FastQCFastQC Report
Mon 6 Feb 2017
SRR2077145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267150
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG454.0678817E-529.3367311
GTATCAA36650.023.5934541
GGTATCA18350.021.7027851
GCGAAAT550.00448007920.00364111
TACCGTA808.9849986E-519.252237
TATGCGA750.001289577717.60359212
TATCAAC49700.017.486552
GCGATAC1307.3115007E-716.92578514
ATCAACG51000.016.825483
TCAACGC50350.016.8241944
CAACGCA50900.016.5559465
AACGCAG53250.015.8655766
TGCGATA1552.6640555E-715.6160913
GTACTTT27400.013.89273414
TACTTTT28100.013.54665115
GAGTACT28300.013.45150912
CGATACT1658.708161E-613.33546615
ACTTTTT29400.013.32185716
AGTACTT27950.013.3050413
GCGGTAT1000.00875944413.20094724
Produced by FastQC (version 0.11.5)