FastQCFastQC Report
Mon 6 Feb 2017
SRR2077146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926295
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC34650.11840911459712708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18800.022.8265231
GTATCAA38600.022.235161
CGATCGT1800.020.7812925
AACTCGA1901.8189894E-1218.52437837
AAACTCG1901.8189894E-1218.52311136
CCGCGAT2050.018.24667422
ACTCGAG2201.8189894E-1216.99996838
GATCGTT2109.094947E-1216.7647426
TATCAAC51300.016.6436142
TCAACGC50000.016.6359524
ATCAACG50600.016.5262283
GCGCGAC800.00199133316.49742711
ATCGTTC2151.2732926E-1116.37486327
CAACGCA51700.016.2558445
TATAGGC8100.016.025215
CGCGATC2201.8189894E-1116.00243223
AACGCAG54000.015.7652886
GTATTAA4200.015.7193631
TATCATA5750.015.3082452
GTACATG91350.015.2495891
Produced by FastQC (version 0.11.5)