##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077149_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3392136
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.588594325227525	31.0	30.0	34.0	16.0	34.0
2	28.728911812498083	31.0	30.0	34.0	16.0	34.0
3	28.802333397010024	31.0	30.0	34.0	16.0	34.0
4	32.17124490291663	35.0	33.0	37.0	19.0	37.0
5	32.09413331305113	35.0	33.0	37.0	19.0	37.0
6	32.18733918687222	35.0	33.0	37.0	19.0	37.0
7	32.025680868927424	35.0	33.0	37.0	18.0	37.0
8	31.95363481888698	35.0	33.0	37.0	17.0	37.0
9	33.250460771620006	38.0	33.0	39.0	17.0	39.0
10	33.19193511109224	38.0	33.0	39.0	17.0	39.0
11	33.201519632467566	38.0	33.0	39.0	17.0	39.0
12	33.1071286646526	38.0	33.0	39.0	17.0	39.0
13	33.04121414943269	38.0	33.0	39.0	17.0	39.0
14	33.932546926184564	39.0	33.0	41.0	16.0	41.0
15	33.96798123660136	39.0	33.0	41.0	16.0	41.0
16	33.92830859375921	39.0	33.0	41.0	16.0	41.0
17	33.771412172153475	39.0	33.0	41.0	11.0	41.0
18	33.72870515804791	38.0	33.0	41.0	11.0	41.0
19	33.84750640894115	39.0	33.0	41.0	13.0	41.0
20	33.820311449776774	39.0	33.0	41.0	11.0	41.0
21	33.76484610286852	39.0	33.0	41.0	11.0	41.0
22	33.69533562333586	39.0	33.0	41.0	10.0	41.0
23	33.6431154293342	39.0	33.0	41.0	10.0	41.0
24	33.631065204932824	39.0	33.0	41.0	10.0	41.0
25	33.52812387239191	39.0	33.0	41.0	10.0	41.0
26	33.47540428803562	39.0	33.0	41.0	10.0	41.0
27	33.399568000811286	38.0	33.0	41.0	9.0	41.0
28	33.28698259739586	38.0	32.0	40.0	8.0	41.0
29	33.27732113335079	38.0	32.0	40.0	8.0	41.0
30	33.10623041057316	38.0	32.0	40.0	8.0	41.0
31	33.10114423478304	38.0	32.0	40.0	8.0	41.0
32	32.96784621842992	38.0	32.0	40.0	2.0	41.0
33	32.92041415792291	38.0	32.0	40.0	2.0	41.0
34	32.81728297450338	38.0	32.0	40.0	2.0	41.0
35	32.744509064495055	38.0	32.0	40.0	2.0	41.0
36	32.66211230917628	38.0	32.0	40.0	2.0	41.0
37	32.56100050233835	38.0	31.0	40.0	2.0	41.0
38	32.474302032701516	38.0	31.0	40.0	2.0	41.0
39	32.358238289974224	38.0	31.0	40.0	2.0	41.0
40	32.297124289828005	38.0	31.0	40.0	2.0	41.0
41	32.17377103984038	38.0	31.0	40.0	2.0	41.0
42	32.02872408417587	38.0	31.0	40.0	2.0	41.0
43	31.9412788874031	38.0	31.0	40.0	2.0	41.0
44	31.78521674838509	38.0	30.0	40.0	2.0	41.0
45	31.63796262885686	38.0	30.0	40.0	2.0	41.0
46	31.485209024638163	38.0	30.0	40.0	2.0	41.0
47	31.331414778181063	38.0	30.0	40.0	2.0	41.0
48	31.132039812083008	37.0	30.0	40.0	2.0	41.0
49	30.955603489954413	37.0	30.0	40.0	2.0	41.0
50	30.73976161333154	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	2.059523498936592
1101	2	2.4250627573508474
1101	3	2.517487664963614
1101	4	2.788844451217095
1101	5	2.7979306504029893
1101	6	2.758512528717631
1101	7	2.8740350802180643
1101	8	2.8935276654752897
1101	9	3.1564195645741293
1101	10	3.2704140007815283
1101	11	3.2887427384539123
1101	12	3.467638463598959
1101	13	3.370381478920031
1101	14	3.736198930991897
1101	15	3.8421682175726914
1101	16	3.7325809306470745
1101	17	3.9043791491342503
1101	18	3.7885027427831943
1101	19	3.7522387459167703
1101	20	3.9131186174536623
1101	21	3.898122247094726
1101	22	3.868711321872368
1101	23	3.995942787117059
1101	24	3.9390614754385
1101	25	3.9471401465311757
1101	26	4.063303033209891
1101	27	4.032354725798889
1101	28	4.058533733694915
1101	29	4.020069532306103
1101	30	4.157578863738394
1101	31	4.091147734811585
1101	32	4.0730833671689055
1101	33	4.045147430585573
1101	34	4.121641322183059
1101	35	4.1621146793051125
1101	36	4.151398874850571
1101	37	4.300072007887891
1101	38	4.242785566497936
1101	39	4.349535723774764
1101	40	4.3612999689410685
1101	41	4.415709272816102
1101	42	4.342605335361007
1101	43	4.386267460594965
1101	44	4.178971755468634
1101	45	4.243009069764007
1101	46	4.288867554979241
1101	47	4.28561721535748
1101	48	4.165839689764116
1101	49	4.24481793922666
1101	50	4.318927565543262
1102	1	0.961523635994368
1102	2	1.1012903037157145
1102	3	1.103533515503198
1102	4	1.236329314128504
1102	5	1.2924638218292728
1102	6	1.304634682004064
1102	7	1.4125314548948609
1102	8	1.4328550888870097
1102	9	1.5882395214190623
1102	10	1.6124408822348926
1102	11	1.485957057293085
1102	12	1.5746485337196034
1102	13	1.6629180303796076
1102	14	1.8855275110552157
1102	15	1.7737390410905007
1102	16	1.8434242674455277
1102	17	2.1020776662633907
1102	18	2.0270990100980697
1102	19	2.0415399361801576
1102	20	1.9345582969850241
1102	21	1.9081635783535802
1102	22	1.8583045032117766
1102	23	1.779707532158774
1102	24	1.8049466710594686
1102	25	1.8893334552835483
1102	26	1.9471108898111993
1102	27	1.8269777824701094
1102	28	2.010343132014903
1102	29	1.80343862273196
1102	30	1.9479556674712981
1102	31	1.9774576572506604
1102	32	2.1019274298659454
1102	33	2.151136311501581
1102	34	2.144875805919412
1102	35	2.2524577262154892
1102	36	2.2171558598115624
1102	37	2.163896768281532
1102	38	2.0613651620759725
1102	39	2.161175699829542
1102	40	2.1602048845653314
1102	41	2.0932877447379283
1102	42	2.1024217359555024
1102	43	2.0612236329266267
1102	44	1.857674028633479
1102	45	1.9203280021201934
1102	46	1.8983528232625844
1102	47	1.897360601141397
1102	48	1.7756489655298004
1102	49	1.8592198107542153
1102	50	1.804742557125806
1103	1	0.31502041219016164
1103	2	0.4771040028106981
1103	3	0.38614479584446215
1103	4	0.44414876318825947
1103	5	0.4958757745344471
1103	6	0.3425017895240998
1103	7	0.33450002490872066
1103	8	0.5428538590050138
1103	9	0.6946462261303594
1103	10	0.7091574383436239
1103	11	0.6165622551538164
1103	12	0.627935206850438
1103	13	0.5527984462033473
1103	14	0.6555340782414092
1103	15	0.6761684736800717
1103	16	0.4625044052349381
1103	17	0.7072792358230231
1103	18	0.5197009821296561
1103	19	0.5941327810805319
1103	20	0.5970811068199069
1103	21	0.4042708632835641
1103	22	0.4464614003452496
1103	23	0.5016561085970039
1103	24	0.3700735695166202
1103	25	0.5315290653508455
1103	26	0.6124721053012294
1103	27	0.5941170407084542
1103	28	0.5690153454422742
1103	29	0.6143354624894215
1103	30	0.7551658453523089
1103	31	0.7193515945292361
1103	32	0.808037513470218
1103	33	0.7691408460786349
1103	34	0.690049755356128
1103	35	0.6738653464108779
1103	36	0.7840126501776439
1103	37	0.7808586528103234
1103	38	0.6957806707813816
1103	39	0.7911863126241059
1103	40	0.783178653974602
1103	41	0.6944598201499446
1103	42	0.7576772860378114
1103	43	0.7770574577509279
1103	44	0.7672553635861981
1103	45	0.6516562512307615
1103	46	0.6301300112748791
1103	47	0.672289040979603
1103	48	0.5120156809583385
1103	49	0.6495612085183033
1103	50	0.7463997687637764
1104	1	0.29047836810090644
1104	2	0.28809052364732324
1104	3	0.3046133469979786
1104	4	0.2360552172073369
1104	5	0.2695651804588266
1104	6	0.23848350559479314
1104	7	0.3724530071705203
1104	8	0.401144670955631
1104	9	0.4992908803552112
1104	10	0.23057031893608126
1104	11	0.24907497620046115
1104	12	0.40974581666488774
1104	13	0.30979534901268124
1104	14	0.49527849369574284
1104	15	0.40659040534098523
1104	16	0.3539057664143428
1104	17	0.472529159693039
1104	18	0.26216931258579024
1104	19	0.3596816367256963
1104	20	0.36886190480363723
1104	21	0.28723634996196523
1104	22	0.4327883486628039
1104	23	0.3143371538396167
1104	24	0.3240527185655182
1104	25	0.4163200447697548
1104	26	0.2974531532671847
1104	27	0.3749357501875821
1104	28	0.2165835892110195
1104	29	0.343940990675911
1104	30	0.31820133271331486
1104	31	0.3282765754569468
1104	32	0.35126753284136214
1104	33	0.30662795880159166
1104	34	0.4018201055823596
1104	35	0.28770646763846486
1104	36	0.3825004840575943
1104	37	0.4271888337786365
1104	38	0.3918847607212257
1104	39	0.4722806108860098
1104	40	0.3796302008192143
1104	41	0.41348609198050923
1104	42	0.36669061665486424
1104	43	0.42115152152186397
1104	44	0.5460859221097287
1104	45	0.5462455931741275
1104	46	0.49565375551915025
1104	47	0.5116267224757891
1104	48	0.49208762689031005
1104	49	0.5781592743745421
1104	50	0.5605062573729249
1105	1	0.16927564430887543
1105	2	0.15964024489521123
1105	3	0.03692350200522654
1105	4	-0.022165586582282515
1105	5	0.06046546698890154
1105	6	-0.09567479349041719
1105	7	0.0032522303779529693
1105	8	0.13509873581881493
1105	9	0.06944508471990218
1105	10	0.0059834290670011114
1105	11	0.013797654227964529
1105	12	-0.050281481471913025
1105	13	0.04257167992229682
1105	14	0.17184173856103513
1105	15	0.10415765962012102
1105	16	0.021402918676720617
1105	17	-0.009157503862446958
1105	18	0.008144593721475246
1105	19	0.040672799957512495
1105	20	0.042979357044444555
1105	21	0.07707933549495749
1105	22	0.18527453650811054
1105	23	0.013546812562722721
1105	24	0.1629987294747366
1105	25	0.0028149941126471845
1105	26	-8.266626711090908E-4
1105	27	0.14611012205444496
1105	28	0.2106888977953858
1105	29	0.18079146814240232
1105	30	0.15002925409066847
1105	31	0.06279466799963984
1105	32	0.17323199508312115
1105	33	0.17717055945092852
1105	34	0.11471797341722834
1105	35	0.11295322974824984
1105	36	0.14102287204239872
1105	37	0.13195546983526185
1105	38	0.12453062259466208
1105	39	0.2090387564191971
1105	40	0.18908368609879744
1105	41	0.24742811354803962
1105	42	0.17820149827429077
1105	43	0.1897767291432615
1105	44	0.25934358340207453
1105	45	0.2330476465560345
1105	46	0.3108268026234917
1105	47	0.1740260480512319
1105	48	0.2556817477548172
1105	49	0.23537245576163102
1105	50	0.2985731076998519
1106	1	-0.13743792187063164
1106	2	-0.17640236100025675
1106	3	-0.24588188153040136
1106	4	-0.1459578415570988
1106	5	-0.12805075793058762
1106	6	-0.24679625332031918
1106	7	-0.15791431243436804
1106	8	-0.09492999838975535
1106	9	-0.04036021624615671
1106	10	-0.18101648836343287
1106	11	-0.2933713656712982
1106	12	-0.16631847263696642
1106	13	-0.039634083433327305
1106	14	-0.06176721197979873
1106	15	-0.11985191844939891
1106	16	-0.22234871207218276
1106	17	-0.04290830891570607
1106	18	-0.33688141569145813
1106	19	-0.192960921452773
1106	20	-0.20522013102425518
1106	21	-0.09440972403770331
1106	22	-0.1598163725827959
1106	23	-0.23355268376293026
1106	24	-0.26034038372814194
1106	25	-0.2734961280077428
1106	26	-0.2444671547857169
1106	27	-0.12238050629994746
1106	28	-0.17620797281811207
1106	29	-0.2683248723744853
1106	30	-0.25290196546180255
1106	31	-0.3202166440307437
1106	32	-0.21854093861352197
1106	33	-0.30390878990090187
1106	34	-0.18123397109602735
1106	35	-0.1706963450184915
1106	36	-0.10941078331223508
1106	37	-0.29566735239246356
1106	38	-0.23718170504137248
1106	39	-0.29906544637164956
1106	40	-0.12327185844748811
1106	41	-0.24135134314422402
1106	42	-0.22004703611593612
1106	43	-0.2941824814916991
1106	44	-0.22995325418358803
1106	45	-0.13824126435140727
1106	46	-0.3039779203556172
1106	47	-0.26523247725634036
1106	48	-0.18095238650329648
1106	49	-0.1970598784799691
1106	50	-0.18181117105499922
1107	1	-0.42714795634713454
1107	2	-0.5730251154912729
1107	3	-0.47214644331592126
1107	4	-0.6589594746545409
1107	5	-0.60463939883061
1107	6	-0.6817750230535822
1107	7	-0.5339825396163675
1107	8	-0.6772783925106047
1107	9	-0.6069267196030808
1107	10	-0.7176240006116643
1107	11	-0.7078289958654551
1107	12	-0.8530315918530817
1107	13	-0.7291652030123501
1107	14	-1.010952840722311
1107	15	-0.8080428093947845
1107	16	-0.8875862370222833
1107	17	-0.761152547710843
1107	18	-0.7445733046408307
1107	19	-0.8849337031620834
1107	20	-0.7093130361231488
1107	21	-0.7702754471369531
1107	22	-1.0823719886234429
1107	23	-1.0239678534236063
1107	24	-0.9682953828208625
1107	25	-0.9264077592906332
1107	26	-0.6303989276037143
1107	27	-0.6871255616438035
1107	28	-0.835705704627216
1107	29	-0.7438015553721229
1107	30	-0.7200948524151727
1107	31	-0.7437241744245
1107	32	-0.8275843064233541
1107	33	-0.778423034139692
1107	34	-0.8425161340428602
1107	35	-0.7133782539279494
1107	36	-0.8483688671445719
1107	37	-0.787639771191234
1107	38	-0.8375994150758501
1107	39	-0.7321901061430189
1107	40	-0.6462734915449282
1107	41	-0.626143939769154
1107	42	-0.7129180214180586
1107	43	-0.8669224560879627
1107	44	-0.6771544880794345
1107	45	-0.5927359114209025
1107	46	-0.7611181852803135
1107	47	-0.6147044424169295
1107	48	-0.8279718022173199
1107	49	-0.688225184232099
1107	50	-0.641376767135565
1108	1	-0.15248749968710484
1108	2	-0.21741809842509952
1108	3	-0.20368153327006056
1108	4	-0.31573751760927493
1108	5	-0.30558665174404
1108	6	-0.298028503542092
1108	7	-0.31430962077618574
1108	8	-0.2626033564736652
1108	9	-0.28147846048836556
1108	10	-0.36132821422340555
1108	11	-0.2424560980693684
1108	12	-0.4405272317130908
1108	13	-0.42924547746033426
1108	14	-0.48639362062716884
1108	15	-0.4552016572541433
1108	16	-0.4403106662726657
1108	17	-0.5457222524712435
1108	18	-0.5240690491638773
1108	19	-0.45193368486572183
1108	20	-0.5864814905537727
1108	21	-0.5011459989662441
1108	22	-0.5696947443014295
1108	23	-0.7410477062684322
1108	24	-0.5702651463525754
1108	25	-0.4424461559342774
1108	26	-0.5605065147752057
1108	27	-0.6363526454726625
1108	28	-0.7708416095102919
1108	29	-0.7336625863410404
1108	30	-0.7778518619693457
1108	31	-0.7755391133936911
1108	32	-0.5349796809337235
1108	33	-0.6101747162405502
1108	34	-0.6157722633061624
1108	35	-0.5662261278486014
1108	36	-0.7134705132462003
1108	37	-0.7071540297916847
1108	38	-0.5102320713718242
1108	39	-0.5233204649345851
1108	40	-0.5835325280272734
1108	41	-0.5680282166862938
1108	42	-0.39000807038343765
1108	43	-0.421192042223705
1108	44	-0.4781032689682263
1108	45	-0.41666029382211534
1108	46	-0.41984347872207906
1108	47	-0.4293084374363829
1108	48	-0.4775258990045259
1108	49	-0.5214776594702109
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2210	28	-0.3972831609086498
2210	29	-0.5221674377850718
2210	30	-0.3828552837158128
2210	31	-0.510627635897535
2210	32	-0.6267438678529729
2210	33	-0.4830482415909074
2210	34	-0.4671707662578086
2210	35	-0.5028303906093967
2210	36	-0.5460289394634827
2210	37	-0.5424777539196484
2210	38	-0.4945029428295271
2210	39	-0.34805160226622434
2210	40	-0.514399984600491
2210	41	-0.5295086814826071
2210	42	-0.44733426520940966
2210	43	-0.5285343110632219
2210	44	-0.5802723584074059
2210	45	-0.4087586135982413
2210	46	-0.415694878181629
2210	47	-0.4588892140258345
2210	48	-0.45075856902731815
2210	49	-0.3553383326885431
2210	50	-0.2863433429593272
2211	1	-0.09538214006542844
2211	2	-0.046928929559307164
2211	3	-0.16110963289736446
2211	4	-0.15056018121280346
2211	5	-0.33374316297334516
2211	6	-0.11554033522449458
2211	7	-0.12515160414497473
2211	8	-0.03454296546907898
2211	9	-0.0032996155939102323
2211	10	-0.10527998356891999
2211	11	-0.02774788474387435
2211	12	-0.3257789564686817
2211	13	-0.148043831457926
2211	14	-0.4113378316153842
2211	15	-0.20559916424305413
2211	16	-0.2167149119975491
2211	17	-0.06585270558631606
2211	18	-0.2953350038666187
2211	19	-0.23637250291076128
2211	20	-0.30577937688280343
2211	21	-0.09091627085515341
2211	22	-0.20558750168571294
2211	23	-0.2776318945753218
2211	24	-0.12146380786780497
2211	25	-0.20519161581459855
2211	26	-0.1975703624828924
2211	27	-0.16063809557842035
2211	28	-0.12201096796681554
2211	29	-0.14208172341207614
2211	30	-0.4810281316390146
2211	31	-0.32167446695028445
2211	32	-0.045129194904788505
2211	33	-0.2423986248918979
2211	34	-0.3232938740700817
2211	35	-0.1582543090961792
2211	36	-0.10981631281753579
2211	37	-0.0739805936755431
2211	38	-0.1921231487484789
2211	39	-0.15061980848285828
2211	40	-0.16013499994635794
2211	41	-0.2305612793796783
2211	42	-0.26531233230466356
2211	43	-0.2619450437554214
2211	44	-0.36889145287585023
2211	45	-0.14429581305096661
2211	46	-0.272369853248712
2211	47	-0.3855547072305434
2211	48	-0.39883196400236187
2211	49	-0.2205965552602649
2211	50	-0.18534171475723582
2212	1	-0.13317260056795632
2212	2	-0.16916029250311837
2212	3	-0.15090060666865668
2212	4	-0.18593947216412943
2212	5	-0.15478922046414567
2212	6	-0.20497186212986662
2212	7	-0.06623655298285769
2212	8	-0.1056878005306956
2212	9	-0.2084734079281887
2212	10	-0.1836484860349188
2212	11	-0.20938753591158843
2212	12	-0.26633257308650116
2212	13	-0.20241555880605233
2212	14	-0.9989032037006353
2212	15	-0.5483089541209551
2212	16	-0.471760568092364
2212	17	-0.3151467229385361
2212	18	-0.2188124657868613
2212	19	-0.19595830187384422
2212	20	-0.16045818930179934
2212	21	-0.25546389434266814
2212	22	-0.29925054353457625
2212	23	-0.42090619755614256
2212	24	-0.5804121795950223
2212	25	-0.34331780259782363
2212	26	-0.4632365994996661
2212	27	-0.4254402391675782
2212	28	-0.44206328150799834
2212	29	-0.3629522130167686
2212	30	-0.37557587639611967
2212	31	-0.24432808932682093
2212	32	-0.2501446383644961
2212	33	-0.2703370425447318
2212	34	-0.20228393713852455
2212	35	-0.29798588934112047
2212	36	-0.5003838436919139
2212	37	-0.3286177744357417
2212	38	-0.558646793492585
2212	39	-0.4903901124561898
2212	40	-0.3746376616233178
2212	41	-0.32985469038396076
2212	42	-0.08651099265032514
2212	43	-0.23189697972914303
2212	44	-0.14341290943406548
2212	45	-0.1491859297891338
2212	46	-0.13137030740648825
2212	47	0.026386810606279454
2212	48	-0.12012524637199107
2212	49	-0.16109829897089867
2212	50	-0.06441930145181729
2213	1	-0.44798754640843086
2213	2	-0.5044769647695375
2213	3	-0.5379680830908917
2213	4	-0.6337705828383946
2213	5	-0.5321959909452012
2213	6	-0.6951454901791436
2213	7	-0.7818896834170843
2213	8	-0.6331861271382699
2213	9	-0.5996960798092204
2213	10	-0.7624822406723979
2213	11	-0.830472178494027
2213	12	-0.8489031511729124
2213	13	-0.6910241243188011
2213	14	-0.75818724097428
2213	15	-1.024564661359598
2213	16	-0.7943225193077694
2213	17	-0.8524530956108194
2213	18	-0.8331429198207232
2213	19	-0.9206640375447108
2213	20	-0.8573114147629113
2213	21	-1.000884223557172
2213	22	-0.8416023672725714
2213	23	-0.9325349124283662
2213	24	-0.8945984997386276
2213	25	-0.8715785179346014
2213	26	-0.8974858383061033
2213	27	-1.0092712557583354
2213	28	-0.9619722053603397
2213	29	-0.9649662693219199
2213	30	-1.1157922914053486
2213	31	-0.9190821129105515
2213	32	-1.0308502021863575
2213	33	-1.0742478949814505
2213	34	-1.1176807792600911
2213	35	-1.17962794737063
2213	36	-1.3691269957863774
2213	37	-1.123362942951342
2213	38	-1.0174599542961502
2213	39	-1.2124069785180573
2213	40	-1.1171833764224317
2213	41	-1.123874058200176
2213	42	-0.9751442327734239
2213	43	-1.0771306335009534
2213	44	-1.0039039309928945
2213	45	-1.1168263989802618
2213	46	-1.090449878786007
2213	47	-1.0999010768882087
2213	48	-1.2580588679093658
2213	49	-1.3047918261595974
2213	50	-1.1410388428635727
2214	1	-0.5387216005026723
2214	2	-0.7973678738248751
2214	3	-0.6816632386023542
2214	4	-0.6258172030480225
2214	5	-0.6177949059980925
2214	6	-0.5884162859822695
2214	7	-0.4888588831859124
2214	8	-0.6331527400721662
2214	9	-0.8831176532438221
2214	10	-0.717495914537821
2214	11	-0.7197943699301561
2214	12	-0.7444626335130735
2214	13	-0.658034736672306
2214	14	-0.7703591617700596
2214	15	-0.5794945583033808
2214	16	-0.5574390447757622
2214	17	-0.466575925554082
2214	18	-0.7362637206003555
2214	19	-0.7082709858160285
2214	20	-0.5078549896492532
2214	21	-0.7253919520949523
2214	22	-0.6482936212939308
2214	23	-0.6258976836028225
2214	24	-0.901701298262708
2214	25	-0.8687583522224145
2214	26	-0.9240415888053803
2214	27	-0.9412856905837828
2214	28	-0.8152226066520427
2214	29	-0.7808077568728677
2214	30	-0.7546887661918973
2214	31	-0.7742513553567534
2214	32	-0.8245155775708994
2214	33	-0.8437522687186672
2214	34	-0.857833093706418
2214	35	-0.9043919484640064
2214	36	-0.8362006901296191
2214	37	-0.7762828059772744
2214	38	-0.7206514927387069
2214	39	-0.6867996037253938
2214	40	-0.7132304866456352
2214	41	-0.6484629381360563
2214	42	-0.6943775622294837
2214	43	-0.8564300718724347
2214	44	-0.6786648363668171
2214	45	-0.5806050928441522
2214	46	-0.6557567157060937
2214	47	-0.569986391886971
2214	48	-0.5938998391915007
2214	49	-0.6197044716465783
2214	50	-0.7381778174413718
2215	1	0.01675062909555436
2215	2	-0.037639213093523693
2215	3	-0.07592026500207538
2215	4	-0.010895678815138865
2215	5	0.10495110678665043
2215	6	0.011846430552346021
2215	7	0.09410951634168896
2215	8	0.12880474970840083
2215	9	0.05371506552416605
2215	10	-0.003855083059711717
2215	11	-0.053956259811464236
2215	12	0.08683283349019888
2215	13	0.02810541721849802
2215	14	0.08127709568204011
2215	15	0.11055485393958264
2215	16	0.05874312112638336
2215	17	0.2633971416692873
2215	18	0.18612869998702308
2215	19	0.09149661084973815
2215	20	0.04716845624616184
2215	21	0.027370107312648884
2215	22	0.21885594874263603
2215	23	0.09447579855525845
2215	24	0.10278673058067511
2215	25	-0.06627759304841874
2215	26	0.037404988318954224
2215	27	0.032724528436602895
2215	28	0.0483266234623585
2215	29	0.05512653771479137
2215	30	-0.019239693807158176
2215	31	0.09671185313917618
2215	32	-0.05312471062295998
2215	33	0.05666758924726878
2215	34	0.02482662396329971
2215	35	-0.1235582734331544
2215	36	-0.047884718525686765
2215	37	0.011741350721443666
2215	38	0.026533570788259908
2215	39	-0.02289834790467893
2215	40	-0.14299756349212345
2215	41	-0.18589379433978337
2215	42	0.01042033133237652
2215	43	-0.14385541338826613
2215	44	-0.111993122828288
2215	45	-0.019253822314407643
2215	46	-0.05124602709005188
2215	47	-0.06893319839035073
2215	48	-0.1868685897409712
2215	49	-0.2003323417219356
2215	50	-0.2942221679361374
2216	1	-0.07789111047307529
2216	2	-0.07315744919564438
2216	3	-0.0749158028329795
2216	4	0.04091936332756774
2216	5	0.021223326194238723
2216	6	-0.09546450298110898
2216	7	-0.08034172084684599
2216	8	-0.053629455648913193
2216	9	-0.012634300370990559
2216	10	-0.1385103825289704
2216	11	-0.37042971031173266
2216	12	-0.37656881184999236
2216	13	-0.25590206172369534
2216	14	-0.24506639786853412
2216	15	-0.37635124002335374
2216	16	-0.3254972873774733
2216	17	-0.2896152288614289
2216	18	-0.1615659475774791
2216	19	-0.34656200693445527
2216	20	-0.4047479464923711
2216	21	-0.5407387606727099
2216	22	-0.3443896425963686
2216	23	-0.38446991578464207
2216	24	-0.5298349563197249
2216	25	-0.5581223152533354
2216	26	-0.4591584709322589
2216	27	-0.3727807776703642
2216	28	-0.49939521840833834
2216	29	-0.6617563513432785
2216	30	-0.5760023725174364
2216	31	-0.6992023970836598
2216	32	-0.5944513863747147
2216	33	-0.6743511236865203
2216	34	-0.6067997784230315
2216	35	-0.7797906462396611
2216	36	-0.8705756408427661
2216	37	-0.8924550050383289
2216	38	-0.7876179329925286
2216	39	-0.7974202631966172
2216	40	-1.0881008950824906
2216	41	-0.927818778693748
2216	42	-0.8478509709870323
2216	43	-0.9702035126992996
2216	44	-1.1891350723654064
2216	45	-1.3299339373500487
2216	46	-1.3771122487138285
2216	47	-1.227976710634902
2216	48	-1.3245690757841615
2216	49	-1.2463359978829551
2216	50	-1.2942053365909558
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	202309.0
3	10069.0
4	18096.0
5	14170.0
6	11899.0
7	10039.0
8	8975.0
9	8495.0
10	8364.0
11	8703.0
12	8609.0
13	8923.0
14	9523.0
15	10036.0
16	10749.0
17	11682.0
18	12360.0
19	13906.0
20	16672.0
21	21363.0
22	25875.0
23	28865.0
24	31719.0
25	34739.0
26	38749.0
27	43563.0
28	50132.0
29	57951.0
30	67674.0
31	80127.0
32	97120.0
33	119985.0
34	151173.0
35	194577.0
36	253849.0
37	343656.0
38	527836.0
39	819604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.05424349322384	15.567844788619029	12.362786185441204	26.015125532715928
2	16.584158196724943	18.727303039736558	37.20647576163083	27.482063001907676
3	20.76525098667145	22.110942772170525	26.531885998797165	30.59192024236086
4	13.649305295290079	14.852238394586138	34.07098553576456	37.42747077435923
5	15.584135446018324	36.167971381551865	32.447756076300514	15.80013709612929
6	35.76880420034216	33.026429119226	16.376850923249368	14.827915757182467
7	31.479861974051854	29.964101337402894	19.438103786098125	19.117932902447134
8	28.512113543694916	32.83849503160792	18.502588838012453	20.146802586684704
9	27.339810659447178	15.926871385411918	17.17784407574802	39.55547387939289
10	16.139175079860333	28.26340534305509	31.311709083358817	24.285710493725755
11	36.72633921092557	21.567295009827447	21.679756166044932	20.026609613202048
12	25.527681176092337	23.438489061149603	27.62005893440229	23.413770828355766
13	29.306179945953563	20.240660591112956	24.23709996820451	26.216059494728967
14	23.445212037052876	19.54118660994963	24.96488612841324	32.04871522458425
15	24.997094732942543	27.20229566239204	21.76109915248788	26.039510452177534
16	25.028249737412384	26.093761107065248	23.360962181189436	25.51702697433293
17	24.79199431789356	26.30313259152899	24.565052213791613	24.339820876785833
18	24.625974183797535	26.05636004641513	25.301258836306445	24.016406933480894
19	25.360525619465125	25.441735514151848	25.404095185194254	23.793643681188772
20	25.881214581348218	24.96597154412784	24.13571852109705	25.01709535342689
21	26.11839019079679	24.823763007195815	24.321324572868324	24.73652222913907
22	25.58124920155037	24.288092722535474	24.484677290632124	25.64598078528203
23	25.43146905664095	25.1808615635477	24.55732203947776	24.83034734033359
24	25.41613070723044	24.960142319357086	24.845348658822974	24.778378314589506
25	25.238703867943848	24.83895123129949	24.50351778779741	25.418827112959256
26	25.376488495205514	24.83863725075699	24.916969398535972	24.86790485550153
27	24.980698044845443	24.728894930983525	25.085136594707325	25.205270429463706
28	24.141089377694055	25.030245409779567	25.405465537347325	25.423199675179053
29	24.825025240857677	25.523596587660276	24.45220995436878	25.19916821711327
30	25.12363406558846	25.11858898141816	24.811664942683713	24.946112010309673
31	25.351622045088796	24.835943141113674	24.782083410022985	25.03035140377455
32	25.216354612443492	25.054013071679513	24.50613986679381	25.223492449083185
33	25.06810598695574	25.259150801816006	24.285963736585273	25.386779474642985
34	24.984861958462925	25.125845569855464	24.642136108173954	25.24715636350766
35	25.457874269371533	25.02262382043471	24.864817511013996	24.65468439917976
36	25.496486859015103	25.067052161906823	24.355929068797156	25.080531910280918
37	25.30003666483883	24.52795126142679	25.184762175572956	24.98724989816142
38	25.621170726499066	24.873344377941496	24.412585426367198	25.092899469192236
39	25.322394014285887	24.909632930512256	24.546512900332385	25.22146015486947
40	25.293618296509496	24.710452156543724	25.01086928949357	24.985060257453206
41	24.92445472621732	25.173047782127604	24.848039420977845	25.054458070677228
42	24.956220003550765	25.3966741948403	24.743409997233705	24.90369580437523
43	25.078079057589463	24.301771207569672	25.295166854724883	25.324982880115986
44	25.681425554114444	24.643207449572476	24.68871151249843	24.98665548381465
45	24.95298088508581	24.75815160177567	24.85189494847507	25.436972564663446
46	24.748471069749886	25.38917688410797	24.588353591290552	25.273998454851586
47	25.583263444341824	24.73837336173716	24.546635714985026	25.13172747893599
48	24.9780465466289	25.08461441833227	24.969259267777876	24.96807976726096
49	25.893721487678604	24.28991032044672	24.54863139382956	25.267736798045114
50	25.075886472539622	24.75960191669738	24.612900847769996	25.551610762993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2047.0
1	1538.0
2	1029.0
3	979.0
4	929.0
5	952.5
6	976.0
7	985.5
8	995.0
9	1156.0
10	1317.0
11	1514.0
12	1711.0
13	2256.0
14	2801.0
15	3621.0
16	4441.0
17	4686.5
18	4932.0
19	4802.5
20	4673.0
21	4586.0
22	4499.0
23	5006.5
24	5514.0
25	6491.0
26	7468.0
27	9185.0
28	10902.0
29	14098.5
30	17295.0
31	22171.5
32	27048.0
33	35267.5
34	43487.0
35	58790.5
36	74094.0
37	85395.5
38	96697.0
39	116708.5
40	136720.0
41	161861.5
42	187003.0
43	198409.5
44	209816.0
45	228989.0
46	248162.0
47	274849.5
48	301537.0
49	308109.5
50	314682.0
51	306297.5
52	297913.0
53	290308.5
54	282704.0
55	282265.5
56	281827.0
57	258181.5
58	234536.0
59	209819.5
60	185103.0
61	161960.5
62	138818.0
63	117088.0
64	95358.0
65	79331.5
66	63305.0
67	51095.5
68	38886.0
69	31716.0
70	24546.0
71	19755.0
72	14964.0
73	12458.5
74	9953.0
75	7621.5
76	5290.0
77	4265.5
78	3241.0
79	2665.5
80	2090.0
81	1639.0
82	1188.0
83	950.5
84	713.0
85	554.0
86	395.0
87	309.0
88	223.0
89	177.0
90	131.0
91	101.5
92	72.0
93	51.5
94	31.0
95	26.0
96	21.0
97	20.0
98	19.0
99	26.5
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08549185527938738
2	0.09407052075742246
3	0.10229542683430147
4	0.10323878523738435
5	0.09648787666532238
6	0.05707318338651516
7	0.0518552322194629
8	0.059107299943162654
9	0.06055181749788334
10	0.04507484369730459
11	0.04811127855722765
12	0.05677838388555176
13	0.05064655426551294
14	0.051236153267439744
15	0.051354073067825114
16	0.05421362822717014
17	0.05436102797765184
18	0.05489166707938597
19	0.06352929245761373
20	0.06857036392408795
21	0.0785935469568437
22	0.08121726251541801
23	0.058193421490176096
24	0.07594035144817307
25	0.08224906076878993
26	0.08027390411233512
27	0.07614671109884745
28	0.0941000007075188
29	0.08224906076878993
30	0.07953690535992661
31	0.0544789477780372
32	0.05167835251888485
33	0.05400726857649576
34	0.04926099661098493
35	0.056129824983432275
36	0.05498010692967499
37	0.05798706183950172
38	0.0484355580082874
39	0.05297547032312383
40	0.054537907678229885
41	0.04286384744007905
42	0.038854574226976746
43	0.03956209302928892
44	0.03626033861849879
45	0.02835971199267954
46	0.025824436284394257
47	0.03077706790057946
48	0.025647556583816216
49	0.02473367813082966
50	0.026119235785357662
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3392136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.727492937742376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95503112073561	34.275056562216974
2	12.51782621898188	11.448176200487604
3	4.872205897153103	6.68381282259886
4	2.4907184409128176	4.5557723966698225
5	1.3783516031968066	3.1514281600453926
6	0.8460288784083019	2.3212067737525035
7	0.5613851846110502	1.7969515945258498
8	0.3828307998738455	1.4004714158065228
9	0.29364990002764296	1.2085086356714518
>10	1.4487747626675709	12.049883592595817
>50	0.13029484817598772	4.178091150878868
>100	0.10667235117736021	9.852303799298872
>500	0.01141377632370031	3.5639824350302844
>1k	0.00475024216870192	3.324048526501708
>5k	6.597558567641555E-5	0.1903059339193942
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	6308	0.18595952520771572	No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC	4301	0.1267932653643604	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.843985028902143E-5
2	0.0	0.0	0.0	0.0	8.843985028902143E-5
3	0.0	0.0	0.0	0.0	8.843985028902143E-5
4	0.0	0.0	0.0	0.0	8.843985028902143E-5
5	0.0	0.0	0.0	0.0	1.4739975048170238E-4
6	0.0	0.0	0.0	0.0	2.358396007707238E-4
7	0.0	0.0	0.0	0.0	2.358396007707238E-4
8	0.0	0.0	0.0	0.0	2.358396007707238E-4
9	0.0	0.0	0.0	2.9479950096340476E-5	2.358396007707238E-4
10	0.0	0.0	0.0	2.9479950096340476E-5	2.358396007707238E-4
11	0.0	0.0	0.0	2.9479950096340476E-5	2.358396007707238E-4
12	0.0	0.0	0.0	2.9479950096340476E-5	5.60119051830469E-4
13	0.0	0.0	0.0	2.9479950096340476E-5	6.485589021194905E-4
14	0.0	0.0	0.0	2.9479950096340476E-5	6.485589021194905E-4
15	0.0	0.0	0.0	2.9479950096340476E-5	8.254386026975333E-4
16	0.0	0.0	0.0	2.9479950096340476E-5	8.254386026975333E-4
17	0.0	0.0	0.0	2.9479950096340476E-5	8.549185527938738E-4
18	0.0	0.0	0.0	2.9479950096340476E-5	8.843985028902143E-4
19	0.0	0.0	0.0	2.9479950096340476E-5	8.843985028902143E-4
20	0.0	0.0	0.0	2.9479950096340476E-5	0.0010023183032755762
21	0.0	0.0	0.0	5.895990019268095E-5	0.0010907581535645977
22	0.0	0.0	0.0	2.0635965067438333E-4	0.001120238103660938
23	0.0	0.0	0.0	2.0635965067438333E-4	0.001120238103660938
24	0.0	0.0	0.0	2.9479950096340476E-4	0.0011497180537572787
25	0.0	0.0	0.0	2.9479950096340476E-4	0.0011497180537572787
26	0.0	0.0	0.0	4.4219925144510714E-4	0.0012086779539499596
27	0.0	0.0	0.0	6.78038852215831E-4	0.0012381579040463
28	0.0	0.0	0.0	0.0014150376046243428	0.0012381579040463
29	0.0	0.0	0.0	0.002977474959730388	0.0012381579040463
30	0.0	0.0	0.0	0.005306391017341286	0.0012381579040463
31	0.0	0.0	0.0	0.012057299589403256	0.0012381579040463
32	0.0	0.0	0.0	0.020577005167245654	0.0012381579040463
33	0.0	0.0	0.0	0.031720426303662355	0.0012381579040463
34	0.0	0.0	0.0	0.043571366242391225	0.0012676378541426406
35	0.0	0.0	0.0	0.058311341290561466	0.0012676378541426406
36	0.0	0.0	0.0	0.07815134770539861	0.0012676378541426406
37	0.0	0.0	0.0	0.10424110354065992	0.0012676378541426406
38	0.0	0.0	0.0	0.13472337194027598	0.001297117804238981
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	60	0.007402324	18.338684	24
TATCATA	1040	0.0	18.197617	2
TCATACT	1110	0.0	17.84357	4
TAGGCCG	150	9.582436E-9	17.604616	5
TATAGGC	1295	0.0	17.502659	5
GTCCTAG	565	0.0	16.74825	1
CTTATAG	1475	0.0	16.412294	3
TTATAGG	1400	0.0	16.348108	4
GTACATG	10965	0.0	15.71457	1
ATAGGCC	1485	0.0	15.554126	6
ATCGTAA	340	0.0	15.526609	10
TACATGG	10965	0.0	15.232898	2
TCTTATA	1645	0.0	14.983094	2
GTGTAGA	910	0.0	14.751555	1
ACGCGTC	90	0.004364254	14.665103	38
TAGGCCT	1600	0.0	14.573875	7
AATATCG	955	0.0	14.509401	33
ATATCGA	930	0.0	14.425802	34
GTCTATA	275	3.6379788E-12	14.404202	1
ATACTTG	1395	0.0	14.349936	6
>>END_MODULE