##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077147_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3612019 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.2444732433578 31.0 30.0 34.0 23.0 34.0 2 29.368342746812793 31.0 30.0 34.0 23.0 34.0 3 29.476775454392683 31.0 30.0 34.0 23.0 34.0 4 32.885761951972015 37.0 35.0 37.0 26.0 37.0 5 32.85309185804394 37.0 35.0 37.0 25.0 37.0 6 32.91303174208109 37.0 35.0 37.0 26.0 37.0 7 32.75850846853242 37.0 35.0 37.0 25.0 37.0 8 32.64966241871928 37.0 33.0 37.0 25.0 37.0 9 34.0443167657756 38.0 35.0 39.0 25.0 39.0 10 34.007101845256074 38.0 35.0 39.0 24.0 39.0 11 34.01791269647253 38.0 35.0 39.0 25.0 39.0 12 33.93361884309025 38.0 35.0 39.0 23.0 39.0 13 33.87153085296617 38.0 34.0 39.0 23.0 39.0 14 34.83806480530695 39.0 34.0 41.0 22.0 41.0 15 34.87562551581262 39.0 34.0 41.0 22.0 41.0 16 34.84138427843264 39.0 34.0 41.0 22.0 41.0 17 34.684385381140025 39.0 34.0 41.0 19.0 41.0 18 34.66188190039975 39.0 34.0 41.0 19.0 41.0 19 34.78479819735168 39.0 34.0 41.0 21.0 41.0 20 34.76881406216302 39.0 34.0 41.0 21.0 41.0 21 34.716309078108395 39.0 34.0 41.0 20.0 41.0 22 34.641221433220586 39.0 34.0 41.0 19.0 41.0 23 34.60042209080296 39.0 34.0 41.0 19.0 41.0 24 34.60527948496395 39.0 34.0 41.0 19.0 41.0 25 34.50941149534374 39.0 34.0 41.0 19.0 41.0 26 34.45666703303609 39.0 34.0 41.0 18.0 41.0 27 34.39576480633131 39.0 34.0 41.0 18.0 41.0 28 34.28783154241437 39.0 34.0 41.0 17.0 41.0 29 34.27119514044638 39.0 34.0 41.0 17.0 41.0 30 34.116878953294545 39.0 34.0 41.0 16.0 41.0 31 34.10823392678721 39.0 34.0 41.0 16.0 41.0 32 33.984393216093274 39.0 33.0 41.0 16.0 41.0 33 33.92932899854624 39.0 33.0 41.0 16.0 41.0 34 33.85342269794262 39.0 33.0 41.0 15.0 41.0 35 33.789566721548255 38.0 33.0 41.0 15.0 41.0 36 33.699037297422855 38.0 33.0 41.0 11.0 41.0 37 33.609662905981395 38.0 33.0 40.0 9.0 41.0 38 33.52306203261943 38.0 33.0 40.0 9.0 41.0 39 33.40789458748694 38.0 33.0 40.0 9.0 41.0 40 33.348693625365755 38.0 33.0 40.0 8.0 41.0 41 33.23785423055637 38.0 33.0 40.0 8.0 41.0 42 33.0919391620033 38.0 33.0 40.0 2.0 41.0 43 33.01591519867421 38.0 32.0 40.0 2.0 41.0 44 32.87524041263349 38.0 32.0 40.0 2.0 41.0 45 32.73228989105539 38.0 32.0 40.0 2.0 41.0 46 32.60609731011935 38.0 32.0 40.0 2.0 41.0 47 32.44492761527555 38.0 32.0 40.0 2.0 41.0 48 32.29119918804414 38.0 32.0 40.0 2.0 41.0 49 32.11600049722883 38.0 32.0 40.0 2.0 41.0 50 31.909576057047317 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.573009459854127 1101 2 1.7653092924108549 1101 3 1.846742105644367 1101 4 2.103573674206565 1101 5 2.1481688791174065 1101 6 2.0725141342640683 1101 7 2.202755570059125 1101 8 2.1497140800344354 1101 9 2.375319805681116 1101 10 2.578051051896516 1101 11 2.41021316475549 1101 12 2.5646839443729306 1101 13 2.622692380364562 1101 14 2.973860721507428 1101 15 2.9832263497162756 1101 16 3.0238630664281985 1101 17 3.1216717517608146 1101 18 3.115670603131356 1101 19 3.02692847358815 1101 20 3.14164489534042 1101 21 3.1650611548082566 1101 22 3.1054777435852685 1101 23 3.112304581343402 1101 24 3.009581636277453 1101 25 3.1290425611505412 1101 26 3.1952348681226184 1101 27 3.2433139793788257 1101 28 3.327721901931959 1101 29 3.379967831501979 1101 30 3.365139282735079 1101 31 3.368345824665859 1101 32 3.459202809212478 1101 33 3.355808984133269 1101 34 3.4335650585031416 1101 35 3.4493096463434725 1101 36 3.3971588510919872 1101 37 3.4323760600754127 1101 38 3.4911438265530705 1101 39 3.533218514141147 1101 40 3.521280591787722 1101 41 3.561283007763933 1101 42 3.677107248425216 1101 43 3.5858206267340336 1101 44 3.5431325383787993 1101 45 3.5236594671720525 1101 46 3.545763696600254 1101 47 3.6865583056942484 1101 48 3.7257993921259427 1101 49 3.713473132110167 1101 50 3.7460822799109152 1102 1 0.9487539581379956 1102 2 1.1205188876056837 1102 3 1.1457629011218557 1102 4 1.2737197977680452 1102 5 1.3081401086752464 1102 6 1.2605250619846444 1102 7 1.3877503964269877 1102 8 1.2916760222916537 1102 9 1.5107690283744333 1102 10 1.6014640086903782 1102 11 1.3946653906791724 1102 12 1.5343503081928418 1102 13 1.611824219653883 1102 14 1.892758485488855 1102 15 1.8029084868049097 1102 16 1.8145949460970883 1102 17 2.018802278714176 1102 18 1.951820537514557 1102 19 1.9099522722960032 1102 20 1.780643860613992 1102 21 1.7965033363144087 1102 22 1.8717599210534672 1102 23 1.7411051819894752 1102 24 1.7164293800690942 1102 25 1.8174999354691366 1102 26 1.916842605857326 1102 27 1.831694127773737 1102 28 1.942713952204521 1102 29 1.9614983433130035 1102 30 2.0264570446975227 1102 31 1.8940192801473898 1102 32 2.00020674622035 1102 33 1.9455625425826923 1102 34 2.0141041257351233 1102 35 2.0219596816755967 1102 36 2.0949304036863765 1102 37 1.9998876692011933 1102 38 1.9893547593210883 1102 39 2.1388037655039582 1102 40 2.0800876279274405 1102 41 2.044117779619377 1102 42 1.9941677420654358 1102 43 1.9143716311757828 1102 44 1.8168885953139906 1102 45 1.8749030316784143 1102 46 1.9736946223018492 1102 47 1.8276946119732713 1102 48 1.8808446929537226 1102 49 1.9445994696833537 1102 50 1.9946422435693094 1103 1 0.5517437281905018 1103 2 0.6776645738269416 1103 3 0.6094511140496124 1103 4 0.7244220881899182 1103 5 0.743462849939057 1103 6 0.641549980507186 1103 7 0.66401816303933 1103 8 0.8008173404158612 1103 9 0.8163855411392333 1103 10 0.7919387280457997 1103 11 0.8351849226364436 1103 12 0.7794286170829352 1103 13 0.8402381251400328 1103 14 0.9501354771600461 1103 15 0.8138913086408692 1103 16 0.6480106621934141 1103 17 0.9684016812128178 1103 18 0.8439521915069506 1103 19 0.9105161642025763 1103 20 0.8070279589301279 1103 21 0.6954234205077867 1103 22 0.7330215768564727 1103 23 0.7933153711982399 1103 24 0.7343932357634628 1103 25 0.8571843234828975 1103 26 0.7992517358591371 1103 27 0.8065781355593629 1103 28 0.9233744854292212 1103 29 0.9985385614915288 1103 30 0.9960613132110083 1103 31 0.9151369924414325 1103 32 0.9943372006835034 1103 33 0.9440700339822996 1103 34 0.9754820000787774 1103 35 0.8418416525247707 1103 36 0.9720465543291397 1103 37 1.025682527068298 1103 38 0.9896940224027446 1103 39 1.0594626012816164 1103 40 0.9932356000440379 1103 41 0.9735063591956887 1103 42 1.0364985631771475 1103 43 1.0286674169805963 1103 44 0.9697672391669698 1103 45 0.9518896105304862 1103 46 0.9000823418383348 1103 47 0.9828404792555432 1103 48 0.9889864410978504 1103 49 1.0387591202355964 1103 50 1.088578057438582 1104 1 0.6894978829431295 1104 2 0.7375222690820706 1104 3 0.6721957300993893 1104 4 0.6567442131738233 1104 5 0.6839482242185753 1104 6 0.5851831889906052 1104 7 0.5959636586919999 1104 8 0.7359275124337898 1104 9 0.7768121278700804 1104 10 0.6807249555037629 1104 11 0.7876046960436867 1104 12 0.7522194049374491 1104 13 0.7743803865498009 1104 14 0.9694328894319995 1104 15 0.8200711988635945 1104 16 0.8154019153772367 1104 17 0.9606695414376958 1104 18 0.8344669726360578 1104 19 0.9243954749553325 1104 20 0.792363541952831 1104 21 0.7766497714359133 1104 22 0.9262243295816646 1104 23 0.8824500352677873 1104 24 0.8363631833703309 1104 25 0.8446380028694165 1104 26 0.8653269593648929 1104 27 0.9575495266733256 1104 28 0.8167244405126368 1104 29 0.9359077312084594 1104 30 0.9321061162243467 1104 31 0.9501893282904419 1104 32 0.9672528260002693 1104 33 0.9024392619774702 1104 34 0.9423159551711606 1104 35 0.98592431945643 1104 36 0.8868549795689944 1104 37 1.027388815464377 1104 38 0.988238872113385 1104 39 0.9238983322164742 1104 40 0.8941585816184059 1104 41 0.9961429400962203 1104 42 0.8798976251465902 1104 43 0.930024714623741 1104 44 1.0599611537734148 1104 45 1.0199640701928558 1104 46 0.9989814746631467 1104 47 1.0143266041786845 1104 48 1.0728635489830722 1104 49 1.1379425462537398 1104 50 1.0856910743808612 1105 1 0.510352328104485 1105 2 0.5007570386251672 1105 3 0.44828746521568164 1105 4 0.5580631697223808 1105 5 0.5001851759780251 1105 6 0.4491385750760273 1105 7 0.5129778272783483 1105 8 0.5585947194007517 1105 9 0.5830289250064595 1105 10 0.4926927458403725 1105 11 0.5065295285220799 1105 12 0.5950567033765708 1105 13 0.6588379073917139 1105 14 0.7238257101722212 1105 15 0.7034585876170851 1105 16 0.6000833881939798 1105 17 0.6559061294553601 1105 18 0.6107488401782675 1105 19 0.5280710247808074 1105 20 0.6210006434276494 1105 21 0.7509210749978905 1105 22 0.7107328082574185 1105 23 0.6680461358714567 1105 24 0.7184238637971561 1105 25 0.5791872970298826 1105 26 0.7290038556443434 1105 27 0.7078085637935061 1105 28 0.7359073198991908 1105 29 0.6624536806788228 1105 30 0.6767272142323648 1105 31 0.5682545629894378 1105 32 0.8103102561879751 1105 33 0.6175821583605128 1105 34 0.7113387483284086 1105 35 0.6695961685842136 1105 36 0.763247692264784 1105 37 0.7707563577909582 1105 38 0.6869601861399701 1105 39 0.7846584681790318 1105 40 0.7639617262974028 1105 41 0.7873267661020193 1105 42 0.8136543454050127 1105 43 0.7261574620953013 1105 44 0.6860607285564555 1105 45 0.8535029848220503 1105 46 0.8918318333542672 1105 47 0.6270441959455582 1105 48 0.8552775534637505 1105 49 0.7857006652305856 1105 50 0.7701870552919203 1106 1 0.3299373675748285 1106 2 0.2697911478796584 1106 3 0.25069271678226457 1106 4 0.2718537822091065 1106 5 0.27933281965106715 1106 6 0.20708042728144704 1106 7 0.3180430778069976 1106 8 0.2826049077981594 1106 9 0.39416493569728317 1106 10 0.39098466233488693 1106 11 0.38453654322441366 1106 12 0.3135389603152845 1106 13 0.43990302905689305 1106 14 0.41812621337656 1106 15 0.4658062332956945 1106 16 0.32518263424185534 1106 17 0.5182970360285637 1106 18 0.2194257083246356 1106 19 0.3774785914233476 1106 20 0.2752723275512494 1106 21 0.507794876141908 1106 22 0.4536142039893676 1106 23 0.37771107155484174 1106 24 0.45236429043607984 1106 25 0.4800520452272963 1106 26 0.4671515649305533 1106 27 0.5170912400686802 1106 28 0.4551686072019834 1106 29 0.44172485692707397 1106 30 0.486256321714265 1106 31 0.45117121233071344 1106 32 0.6530576679183042 1106 33 0.5110370267700759 1106 34 0.6610498385712518 1106 35 0.5487251771939441 1106 36 0.6063353588698703 1106 37 0.5703113120220991 1106 38 0.5987681281693895 1106 39 0.599591225338223 1106 40 0.6739365624784242 1106 41 0.6636373762708203 1106 42 0.6406076837194163 1106 43 0.5103443818945408 1106 44 0.6333415379826874 1106 45 0.6003031035539408 1106 46 0.4901935441689176 1106 47 0.47027060977472956 1106 48 0.6030602561196332 1106 49 0.6768388151932498 1106 50 0.6166247397641342 1107 1 -0.055777486268745236 1107 2 -0.1713036579208378 1107 3 -0.14598576984296585 1107 4 -0.22369833932052074 1107 5 -0.1972594501981817 1107 6 -0.24664699151609426 1107 7 -0.220874122858163 1107 8 -0.14445535424946598 1107 9 -0.14796414660577284 1107 10 -0.25916198101666765 1107 11 -0.23913868974159413 1107 12 -0.3106307459865505 1107 13 -0.1397864578712813 1107 14 -0.3792029017976546 1107 15 -0.2508360698063328 1107 16 -0.33652087790472507 1107 17 -0.2873607684524373 1107 18 -0.1982145970856024 1107 19 -0.2520301816119286 1107 20 -0.18661684236078457 1107 21 -0.26044306397967176 1107 22 -0.48132520789122424 1107 23 -0.5689660062245281 1107 24 -0.5013499293981596 1107 25 -0.45502572622071824 1107 26 -0.1332301376106102 1107 27 -0.15102240598940853 1107 28 -0.22826734233229473 1107 29 -0.15340388776945701 1107 30 -0.1886110330086197 1107 31 -0.2133023605041231 1107 32 -0.18800414621263428 1107 33 -0.25057357041357164 1107 34 -0.1857906825444786 1107 35 -0.12104513961158858 1107 36 -0.3813236965285327 1107 37 -0.23909491195478694 1107 38 -0.18946649958627404 1107 39 -0.08181423585335068 1107 40 -0.11393015852205934 1107 41 -0.094596237460145 1107 42 -0.12834222569839682 1107 43 -0.31457567399331765 1107 44 -0.12295234462492743 1107 45 -0.017819440327016878 1107 46 -0.1495167649846607 1107 47 -0.15221921282030593 1107 48 -0.35881930150391383 1107 49 -0.3493230221162129 1107 50 -0.20747261900280023 1108 1 -0.16618492887338476 1108 2 -0.23096422887510926 1108 3 -0.24777658293807292 1108 4 -0.31146987779802515 1108 5 -0.29367215898274424 1108 6 -0.419648490454243 1108 7 -0.40951521977218164 1108 8 -0.2596349498836332 1108 9 -0.32728662105667894 1108 10 -0.39397272387693505 1108 11 -0.33097270496124764 1108 12 -0.5841658008530075 1108 13 -0.5102361611053823 1108 14 -0.6237327981655341 1108 15 -0.594033962783918 1108 16 -0.5317444703724448 1108 17 -0.5824861633270544 1108 18 -0.5406029304363287 1108 19 -0.5686569847728293 1108 20 -0.7103124731699424 1108 21 -0.5992650116635119 1108 22 -0.6940560218326439 1108 23 -0.8590814660898332 1108 24 -0.6528636665682868 1108 25 -0.5441849665247815 1108 26 -0.6450640771436866 1108 27 -0.7068081111583311 1108 28 -0.7060624502963222 1108 29 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-1.552858785123263 2216 21 -1.6243578585537435 2216 22 -1.4597980338950975 2216 23 -1.4841897659826202 2216 24 -1.516481448238821 2216 25 -1.7193262883691034 2216 26 -1.654687857072794 2216 27 -1.7402695561567327 2216 28 -1.7457719913925018 2216 29 -1.9107744041151662 2216 30 -1.8564254047526347 2216 31 -1.9035177453935859 2216 32 -1.8410055631091993 2216 33 -1.8797560544375216 2216 34 -1.8510714684472092 2216 35 -1.8584761259393971 2216 36 -1.9289625252482345 2216 37 -2.1536023390759738 2216 38 -2.1256031158119377 2216 39 -2.079018600583307 2216 40 -2.099123824124117 2216 41 -2.017884186207752 2216 42 -2.0050436360828456 2216 43 -2.1747312569642183 2216 44 -2.4193268179531238 2216 45 -2.3871590914148832 2216 46 -2.5563931008860443 2216 47 -2.2814762671800928 2216 48 -2.4805871830070174 2216 49 -2.4781472194499052 2216 50 -2.6229357982895074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 169336.0 3 8337.0 4 15864.0 5 12678.0 6 10408.0 7 8998.0 8 8302.0 9 7695.0 10 7548.0 11 7328.0 12 7702.0 13 7973.0 14 8497.0 15 8992.0 16 9617.0 17 10447.0 18 11307.0 19 12957.0 20 15881.0 21 20184.0 22 24462.0 23 28309.0 24 30445.0 25 33245.0 26 37082.0 27 42098.0 28 48718.0 29 55773.0 30 65526.0 31 79022.0 32 96839.0 33 119802.0 34 153981.0 35 201703.0 36 266097.0 37 365508.0 38 571742.0 39 1021616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.011646253165104 16.973575779340216 13.015346009821313 24.999431957673366 2 16.323120332943702 18.85350798578261 38.91545551134559 25.907916169928097 3 20.089493444159025 22.95014091088097 27.727170816024906 29.233194828935105 4 13.759737401933126 15.153653347936896 35.23440158835913 35.852207661770855 5 15.34640034562579 36.35078800851428 33.07815072465857 15.224660921201366 6 34.55910034147732 34.32812460611822 16.521995189344423 14.590779863060032 7 30.369139461272393 30.68359067161316 19.97812248362164 18.969147383492807 8 27.331538792471793 34.377009266623396 19.073797514957047 19.217654425947764 9 26.98067911214337 15.989957260458556 18.39093992639978 38.638423700998295 10 15.670797827920445 28.45972637115638 32.11517395055625 23.75430185036693 11 36.532662356170206 22.279882013939726 22.021119272118302 19.16633635777176 12 24.35462911586772 23.921487043550503 28.616685055905965 23.107198784675813 13 28.891704409820427 20.917680083387186 24.97363680758706 25.216978699205324 14 23.01769835758422 20.352349471996554 25.521500874350334 31.1084512960689 15 24.79997939131244 27.839566907322954 22.416712094418813 24.943741606945792 16 24.87374706199282 27.06121719715846 23.84929148493843 24.215744255910295 17 23.895598015295256 26.896616295587155 25.270636519365052 23.937149169752534 18 24.026587404916373 26.61117236106571 25.7277602645998 23.634479969418113 19 24.83844131292561 26.085477406716773 25.834513262813672 23.241568017543944 20 25.07359144247539 25.62023906656268 25.59078863706571 23.715380853896225 21 25.13781178574427 25.483162094367856 25.518988309792306 23.860037810095566 22 25.09135545223304 25.19130058908611 25.378915926383634 24.338428032297212 23 24.599377264618212 25.378738351413343 25.462591554289894 24.55929282967855 24 24.52338672429037 25.532762028204324 25.493223775152302 24.45062747235301 25 24.743527789661755 25.31111386967133 25.507125582715055 24.43823275795186 26 24.539348632038617 25.439842394888363 25.627012319133723 24.393796653939294 27 24.499815611078184 25.64682276137857 25.391975383039934 24.46138624450331 28 24.571712979038676 25.622730533963807 25.327095474217426 24.47846101278009 29 24.48340701159955 25.680464092721007 25.546989775928292 24.28913911975115 30 24.73322126405172 25.6985373268567 25.306941359245037 24.26130004984654 31 24.701321473634934 25.451407692829747 25.308720119335675 24.538550714199644 32 24.594222665914703 25.70715335940088 25.234785219721957 24.463838754962456 33 24.532684391603276 25.550030748092787 25.281164992603838 24.6361198677001 34 24.83427345682911 25.455325575237026 25.408651795320104 24.301749172613764 35 24.678940609275983 25.621148810889093 25.37045101226508 24.329459567569845 36 24.770004313068377 25.612451554158927 25.32170032956053 24.29584380321217 37 24.809131757262133 25.32012676532348 25.461878985655794 24.408862491758594 38 24.742723506400303 25.441905080049114 25.376147332808713 24.439224080741873 39 24.72184534141154 25.59931435700748 25.351838011856927 24.327002289724053 40 24.905081787836398 25.38625188714525 25.39993628720619 24.308730037812158 41 24.692794186646193 25.469512630849483 25.41638900014015 24.421304182364175 42 24.838148331047908 25.537707595970595 25.23559371277412 24.388550360207383 43 24.606557095543575 25.27019339371544 25.450276161993617 24.67297334874737 44 24.679038096111178 25.469315474220434 25.26904577067 24.582600658998384 45 24.676586355936266 25.55951605859206 25.242395801860628 24.52150178361105 46 24.76150914971864 25.222456688377864 25.268482210111216 24.747551951792282 47 24.610953562566465 25.566814737681675 25.356173564338885 24.466058135412975 48 24.66470473718185 25.492741627068533 25.28413315606367 24.558420479685942 49 24.708478950778996 25.377894579430972 25.204455201519778 24.709171268270257 50 24.733793684047285 25.554117055730867 25.27726968733168 24.43481957289017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2434.0 1 1825.5 2 1217.0 3 1177.0 4 1137.0 5 1065.5 6 994.0 7 967.5 8 941.0 9 995.5 10 1050.0 11 1247.5 12 1445.0 13 2171.0 14 2897.0 15 3862.0 16 4827.0 17 5126.5 18 5426.0 19 5657.5 20 5889.0 21 6836.0 22 7783.0 23 9978.5 24 12174.0 25 16097.5 26 20021.0 27 25819.0 28 31617.0 29 39191.0 30 46765.0 31 56056.0 32 65347.0 33 76789.0 34 88231.0 35 100897.5 36 113564.0 37 127728.0 38 141892.0 39 157001.5 40 172111.0 41 187358.5 42 202606.0 43 215631.0 44 228656.0 45 242566.5 46 256477.0 47 265873.5 48 275270.0 49 279626.0 50 283982.0 51 284304.0 52 284626.0 53 279414.5 54 274203.0 55 262293.0 56 250383.0 57 233594.5 58 216806.0 59 199463.5 60 182121.0 61 160538.0 62 138955.0 63 119020.5 64 99086.0 65 83345.0 66 67604.0 67 56132.5 68 44661.0 69 37581.5 70 30502.0 71 24606.5 72 18711.0 73 15495.0 74 12279.0 75 9758.5 76 7238.0 77 5723.5 78 4209.0 79 3392.0 80 2575.0 81 2020.0 82 1465.0 83 1146.0 84 827.0 85 641.0 86 455.0 87 352.0 88 249.0 89 185.5 90 122.0 91 93.0 92 64.0 93 52.0 94 40.0 95 31.0 96 22.0 97 22.0 98 22.0 99 31.5 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08673819268392553 2 0.09637269349912057 3 0.10271263800107364 4 0.10429070278976937 5 0.09667723231799168 6 0.058582194611933106 7 0.053155866566593366 8 0.06040942752515976 9 0.0622089750912163 10 0.045791564219346576 11 0.04878158171371746 12 0.05863756530627331 13 0.05182696990242853 14 0.05287901309489236 15 0.05235299149866044 16 0.055481435728881826 17 0.05567523315907254 18 0.05645042287983535 19 0.06478371237803567 20 0.07098523014413823 21 0.0811180672083951 22 0.08413577004993605 23 0.059218957596845416 24 0.07906935151780763 25 0.08446799421597727 26 0.08302835616313203 27 0.07751897207628199 28 0.0973416806500741 29 0.08540929601976069 30 0.08145029137443628 31 0.05631199614398485 32 0.05290669844206246 33 0.05645042287983535 34 0.05135631900053682 35 0.05800080232136099 36 0.057031815170407466 37 0.06088007842705147 38 0.04977825421184108 39 0.05473393135528911 40 0.05728098329493837 41 0.043549051098568416 42 0.04042060686834704 43 0.04033755082683674 44 0.03842726187209979 45 0.030066287026729373 46 0.027103954879528595 47 0.03153361042674471 48 0.026245709117255475 49 0.02655024793612658 50 0.027796088558781115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3612019.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.18815172247527 #Duplication Level Percentage of deduplicated Percentage of total 1 74.18587383183213 41.683671345273915 2 13.335455560836323 14.985892006811957 3 5.140006378508531 8.66422374750384 4 2.457485289552341 5.52326225220472 5 1.3390273942519255 3.7618737194388947 6 0.8398071159690266 2.831232578980923 7 0.5572548171740183 2.191778275081777 8 0.39055468427195617 1.7555636684636877 9 0.2961771085947675 1.4977479883002145 >10 1.3861483919609339 13.455225981816273 >50 0.055619751678737 2.097238064041418 >100 0.01629182505835004 1.3850732512747315 >500 1.9856687406295416E-4 0.0874159227324874 >1k 9.928343695153453E-5 0.0798011980752461 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.7685347170100712E-5 0.0 0.0 0.0 2.7685347170100712E-5 7 2.7685347170100712E-5 0.0 0.0 0.0 2.7685347170100712E-5 8 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 9 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 10 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 11 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 12 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 13 2.7685347170100712E-5 0.0 0.0 0.0 5.5370694340201424E-5 14 2.7685347170100712E-5 0.0 0.0 2.7685347170100712E-5 5.5370694340201424E-5 15 2.7685347170100712E-5 0.0 0.0 2.7685347170100712E-5 8.305604151030214E-5 16 2.7685347170100712E-5 0.0 0.0 2.7685347170100712E-5 1.3842673585050356E-4 17 2.7685347170100712E-5 0.0 0.0 2.7685347170100712E-5 1.3842673585050356E-4 18 2.7685347170100712E-5 0.0 0.0 2.7685347170100712E-5 1.3842673585050356E-4 19 2.7685347170100712E-5 0.0 0.0 5.5370694340201424E-5 1.3842673585050356E-4 20 2.7685347170100712E-5 0.0 0.0 5.5370694340201424E-5 1.3842673585050356E-4 21 2.7685347170100712E-5 0.0 0.0 5.5370694340201424E-5 1.3842673585050356E-4 22 2.7685347170100712E-5 0.0 0.0 5.5370694340201424E-5 1.3842673585050356E-4 23 2.7685347170100712E-5 0.0 0.0 5.5370694340201424E-5 2.214827773608057E-4 24 2.7685347170100712E-5 0.0 0.0 1.9379743019070497E-4 2.214827773608057E-4 25 2.7685347170100712E-5 0.0 0.0 1.9379743019070497E-4 2.214827773608057E-4 26 2.7685347170100712E-5 0.0 0.0 4.1528020755151067E-4 2.214827773608057E-4 27 2.7685347170100712E-5 0.0 0.0 5.537069434020142E-4 2.214827773608057E-4 28 2.7685347170100712E-5 0.0 0.0 0.0011350992339741292 2.214827773608057E-4 29 2.7685347170100712E-5 0.0 0.0 0.002187142426437956 2.214827773608057E-4 30 2.7685347170100712E-5 0.0 0.0 0.0039036339509842003 2.214827773608057E-4 31 2.7685347170100712E-5 0.0 0.0 0.008831625747262126 2.214827773608057E-4 32 2.7685347170100712E-5 0.0 0.0 0.016029816011488313 2.214827773608057E-4 33 2.7685347170100712E-5 0.0 0.0 0.023560230441755706 2.214827773608057E-4 34 2.7685347170100712E-5 0.0 0.0 0.03396992097771357 2.768534717010071E-4 35 2.7685347170100712E-5 0.0 0.0 0.047812594562763926 2.768534717010071E-4 36 2.7685347170100712E-5 0.0 0.0 0.06417463474029345 2.768534717010071E-4 37 2.7685347170100712E-5 0.0 0.0 0.08623985643486372 2.768534717010071E-4 38 2.7685347170100712E-5 0.0 0.0 0.11099055680493375 3.0453881887110783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2105 0.0 23.31299 1 GTATCAA 4475 0.0 23.211018 1 TATCAAC 6050 0.0 16.949768 2 TCAACGC 5935 0.0 16.758644 4 ATCAACG 6075 0.0 16.409113 3 CAACGCA 6175 0.0 16.249613 5 AACGCAG 6420 0.0 15.624298 6 TATACCG 230 3.6379788E-11 15.307606 5 TCGATCG 160 3.83714E-7 15.122353 41 GTACATG 10895 0.0 14.84586 1 GGACCGT 325 0.0 14.213683 6 TACATGG 11015 0.0 14.184272 2 AACGCGC 95 0.0062422208 13.8949995 30 ACATGGG 11635 0.0 13.239295 3 ACGCAGA 7515 0.0 13.201348 7 GTGTAGC 1320 0.0 13.1703825 1 ATACCGT 305 1.8189894E-11 12.982054 6 GACCGTA 125 0.002669398 12.318525 7 GTCTTAT 520 0.0 12.272693 1 CGCAGAG 8075 0.0 12.258765 8 >>END_MODULE