FastQCFastQC Report
Mon 6 Feb 2017
SRR2077147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612019
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.025.7403891
GTATCAA58300.022.8329261
TATACCG1605.2750693E-1119.2528425
TATCAAC79600.016.6680582
ATCAACG81600.016.0707763
TCAACGC81450.016.0193314
CAACGCA82950.015.7563955
AACGCAG87350.014.9362816
GTGGTAT14250.013.7419991
GTACATG107250.013.6836921
TAGAGGT8950.013.5213624
TATACTG8650.013.4817595
GTATATG7000.013.2015841
TACATGG107800.013.1650262
ATACCGA1353.2082698E-413.0378786
ACGCAGA103350.012.5180197
GAGTACT47600.012.43581412
ACCGTCC3950.012.2548048
ACATGGG111950.012.2052963
TAACGCA1455.856925E-412.1395554
Produced by FastQC (version 0.11.5)