FastQCFastQC Report
Mon 6 Feb 2017
SRR2077145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267150
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31850.021.9731791
GGTATCA16900.021.356611
TATACCG1203.1555464E-718.3385565
TATCAAC41150.017.0071852
ATCAACG42200.016.3754183
CTATAGA3100.016.3283141
CAACGCA42200.016.3221865
TCAACGC42500.016.2080444
AACGCAG44200.015.6769716
GTATTAC2700.015.48691751
GTATAAA3500.015.0910121
ATTATAC1809.023461E-814.6718183
GCACCGT4200.014.665026
GTATTAG1851.2616692E-714.2752831
ACCGTGC4800.014.206118
TATTACA3750.014.0849462
TCGATCG1100.001020027713.99719141
CGATACT1901.7563434E-713.89378915
TGCGATA1901.7582352E-713.89256313
GTACATG70600.013.8093811
Produced by FastQC (version 0.11.5)