FastQCFastQC Report
Mon 6 Feb 2017
SRR2077148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183375
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50600.020.9182591
GGTATCA27050.020.1750851
TGCGATA1551.44355E-817.03034813
TATCAAC66900.015.7876972
ATCAACG67850.015.4695973
TCAACGC68250.015.2822114
CAACGCA68550.015.14975
AACGCAG71400.014.6647926
GTACATG122650.014.3533541
GTATAAA4600.014.3514041
TTACGTA1100.001017084814.0028314
TACATGG122900.013.9293662
GTCTAAT1901.7503044E-713.8982011
TTATTCG1606.335693E-613.75299628
TATTCGC1606.3387834E-613.75234929
GCACCGT4750.013.4298636
TACCGTA1658.7381195E-613.3314197
GTACTAA2155.457332E-813.3056431
GCGATAC2003.2794014E-713.19997214
TATTAGA2857.8216544E-1113.1252552
Produced by FastQC (version 0.11.5)