FastQCFastQC Report
Mon 6 Feb 2017
SRR2077149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3392136
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63710.18781676206378517No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC43830.12921062127226032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGC12950.016.1411115
CTTATAG14900.015.6528273
TTATAGG13850.015.251324
ACCGTCG1756.4050255E-815.0885818
TCTTATA16150.014.9862622
TACCGCG3250.014.89469817
ATAGGCC14200.014.8751896
CGATAAT900.004355659714.66945514
GTACATG104550.014.6475431
GTATCAA92350.014.3191481
TACATGG105700.014.258992
GTATTAA4200.014.1447111
CGATCGT1100.001018564614.00018325
TCTATAC3500.013.830183
CTCTTAT17750.013.759551
GTACTAG2256.719347E-913.6906821
GTATTAG2907.2759576E-1213.6569611
ACATGGG108950.013.4499353
CTTACGG1150.001421804313.39503510
CGTACAC2309.149517E-913.3928613
Produced by FastQC (version 0.11.5)