FastQCFastQC Report
Mon 6 Feb 2017
SRR2077149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392136
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63080.18595952520771572No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC43010.1267932653643604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG600.00740232418.33868424
TATCATA10400.018.1976172
TCATACT11100.017.843574
TAGGCCG1509.582436E-917.6046165
TATAGGC12950.017.5026595
GTCCTAG5650.016.748251
CTTATAG14750.016.4122943
TTATAGG14000.016.3481084
GTACATG109650.015.714571
ATAGGCC14850.015.5541266
ATCGTAA3400.015.52660910
TACATGG109650.015.2328982
TCTTATA16450.014.9830942
GTGTAGA9100.014.7515551
ACGCGTC900.00436425414.66510338
TAGGCCT16000.014.5738757
AATATCG9550.014.50940133
ATATCGA9300.014.42580234
GTCTATA2753.6379788E-1214.4042021
ATACTTG13950.014.3499366
Produced by FastQC (version 0.11.5)