FastQCFastQC Report
Mon 6 Feb 2017
SRR2077150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451230
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.10092892139864477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27750.025.9252011
GTATCAA56650.022.0201641
TACCGTA1152.0206244E-719.1330347
TATCAAC75600.016.3553852
TATACCG1501.8374158E-716.1351975
ATCAACG76900.015.9361713
CGTGCCG1805.2568794E-915.89266910
TCAACGC77250.015.8070054
TATGCGA1401.583483E-615.71834712
CAACGCA78300.015.567575
AACGCAG79600.015.3682956
GTGGTAT10950.014.8680831
ATACGCG1057.181768E-414.66147941
AGCACCG4950.013.7793695
ACCGTAT1606.339642E-613.7518698
GTACATG96050.013.628781
GCACCGT4050.013.5815386
TACATGG98000.013.3129672
CGGACCA2502.071829E-913.2026029
CGCCCGA3450.012.75405526
Produced by FastQC (version 0.11.5)