FastQCFastQC Report
Mon 6 Feb 2017
SRR2077147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612019
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.023.312991
GTATCAA44750.023.2110181
TATCAAC60500.016.9497682
TCAACGC59350.016.7586444
ATCAACG60750.016.4091133
CAACGCA61750.016.2496135
AACGCAG64200.015.6242986
TATACCG2303.6379788E-1115.3076065
TCGATCG1603.83714E-715.12235341
GTACATG108950.014.845861
GGACCGT3250.014.2136836
TACATGG110150.014.1842722
AACGCGC950.006242220813.894999530
ACATGGG116350.013.2392953
ACGCAGA75150.013.2013487
GTGTAGC13200.013.17038251
ATACCGT3051.8189894E-1112.9820546
GACCGTA1250.00266939812.3185257
GTCTTAT5200.012.2726931
CGCAGAG80750.012.2587658
Produced by FastQC (version 0.11.5)