FastQCFastQC Report
Mon 6 Feb 2017
SRR2077143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524712
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG40310.11436395370742349No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC37170.10545542444318853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26050.024.5820731
GTATCAA50350.021.9400441
CGCGTAA600.00741914118.33149310
ATCAACG67800.016.1960933
GTCTACG1501.8351784E-716.1374421
TATCAAC69100.016.0187782
TCAACGC68250.015.9916684
CGTATAC850.002976120715.5335863
CAACGCA71200.015.3902475
GTACATG112850.015.0733981
AACGCAG74550.014.9025236
TACATGG113350.014.7547372
GTATAGC7950.014.6704011
TTACGCT1057.152137E-414.6697764
AATATCG13350.014.6645733
CGATCGG900.004369058614.66269843
ATATCGA13500.014.17575334
CGACGGT1402.7607251E-514.14224157
TATACCG1251.67005E-414.0823865
GTGTATA7900.013.9275961
Produced by FastQC (version 0.11.5)