	29	0.2805986531516922
1105	30	0.23719719371396764
1105	31	0.07653929995278475
1105	32	0.1917359415052573
1105	33	0.06517573640243057
1105	34	0.214413472833769
1105	35	0.1179548573339062
1105	36	0.22378189993666098
1105	37	0.2924807217530869
1105	38	0.2678314429553339
1105	39	0.29354095640882605
1105	40	0.3097600489246588
1105	41	0.25216282351215114
1105	42	0.25600040531391954
1105	43	0.3424014039193253
1105	44	0.33651054211977893
1105	45	0.3963373401057311
1105	46	0.2946038423661115
1105	47	0.2454607513847087
1105	48	0.2556432391845789
1105	49	0.282634882523336
1105	50	0.19355299685659233
1106	1	0.07844040930604379
1106	2	-0.02038997733720649
1106	3	0.043996500690756335
1106	4	-0.11552831840776889
1106	5	-0.06208975561077068
1106	6	-0.13621275182099168
1106	7	0.007596211229074612
1106	8	0.10242450723241348
1106	9	0.09676464350116021
1106	10	0.07858905422936857
1106	11	-0.08843030533973462
1106	12	0.10436207355376581
1106	13	0.12582559612709332
1106	14	0.06847415417650637
1106	15	0.06899795985049906
1106	16	-0.06944277402349996
1106	17	0.1361609020643968
1106	18	-0.20764735080781804
1106	19	-0.01420689838237621
1106	20	-0.1500186229202214
1106	21	0.06895106473996293
1106	22	0.05835926684310522
1106	23	-0.08774847959001875
1106	24	-0.15546434594907765
1106	25	-0.14630935845853799
1106	26	-0.024942863249009406
1106	27	0.019155391332169813
1106	28	0.07761922789586606
1106	29	-0.01573819212139682
1106	30	0.09822316126219022
1106	31	0.007831045454651075
1106	32	0.06104831280302392
1106	33	-0.02373754998738775
1106	34	0.04379715109141813
1106	35	0.0702356211738362
1106	36	0.1050981667846358
1106	37	0.057602590056738734
1106	38	0.19042842069942623
1106	39	0.03816576588075549
1106	40	0.12595486544647372
1106	41	0.08531972390253628
1106	42	0.02531723503825134
1106	43	0.016289932109117444
1106	44	0.11992547394764941
1106	45	0.11559467770057452
1106	46	0.10720321651448117
1106	47	0.07394048365619454
1106	48	0.07803262144171441
1106	49	0.11395017409050112
1106	50	0.2054820744360022
1107	1	-0.11630362289118068
1107	2	-0.3139331795460407
1107	3	-0.18675722229760439
1107	4	-0.2650632421725163
1107	5	-0.30216081515915505
1107	6	-0.19146640814496152
1107	7	-0.22584566110895565
1107	8	-0.18159975726507938
1107	9	-0.2281322611942258
1107	10	-0.3338783254447577
1107	11	-0.3591663806899277
1107	12	-0.44618487593070455
1107	13	-0.23736333343042304
1107	14	-0.4715300399850335
1107	15	-0.3611542426538037
1107	16	-0.3470569393016021
1107	17	-0.20757803884951187
1107	18	-0.1278532265199317
1107	19	-0.17724688830566748
1107	20	-0.22566675822682214
1107	21	-0.22090174430977783
1107	22	-0.5079811128156138
1107	23	-0.6222072686029279
1107	24	-0.5369879253778578
1107	25	-0.5604893764277676
1107	26	-0.2721940459556649
1107	27	-0.39718301184467464
1107	28	-0.34898569213702046
1107	29	-0.22922925540834882
1107	30	-0.3146729474804886
1107	31	-0.41542055532816846
1107	32	-0.340269632111422
1107	33	-0.36377256386630563
1107	34	-0.3636096979877763
1107	35	-0.3166538050501231
1107	36	-0.5542344791349976
1107	37	-0.5103222580723994
1107	38	-0.40502379500375696
1107	39	-0.27780836163649525
1107	40	-0.27275841135277545
1107	41	-0.2684638989016399
1107	42	-0.3427120651330675
1107	43	-0.4683349148810336
1107	44	-0.39291422131234555
1107	45	-0.30130504632895594
1107	46	-0.2746705687029092
1107	47	-0.17625974400771938
1107	48	-0.3454853688983164
1107	49	-0.23788215860700745
1107	50	-0.07271947445579485
1108	1	-0.1487592572896297
1108	2	-0.0834214062166474
1108	3	-0.008614728303850683
1108	4	-0.13200461856632018
1108	5	-0.04806652774153264
1108	6	-0.08166275563358028
1108	7	-0.2590939883139427
1108	8	-0.1940675165686585
1108	9	-0.2923115004727208
1108	10	-0.24398134062337107
1108	11	-0.22176403307881998
1108	12	-0.3437988124393101
1108	13	-0.28821564293800606
1108	14	-0.43086179801100144
1108	15	-0.35784082548497054
1108	16	-0.27643072361318133
1108	17	-0.42554414028163023
1108	18	-0.33482493068155605
1108	19	-0.312380273890831
1108	20	-0.40378269991874305
1108	21	-0.22129712165093451
1108	22	-0.3467640638345628
1108	23	-0.6021199677354971
1108	24	-0.42802987646425095
1108	25	-0.23907608849305717
1108	26	-0.4000588842734629
1108	27	-0.3772945625223585
1108	28	-0.4803765598507326
1108	29	-0.6163056105254228
1108	30	-0.614553481503485
1108	31	-0.5550961288385068
1108	32	-0.41537213950148555
1108	33	-0.35348810149808685
1108	34	-0.4052692905010389
1108	35	-0.3848061989824032
1108	36	-0.6012099587625954
1108	37	-0.50604384885375
1108	38	-0.3754385225771415
1108	39	-0.4158438944307221
1108	40	-0.352989389244982
1108	41	-0.2411809242375753
1108	42	-0.22245765919527472
1108	43	-0.2747465399051663
1108	44	-0.3029494021432093
1108	45	-0.29565522041127323
1108	46	-0.1786072478588494
1108	47	-0.20354407535873875
1108	48	-0.09961706211340626
1108	49	-0.10100174784871285
1108	50	-0.1477010428944503
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1109	2	-0.20053241264696808
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2210	32	-0.22742146997802593
2210	33	-0.22665442003048497
2210	34	-0.11989182629312722
2210	35	-0.24492644724845292
2210	36	-0.36028535597931466
2210	37	-0.23922732009607017
2210	38	-0.23950329646915947
2210	39	-0.1059842239181421
2210	40	-0.2982598432539021
2210	41	-0.2163864429875204
2210	42	-0.06885573952613555
2210	43	-0.2541042695318403
2210	44	-0.16665632986575574
2210	45	-0.1272336880825975
2210	46	-0.23663286336920208
2210	47	-0.14444268013221162
2210	48	-0.018196973122517335
2210	49	-0.057726267557892896
2210	50	0.011686928095357985
2211	1	0.004705937028585794
2211	2	-0.06338188678630274
2211	3	-0.04534165066364082
2211	4	0.02761626941801154
2211	5	-0.25606501839742535
2211	6	0.06763341674256651
2211	7	0.00955807097160033
2211	8	0.1028202975439072
2211	9	0.11791487162586378
2211	10	-0.034454934234929624
2211	11	-0.0029373021420369128
2211	12	-0.1724722672187582
2211	13	-0.11207142186140118
2211	14	-0.41999690644713894
2211	15	-0.2092907158016999
2211	16	-0.1635399754301261
2211	17	-0.040123184307937265
2211	18	-0.21427578623072918
2211	19	-0.11707985390156495
2211	20	-0.18458834923434608
2211	21	-0.06633205352136429
2211	22	-0.20521361125271653
2211	23	-0.19470688908184286
2211	24	-0.013347052731340625
2211	25	-0.10199155784348335
2211	26	-0.16519906190181644
2211	27	-0.0897855411411328
2211	28	-0.039190061888206174
2211	29	-0.08795276837831523
2211	30	-0.3295262931390113
2211	31	-0.27821782438097387
2211	32	-0.03897834532623534
2211	33	-0.0913236882703714
2211	34	-0.06928943935936616
2211	35	0.02472330677628065
2211	36	0.02855476951856417
2211	37	0.045606049235999535
2211	38	-0.0032879138460657487
2211	39	-0.02060137854891053
2211	40	-0.0020971395892317446
2211	41	0.010199156454284264
2211	42	-0.1150027390434154
2211	43	-0.08102729070054693
2211	44	-0.22236860475888065
2211	45	-0.07237523560310777
2211	46	-0.10583809338180927
2211	47	-0.22199676642384603
2211	48	-0.2189717191139735
2211	49	-0.08953260207942648
2211	50	0.0062061806552549115
2212	1	-0.31586138497411653
2212	2	-0.40709555549414844
2212	3	-0.3768559341868922
2212	4	-0.2879684584688924
2212	5	-0.3149785163126957
2212	6	-0.2808579943979552
2212	7	-0.22794644972989886
2212	8	-0.29372368533139337
2212	9	-0.3590512074916177
2212	10	-0.30418400930413725
2212	11	-0.33771595818245004
2212	12	-0.3685476355967623
2212	13	-0.197982265107143
2212	14	-1.0022554489161735
2212	15	-0.6498617159039526
2212	16	-0.6408010835747788
2212	17	-0.5412662590666173
2212	18	-0.45169095798898695
2212	19	-0.4927094385082711
2212	20	-0.4788129992231731
2212	21	-0.5816180600804088
2212	22	-0.5327168964536
2212	23	-0.6178024193151614
2212	24	-0.5513758564637854
2212	25	-0.34708505825815905
2212	26	-0.5091290615609907
2212	27	-0.6489585651922098
2212	28	-0.6909537981935543
2212	29	-0.6841709293853455
2212	30	-0.6987394401910407
2212	31	-0.4061369904511949
2212	32	-0.44391935372195945
2212	33	-0.5151927081633545
2212	34	-0.48049280276127604
2212	35	-0.420587921036784
2212	36	-0.589546190290271
2212	37	-0.5734663880232915
2212	38	-0.6393117982978822
2212	39	-0.615418240434142
2212	40	-0.5468588299813
2212	41	-0.5370265529408229
2212	42	-0.4205697344195194
2212	43	-0.49041976995739134
2212	44	-0.4596758477274143
2212	45	-0.2784856145657031
2212	46	-0.4342404301347784
2212	47	-0.284048009877111
2212	48	-0.36655759350758643
2212	49	-0.28324986841359845
2212	50	-0.29739643974215113
2213	1	-0.5577304328589889
2213	2	-0.6061736870649668
2213	3	-0.5829948466454447
2213	4	-0.7892402898688928
2213	5	-0.5603633592829169
2213	6	-0.5350551775751704
2213	7	-0.7556289021781062
2213	8	-0.7027148215876124
2213	9	-0.6691707103329989
2213	10	-0.8023787204579236
2213	11	-0.8146312181124102
2213	12	-0.846081715468145
2213	13	-0.6391398270612427
2213	14	-0.8112493065380448
2213	15	-0.9798475368911213
2213	16	-0.8128695447611207
2213	17	-0.8001394229588854
2213	18	-0.7275032274869844
2213	19	-0.8600207664602806
2213	20	-0.757957313476247
2213	21	-0.9410408002769799
2213	22	-0.7661495945224956
2213	23	-0.6915635234174005
2213	24	-0.8142476833979515
2213	25	-0.8493137869477749
2213	26	-0.9412114505571836
2213	27	-1.0746572583784726
2213	28	-1.096605911834061
2213	29	-1.0121823647033708
2213	30	-1.2132351315946899
2213	31	-0.8902001382292539
2213	32	-1.0229024561346272
2213	33	-1.1025959950148305
2213	34	-1.0952433721547052
2213	35	-1.1979873049382057
2213	36	-1.3334337006640204
2213	37	-1.1914829450910247
2213	38	-1.1274052420860983
2213	39	-1.2715339166167503
2213	40	-1.2224375039644855
2213	41	-1.11890081561355
2213	42	-1.1269015847248696
2213	43	-1.0895415596763023
2213	44	-1.033370196962828
2213	45	-1.0778911938769973
2213	46	-1.058525315716473
2213	47	-1.1215803871504981
2213	48	-1.295578446146333
2213	49	-1.2515152559918867
2213	50	-1.1828279925115233
2214	1	-0.4149640739107703
2214	2	-0.593219842385885
2214	3	-0.5615871684634968
2214	4	-0.5792977623028435
2214	5	-0.3070090769410392
2214	6	-0.4789381237944461
2214	7	-0.4702165649046286
2214	8	-0.5171853175479946
2214	9	-0.724345292261944
2214	10	-0.4815279402747237
2214	11	-0.6308207938510648
2214	12	-0.6250605563912401
2214	13	-0.4911317121283858
2214	14	-0.63706354939508
2214	15	-0.4979851435642004
2214	16	-0.5573990655928682
2214	17	-0.2684220479704109
2214	18	-0.545484222583795
2214	19	-0.5174365853749023
2214	20	-0.4851220545533721
2214	21	-0.5497862607042237
2214	22	-0.44196696783176037
2214	23	-0.5006863395655472
2214	24	-0.7043783183370707
2214	25	-0.7409248533354926
2214	26	-0.6998343451612499
2214	27	-0.7556303697399471
2214	28	-0.5980830627021732
2214	29	-0.6424367564831925
2214	30	-0.5260472107142888
2214	31	-0.48612514571620835
2214	32	-0.5913977517266034
2214	33	-0.5133766052495048
2214	34	-0.5884053079462959
2214	35	-0.7031553868695326
2214	36	-0.7158015369187964
2214	37	-0.6154495427765383
2214	38	-0.6210369681240415
2214	39	-0.706474556458943
2214	40	-0.5439638057127247
2214	41	-0.49805373859408064
2214	42	-0.3099015943998005
2214	43	-0.5951764848918586
2214	44	-0.49272764133947433
2214	45	-0.4228546854644222
2214	46	-0.4244607799147211
2214	47	-0.4910827319871025
2214	48	-0.4480339161584368
2214	49	-0.4162795751974855
2214	50	-0.40212184255741334
2215	1	-0.09573573808455649
2215	2	-0.19264092902589525
2215	3	-0.19687367667653177
2215	4	-0.09568389148227396
2215	5	-0.015151914925503718
2215	6	-0.14150966181619395
2215	7	-0.028514558342926932
2215	8	0.0281354108484706
2215	9	0.07765360150148837
2215	10	-0.018778884650402006
2215	11	-0.09798902073479354
2215	12	-0.040153210120585925
2215	13	0.03947988782721268
2215	14	-0.04925425713623355
2215	15	-0.13189912276259008
2215	16	-0.19607390455010432
2215	17	0.02819854047814374
2215	18	-0.021604688152827123
2215	19	-0.0755730387573621
2215	20	-0.01744049283480109
2215	21	-0.001166874742523305
2215	22	-0.028051775879823992
2215	23	-0.12540135852364642
2215	24	-0.15320862074903374
2215	25	-0.1401313980979637
2215	26	-0.241653648732715
2215	27	-0.26496183453448197
2215	28	-0.08913579694809215
2215	29	-0.1478142597123906
2215	30	-0.06377908566335577
2215	31	-0.057154163892754184
2215	32	-0.18837247704434645
2215	33	-0.13082488836906947
2215	34	-0.07809192986299962
2215	35	-0.1416463236221901
2215	36	-0.10063390422013896
2215	37	-0.05959181480934461
2215	38	-0.05165960477858533
2215	39	-0.11012770381106662
2215	40	-0.21526283801239288
2215	41	-0.15361296235481348
2215	42	-0.14216430376822586
2215	43	-0.2139462843768527
2215	44	-0.24019641935231562
2215	45	-0.1978982066333863
2215	46	-0.24746180594272005
2215	47	-0.13359250299485126
2215	48	-0.14986770860561194
2215	49	-0.30967436754391997
2215	50	-0.2763195162522756
2216	1	-0.21333803817291397
2216	2	-0.2828044428826999
2216	3	-0.2850562261372893
2216	4	-0.23431543021976964
2216	5	-0.21370113442469574
2216	6	-0.4242304962045118
2216	7	-0.34916591845226463
2216	8	-0.30711040444539606
2216	9	-0.27148603292796025
2216	10	-0.3054054691967849
2216	11	-0.4590523477204087
2216	12	-0.3599012094177496
2216	13	-0.32699669295996614
2216	14	-0.5257326871586017
2216	15	-0.4545529658314251
2216	16	-0.5159085540236958
2216	17	-0.48529124275923863
2216	18	-0.4871180750029822
2216	19	-0.6110471497789121
2216	20	-0.5716922468333294
2216	21	-0.6597285823384382
2216	22	-0.6388458328609801
2216	23	-0.6832435712225546
2216	24	-0.766276315531826
2216	25	-0.8288869486831345
2216	26	-0.8610021462134156
2216	27	-0.9000924443611424
2216	28	-0.9056225035021086
2216	29	-0.9770125733271797
2216	30	-0.8109162266619876
2216	31	-1.1076899604157333
2216	32	-0.9505221930516612
2216	33	-1.022249494757368
2216	34	-0.9230641881938233
2216	35	-0.824376100518144
2216	36	-0.9878757940254062
2216	37	-1.0409766867252053
2216	38	-1.0811272093404796
2216	39	-1.0957168632300096
2216	40	-1.2110115342806438
2216	41	-1.1487106566328826
2216	42	-1.086197190415973
2216	43	-1.1378844453658665
2216	44	-1.362738931342502
2216	45	-1.238775833128507
2216	46	-1.4109901655245132
2216	47	-1.2653537560516916
2216	48	-1.517991532807219
2216	49	-1.3482843248900416
2216	50	-1.3267795527720523
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152401.0
3	7404.0
4	13384.0
5	10608.0
6	8704.0
7	7371.0
8	6676.0
9	5998.0
10	5905.0
11	5968.0
12	6198.0
13	6275.0
14	7025.0
15	7340.0
16	7596.0
17	8325.0
18	8892.0
19	10145.0
20	12559.0
21	16580.0
22	20464.0
23	22710.0
24	24161.0
25	25413.0
26	27812.0
27	31663.0
28	35968.0
29	40865.0
30	48210.0
31	57389.0
32	69635.0
33	86398.0
34	110392.0
35	140642.0
36	181199.0
37	244197.0
38	373854.0
39	594904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.379439250810144	16.834805955597183	12.918315294544186	24.867439499048487
2	16.274939913907225	19.01848356830133	39.234973168486874	25.471603349304566
3	20.343332426774996	23.756687909533643	27.32987534414506	28.570104319546296
4	13.626356753127796	15.446454933070452	35.26098215062344	35.666206163178316
5	15.114402637504073	37.05551939540593	32.54503299738422	15.28504496970578
6	33.91134827475153	34.514194374497684	16.913978200556027	14.660479150194764
7	30.15194360135641	31.271132756568605	19.718504743696705	18.85841889837828
8	27.497787617844043	34.30669984423523	18.877211973967363	19.318300563953354
9	27.498193722727255	16.295600847419575	18.091509884520715	38.11469554533245
10	15.906111541010798	28.805428490268486	31.610034185187725	23.67842578353299
11	36.43987758956915	22.751924601182473	21.391745213903985	19.41645259534439
12	24.717259238184727	24.423813778670528	28.16484540538864	22.69408157775611
13	29.04734674785748	21.35197473011566	24.558423954635675	25.042254567391186
14	23.041911973590118	20.764435149176	25.61983875865717	30.573814118576713
15	25.039122033806986	27.99117233857926	22.548617851402394	24.421087776211355
16	24.781718698659354	27.22639580233761	23.98830989393596	24.003575605067073
17	23.858751287782436	27.084534039089807	25.078450644666745	23.978264028461012
18	23.614487633085762	26.760095415204717	25.930845201728708	23.694571749980817
19	24.789670011536003	26.195217253223017	26.159458093612255	22.85565464162872
20	25.080778461471887	25.550201849199116	25.925324165037487	23.443695524291506
21	25.378967115832534	25.452129234022834	25.815367719256706	23.35353593088793
22	25.079655802012667	25.15804593008254	25.60258328134544	24.159714986559358
23	24.6186463854984	25.311267004268874	25.71876533272159	24.351321277511136
24	24.28326295552501	25.49029374144433	25.83637863818839	24.390064664842274
25	24.593593610350435	25.35102120642117	25.77270802719733	24.282677156031067
26	24.217550036867767	25.694220456996998	25.941026271243878	24.14720323489136
27	24.312652485327117	25.78978009452049	25.662688575562502	24.234878844589893
28	24.19865264045068	25.927156996216322	25.436658665919676	24.437531697413327
29	24.304266025154213	25.98521483644535	25.645636229121855	24.064882909278577
30	24.640601249582023	25.739895374145828	25.634191660552673	23.985311715719476
31	24.64593640672138	25.502946431158986	25.554172740011584	24.296944422108048
32	24.384006935547006	25.857688224468127	25.493074044882686	24.265230795102177
33	24.48925196413294	25.407643301702578	25.617485517989042	24.48561921617544
34	24.57157877293532	25.688457585625063	25.67347825057693	24.066485390862688
35	24.619268620223767	25.69168411642972	25.54424892546196	24.144798337884556
36	24.529186017749254	25.963485945991398	25.42909809600858	24.07822994025077
37	24.55889778429433	25.517862675313257	25.731755155155728	24.191484385236684
38	24.69118065416437	25.514467622590004	25.601975790767316	24.192375932478306
39	24.61805218245983	25.911960364775982	25.444683996600737	24.025303456163453
40	24.866382410398458	25.366933044668727	25.685966214158775	24.080718330774047
41	24.37663328303818	25.732235247446845	25.577268495712396	24.31386297380258
42	24.71208709765756	25.618708186361953	25.518802337829506	24.150402378150982
43	24.31260710383874	25.442913526416465	25.58395696216273	24.660522407582064
44	24.372600856855787	25.650701898582817	25.537942680935082	24.438754563626315
45	24.64997939254949	25.669007618635664	25.356019211856832	24.324993776958014
46	24.54712795081442	25.425348593039278	25.29415821274224	24.733365243404062
47	24.313994343988345	25.700859828034396	25.496900619876023	24.488245208101237
48	24.404427441979088	25.655700076511096	25.511859219586842	24.428013261922978
49	24.55211992217594	25.576702727759425	25.31314064479062	24.558036705274016
50	24.784542912686018	25.65510380298082	25.34289505770822	24.217458226624945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1563.0
1	1175.0
2	787.0
3	781.5
4	776.0
5	819.0
6	862.0
7	886.5
8	911.0
9	1089.0
10	1267.0
11	1526.5
12	1786.0
13	2696.5
14	3607.0
15	4904.5
16	6202.0
17	6458.5
18	6715.0
19	6674.5
20	6634.0
21	6889.0
22	7144.0
23	8286.0
24	9428.0
25	11407.5
26	13387.0
27	16474.5
28	19562.0
29	23894.0
30	28226.0
31	34327.5
32	40429.0
33	48975.0
34	57521.0
35	67343.0
36	77165.0
37	86814.0
38	96463.0
39	107509.0
40	118555.0
41	129932.0
42	141309.0
43	150551.5
44	159794.0
45	169259.0
46	178724.0
47	186727.0
48	194730.0
49	195890.5
50	197051.0
51	195245.5
52	193440.0
53	187724.5
54	182009.0
55	173464.0
56	164919.0
57	153517.0
58	142115.0
59	129097.5
60	116080.0
61	103254.0
62	90428.0
63	77835.0
64	65242.0
65	54893.0
66	44544.0
67	37183.0
68	29822.0
69	24900.0
70	19978.0
71	16267.0
72	12556.0
73	10487.0
74	8418.0
75	6449.5
76	4481.0
77	3571.0
78	2661.0
79	2177.0
80	1693.0
81	1318.5
82	944.0
83	763.0
84	582.0
85	449.0
86	316.0
87	247.0
88	178.0
89	139.0
90	100.0
91	72.5
92	45.0
93	36.0
94	27.0
95	20.5
96	14.0
97	13.5
98	13.0
99	20.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08040861118703671
2	0.09154587696788959
3	0.09823639560547154
4	0.10039857540908034
5	0.09170906034929402
6	0.05511518706934886
7	0.050668439926077925
8	0.05637985827523325
9	0.05874601730559759
10	0.04267245423726048
11	0.045364980030433696
12	0.05593110397637105
13	0.04891421857598022
14	0.050505256544673485
15	0.04911819780273577
16	0.05283061972968673
17	0.052300273740122305
18	0.05327937402854893
19	0.06152013478947304
20	0.06645643207695728
21	0.0764922100333302
22	0.07922553167185453
23	0.057277366872957654
24	0.07469719283788138
25	0.08236681176388996
26	0.07898075659974788
27	0.07400366346691253
28	0.09350407754474284
29	0.08232601591853886
30	0.07877677737299234
31	0.05372812832741113
32	0.05070923577142904
33	0.05278982388433562
34	0.04846546427711802
35	0.05466643277048666
36	0.05401369924486891
37	0.057154979336904325
38	0.047853526596851374
39	0.05254504881222897
40	0.05417688262627335
41	0.0418973331755894
42	0.03765456525907402
43	0.0375729735683718
44	0.03573716052757187
45	0.026925257931732234
46	0.0241919362932079
47	0.030188925559820987
48	0.024681486437421213
49	0.02341681523153682
50	0.02553819918979451
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2451230.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.388164078315384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21079729098076	46.62618295425296
2	11.939477218047044	14.420062185054668
3	4.590347714383077	8.316080128580557
4	2.1639062816477233	5.226973103449603
5	1.1705067687491144	3.5342377403000147
6	0.7382684099768557	2.674960432531163
7	0.4937750230180605	2.087271697545202
8	0.325971426518051	1.5747852791531673
9	0.24659021765969977	1.3402017471727308
>10	1.0621243407551584	10.8736030370534
>50	0.04447124747227971	1.8108942643288575
>100	0.01328859591231252	1.2370570098963234
>500	3.3961777133246823E-4	0.1381479079564167
>1k	1.3584710852911465E-4	0.13954251272494939
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.159169070221889E-5
2	4.0795845351109444E-5	0.0	0.0	0.0	8.159169070221889E-5
3	4.0795845351109444E-5	0.0	0.0	0.0	8.159169070221889E-5
4	4.0795845351109444E-5	0.0	0.0	0.0	8.159169070221889E-5
5	4.0795845351109444E-5	0.0	0.0	0.0	8.159169070221889E-5
6	4.0795845351109444E-5	0.0	0.0	0.0	1.6318338140443778E-4
7	4.0795845351109444E-5	0.0	0.0	0.0	1.6318338140443778E-4
8	4.0795845351109444E-5	0.0	0.0	0.0	1.6318338140443778E-4
9	4.0795845351109444E-5	0.0	0.0	0.0	1.6318338140443778E-4
10	4.0795845351109444E-5	0.0	0.0	0.0	2.0397922675554722E-4
11	4.0795845351109444E-5	0.0	0.0	0.0	2.0397922675554722E-4
12	4.0795845351109444E-5	0.0	0.0	0.0	3.2636676280887555E-4
13	4.0795845351109444E-5	0.0	0.0	0.0	4.0795845351109444E-4
14	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	4.0795845351109444E-4
15	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	4.895501442133133E-4
16	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	5.303459895644228E-4
17	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	5.303459895644228E-4
18	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	5.711418349155322E-4
19	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	6.119376802666416E-4
20	4.0795845351109444E-5	0.0	0.0	4.0795845351109444E-5	7.751210616710794E-4
21	8.159169070221889E-5	0.0	0.0	8.159169070221889E-5	8.567127523732984E-4
22	8.159169070221889E-5	0.0	0.0	8.159169070221889E-5	8.975085977244077E-4
23	1.2238753605332833E-4	0.0	0.0	2.4477507210665666E-4	0.0010606919791288456
24	1.2238753605332833E-4	0.0	0.0	3.6716260815998497E-4	0.001183079515182174
25	1.2238753605332833E-4	0.0	0.0	4.0795845351109444E-4	0.001183079515182174
26	1.2238753605332833E-4	0.0	0.0	5.711418349155322E-4	0.001183079515182174
27	1.2238753605332833E-4	0.0	0.0	6.935293709688606E-4	0.0012646712058843926
28	1.2238753605332833E-4	0.0	0.0	0.0012238753605332833	0.0012646712058843926
29	1.2238753605332833E-4	0.0	0.0	0.002651729947822114	0.0012646712058843926
30	1.2238753605332833E-4	0.0	0.0	0.0050994806688886805	0.0012646712058843926
31	1.2238753605332833E-4	0.0	0.0	0.011300449162257316	0.0013870587419377212
32	1.2238753605332833E-4	0.0	0.0	0.019010863933617	0.0013870587419377212
33	1.2238753605332833E-4	0.0	0.0	0.028883458508585487	0.0015094462779910495
34	1.2238753605332833E-4	0.0	0.0	0.04185653733023829	0.0016726296593954871
35	1.2238753605332833E-4	0.0	0.0	0.056012695667073266	0.0016726296593954871
36	1.2238753605332833E-4	0.0	0.0	0.07551310974490358	0.0016726296593954871
37	1.2238753605332833E-4	0.0	0.0	0.09872594574968485	0.0016726296593954871
38	1.2238753605332833E-4	0.0	0.0	0.12671189566054594	0.0016726296593954871
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5200	0.0	22.470867	1
GGTATCA	2540	0.0	21.572193	1
CGTGCCG	245	0.0	18.853275	10
TATCAAC	6465	0.0	17.767315	2
ATCAACG	6500	0.0	17.638151	3
TCAACGC	6525	0.0	17.46904	4
CAACGCA	6610	0.0	17.309921	5
GCGTATC	90	2.212412E-4	17.115288	1
AACGCAG	6730	0.0	17.028406	6
GTACATG	10235	0.0	14.534079	1
GCACCGT	395	0.0	14.478636	6
ACGCAGA	7950	0.0	14.249231	7
GAGTACG	170	7.578619E-7	14.23877	1
ACCGTGC	510	0.0	14.233828	8
TACATGG	10275	0.0	14.0703	2
AGAGTAC	7595	0.0	13.756793	11
CGCAGAG	8125	0.0	13.726585	8
GTCTTAC	345	0.0	13.394573	1
GTATAGA	445	0.0	13.351572	1
GTGTAAG	660	0.0	13.336588	1
>>END_MODULE