##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077146_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2926295
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.220365684252613	31.0	28.0	33.0	16.0	34.0
2	28.240793221462635	31.0	28.0	34.0	16.0	34.0
3	28.333153356035535	31.0	28.0	34.0	16.0	34.0
4	31.706450648345434	35.0	32.0	37.0	17.0	37.0
5	31.612951530860695	35.0	32.0	37.0	17.0	37.0
6	31.755052378519597	35.0	32.0	37.0	17.0	37.0
7	31.51213667794942	35.0	32.0	37.0	17.0	37.0
8	31.454377976246413	35.0	32.0	37.0	16.0	37.0
9	32.757915384470806	37.0	32.0	39.0	15.0	39.0
10	32.65804882966345	37.0	32.0	39.0	13.0	39.0
11	32.71165483999392	37.0	32.0	39.0	13.0	39.0
12	32.60317568802872	37.0	32.0	39.0	11.0	39.0
13	32.52917836376715	37.0	32.0	39.0	10.0	39.0
14	33.35343155765225	38.0	32.0	40.0	10.0	41.0
15	33.40053241385438	38.0	32.0	40.0	10.0	41.0
16	33.35579768956992	38.0	32.0	40.0	10.0	41.0
17	33.17894128924117	38.0	32.0	40.0	10.0	41.0
18	33.13578022721564	38.0	32.0	40.0	10.0	41.0
19	33.265658793798984	38.0	32.0	40.0	10.0	41.0
20	33.26264303496401	38.0	32.0	40.0	10.0	41.0
21	33.19941017566582	38.0	32.0	40.0	9.0	41.0
22	33.11965266659718	38.0	32.0	40.0	8.0	41.0
23	33.06340748284093	38.0	32.0	40.0	8.0	41.0
24	33.05717126947215	38.0	32.0	40.0	8.0	41.0
25	32.95133675859748	38.0	32.0	40.0	2.0	41.0
26	32.90019256431768	38.0	32.0	40.0	2.0	41.0
27	32.841417218701466	38.0	32.0	40.0	2.0	41.0
28	32.72761973758627	38.0	32.0	40.0	2.0	41.0
29	32.718985611498496	38.0	32.0	40.0	2.0	41.0
30	32.535916235376135	38.0	31.0	40.0	2.0	41.0
31	32.54156638343024	38.0	31.0	40.0	2.0	41.0
32	32.41034550515242	38.0	31.0	40.0	2.0	41.0
33	32.36543923288664	38.0	31.0	40.0	2.0	41.0
34	32.28645129763062	38.0	31.0	40.0	2.0	41.0
35	32.20457506847396	38.0	31.0	40.0	2.0	41.0
36	32.100332673226724	38.0	30.0	40.0	2.0	41.0
37	32.01176197204998	38.0	30.0	40.0	2.0	41.0
38	31.918416974365197	38.0	30.0	40.0	2.0	41.0
39	31.809848289389826	38.0	30.0	40.0	2.0	41.0
40	31.739730956721726	38.0	30.0	40.0	2.0	41.0
41	31.62682436323064	38.0	30.0	40.0	2.0	41.0
42	31.483672698753885	38.0	30.0	40.0	2.0	41.0
43	31.372677737548674	38.0	30.0	40.0	2.0	41.0
44	31.237626418389123	37.0	30.0	40.0	2.0	41.0
45	31.08191245243559	37.0	30.0	40.0	2.0	41.0
46	30.93341887950463	37.0	30.0	40.0	2.0	41.0
47	30.781562351027492	37.0	30.0	40.0	2.0	41.0
48	30.584212118053717	37.0	29.0	40.0	2.0	41.0
49	30.39607216634003	37.0	29.0	40.0	2.0	41.0
50	30.181569185608424	37.0	29.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	2.390140001038553
1101	2	2.5734876773330093
1101	3	2.651802196264054
1101	4	2.934758667112906
1101	5	2.917395398819039
1101	6	3.1333584438759345
1101	7	3.0229681624056646
1101	8	3.13605192416545
1101	9	3.5517707540039254
1101	10	3.7164644637942175
1101	11	3.5972040707239614
1101	12	3.8191788691240163
1101	13	3.691326970508335
1101	14	4.212863657542194
1101	15	4.192154653088288
1101	16	4.118199588836212
1101	17	4.266617186611754
1101	18	4.304800411042308
1101	19	4.187920944919611
1101	20	4.241964454925686
1101	21	4.2715746987231
1101	22	4.164126608265967
1101	23	4.287022826918452
1101	24	4.252743193230273
1101	25	4.147757283989911
1101	26	4.341359937209518
1101	27	4.281383361695674
1101	28	4.410619241536928
1101	29	4.365278433949378
1101	30	4.492440979547489
1101	31	4.51628483784954
1101	32	4.572303228958802
1101	33	4.646920226950833
1101	34	4.628326425178635
1101	35	4.550977790505769
1101	36	4.685922367462489
1101	37	4.691051491670869
1101	38	4.7698028871435625
1101	39	4.763111168153376
1101	40	4.777041608584458
1101	41	4.91132506561064
1101	42	4.897066231250676
1101	43	4.689430521225859
1101	44	4.7191881020013895
1101	45	4.773366280215388
1101	46	4.636357620317302
1101	47	4.891612428351099
1101	48	4.767563478284753
1101	49	4.767854161088529
1101	50	4.861015732067415
1102	1	1.6331921379819399
1102	2	1.6595259016698662
1102	3	1.6378118612858614
1102	4	1.7757673899717439
1102	5	1.8486643156849283
1102	6	1.9832632461591118
1102	7	1.952257163045548
1102	8	2.0608098079103883
1102	9	2.252051734481526
1102	10	2.3307308721512072
1102	11	2.217005784675436
1102	12	2.2634715206454104
1102	13	2.39392007780107
1102	14	2.6359698118292343
1102	15	2.3527302361497675
1102	16	2.5611333665564118
1102	17	2.862733610475175
1102	18	2.769283811308469
1102	19	2.7616725789835144
1102	20	2.6896103233447732
1102	21	2.691944164006358
1102	22	2.698086578116751
1102	23	2.601121314354316
1102	24	2.5422923486204283
1102	25	2.730072590605076
1102	26	2.7537000148594473
1102	27	2.6589458486918645
1102	28	2.767123110293632
1102	29	2.594634269274991
1102	30	2.662611020335291
1102	31	2.821339542187026
1102	32	3.019284774341614
1102	33	2.9274600470762238
1102	34	2.926776861517858
1102	35	2.903648429959624
1102	36	2.9511527263088055
1102	37	2.9035091434081792
1102	38	2.9521309522156187
1102	39	2.9111523524522305
1102	40	2.940151315611029
1102	41	2.901922671731562
1102	42	2.977120248594325
1102	43	2.834416742092408
1102	44	2.7531046930987344
1102	45	2.671846355431402
1102	46	2.766147537603242
1102	47	2.619540494208291
1102	48	2.5467425599114044
1102	49	2.580805957675544
1102	50	2.6105381732116726
1103	1	0.39020446699851163
1103	2	0.48454429456234394
1103	3	0.3334551840598401
1103	4	0.46557087777155104
1103	5	0.5717596435801724
1103	6	0.6202899850555852
1103	7	0.5294928226499103
1103	8	0.6867755545659833
1103	9	0.7317793561804606
1103	10	0.5877627074997207
1103	11	0.7025041799131841
1103	12	0.8147528780606095
1103	13	0.6611216464258689
1103	14	0.7283556335447443
1103	15	0.8763701627924263
1103	16	0.6152915696737011
1103	17	0.8603370941031017
1103	18	0.7055198108642244
1103	19	0.7821696747387463
1103	20	0.6810758521044917
1103	21	0.569485497980132
1103	22	0.666669488202146
1103	23	0.7781517064597807
1103	24	0.6186686020854779
1103	25	0.7983341536034771
1103	26	0.7952451193057115
1103	27	0.6426329738926313
1103	28	0.7885892084578359
1103	29	0.8229525021140702
1103	30	0.9557301335526347
1103	31	0.7440612054344484
1103	32	1.0318941521516436
1103	33	0.9329466257614385
1103	34	1.0298521531403395
1103	35	0.8510940306898149
1103	36	0.9971302782415989
1103	37	1.0254016747944874
1103	38	0.9971496495421803
1103	39	0.9825668941408132
1103	40	1.0306250094055933
1103	41	0.9943370764248058
1103	42	0.984957509409206
1103	43	0.7687171046508539
1103	44	0.7406869960072626
1103	45	0.7552478199848416
1103	46	0.7354634573070626
1103	47	0.8062514391209419
1103	48	0.8179239840202115
1103	49	0.7793804732729939
1103	50	0.868181526429062
1104	1	0.45084843060310575
1104	2	0.5733866063190476
1104	3	0.4413421771500978
1104	4	0.45003458694786147
1104	5	0.6381110203162379
1104	6	0.4157907045175122
1104	7	0.6288704998967027
1104	8	0.6817035958622739
1104	9	0.6561398450197871
1104	10	0.40925835055467275
1104	11	0.5953409116812622
1104	12	0.63893787323655
1104	13	0.5609624630992585
1104	14	0.737652432835759
1104	15	0.647422058113797
1104	16	0.6627997348043735
1104	17	0.7836775381872343
1104	18	0.5465746985763573
1104	19	0.6400130423117432
1104	20	0.6896055558689298
1104	21	0.567051411896955
1104	22	0.7154368854699626
1104	23	0.7514734168247088
1104	24	0.7372330274060559
1104	25	0.6680164161956412
1104	26	0.5408953331981721
1104	27	0.6898661801072805
1104	28	0.6167605518818959
1104	29	0.6906206234588979
1104	30	0.7883492409453581
1104	31	0.7216440376187805
1104	32	0.8254965244901271
1104	33	0.702827420367683
1104	34	0.6716679829338261
1104	35	0.5976303314294427
1104	36	0.7869411923219829
1104	37	0.7687859102794263
1104	38	0.8428885230891581
1104	39	0.829379794097374
1104	40	0.7987359007905148
1104	41	0.7782633414698168
1104	42	0.776951599136261
1104	43	0.8963861267710982
1104	44	0.870124392254759
1104	45	0.9097575400651756
1104	46	0.8301831896544343
1104	47	0.8898730066870222
1104	48	0.9004697644017128
1104	49	1.045255134080545
1104	50	1.1082468863968025
1105	1	0.43218573452325515
1105	2	0.3585561522698555
1105	3	0.2735184420578811
1105	4	0.36263305472502694
1105	5	0.29194857300823074
1105	6	0.2534273790625434
1105	7	0.32111092676801434
1105	8	0.3707748282391705
1105	9	0.3918288477516896
1105	10	0.39525231933716043
1105	11	0.4444765794942782
1105	12	0.2969705396260949
1105	13	0.35150795094260445
1105	14	0.42668930470450306
1105	15	0.4427382753561915
1105	16	0.3531762695149254
1105	17	0.46246624514530055
1105	18	0.25435895481206217
1105	19	0.21033510833896685
1105	20	0.3331674199569292
1105	21	0.3692198819536472
1105	22	0.3581410601085011
1105	23	0.4252371089799638
1105	24	0.4176513222638505
1105	25	0.3325897530764692
1105	26	0.5197054415921158
1105	27	0.47531599135825786
1105	28	0.473775097448069
1105	29	0.44177450137085117
1105	30	0.42986107507488214
1105	31	0.3265161900633444
1105	32	0.47996178500188336
1105	33	0.44817415185853093
1105	34	0.4309809156821487
1105	35	0.42561853999693255
1105	36	0.5725225791773738
1105	37	0.4937494502074813
1105	38	0.4444740642579035
1105	39	0.5841449658275799
1105	40	0.5560168880583412
1105	41	0.48915777504985414
1105	42	0.5032106934232559
1105	43	0.6021940142147173
1105	44	0.5143897425360962
1105	45	0.5713984567876516
1105	46	0.6402154082546652
1105	47	0.5060842150406764
1105	48	0.5445379497646137
1105	49	0.5681249413652907
1105	50	0.6473486153787569
1106	1	0.28035336910370745
1106	2	0.1756318302903317
1106	3	0.11779405869450699
1106	4	0.2326564351610294
1106	5	0.23215262566348116
1106	6	0.15933802558121357
1106	7	0.20910020753414926
1106	8	0.21760607790461606
1106	9	0.29705813695724004
1106	10	0.26442198278262197
1106	11	0.2072769033062798
1106	12	0.29066209722577696
1106	13	0.3698486368984959
1106	14	0.45279768996152825
1106	15	0.4103545988039201
1106	16	0.3422880214991224
1106	17	0.556930558045849
1106	18	0.17647779791757046
1106	19	0.394757008680223
1106	20	0.18407238864282505
1106	21	0.42091097065853234
1106	22	0.364639719465643
1106	23	0.3114935384695201
1106	24	0.25679108106631787
1106	25	0.34918668221531135
1106	26	0.33561661644295526
1106	27	0.4711896129153246
1106	28	0.3740526195563376
1106	29	0.392726681053837
1106	30	0.35849852033862817
1106	31	0.2700400890990693
1106	32	0.3635008666354622
1106	33	0.3839294826257458
1106	34	0.44582822247420495
1106	35	0.43694709377801644
1106	36	0.4927724472681305
1106	37	0.2939908406525973
1106	38	0.5425302537734282
1106	39	0.43700285177596143
1106	40	0.5700374158482653
1106	41	0.39195495493791555
1106	42	0.48239279874349705
1106	43	0.2019641549665785
1106	44	0.4343864369842301
1106	45	0.40850296366952676
1106	46	0.36818165443381545
1106	47	0.39316277455694504
1106	48	0.4319924245642106
1106	49	0.43790835646266046
1106	50	0.5271411442058813
1107	1	0.12111746167962067
1107	2	-0.004778075677901228
1107	3	-0.0014201392212775943
1107	4	-0.08184287326539064
1107	5	-0.06835893349493105
1107	6	-0.04132536327474057
1107	7	-0.02437109836376905
1107	8	-0.024919495776412504
1107	9	-0.05643043903416611
1107	10	7.142844609617782E-4
1107	11	-0.15370769731318745
1107	12	-0.18183294799381144
1107	13	-0.04794323751824692
1107	14	-0.3014301469656786
1107	15	-0.17364366306681234
1107	16	-0.17707167631272824
1107	17	-0.06526921048870804
1107	18	-0.008094594054526283
1107	19	-0.11051319258179859
1107	20	-0.010518838765563032
1107	21	-0.03480295824068946
1107	22	-0.45508753452106276
1107	23	-0.5055565516446165
1107	24	-0.39814120288857424
1107	25	-0.41913685275641654
1107	26	-0.14515892109832862
1107	27	-0.08710400946753794
1107	28	-0.24817375125198282
1107	29	-0.1441167410421258
1107	30	-0.21407825630406307
1107	31	-0.28113276323778535
1107	32	-0.24057856210851014
1107	33	-0.21799841238166806
1107	34	-0.15534197818905682
1107	35	-0.04283804367704391
1107	36	-0.39044434073398904
1107	37	-0.24348526220838806
1107	38	-0.29427099815865887
1107	39	-0.07411180915624982
1107	40	-0.03112371780207468
1107	41	-0.01098587938123785
1107	42	-0.1638428939805685
1107	43	-0.3558416877823376
1107	44	-0.0241416446667877
1107	45	-0.003959388980820933
1107	46	-0.15274552462172863
1107	47	-0.0773950092598028
1107	48	-0.15981882246655488
1107	49	-0.1849528763260544
1107	50	-0.05826164928947719
1108	1	-0.3662008469573088
1108	2	-0.4124966897194504
1108	3	-0.38996897957505183
1108	4	-0.4711613635306193
1108	5	-0.5165290283957162
1108	6	-0.5646872189777881
1108	7	-0.5494198268816
1108	8	-0.4813754568003219
1108	9	-0.5634800555125636
1108	10	-0.5882022691084501
1108	11	-0.5014166581470647
1108	12	-0.6491728165823787
1108	13	-0.5880168306136753
1108	14	-0.6690150455817658
1108	15	-0.6547489385279874
1108	16	-0.6309249711005052
1108	17	-0.8051368740929234
1108	18	-0.6949174610572086
1108	19	-0.6084534772337022
1108	20	-0.6894345682806602
1108	21	-0.6157848778163526
1108	22	-0.8178101763382841
1108	23	-0.9405337892463805
1108	24	-0.7555755181919395
1108	25	-0.5784104068354026
1108	26	-0.7313551704739822
1108	27	-0.8001817514241836
1108	28	-0.8908976880117017
1108	29	-0.8368251216745008
1108	30	-0.9182127386045558
1108	31	-0.8579554788835466
1108	32	-0.7803969506282584
1108	33	-0.7433220218740999
1108	34	-0.7304310685508142
1108	35	-0.727939225774545
1108	36	-0.9283595842252197
1108	37	-0.8381706164087959
1108	38	-0.8015390731173291
1108	39	-0.8013541198045431
1108	40	-0.8495908590567574
1108	41	-0.6256896150380697
1108	42	-0.42667350373031
1108	43	-0.5531408918131397
1108	44	-0.6387270696685938
1108	45	-0.5024253025627488
1108	46	-0.5049433111109352
1108	47	-0.5462032080957009
1108	48	-0.7066480632770897
1108	49	-0.6539982850822206
1108	50	-0.6918724702998347
1109	1	-0.14088326453678945
1109	2	-0.16204629160624506
1109	3	-0.23556138831350992
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2210	41	-0.4033538334719253
2210	42	-0.5076228615118126
2210	43	-0.5377101515570182
2210	44	-0.47686184703021084
2210	45	-0.32905471876524217
2210	46	-0.3312519617418239
2210	47	-0.36761285197509963
2210	48	-0.17777291315255184
2210	49	-0.08836398366172915
2210	50	-0.012480700144614332
2211	1	-0.19762906937958746
2211	2	-0.24127083277954142
2211	3	-0.30298022502599764
2211	4	-0.33716966623900646
2211	5	-0.49484063998878725
2211	6	-0.41145597045813886
2211	7	-0.4350511857685788
2211	8	-0.34287298321822135
2211	9	-0.30594241219269236
2211	10	-0.40592983096538404
2211	11	-0.3938956576510009
2211	12	-0.44887713542412655
2211	13	-0.44874478160316755
2211	14	-0.7385856905205728
2211	15	-0.49066459921829875
2211	16	-0.47725468637828783
2211	17	-0.43164261102573676
2211	18	-0.6075410636103555
2211	19	-0.4672290619546189
2211	20	-0.6609718636819295
2211	21	-0.49822819662880136
2211	22	-0.5312367227264119
2211	23	-0.5785125642253064
2211	24	-0.5243658952466532
2211	25	-0.548456965362611
2211	26	-0.6851481205043015
2211	27	-0.5359436956502819
2211	28	-0.2647814146734717
2211	29	-0.3760572477699995
2211	30	-0.7959920115606032
2211	31	-0.5822173228885674
2211	32	-0.3763917818355935
2211	33	-0.6190707213586606
2211	34	-0.42700416960958165
2211	35	-0.2948991570382731
2211	36	-0.2836224655308506
2211	37	-0.19523060820241867
2211	38	-0.31404833591223635
2211	39	-0.24539847992313923
2211	40	-0.3362192480562385
2211	41	-0.3228892840684985
2211	42	-0.40100043764547166
2211	43	-0.35412284324588583
2211	44	-0.48506249531357426
2211	45	-0.4630306378995108
2211	46	-0.4438205337472283
2211	47	-0.6177012261017296
2211	48	-0.4793231452043045
2211	49	-0.2789992988792953
2211	50	-0.1382745499990179
2212	1	-0.28673479003228053
2212	2	-0.3852257523244056
2212	3	-0.40889002476134095
2212	4	-0.3314854983466127
2212	5	-0.3271083711917342
2212	6	-0.46588260157466976
2212	7	-0.43005744147701463
2212	8	-0.32534601316199385
2212	9	-0.5065737222183841
2212	10	-0.5710564291226135
2212	11	-0.5568216074446397
2212	12	-0.5504022820602259
2212	13	-0.38080590225225563
2212	14	-1.1676294772000944
2212	15	-0.6680014711673081
2212	16	-0.6657156509216193
2212	17	-0.46357230440583663
2212	18	-0.36893579963257395
2212	19	-0.5169884262500162
2212	20	-0.29547410442258126
2212	21	-0.6056414980850775
2212	22	-0.475242147073331
2212	23	-0.6560822010006717
2212	24	-0.521996186977475
2212	25	-0.25332821911152337
2212	26	-0.4502646340987795
2212	27	-0.5796391221780937
2212	28	-0.5633992556587728
2212	29	-0.4165338950382278
2212	30	-0.6358139821333566
2212	31	-0.4111972406706812
2212	32	-0.36390373163251155
2212	33	-0.3115248447969847
2212	34	-0.26059847174963835
2212	35	-0.32244518117891374
2212	36	-0.3427102143448906
2212	37	-0.3500654591394614
2212	38	-0.38317071659054136
2212	39	-0.33535204174150834
2212	40	-0.37388541787371565
2212	41	-0.3384496004122113
2212	42	-0.3322606144450724
2212	43	-0.40458296166981
2212	44	-0.20930766989129168
2212	45	-0.3005739072327671
2212	46	-0.20823776900286006
2212	47	-0.17429775996372499
2212	48	-0.3053212299572685
2212	49	-0.22484724662978195
2212	50	-0.11222276289971234
2213	1	-0.8550928903747632
2213	2	-0.8982652918073732
2213	3	-0.8530080697916169
2213	4	-1.185292090433105
2213	5	-0.8915718039973868
2213	6	-0.9153387614520341
2213	7	-1.0906189461934872
2213	8	-1.1184059151940389
2213	9	-1.076812718150002
2213	10	-1.2122792051453004
2213	11	-1.1283077270011859
2213	12	-1.0601914867739737
2213	13	-1.0214123976413916
2213	14	-1.2205741262142666
2213	15	-1.366824312864992
2213	16	-1.2194686551814158
2213	17	-1.2197297065802104
2213	18	-1.1734683231943066
2213	19	-1.3157975306526382
2213	20	-1.2539336379815964
2213	21	-1.3763336597412774
2213	22	-1.0416309876043783
2213	23	-1.119676801459903
2213	24	-1.2372145934677512
2213	25	-1.2807797899897793
2213	26	-1.309466146603846
2213	27	-1.4486686173469359
2213	28	-1.3578794207373335
2213	29	-1.4804393791479349
2213	30	-1.5101415353519698
2213	31	-1.4404825960540926
2213	32	-1.577372014982167
2213	33	-1.7055365392366433
2213	34	-1.6127967689851026
2213	35	-1.5251418575785713
2213	36	-1.7481937052514631
2213	37	-1.461746904055385
2213	38	-1.4125401458698725
2213	39	-1.60320483239256
2213	40	-1.4098694166064192
2213	41	-1.3515313418556936
2213	42	-1.37357191970262
2213	43	-1.4800182441589236
2213	44	-1.383890084998523
2213	45	-1.5325867551101702
2213	46	-1.477256909633983
2213	47	-1.4448671935333834
2213	48	-1.6634125380035272
2213	49	-1.6903346648351203
2213	50	-1.523292960064964
2214	1	-0.707743362062299
2214	2	-0.9229813589020068
2214	3	-0.8747925846211331
2214	4	-0.8287139079437615
2214	5	-0.7445043049849218
2214	6	-0.7396322486903202
2214	7	-0.6913935554574344
2214	8	-0.800954646875379
2214	9	-1.0238835767076466
2214	10	-0.9631053123762356
2214	11	-1.2111569038756542
2214	12	-1.1871703020077788
2214	13	-1.0403576860928432
2214	14	-1.0910072290860882
2214	15	-0.8710615700238833
2214	16	-1.0240031704832404
2214	17	-0.8112863481422821
2214	18	-0.9861142098008671
2214	19	-1.0629032085550492
2214	20	-0.9895274523771533
2214	21	-1.0370150790004402
2214	22	-0.8739698052104004
2214	23	-0.9653178983735842
2214	24	-1.2389011015753866
2214	25	-1.2827914922138213
2214	26	-1.1823482264859422
2214	27	-1.3058479448579874
2214	28	-1.0388776689275545
2214	29	-1.069417918882607
2214	30	-1.049246025174643
2214	31	-1.0670882471376437
2214	32	-1.0818952852537969
2214	33	-1.1351288506259039
2214	34	-1.1514036518471258
2214	35	-1.2259608884081068
2214	36	-1.1384636081540336
2214	37	-1.1429553086353081
2214	38	-0.9588638793644648
2214	39	-0.9861173724247436
2214	40	-1.0602923127035737
2214	41	-0.9446981419595488
2214	42	-0.8075105707934824
2214	43	-0.9621993890278517
2214	44	-0.7146582125580707
2214	45	-0.7769292687087272
2214	46	-0.7277088259588034
2214	47	-0.6662789220588046
2214	48	-0.79213379900429
2214	49	-0.7967071470552014
2214	50	-0.7728271620008975
2215	1	-0.7486005893477738
2215	2	-0.8087441261623596
2215	3	-0.8602412577625387
2215	4	-0.8013443659053152
2215	5	-0.7428276515168726
2215	6	-0.8251020375478362
2215	7	-0.7401472490891123
2215	8	-0.7669348196841206
2215	9	-0.8873385740319257
2215	10	-0.8594390163136936
2215	11	-0.9822681525353083
2215	12	-0.8761949380746117
2215	13	-0.733308843956646
2215	14	-0.8035753706501296
2215	15	-0.8247965698585631
2215	16	-0.9157600041065095
2215	17	-0.8152266391817662
2215	18	-0.8104743565542307
2215	19	-1.0061850656399827
2215	20	-0.9546484935149024
2215	21	-0.9111389084313828
2215	22	-0.9329235724104024
2215	23	-1.0746372670188507
2215	24	-1.1838230092517072
2215	25	-1.1393528470520344
2215	26	-1.033217272874822
2215	27	-1.1893914900658658
2215	28	-1.1018659968399476
2215	29	-1.1746439377612283
2215	30	-1.1229545500795766
2215	31	-1.0421387681718244
2215	32	-1.1749429055100045
2215	33	-1.2425889830575265
2215	34	-1.1157893118414535
2215	35	-1.2371984031798284
2215	36	-1.343234171568131
2215	37	-1.2375480191896848
2215	38	-1.2172431540560176
2215	39	-1.2258343165092747
2215	40	-1.2454003978042252
2215	41	-1.3353671190964782
2215	42	-1.2698804395816659
2215	43	-1.2783643329853085
2215	44	-1.3215967971610887
2215	45	-1.3202003866477732
2215	46	-1.307979107125007
2215	47	-1.2988031764284251
2215	48	-1.5517353421608533
2215	49	-1.5232160639562267
2215	50	-1.639685752052909
2216	1	-0.501433625031865
2216	2	-0.5959049227780149
2216	3	-0.6236012533823434
2216	4	-0.5373394188621887
2216	5	-0.8107983654080471
2216	6	-0.704525913150313
2216	7	-0.757591937056624
2216	8	-0.6925029164091221
2216	9	-0.5591557266800891
2216	10	-0.7549323276601712
2216	11	-0.9641173192516312
2216	12	-0.9302750408803533
2216	13	-0.9587246000957634
2216	14	-1.011378960799199
2216	15	-1.193995026484174
2216	16	-1.1968360342976396
2216	17	-1.0431517475191185
2216	18	-1.0659117560070328
2216	19	-1.1370908801870598
2216	20	-1.159970510272366
2216	21	-1.2384598075951203
2216	22	-1.233764827400524
2216	23	-1.1070406410568552
2216	24	-1.3709716476070746
2216	25	-1.355426872337997
2216	26	-1.2399076978019963
2216	27	-1.2993077993655646
2216	28	-1.2565951575012946
2216	29	-1.5542352913862416
2216	30	-1.2891809159514196
2216	31	-1.4136883585753353
2216	32	-1.4132923129759796
2216	33	-1.4302722627925277
2216	34	-1.5614825500773293
2216	35	-1.5749858735692328
2216	36	-1.624561821384468
2216	37	-1.8695895440770833
2216	38	-1.7305391376431736
2216	39	-1.6132955268278515
2216	40	-1.8598434448091865
2216	41	-1.6454313404766587
2216	42	-1.6520367030181546
2216	43	-1.803182482449781
2216	44	-2.1673676550189462
2216	45	-2.151672808331668
2216	46	-2.185164977133077
2216	47	-2.04862643880368
2216	48	-2.140081206822508
2216	49	-2.245234753859396
2216	50	-2.431874915258632
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	193712.0
3	9683.0
4	16747.0
5	13642.0
6	11275.0
7	9419.0
8	8524.0
9	7734.0
10	7604.0
11	7872.0
12	8063.0
13	8340.0
14	8774.0
15	9236.0
16	9780.0
17	10442.0
18	11549.0
19	12786.0
20	15340.0
21	19240.0
22	23081.0
23	26706.0
24	29505.0
25	31985.0
26	36201.0
27	40715.0
28	46116.0
29	52662.0
30	61475.0
31	74156.0
32	89036.0
33	108998.0
34	136677.0
35	174892.0
36	225739.0
37	301342.0
38	453268.0
39	613979.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41886029941142	16.04345436405941	12.615590038042917	25.92209529848625
2	16.355738771997576	19.34507266993916	37.29612619678536	27.00306236127791
3	20.144026397085813	23.12929707770495	27.000639334353394	29.72603719085584
4	13.54624371478114	15.420067614741697	34.41513727338748	36.61855139708969
5	15.481802121047833	36.39281628017944	32.72454255179054	15.400839046982185
6	35.004325215149535	33.542436686030406	16.94239770780693	14.510840391013128
7	30.72859682713348	30.382692833698034	20.04991794310722	18.83879239606127
8	28.427073364126958	32.89396166559131	19.154963480863056	19.524001489418666
9	27.472950184024914	15.628098710115967	17.32843147311568	39.57051963274344
10	15.480044348551166	28.842421447303956	31.82442732693997	23.853106877204905
11	36.44851793085033	21.954090540939255	21.938261107697926	19.659130420512483
12	24.926334778258962	23.919263327267792	28.178101273353533	22.976300621119712
13	29.11453600204044	20.540900733094418	24.416057919997595	25.928505344867553
14	23.42082742553611	19.829342585681722	25.18432840183985	31.565501586942318
15	24.681802998387262	27.332854902440783	22.064564921351227	25.920777177820725
16	24.812283550394103	26.51919458608014	23.534775709571772	25.133746153953986
17	24.645885958661964	26.737165802095486	24.73211513817867	23.884833101063883
18	24.507569087692005	26.134457085708913	25.74283052252776	23.615143304071324
19	25.27357018927822	25.571230147739186	25.772086505694347	23.38311315728824
20	25.867207138384856	25.094901139018845	24.720665280835902	24.3172264417604
21	25.834406911299943	25.09819519203508	24.49112062388018	24.57627727278479
22	25.28347598760452	24.984122608687027	24.932001673068356	24.8003997306401
23	25.31107194206476	25.238242590126326	24.906886134898425	24.543799332910492
24	24.81061124212761	25.37888010987147	25.301147959881593	24.509360688119326
25	25.42472700335199	24.723754307975856	24.805151410581008	25.046367278091147
26	24.824281324983104	25.2194260449413	24.90444570299782	25.051846927077776
27	24.90390323842667	25.31349431079813	25.004377361381273	24.778225089393928
28	24.054436130036773	25.082630055757114	25.470956257976113	25.391977556229996
29	24.653357529277617	25.63905953200958	24.921868213273616	24.78571472543919
30	25.04959789877959	25.240431393135808	25.252640632828495	24.457330075256113
31	24.982100960838203	25.158321873617396	25.34886885594539	24.510708309599014
32	25.007812419867136	25.23794545324002	24.64909071638352	25.10515141050933
33	24.573141252310464	25.605883330404293	24.636292208054194	25.18468320923105
34	24.865398027980252	25.46432039166883	24.75684805054497	24.913433529805943
35	25.132286891254633	25.150340000218822	25.101582929417816	24.615790179108725
36	25.116574385022677	25.504369274281284	24.655268738234003	24.72378760246203
37	25.253073709534362	24.81681518626895	25.067204806605393	24.862906297591294
38	25.190611334079332	25.06072792828275	24.70321522114539	25.045445516492528
39	25.48221559152634	25.031651942487315	24.72684279609311	24.759289669893235
40	25.059689290871052	24.84896891301724	25.39838733776702	24.692954458344687
41	24.848792114626722	25.178386331873785	25.026460525272192	24.946361028227297
42	24.816012580336388	25.401955421851497	25.019964446875427	24.76206755093669
43	24.885801665131716	24.597100890408573	25.79942253007855	24.71767491438116
44	25.339059620411845	24.907434407564825	24.832773432999808	24.920732539023522
45	24.892640925556055	25.22113593643126	25.08734514844317	24.798877989569515
46	24.527741441066677	25.32297136044305	24.805260104957362	25.344027093532915
47	25.021039670803503	24.984839814782532	24.866771540281654	25.127348974132314
48	24.894696058455438	25.230048479292478	25.277218629201265	24.598036833050827
49	25.41312921324119	24.693447288383435	24.77958352446235	25.113839973913027
50	25.15663721351541	25.106150980157576	24.516791714378495	25.22042009194852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1515.0
1	1081.5
2	648.0
3	653.5
4	659.0
5	686.5
6	714.0
7	734.5
8	755.0
9	873.0
10	991.0
11	1165.5
12	1340.0
13	1816.5
14	2293.0
15	3030.5
16	3768.0
17	3851.5
18	3935.0
19	3924.5
20	3914.0
21	3894.0
22	3874.0
23	4388.5
24	4903.0
25	5869.0
26	6835.0
27	8737.5
28	10640.0
29	14676.5
30	18713.0
31	25006.0
32	31299.0
33	39948.0
34	48597.0
35	64980.5
36	81364.0
37	90513.5
38	99663.0
39	115776.5
40	131890.0
41	150866.0
42	169842.0
43	180235.5
44	190629.0
45	203054.0
46	215479.0
47	230774.0
48	246069.0
49	255672.0
50	265275.0
51	258152.5
52	251030.0
53	241914.0
54	232798.0
55	231297.5
56	229797.0
57	211621.5
58	193446.0
59	172092.0
60	150738.0
61	131445.0
62	112152.0
63	94973.5
64	77795.0
65	64622.5
66	51450.0
67	41504.5
68	31559.0
69	25464.5
70	19370.0
71	15406.5
72	11443.0
73	9487.0
74	7531.0
75	5958.5
76	4386.0
77	3591.5
78	2797.0
79	2315.0
80	1833.0
81	1457.0
82	1081.0
83	859.0
84	637.0
85	507.5
86	378.0
87	277.0
88	176.0
89	152.5
90	129.0
91	95.0
92	61.0
93	47.5
94	34.0
95	25.0
96	16.0
97	16.5
98	17.0
99	27.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08515887837692372
2	0.09342872130116751
3	0.10053668546745971
4	0.10142518098824624
5	0.09363375872904134
6	0.0541640538633323
7	0.051088492445225106
8	0.056863713330337504
9	0.05778638175576967
10	0.04387800956499601
11	0.04661184193664685
12	0.053890670626167224
13	0.0489697723571957
14	0.05084928211270566
15	0.04975574916404532
16	0.05255792734498744
17	0.05231871701246798
18	0.05365146029364777
19	0.06209216774111974
20	0.06615874339395038
21	0.0776408393548839
22	0.07948617620574822
23	0.05638529266529861
24	0.07350591789276201
25	0.08057970915440855
26	0.07870019939889861
27	0.07377930112992709
28	0.09301864644541989
29	0.08163906919842327
30	0.077914222592049
31	0.05320721253325451
32	0.05006330530585604
33	0.05320721253325451
34	0.046987743887748845
35	0.054369091291206116
36	0.05307052091467196
37	0.05648781137923552
38	0.04661184193664685
39	0.051737777633492174
40	0.05351476867506522
41	0.040426546195786825
42	0.037419330586970896
43	0.03830782610775742
44	0.03663335378012128
45	0.028431856665168752
46	0.02480952877273139
47	0.029696254137057267
48	0.024467799726275035
49	0.023818514538007957
50	0.025458813960998465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2926295.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.41947902571796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86826076479286	30.910336591526782
2	12.09968195896025	10.265244101519446
3	5.354953405349133	6.814630009857137
4	2.913017330656664	4.9427471023737315
5	1.7426186477455332	3.696048758893332
6	1.1300584599149048	2.8761894682917273
7	0.786553359943926	2.335562862832455
8	0.5197230433233025	1.7637104588348114
9	0.39916107328801304	1.5238984298601546
>10	1.881418059959379	13.94964506309285
>50	0.15998289754249798	4.751259486511504
>100	0.13019120970676384	10.892655649291068
>500	0.010660877916002346	2.963852035997151
>1k	0.0037189109009138875	2.3142199811178337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC	3465	0.11840911459712708	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.834580929127104E-5
2	0.0	0.0	0.0	0.0	6.834580929127104E-5
3	0.0	0.0	0.0	0.0	6.834580929127104E-5
4	0.0	0.0	0.0	0.0	6.834580929127104E-5
5	0.0	0.0	0.0	0.0	6.834580929127104E-5
6	0.0	0.0	0.0	0.0	1.0251871393690657E-4
7	0.0	0.0	0.0	3.417290464563552E-5	1.0251871393690657E-4
8	0.0	0.0	0.0	3.417290464563552E-5	1.0251871393690657E-4
9	0.0	0.0	0.0	3.417290464563552E-5	1.0251871393690657E-4
10	0.0	0.0	0.0	1.0251871393690657E-4	1.3669161858254208E-4
11	0.0	0.0	0.0	1.3669161858254208E-4	1.3669161858254208E-4
12	0.0	0.0	0.0	1.3669161858254208E-4	3.075561418107197E-4
13	0.0	0.0	0.0	1.7086452322817762E-4	4.442477603932618E-4
14	0.0	0.0	0.0	2.0503742787381313E-4	4.442477603932618E-4
15	0.0	0.0	0.0	2.0503742787381313E-4	5.125935696845328E-4
16	0.0	0.0	0.0	2.3921033251944864E-4	5.125935696845328E-4
17	0.0	0.0	0.0	2.3921033251944864E-4	5.125935696845328E-4
18	0.0	0.0	0.0	3.075561418107197E-4	6.151122836214394E-4
19	0.0	0.0	0.0	3.075561418107197E-4	6.492851882670749E-4
20	0.0	0.0	0.0	3.4172904645635524E-4	7.518039022039814E-4
21	0.0	0.0	0.0	4.442477603932618E-4	7.85976806849617E-4
22	0.0	0.0	0.0	5.467664743301683E-4	7.85976806849617E-4
23	0.0	0.0	0.0	6.151122836214394E-4	7.85976806849617E-4
24	0.0	0.0	0.0	7.85976806849617E-4	7.85976806849617E-4
25	0.0	0.0	0.0	9.568413300777946E-4	8.201497114952525E-4
26	0.0	0.0	0.0	0.0015377807090535985	8.884955207865236E-4
27	0.0	0.0	0.0	0.002152892992675038	9.226684254321591E-4
28	0.0	0.0	0.0	0.003212253036689739	9.568413300777946E-4
29	0.0	0.0	0.0	0.00526262731542787	9.568413300777946E-4
30	0.0	0.0	0.0	0.008816609398573965	9.568413300777946E-4
31	0.0	0.0	0.0	0.018726751745808266	9.568413300777946E-4
32	0.0	0.0	0.0	0.03096065160894578	9.568413300777946E-4
33	0.0	0.0	0.0	0.04647515031806431	9.568413300777946E-4
34	0.0	0.0	0.0	0.06680802858221745	9.568413300777946E-4
35	0.0	0.0	0.0	0.09189094059211392	9.568413300777946E-4
36	0.0	0.0	0.0	0.12339835867538987	9.568413300777946E-4
37	0.0	0.0	0.0	0.16003171245551115	9.568413300777946E-4
38	3.417290464563552E-5	0.0	0.0	0.2025086329300361	9.568413300777946E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1880	0.0	22.826523	1
GTATCAA	3860	0.0	22.23516	1
CGATCGT	180	0.0	20.78129	25
AACTCGA	190	1.8189894E-12	18.524378	37
AAACTCG	190	1.8189894E-12	18.523111	36
CCGCGAT	205	0.0	18.246674	22
ACTCGAG	220	1.8189894E-12	16.999968	38
GATCGTT	210	9.094947E-12	16.76474	26
TATCAAC	5130	0.0	16.643614	2
TCAACGC	5000	0.0	16.635952	4
ATCAACG	5060	0.0	16.526228	3
GCGCGAC	80	0.001991333	16.497427	11
ATCGTTC	215	1.2732926E-11	16.374863	27
CAACGCA	5170	0.0	16.255844	5
TATAGGC	810	0.0	16.02521	5
CGCGATC	220	1.8189894E-11	16.002432	23
AACGCAG	5400	0.0	15.765288	6
GTATTAA	420	0.0	15.719363	1
TATCATA	575	0.0	15.308245	2
GTACATG	9135	0.0	15.249589	1
>>END_MODULE