FastQCFastQC Report
Mon 6 Feb 2017
SRR2077148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183375
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56650.022.3315331
GGTATCA28400.022.1563551
GCACCGT4750.016.675546
TATCAAC79250.015.8248592
ATCAACG80200.015.4453683
TCAACGC80800.015.3581464
CAACGCA82150.015.0796895
CGCGTAA2203.0559022E-1015.00261410
GTACATG113950.014.5195821
AACGCAG85650.014.4627916
GCGATAC1554.5644792E-614.19579914
ATACCGT2950.014.1710716
CTTAACG1251.6712381E-414.08112721
TATACAG5650.014.0199215
TACATGG115650.013.983182
TACCGTC3400.013.5901787
ACCGTAC1302.3295626E-413.5403968
CGTGCCG3800.013.3181110
CGTCGTA2653.4924597E-1013.28533610
ACCGTCG3650.013.2621698
Produced by FastQC (version 0.11.5)