FastQCFastQC Report
Mon 6 Feb 2017
SRR2077144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431429
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67200.020.889211
GGTATCA36150.019.9634741
CGTACCG600.00740448418.33776310
CGCGTAA600.00740448418.33776310
CTATACT6250.015.4901614
TATCAAC89850.015.4521252
CAACGCA91250.015.1435975
TCAACGC91800.015.0522094
ATCAACG92050.015.0352313
AACGCAG94700.014.6142866
CCTATAC4550.014.0239343
CGTACAC2855.456968E-1213.8966763
GCACCGT4700.013.5761646
TATACCG1952.404522E-713.5409435
TATTCCG2309.140422E-913.3937585
ACCGTGC5050.013.071518
TAGGGTG5400.013.0394275
CTAGTAC3400.012.9429823
ATACCGT2054.4202352E-712.8796576
GTACATG106800.012.85535051
Produced by FastQC (version 0.11.5)