FastQCFastQC Report
Mon 6 Feb 2017
SRR2077146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926295
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC32600.1114036691447718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.023.4562071
GTATCAA44700.022.5437531
TCGACCG602.8710937E-422.00343718
GTATTTA3900.016.9248521
GGGTCGA1206.4022315E-616.502866
TATAGGC8050.016.1283975
TATCAAC62350.015.95062352
ATCAACG62200.015.8124893
TCAACGC62600.015.6768384
CGACCGT850.00297853115.53157219
ACCGTAC1158.206956E-515.3080478
CCTAACG1158.215341E-515.3062142
CAACGCA64150.015.2307545
AACGCAG66950.014.6253866
ATATCGA10400.014.59169834
GTATAAA3550.014.2550171
AATATCG10700.014.18258433
GTCGTGT3650.013.85830736
TCGCACG2104.01742E-813.62024422
TATCGAA11150.013.60972835
Produced by FastQC (version 0.11.5)