FastQCFastQC Report
Mon 6 Feb 2017
SRR2077144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431429
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60500.019.896511
GGTATCA34750.016.5283931
TCGCGCA800.00199181916.49694311
GCGTAAA1259.4251045E-615.844461
TATCAAC75700.015.6105082
ATCAACG77250.015.3831953
TCAACGC76750.015.3400474
CAACGCA76950.015.2697945
AACGCAG79600.014.78455456
TACCGTA2002.1231244E-814.2981847
TAGATCG1100.00101728914.0025115
GTACATG114650.013.9734051
TAGCGCG1150.001423812413.39253429
TACATGG115850.013.2965992
ACATGGG119550.012.8670453
ACGCAGA90800.012.6459717
AGAGTAC81300.012.58069411
GTCCTAA5250.012.5749681
GCACCGT4450.012.3579826
GTCTTAG7400.012.192621
Produced by FastQC (version 0.11.5)