##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077148_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3183375
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.618300702870382	31.0	30.0	34.0	16.0	34.0
2	28.75281862802843	31.0	30.0	34.0	16.0	34.0
3	28.813509561393175	31.0	30.0	34.0	16.0	34.0
4	32.18391863980838	35.0	33.0	37.0	19.0	37.0
5	32.124616484077436	35.0	33.0	37.0	19.0	37.0
6	32.18249310872894	35.0	33.0	37.0	19.0	37.0
7	32.03209800918836	35.0	33.0	37.0	18.0	37.0
8	31.90576228059842	35.0	32.0	37.0	17.0	37.0
9	33.234400596850826	38.0	33.0	39.0	17.0	39.0
10	33.16946856716535	38.0	33.0	39.0	17.0	39.0
11	33.18137951073939	38.0	33.0	39.0	17.0	39.0
12	33.075054619703934	38.0	33.0	39.0	17.0	39.0
13	33.02194086464837	38.0	33.0	39.0	17.0	39.0
14	33.89466650960066	39.0	33.0	41.0	16.0	41.0
15	33.92821423803353	39.0	33.0	41.0	16.0	41.0
16	33.881869085483174	39.0	33.0	41.0	16.0	41.0
17	33.71234491695135	38.0	33.0	41.0	11.0	41.0
18	33.67974900852083	38.0	33.0	41.0	11.0	41.0
19	33.80556390623159	39.0	33.0	41.0	12.0	41.0
20	33.77299187183414	39.0	33.0	41.0	11.0	41.0
21	33.72479899477756	39.0	33.0	41.0	11.0	41.0
22	33.636564966427144	39.0	33.0	41.0	10.0	41.0
23	33.58629976047434	39.0	33.0	41.0	10.0	41.0
24	33.58364157537205	39.0	33.0	41.0	10.0	41.0
25	33.47727490477873	38.0	33.0	41.0	10.0	41.0
26	33.41328809832332	38.0	33.0	41.0	9.0	41.0
27	33.339611261632704	38.0	32.0	41.0	9.0	41.0
28	33.22401507833667	38.0	32.0	40.0	8.0	41.0
29	33.19521419876703	38.0	32.0	40.0	8.0	41.0
30	33.03673585424274	38.0	32.0	40.0	8.0	41.0
31	33.02167102524836	38.0	32.0	40.0	2.0	41.0
32	32.890329603015665	38.0	32.0	40.0	2.0	41.0
33	32.83249758511014	38.0	32.0	40.0	2.0	41.0
34	32.7401641339773	38.0	32.0	40.0	2.0	41.0
35	32.66524678996348	38.0	32.0	40.0	2.0	41.0
36	32.56837350296462	38.0	31.0	40.0	2.0	41.0
37	32.468880983233205	38.0	31.0	40.0	2.0	41.0
38	32.36956877527781	38.0	31.0	40.0	2.0	41.0
39	32.255842619861	38.0	31.0	40.0	2.0	41.0
40	32.17856583029018	38.0	31.0	40.0	2.0	41.0
41	32.05250371068441	38.0	31.0	40.0	2.0	41.0
42	31.89628837318883	38.0	31.0	40.0	2.0	41.0
43	31.803110849334434	38.0	30.0	40.0	2.0	41.0
44	31.64387953037264	38.0	30.0	40.0	2.0	41.0
45	31.49031169749087	38.0	30.0	40.0	2.0	41.0
46	31.332007696234342	37.0	30.0	40.0	2.0	41.0
47	31.15325527152786	37.0	30.0	40.0	2.0	41.0
48	30.97195774924412	37.0	30.0	40.0	2.0	41.0
49	30.771496289315586	37.0	30.0	40.0	2.0	41.0
50	30.549969136529626	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7830709920105186
1101	2	1.1037996870529128
1101	3	1.2093944938565286
1101	4	1.2800668434554154
1101	5	1.2247173937199776
1101	6	1.236953149555326
1101	7	1.3365048019202703
1101	8	1.3658290315864363
1101	9	1.5425068234636257
1101	10	1.6696394165579633
1101	11	1.6928475447660958
1101	12	1.632731014600175
1101	13	1.498583209526629
1101	14	1.9500962870797878
1101	15	1.9105608903917073
1101	16	1.8561189164570564
1101	17	1.9433676927487298
1101	18	1.977460119510404
1101	19	1.9048799253633817
1101	20	2.0143494378997673
1101	21	2.050182135307381
1101	22	1.8940397656908843
1101	23	1.8362163562661138
1101	24	1.799178681125106
1101	25	1.826164575848587
1101	26	1.9863835405573909
1101	27	1.9643109363554103
1101	28	2.009053575443005
1101	29	1.8412888957125162
1101	30	2.0800690845490024
1101	31	1.970570334287423
1101	32	1.9578163792485768
1101	33	1.9955074501901677
1101	34	2.0986629084857285
1101	35	2.0344090859056365
1101	36	2.1863853660455206
1101	37	2.1459892077285687
1101	38	2.0078342918319905
1101	39	2.161121540737625
1101	40	2.158971821003071
1101	41	2.1515484502845723
1101	42	2.136753580499679
1101	43	2.1418687910648373
1101	44	2.055228020233322
1101	45	1.9854842356340718
1101	46	2.0492572280433095
1101	47	1.9781759333860407
1101	48	1.8906782130634205
1101	49	2.068273248836313
1101	50	2.19052939879305
1102	1	0.842041866473533
1102	2	0.9668502842910485
1102	3	0.9374323655169974
1102	4	1.0676224445229536
1102	5	1.2045513176424265
1102	6	1.1434789281836046
1102	7	1.2630457559510333
1102	8	1.2571454159721256
1102	9	1.2922782324080089
1102	10	1.46414540885079
1102	11	1.2349388062516482
1102	12	1.4336055558358112
1102	13	1.381857341723304
1102	14	1.6584718434460228
1102	15	1.4881769583979931
1102	16	1.5635141162226702
1102	17	1.8381904911242373
1102	18	1.7876176834245214
1102	19	1.7693371273861018
1102	20	1.680391168123208
1102	21	1.705911276418
1102	22	1.7269261571096024
1102	23	1.603654664414968
1102	24	1.6439802524263882
1102	25	1.704506346677256
1102	26	1.730288027967994
1102	27	1.6816490825811528
1102	28	1.864433554294159
1102	29	1.7497438136024996
1102	30	1.750303890835056
1102	31	1.863835484978317
1102	32	2.073364339991649
1102	33	1.943332226203644
1102	34	1.95094579676163
1102	35	1.958862746527032
1102	36	2.0475063973470995
1102	37	1.9661062161973533
1102	38	1.8644285873313535
1102	39	2.0726574777831672
1102	40	2.0779196692462065
1102	41	2.024553229754112
1102	42	2.0737593043429072
1102	43	1.8863081865234506
1102	44	1.8242923862691498
1102	45	1.830644782716579
1102	46	1.841308133198467
1102	47	1.8800616229246145
1102	48	1.8704657716512152
1102	49	2.004954273111977
1102	50	2.043295994133903
1103	1	0.3917597730879905
1103	2	0.485904218862224
1103	3	0.36221132219250407
1103	4	0.4960265117201246
1103	5	0.578061906670257
1103	6	0.49413574263352444
1103	7	0.5479748115697447
1103	8	0.586823113753276
1103	9	0.6987674328627236
1103	10	0.7364781527352591
1103	11	0.6918290424522269
1103	12	0.5936767107345489
1103	13	0.642660044767382
1103	14	0.7583886890525235
1103	15	0.6764374526929657
1103	16	0.624741011932322
1103	17	0.8044161237291831
1103	18	0.6327126267953318
1103	19	0.7886857863899337
1103	20	0.6661328211062383
1103	21	0.5046492242938783
1103	22	0.5437917627683078
1103	23	0.7326050243998097
1103	24	0.623705316634215
1103	25	0.7172194955972984
1103	26	0.7628677088235349
1103	27	0.6774050507509131
1103	28	0.704924480991977
1103	29	0.7316645748336015
1103	30	0.8732745025987754
1103	31	0.7271201887802619
1103	32	0.8960707523532321
1103	33	0.8485941099138046
1103	34	0.8548719472395021
1103	35	0.7466401032699039
1103	36	0.8454296426841381
1103	37	0.8542667147026748
1103	38	0.8515470244497934
1103	39	0.9537951140932179
1103	40	0.9327647284016081
1103	41	0.8938197869517026
1103	42	0.8242171798007831
1103	43	0.8345640494344906
1103	44	0.6184852623585755
1103	45	0.5963483320408152
1103	46	0.6794770524018645
1103	47	0.6443852303516664
1103	48	0.6009255417661592
1103	49	0.6527406821052857
1103	50	0.7118941584217211
1104	1	0.3772393389844382
1104	2	0.3758482514979704
1104	3	0.4141039526363457
1104	4	0.399183420860048
1104	5	0.4533390141913216
1104	6	0.33443324749613623
1104	7	0.3695359490083945
1104	8	0.48353163340311767
1104	9	0.5194560297350606
1104	10	0.33614968981029847
1104	11	0.36647194972508856
1104	12	0.47680083485198566
1104	13	0.39490002043762473
1104	14	0.5865321744914311
1104	15	0.5155989559692458
1104	16	0.4737160204544253
1104	17	0.5267930991298329
1104	18	0.34569774598219993
1104	19	0.5116601443359841
1104	20	0.4066214883833794
1104	21	0.42908130705072267
1104	22	0.4493231630886996
1104	23	0.4947894003258213
1104	24	0.44116581976120983
1104	25	0.5362875008464698
1104	26	0.45270557725121563
1104	27	0.6001892488825149
1104	28	0.4483156754897095
1104	29	0.5113019900860465
1104	30	0.4435019034360579
1104	31	0.510204041794637
1104	32	0.5661117252763432
1104	33	0.603270960769251
1104	34	0.47844184963081915
1104	35	0.5394082375202842
1104	36	0.6474782331398359
1104	37	0.5921859407845389
1104	38	0.6063606188156285
1104	39	0.6231520909643464
1104	40	0.6558101038530566
1104	41	0.6994083911764406
1104	42	0.6749392189556573
1104	43	0.7251661776202525
1104	44	0.7889247688119809
1104	45	0.8502102918343262
1104	46	0.6264679153731691
1104	47	0.5889308631371364
1104	48	0.7102150770721636
1104	49	0.7097181659355734
1104	50	0.844925542032108
1105	1	-0.007759793854443586
1105	2	-0.021824900173609052
1105	3	-0.1035932755086435
1105	4	0.024101754686476795
1105	5	-0.0018385498000696998
1105	6	0.09170403640871427
1105	7	-0.060094028241380215
1105	8	-0.05515538264446107
1105	9	-0.008498184157879507
1105	10	-0.06627060788101602
1105	11	-0.02004971400003086
1105	12	-0.017719727068417512
1105	13	-0.0015774690799261748
1105	14	0.09200018644805397
1105	15	-0.013530924362520125
1105	16	-0.07652280591951666
1105	17	0.026142422234066487
1105	18	-0.12702299753194524
1105	19	-0.2637789962563346
1105	20	-0.08866176724551877
1105	21	-0.032142716372831615
1105	22	-0.07973244744373886
1105	23	-0.11802295621002656
1105	24	-0.03831265934020678
1105	25	-0.09520411829637254
1105	26	0.04145288650127554
1105	27	0.0737061483224295
1105	28	0.05224340526190474
1105	29	-0.003873329488328636
1105	30	0.03739668108574534
1105	31	-0.13460186714324607
1105	32	0.11012453885710727
1105	33	0.03458506693016972
1105	34	0.07561714880139903
1105	35	0.0345886580424164
1105	36	0.06202123923036851
1105	37	0.036485427629195044
1105	38	0.11848026664619482
1105	39	0.14340754642797293
1105	40	0.1679183255606489
1105	41	0.08763213914880907
1105	42	0.05543727549456179
1105	43	0.09687142445510943
1105	44	0.10413885558930147
1105	45	0.22431216796291054
1105	46	0.16417146869337884
1105	47	0.06897656762902926
1105	48	0.18157080696677852
1105	49	0.18960961776732432
1105	50	0.2553188251041014
1106	1	0.15147952200180015
1106	2	0.1110612889876208
1106	3	0.10615629436327367
1106	4	0.10232343460532434
1106	5	0.13670160326241643
1106	6	0.036991049147459876
1106	7	0.15968906463793076
1106	8	0.25691624774448485
1106	9	0.22474913880359537
1106	10	0.2810454569112295
1106	11	0.16470436981571623
1106	12	0.21360695166591626
1106	13	0.3146765520802717
1106	14	0.30561672897745495
1106	15	0.2598084153668623
1106	16	0.17476441353681338
1106	17	0.29185110226907796
1106	18	0.04654426206052875
1106	19	0.3175718574598676
1106	20	0.1725056383298167
1106	21	0.26169203212229064
1106	22	0.31882861519443395
1106	23	0.23361328668863024
1106	24	0.21575610078415508
1106	25	0.26302974515477473
1106	26	0.24705145740586687
1106	27	0.40781354451315366
1106	28	0.3117742295071295
1106	29	0.22189978057780735
1106	30	0.36194618766183595
1106	31	0.23088441769346701
1106	32	0.3523789679644054
1106	33	0.29918160633136637
1106	34	0.34675994034866875
1106	35	0.3018352437721319
1106	36	0.35476254314272637
1106	37	0.35174135040828247
1106	38	0.35881368565451766
1106	39	0.390935359547953
1106	40	0.4986558225765094
1106	41	0.3392934991493206
1106	42	0.34225866326475085
1106	43	0.22814525300208288
1106	44	0.32906003307464715
1106	45	0.3663324939730117
1106	46	0.3819468398474939
1106	47	0.3994810042824355
1106	48	0.4735317428303567
1106	49	0.2996139624954317
1106	50	0.3796513696787258
1107	1	-0.10340793574849627
1107	2	-0.13243248813174802
1107	3	0.01647540056918828
1107	4	-0.056433049772905974
1107	5	-0.10574417769694122
1107	6	-0.2065110792653897
1107	7	-0.049199614516389545
1107	8	-0.12702382103953624
1107	9	-0.1544989253191673
1107	10	-0.1764138199571974
1107	11	-0.24694700474518783
1107	12	-0.34080927432110286
1107	13	-0.2411640982382366
1107	14	-0.30186099002683164
1107	15	-0.23376342264197092
1107	16	-0.26264074549114014
1107	17	-0.04947516044831701
1107	18	-0.13475491620866364
1107	19	-0.17339404876742748
1107	20	-0.11531082372529511
1107	21	-0.2585880082644181
1107	22	-0.4940196273615527
1107	23	-0.5116082235301036
1107	24	-0.4900940204117106
1107	25	-0.4267040936518427
1107	26	-0.19299121515100381
1107	27	-0.20716093736201913
1107	28	-0.16518958444763854
1107	29	-0.15393293272972386
1107	30	-0.3118270893806212
1107	31	-0.31342677965518106
1107	32	-0.2517933163754762
1107	33	-0.30244353349809217
1107	34	-0.27291664797071036
1107	35	-0.26095893418743543
1107	36	-0.4141772240465542
1107	37	-0.4185011091577806
1107	38	-0.37595156750892755
1107	39	-0.31710670267158747
1107	40	-0.2216755509136874
1107	41	-0.27967392212660513
1107	42	-0.3417023165746471
1107	43	-0.3462549983098562
1107	44	-0.21203927139024614
1107	45	-0.1307863462460297
1107	46	-0.1856596889238773
1107	47	-0.06113382159017533
1107	48	-0.22971591109437384
1107	49	-0.27960199473310965
1107	50	-0.18209309809990515
1108	1	-0.016774464383384924
1108	2	-0.10558468397255538
1108	3	-0.04207504927790495
1108	4	-0.01863819449980042
1108	5	-0.08356711534635508
1108	6	-0.14385129139012776
1108	7	-0.26191415198519863
1108	8	-0.05097995483644624
1108	9	-0.21451959208992832
1108	10	-0.19953496689272043
1108	11	-0.09672779847019797
1108	12	-0.16823406764981286
1108	13	-0.06695063346572994
1108	14	-0.2829049558789123
1108	15	-0.16640909190395803
1108	16	-0.18948490780228866
1108	17	-0.3092752086526218
1108	18	-0.15084729641587558
1108	19	-0.14707811214074695
1108	20	-0.24464417483773104
1108	21	-0.16367822214556327
1108	22	-0.25818437309676057
1108	23	-0.34369967234184884
1108	24	-0.1726835923096104
1108	25	-0.17163622551943547
1108	26	-0.2847041325709512
1108	27	-0.3421327725422998
1108	28	-0.44431938636408574
1108	29	-0.3605106694817479
1108	30	-0.4277889795915186
1108	31	-0.2981275050568257
1108	32	-0.2214051771003085
1108	33	-0.2762587177390472
1108	34	-0.33069155623987
1108	35	-0.17235771783886378
1108	36	-0.5015830826330188
1108	37	-0.36298651244441515
1108	38	-0.2929609478484849
1108	39	-0.2097383551199954
1108	40	-0.3206348139756976
1108	41	-0.17884864208068052
1108	42	-0.09042956954156978
1108	43	-0.12051992210848894
1108	44	-0.2152717699804647
1108	45	-0.14027171153700735
1108	46	-0.13058777747986028
1108	47	-0.15733983319161382
1108	48	-0.2754621823939871
1108	49	-0.2944510566202929
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2210	33	-0.08729375324122657
2210	34	-0.15566876103402905
2210	35	-0.3211359074873741
2210	36	-0.385080875397648
2210	37	-0.23250261154533547
2210	38	-0.20816426163944612
2210	39	-0.11452393223288482
2210	40	-0.3405001436617958
2210	41	-0.3314397711207775
2210	42	-0.30650085544480987
2210	43	-0.39536141246897927
2210	44	-0.4100677417917673
2210	45	-0.2980910483814583
2210	46	-0.30420925405120514
2210	47	-0.31079817344699734
2210	48	-0.2460321661065663
2210	49	-0.20546077116133077
2210	50	-0.09320617386432772
2211	1	-0.16984034911863333
2211	2	-0.24160200915281393
2211	3	-0.18914695735237785
2211	4	-0.18519522684446343
2211	5	-0.3929197891035514
2211	6	-0.15733747482504157
2211	7	-0.1957270245755396
2211	8	-0.10568220048112309
2211	9	-0.07879124523820025
2211	10	-0.23250764766735443
2211	11	-0.17930758067602426
2211	12	-0.37229395967585077
2211	13	-0.24208787176659996
2211	14	-0.41421582114720223
2211	15	-0.2230161018675787
2211	16	-0.2886224300116851
2211	17	-0.23763636143111455
2211	18	-0.2986488325215859
2211	19	-0.19385246548308288
2211	20	-0.44939281967089784
2211	21	-0.3897233868389236
2211	22	-0.32856865723849893
2211	23	-0.4860253014875937
2211	24	-0.3098206028921737
2211	25	-0.39714684367745434
2211	26	-0.3789316637137574
2211	27	-0.3140220254002415
2211	28	-0.2383117423803114
2211	29	-0.20697920855054264
2211	30	-0.6714138705117065
2211	31	-0.5656748585286522
2211	32	-0.19872291320859148
2211	33	-0.28657839156532106
2211	34	-0.2774847945694674
2211	35	-0.22715977507727558
2211	36	-0.2574799410287838
2211	37	-0.24452081837571882
2211	38	-0.38746499407318424
2211	39	-0.2049899637765833
2211	40	-0.3981975056905327
2211	41	-0.33003870257812196
2211	42	-0.31759938381173214
2211	43	-0.32616085090342395
2211	44	-0.40897649961356564
2211	45	-0.3634795864499516
2211	46	-0.469377583558078
2211	47	-0.4061563000555388
2211	48	-0.42171014312166477
2211	49	-0.397722046259112
2211	50	-0.2704148406561906
2212	1	-0.1681646342439187
2212	2	-0.3137625335480472
2212	3	-0.28315531930426374
2212	4	-0.25810681405302205
2212	5	-0.22259412410155477
2212	6	-0.2761301731773216
2212	7	-0.2218361099527293
2212	8	-0.07372452511679128
2212	9	-0.23440821982588744
2212	10	-0.2774622130487998
2212	11	-0.28385480889233605
2212	12	-0.372631354050732
2212	13	-0.32204897094213436
2212	14	-1.0836027891395545
2212	15	-0.5462249738141622
2212	16	-0.5488215610765153
2212	17	-0.30098938820162857
2212	18	-0.2726409549363069
2212	19	-0.31370521551025377
2212	20	-0.25655525933417067
2212	21	-0.32492925369260206
2212	22	-0.3084739658817668
2212	23	-0.39869286639972046
2212	24	-0.393170269395668
2212	25	-0.17129376561921106
2212	26	-0.39502591194448655
2212	27	-0.46318126954847116
2212	28	-0.44094152480354865
2212	29	-0.37388211440299557
2212	30	-0.4030306902367471
2212	31	-0.23656290009159875
2212	32	-0.13432351149182864
2212	33	-0.21938178322569257
2212	34	-0.08537052276636814
2212	35	-0.10035135648269033
2212	36	-0.23984064328460875
2212	37	-0.18073834232603758
2212	38	-0.348868739834586
2212	39	-0.37382612782452185
2212	40	-0.2420147353597315
2212	41	-0.2276717553175871
2212	42	-0.08690484902197682
2212	43	-0.043879063371825566
2212	44	-0.04913345795961632
2212	45	-0.07442192672969483
2212	46	-0.07110240840915694
2212	47	-0.10656440311747417
2212	48	-0.149041728148255
2212	49	-0.01348908401750748
2212	50	-0.05189753901583671
2213	1	-0.13470021120557618
2213	2	-0.353559977610967
2213	3	-0.2617267087920041
2213	4	-0.48015504036097667
2213	5	-0.28219546464408296
2213	6	-0.17867963539175236
2213	7	-0.4850979377698721
2213	8	-0.3255332080858082
2213	9	-0.2768340521090735
2213	10	-0.4751927975039081
2213	11	-0.4744538875113591
2213	12	-0.44804350058836206
2213	13	-0.23911335424739377
2213	14	-0.25012491129015757
2213	15	-0.5243996287663037
2213	16	-0.26581371070636095
2213	17	-0.5291313882291249
2213	18	-0.3133589802582861
2213	19	-0.3389557499847342
2213	20	-0.3805313468098035
2213	21	-0.49926845972125733
2213	22	-0.3637187676807514
2213	23	-0.3178786623282548
2213	24	-0.3620339565863233
2213	25	-0.4432092186170209
2213	26	-0.48572228189539146
2213	27	-0.5458136970180547
2213	28	-0.612729192171436
2213	29	-0.5626525523171111
2213	30	-0.7775786422573674
2213	31	-0.6088546809277418
2213	32	-0.5998063091160333
2213	33	-0.6599551868663767
2213	34	-0.6242634850443523
2213	35	-0.7321115923108792
2213	36	-0.8377912468710811
2213	37	-0.7073453364146189
2213	38	-0.6651767565168676
2213	39	-0.8316425242304746
2213	40	-0.6742621153193866
2213	41	-0.4944235913591406
2213	42	-0.49100021150356454
2213	43	-0.5996563403706077
2213	44	-0.6257319147671474
2213	45	-0.7003872287502162
2213	46	-0.5673905852078995
2213	47	-0.5388624315316761
2213	48	-0.6901342854630848
2213	49	-0.7302731681124612
2213	50	-0.6138536819438087
2214	1	-0.21667442492974232
2214	2	-0.43591371071671503
2214	3	-0.3857724075492719
2214	4	-0.34094147539830644
2214	5	-0.23305822578429058
2214	6	-0.21555022364332643
2214	7	-0.04586755296227096
2214	8	-0.211545880420001
2214	9	-0.37574405001871014
2214	10	-0.2638094310154031
2214	11	-0.41360969049629404
2214	12	-0.4139255526371244
2214	13	-0.26295915295468575
2214	14	-0.38115015351669257
2214	15	-0.1960105684488127
2214	16	-0.2642773597682506
2214	17	-0.07668306253047774
2214	18	-0.19311214384269704
2214	19	-0.31441611536990877
2214	20	-0.22920738967479792
2214	21	-0.2797444959413724
2214	22	-0.2772746282546166
2214	23	-0.35841244668261396
2214	24	-0.45929142203642215
2214	25	-0.31376199624482837
2214	26	-0.5427156688584134
2214	27	-0.391852485103243
2214	28	-0.3203915313619561
2214	29	-0.23669461715726214
2214	30	-0.1874568402774699
2214	31	-0.2314574031123584
2214	32	-0.4269559614366898
2214	33	-0.3648038359490329
2214	34	-0.40415788687391085
2214	35	-0.4729610775405604
2214	36	-0.3178293441659221
2214	37	-0.29673374470355185
2214	38	-0.2574821273771448
2214	39	-0.19468361448867455
2214	40	-0.2528052537224852
2214	41	-0.07735065275918629
2214	42	-0.12372834189820026
2214	43	-0.20482923868599556
2214	44	-0.07539610621712711
2214	45	-0.0017702629948672666
2214	46	-0.08603586782974659
2214	47	-0.1320374506703068
2214	48	-0.2098402565772517
2214	49	-0.18896169088708703
2214	50	-0.12766447045537177
2215	1	0.02052528989782232
2215	2	-0.08186429504113946
2215	3	-0.08324411087564343
2215	4	0.04663213391372523
2215	5	0.08075206243712785
2215	6	-0.05593287333068986
2215	7	0.026005296045923387
2215	8	-0.017123868546828902
2215	9	-0.0030570936763325562
2215	10	-0.04078239804810835
2215	11	-0.115107643137776
2215	12	-0.061799404220927556
2215	13	-0.06061232177133036
2215	14	-0.08665979524671741
2215	15	-0.03281993053582255
2215	16	-0.11402636052937254
2215	17	-0.04544087240199701
2215	18	0.06122849369761951
2215	19	-0.01921594605527588
2215	20	-0.055090549750104856
2215	21	0.017198879164212144
2215	22	0.061592804522632605
2215	23	-0.05082637797671907
2215	24	-0.1743042809698423
2215	25	-0.11704328196830005
2215	26	-0.21323753684534807
2215	27	-0.2048430499030971
2215	28	-0.09938084710237405
2215	29	-0.09147885626668995
2215	30	-0.18632887339297355
2215	31	-0.06521969112021964
2215	32	-0.1566373126118492
2215	33	-0.231272552868937
2215	34	-0.3852585741339354
2215	35	-0.3315767015996727
2215	36	-0.2049947297926593
2215	37	-0.28982141581438015
2215	38	-0.25562711984655806
2215	39	-0.35463903939508157
2215	40	-0.32832845553017265
2215	41	-0.49440469945348653
2215	42	-0.4254777704526411
2215	43	-0.4597668801823609
2215	44	-0.48013643064324185
2215	45	-0.4074802985096859
2215	46	-0.4579215610378
2215	47	-0.5070008984348888
2215	48	-0.6304791003843278
2215	49	-0.6105862966992852
2215	50	-0.5880226069613101
2216	1	-0.0673792863599445
2216	2	-0.053218809332548744
2216	3	-0.07326152195414082
2216	4	0.01837917594319549
2216	5	-0.02530846975290757
2216	6	-0.056421771432731305
2216	7	-0.04561478834931876
2216	8	-0.1680042000779629
2216	9	-0.04483823722031133
2216	10	-0.06208366486643513
2216	11	-0.1718844494815599
2216	12	-0.14961568842808504
2216	13	-0.2024545864748788
2216	14	-0.1578131880048801
2216	15	-0.2954928736130711
2216	16	-0.2638159418182866
2216	17	-0.3217847416471997
2216	18	-0.2644474289249459
2216	19	-0.3708780234451581
2216	20	-0.4267238548211978
2216	21	-0.5387310743854883
2216	22	-0.36511488241959
2216	23	-0.4033165002813206
2216	24	-0.6134885136679671
2216	25	-0.6831956827309398
2216	26	-0.5131029991197167
2216	27	-0.5618139314979729
2216	28	-0.6990271450907173
2216	29	-0.7842538369828347
2216	30	-0.6245330678881587
2216	31	-0.7122657597485187
2216	32	-0.6956370430381753
2216	33	-0.8687569589661095
2216	34	-0.7875298613348818
2216	35	-0.872699035556078
2216	36	-0.8661149996866158
2216	37	-0.8496698139862602
2216	38	-0.9664105472044611
2216	39	-0.9401027372519266
2216	40	-1.1477722796684944
2216	41	-1.0168219301044594
2216	42	-0.9082235784930575
2216	43	-1.096409148800518
2216	44	-1.2225551737537685
2216	45	-1.467902167963075
2216	46	-1.476171396224192
2216	47	-1.4214046201530053
2216	48	-1.4716240500594608
2216	49	-1.5195329931762345
2216	50	-1.618008914753947
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	187609.0
3	9564.0
4	17386.0
5	13956.0
6	11633.0
7	10177.0
8	9094.0
9	8244.0
10	8124.0
11	8078.0
12	8402.0
13	8750.0
14	9251.0
15	9672.0
16	10489.0
17	11249.0
18	12300.0
19	13520.0
20	16114.0
21	20460.0
22	24642.0
23	27606.0
24	30075.0
25	33336.0
26	37053.0
27	41759.0
28	47485.0
29	54552.0
30	63497.0
31	76300.0
32	91431.0
33	113819.0
34	144869.0
35	185905.0
36	239942.0
37	322245.0
38	488364.0
39	756423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83230106580313	16.175873235451316	12.555097934416953	25.43672776432861
2	16.976219897559734	18.44949833196558	37.95021994157949	26.624061828895197
3	20.709374271857943	22.590083652418745	26.547717537009664	30.152824538713652
4	14.06325369967291	14.746272655406386	34.10840257019646	37.08207107472424
5	16.078969850202164	36.007638145828466	31.816342399727326	16.097049604242045
6	35.431481239344826	33.097651712120374	16.470515019085024	15.000352029449779
7	31.60480556930596	29.9675804791426	19.15599242549246	19.271621526058976
8	28.26079576957458	33.23903890085381	18.47913138483318	20.021033944738434
9	27.7288903389578	15.720622431602113	17.54412599506264	39.006361234377444
10	16.52352118709186	27.799146501167048	31.004748329724976	24.67258398201612
11	37.471632256618825	21.81380074805044	21.043624961539926	19.670942033790812
12	25.433572521915142	23.285684201924195	27.39759051291964	23.88315276324102
13	29.57087708127146	20.222775443471853	24.13424733222305	26.072100143033637
14	23.761570280012485	19.84309897286117	24.527877888621706	31.86745285850464
15	25.65109834590455	27.015006573449302	21.731543662129422	25.60235141851673
16	25.770426448942953	26.124771226977728	23.223443706985723	24.8813586170936
17	24.74570656377695	26.19234179401375	24.31445356023383	24.747498081975472
18	24.747362995184872	25.80515080273067	24.971178385989614	24.476307816094845
19	25.891083444814374	25.355157213409914	24.820425421700076	23.93333392007564
20	25.867420425032705	24.806598990196825	24.678251761748818	24.64772882302165
21	26.203561097849555	24.7167051309499	24.567595122471094	24.512138648729454
22	26.015713691522834	24.279479991310343	24.42532412514741	25.279482192019408
23	25.43622783579132	24.476597317585806	24.764382749595555	25.322792097027317
24	25.21237446313006	24.744996601631343	24.5871500796306	25.455478855608
25	25.504931257918777	24.523647087303857	24.60743288490378	25.36398876987358
26	25.162459640529555	24.857315321036598	24.91371640378648	25.066508634647366
27	25.286538603608633	24.830830701810918	24.546107573597485	25.336523120982967
28	25.23726139834163	24.94559566186521	24.50246875111033	25.31467418868283
29	25.245722589312297	25.07912514175245	24.61781464013977	25.057337628795484
30	25.416731901376437	25.00322243747057	24.608135884372025	24.971909776780972
31	25.62175481826982	24.516601501112632	24.45867540010812	25.402968280509423
32	25.22205596115085	25.045863226539666	24.391850630299178	25.3402301820103
33	25.28866193559783	24.67332335322754	24.45104965547891	25.586965055695714
34	25.665941293828798	24.80193675804856	24.476493268615492	25.055628679507148
35	25.591968538981163	24.811257774419566	24.533754841921706	25.063018844677565
36	25.41942390463241	24.992755276704425	24.304239404631467	25.28358141403169
37	25.449943863098884	24.598209402585187	24.758353585783848	25.19349314853208
38	25.63776676739439	24.765474119575277	24.485825048046024	25.11093406498431
39	25.84796023753384	24.799776471275994	24.213547877325723	25.13871541386444
40	25.797958272829565	24.567358766917483	24.604289638611775	25.030393321641174
41	25.258789932561726	24.91162063355333	24.553923434194807	25.275665999690133
42	25.714095366071486	24.793802154400595	24.478888093476776	25.013214386051143
43	25.392692204230116	24.390943693167106	24.591406526936623	25.62495757566616
44	25.40831452277646	24.62388051915566	24.40007076938451	25.56773418868337
45	25.377757136913864	24.73605390533385	24.42070116651605	25.465487791236235
46	25.505946171500106	24.47454118581566	24.345839439544115	25.673673203140122
47	25.33443271187442	24.660979561363046	24.486960172247144	25.517627554515393
48	25.366789555660922	24.835674068599772	24.488813519486122	25.308722856253183
49	25.535466169623515	24.610988533108944	24.38192721945959	25.47161807780795
50	25.56106270101919	24.659924864856034	24.51277787623188	25.266234557892897
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2060.0
1	1535.5
2	1011.0
3	962.0
4	913.0
5	916.0
6	919.0
7	908.5
8	898.0
9	994.0
10	1090.0
11	1259.5
12	1429.0
13	1935.0
14	2441.0
15	3201.0
16	3961.0
17	4147.0
18	4333.0
19	4320.5
20	4308.0
21	4571.5
22	4835.0
23	5797.5
24	6760.0
25	8505.5
26	10251.0
27	13149.5
28	16048.0
29	20362.0
30	24676.0
31	31194.0
32	37712.0
33	47375.0
34	57038.0
35	69152.5
36	81267.0
37	92537.5
38	103808.0
39	118087.0
40	132366.0
41	146581.5
42	160797.0
43	171115.5
44	181434.0
45	195791.5
46	210149.0
47	224641.0
48	239133.0
49	245782.5
50	252432.0
51	255212.5
52	257993.0
53	254842.5
54	251692.0
55	247344.0
56	242996.0
57	232757.5
58	222519.0
59	205758.0
60	188997.0
61	168293.5
62	147590.0
63	127696.5
64	107803.0
65	91657.5
66	75512.0
67	65196.5
68	54881.0
69	45316.0
70	35751.0
71	28877.5
72	22004.0
73	18433.0
74	14862.0
75	11348.5
76	7835.0
77	6165.5
78	4496.0
79	3623.5
80	2751.0
81	2131.0
82	1511.0
83	1222.5
84	934.0
85	739.5
86	545.0
87	409.0
88	273.0
89	216.0
90	159.0
91	115.0
92	71.0
93	55.5
94	40.0
95	34.5
96	29.0
97	25.5
98	22.0
99	31.0
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08403031373934897
2	0.09376840617269408
3	0.10027093886205679
4	0.10143322731377862
5	0.09493069462441592
6	0.05726626614834884
7	0.05183178230651431
8	0.05814583578748969
9	0.06021910707975027
10	0.044418266776612876
11	0.04724545490242274
12	0.05607256449522912
13	0.050449601445007264
14	0.05101503907016924
15	0.05139199748694389
16	0.05428201201554953
17	0.053905053598774885
18	0.05450190442533475
19	0.06178976714964464
20	0.0678839282208348
21	0.07859582989751443
22	0.08110888600934543
23	0.05773746416931715
24	0.07655397180665174
25	0.08308791769741233
26	0.0812031256135391
27	0.07507755134095102
28	0.09493069462441592
29	0.08337063650999332
30	0.08019790316880669
31	0.05450190442533475
32	0.051266344681352334
33	0.05377940079318333
34	0.049098833784898106
35	0.05613539089802489
36	0.05494168924490517
37	0.058648447009855886
38	0.04806219813876782
39	0.052805591549848825
40	0.055130168453292494
41	0.042533474692739624
42	0.03885813012918679
43	0.03892095653198257
44	0.03794714728864806
45	0.028491773667883927
46	0.025853064750461383
47	0.030502218557348724
48	0.026010130757450817
49	0.025790238347665605
50	0.02648132877841913
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3183375.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.641522863291094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15510891366935	42.93027756639648
2	11.862515021575462	13.200968015782502
3	4.407649345555263	7.357449655022497
4	2.0988797372134194	4.671394595418355
5	1.1776694337635583	3.2763660362077056
6	0.7290639290796029	2.433973636721014
7	0.4969776861519897	1.9356816700600008
8	0.346594060867272	1.5428017089621777
9	0.24805423188094455	1.242190369308572
>10	1.346833812566314	13.402049771165055
>50	0.08877933295333794	3.384914580258104
>100	0.040507403749015285	4.079099096940036
>500	0.0011392424790230167	0.39843273128118567
>1k	2.278484954232592E-4	0.1444005664763666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.141320139788746E-5
2	0.0	0.0	0.0	0.0	3.141320139788746E-5
3	0.0	0.0	0.0	0.0	3.141320139788746E-5
4	0.0	0.0	0.0	0.0	3.141320139788746E-5
5	0.0	0.0	0.0	3.141320139788746E-5	3.141320139788746E-5
6	0.0	0.0	0.0	3.141320139788746E-5	3.141320139788746E-5
7	0.0	0.0	0.0	3.141320139788746E-5	3.141320139788746E-5
8	0.0	0.0	0.0	3.141320139788746E-5	3.141320139788746E-5
9	0.0	0.0	0.0	3.141320139788746E-5	3.141320139788746E-5
10	0.0	0.0	0.0	3.141320139788746E-5	6.282640279577492E-5
11	0.0	0.0	0.0	3.141320139788746E-5	6.282640279577492E-5
12	0.0	0.0	0.0	3.141320139788746E-5	1.570660069894373E-4
13	0.0	0.0	0.0	3.141320139788746E-5	1.570660069894373E-4
14	0.0	0.0	0.0	3.141320139788746E-5	1.570660069894373E-4
15	0.0	0.0	0.0	3.141320139788746E-5	1.8847920838732478E-4
16	0.0	0.0	0.0	3.141320139788746E-5	2.1989240978521223E-4
17	0.0	0.0	0.0	3.141320139788746E-5	2.1989240978521223E-4
18	0.0	0.0	0.0	3.141320139788746E-5	2.513056111830997E-4
19	0.0	0.0	0.0	3.141320139788746E-5	2.513056111830997E-4
20	0.0	0.0	0.0	3.141320139788746E-5	2.8271881258098714E-4
21	0.0	0.0	0.0	6.282640279577492E-5	2.8271881258098714E-4
22	0.0	0.0	0.0	1.2565280559154984E-4	2.8271881258098714E-4
23	0.0	0.0	0.0	1.570660069894373E-4	2.8271881258098714E-4
24	0.0	0.0	0.0	2.8271881258098714E-4	2.8271881258098714E-4
25	0.0	0.0	0.0	4.08371618172537E-4	3.141320139788746E-4
26	0.0	0.0	0.0	6.910904307535242E-4	3.455452153767621E-4
27	0.0	0.0	0.0	7.539168335492991E-4	3.455452153767621E-4
28	0.0	0.0	0.0	0.001633486472690148	3.7695841677464956E-4
29	0.0	0.0	0.0	0.002638708917422547	3.7695841677464956E-4
30	0.0	0.0	0.0	0.004492087799897907	3.7695841677464956E-4
31	0.0	0.0	0.0	0.010617662072485962	3.7695841677464956E-4
32	0.0	0.0	0.0	0.017025955157655006	3.7695841677464956E-4
33	0.0	0.0	0.0	0.025224800722503632	4.08371618172537E-4
34	0.0	0.0	0.0	0.0370675776495072	4.08371618172537E-4
35	0.0	0.0	0.0	0.051077865472965014	4.08371618172537E-4
36	0.0	0.0	0.0	0.06841795264459889	4.08371618172537E-4
37	0.0	0.0	0.0	0.09094121804688421	4.08371618172537E-4
38	3.141320139788746E-5	0.0	0.0	0.11641732438057094	4.3978481957042447E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5060	0.0	20.918259	1
GGTATCA	2705	0.0	20.175085	1
TGCGATA	155	1.44355E-8	17.030348	13
TATCAAC	6690	0.0	15.787697	2
ATCAACG	6785	0.0	15.469597	3
TCAACGC	6825	0.0	15.282211	4
CAACGCA	6855	0.0	15.1497	5
AACGCAG	7140	0.0	14.664792	6
GTACATG	12265	0.0	14.353354	1
GTATAAA	460	0.0	14.351404	1
TTACGTA	110	0.0010170848	14.002831	4
TACATGG	12290	0.0	13.929366	2
GTCTAAT	190	1.7503044E-7	13.898201	1
TTATTCG	160	6.335693E-6	13.752996	28
TATTCGC	160	6.3387834E-6	13.752349	29
GCACCGT	475	0.0	13.429863	6
TACCGTA	165	8.7381195E-6	13.331419	7
GTACTAA	215	5.457332E-8	13.305643	1
GCGATAC	200	3.2794014E-7	13.199972	14
TATTAGA	285	7.8216544E-11	13.125255	2
>>END_MODULE