##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077143_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3524712
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.118661326088485	31.0	30.0	34.0	23.0	34.0
2	29.161068195075227	31.0	30.0	34.0	22.0	34.0
3	29.216488042143585	31.0	30.0	34.0	22.0	34.0
4	32.57849577497395	35.0	33.0	37.0	24.0	37.0
5	32.55642702155524	37.0	33.0	37.0	24.0	37.0
6	32.62825586884829	37.0	33.0	37.0	25.0	37.0
7	32.44076849399327	37.0	33.0	37.0	22.0	37.0
8	32.38337061297491	37.0	33.0	37.0	22.0	37.0
9	33.69676728197935	38.0	34.0	39.0	19.0	39.0
10	33.65106936396506	38.0	34.0	39.0	19.0	39.0
11	33.72135028337067	38.0	34.0	39.0	22.0	39.0
12	33.62692441254775	38.0	34.0	39.0	19.0	39.0
13	33.56913103822383	38.0	34.0	39.0	18.0	39.0
14	34.51851867613581	39.0	34.0	41.0	17.0	41.0
15	34.5851090812526	39.0	34.0	41.0	18.0	41.0
16	34.570253399426676	39.0	34.0	41.0	18.0	41.0
17	34.42300165233358	39.0	34.0	41.0	17.0	41.0
18	34.39488048952652	39.0	34.0	41.0	17.0	41.0
19	34.52556974867734	39.0	34.0	41.0	18.0	41.0
20	34.510338433324485	39.0	34.0	41.0	18.0	41.0
21	34.47798203087231	39.0	34.0	41.0	18.0	41.0
22	34.410366293756766	39.0	34.0	41.0	18.0	41.0
23	34.37562444818187	39.0	34.0	41.0	18.0	41.0
24	34.37412702087433	39.0	34.0	41.0	18.0	41.0
25	34.28467403861649	39.0	34.0	41.0	18.0	41.0
26	34.245380615494255	39.0	34.0	41.0	17.0	41.0
27	34.19241657190715	39.0	34.0	41.0	17.0	41.0
28	34.09155045859066	39.0	34.0	41.0	16.0	41.0
29	34.08194031171909	39.0	34.0	41.0	16.0	41.0
30	33.92139925191051	39.0	33.0	41.0	15.0	41.0
31	33.92111298738734	39.0	33.0	41.0	15.0	41.0
32	33.79620178896886	39.0	33.0	41.0	10.0	41.0
33	33.75316706726677	39.0	33.0	41.0	10.0	41.0
34	33.686679649287655	39.0	33.0	41.0	9.0	41.0
35	33.61321917932586	38.0	33.0	41.0	9.0	41.0
36	33.527570479517195	38.0	33.0	40.0	9.0	41.0
37	33.44386605203489	38.0	33.0	40.0	8.0	41.0
38	33.35478728474837	38.0	33.0	40.0	8.0	41.0
39	33.252755402427205	38.0	33.0	40.0	7.0	41.0
40	33.19853083032032	38.0	33.0	40.0	2.0	41.0
41	33.09017758046615	38.0	33.0	40.0	2.0	41.0
42	32.94922336917172	38.0	32.0	40.0	2.0	41.0
43	32.868681753289344	38.0	32.0	40.0	2.0	41.0
44	32.730462800932386	38.0	32.0	40.0	2.0	41.0
45	32.60164263066032	38.0	32.0	40.0	2.0	41.0
46	32.468991509093506	38.0	32.0	40.0	2.0	41.0
47	32.32204929083568	38.0	32.0	40.0	2.0	41.0
48	32.152274568815834	38.0	31.0	40.0	2.0	41.0
49	31.98957900673871	38.0	31.0	40.0	2.0	41.0
50	31.7957373538604	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.6899663404056966
1101	2	1.6531085965520234
1101	3	1.75488848402982
1101	4	1.804997299216673
1101	5	1.96630988526163
1101	6	2.0458716795569245
1101	7	2.015900115686158
1101	8	2.0329635114106637
1101	9	2.25295236782042
1101	10	2.330952171758078
1101	11	2.2933873778477647
1101	12	2.2699763741783627
1101	13	2.4159520244550023
1101	14	2.790984989578753
1101	15	2.818133006617934
1101	16	2.8602476056017636
1101	17	2.913548859163953
1101	18	2.8188517374652733
1101	19	2.7571367067886996
1101	20	2.827938795140234
1101	21	2.898629881895424
1101	22	2.934557735730934
1101	23	2.9119596613114282
1101	24	2.8080666953201927
1101	25	2.9060488917187683
1101	26	2.978875933057928
1101	27	2.987513898733276
1101	28	2.926382376386208
1101	29	2.9212190314522815
1101	30	3.095667342330195
1101	31	3.074606209199473
1101	32	3.1389573151092094
1101	33	3.205914503535496
1101	34	3.175545190204261
1101	35	3.1729767955138826
1101	36	3.1174872698163867
1101	37	3.129136935664583
1101	38	3.1342986813969205
1101	39	3.232252482111903
1101	40	3.2249184472476884
1101	41	3.295370314161616
1101	42	3.3005972232536536
1101	43	3.287877526142694
1101	44	3.3574443020097675
1101	45	3.40859313640771
1101	46	3.390309905641942
1101	47	3.407641709597904
1101	48	3.3515124638122415
1101	49	3.392741555498773
1101	50	3.2843123480315555
1102	1	0.7163092894526812
1102	2	0.6717960174191511
1102	3	0.6192883574172221
1102	4	0.8315659266676505
1102	5	0.7107021447832338
1102	6	0.844299202398517
1102	7	0.7585039722375058
1102	8	0.9062448309192703
1102	9	0.9290568916543833
1102	10	0.9671426723911409
1102	11	0.9816217309258164
1102	12	0.8963088862407744
1102	13	1.0743438733411566
1102	14	1.2365220665386758
1102	15	1.1189085943399348
1102	16	1.2321994928138906
1102	17	1.4630359070372094
1102	18	1.4462804751034355
1102	19	1.3758332814045815
1102	20	1.2746296721891284
1102	21	1.2104707264356804
1102	22	1.3383373784780872
1102	23	1.2218154256066782
1102	24	1.0874397918537397
1102	25	1.255912697625078
1102	26	1.2508761554854715
1102	27	1.1284473244595858
1102	28	1.2695407619044659
1102	29	1.196342478736959
1102	30	1.448691758030158
1102	31	1.3977912860225175
1102	32	1.4205220073037168
1102	33	1.421144288294073
1102	34	1.4068728259706091
1102	35	1.3896479962804023
1102	36	1.414457977820355
1102	37	1.475242742734352
1102	38	1.4201039546406022
1102	39	1.568523398855561
1102	40	1.5984294659658218
1102	41	1.4927342056169763
1102	42	1.48602325754171
1102	43	1.33410909032159
1102	44	1.2230759106741758
1102	45	1.3079245705520464
1102	46	1.3052335958859231
1102	47	1.2175946576182994
1102	48	1.2061087578272307
1102	49	1.271979912272549
1102	50	1.2130116602171626
1103	1	0.18980692704685964
1103	2	0.19407849399609844
1103	3	0.1743043669141393
1103	4	0.18173756171733402
1103	5	0.2128413162622138
1103	6	0.12985262966054023
1103	7	0.10242769419723885
1103	8	0.27412935444164077
1103	9	0.3132537919130911
1103	10	0.25502757427680933
1103	11	0.15142247535560216
1103	12	0.1706498956194551
1103	13	0.1642768557425356
1103	14	0.27141699978120215
1103	15	0.22428769071379406
1103	16	0.187244257921229
1103	17	0.42202964985421687
1103	18	0.20850846736590256
1103	19	0.34085794596187213
1103	20	0.24684721216557648
1103	21	0.15423458990328953
1103	22	0.13223362180352183
1103	23	0.22110334588553116
1103	24	0.06812302137364412
1103	25	0.207273717692523
1103	26	0.17419515830900423
1103	27	0.22611783624324033
1103	28	0.16197340141410876
1103	29	0.2404848000903641
1103	30	0.2858561143159548
1103	31	0.2519369653501684
1103	32	0.4475297684807984
1103	33	0.3579470238606959
1103	34	0.3473629010284327
1103	35	0.3346279193780646
1103	36	0.34674892027602766
1103	37	0.3352398104188197
1103	38	0.23804688799663154
1103	39	0.39466414054221133
1103	40	0.290063380559765
1103	41	0.2459109911370163
1103	42	0.3143346694516467
1103	43	0.26039067774479463
1103	44	0.2567674594570235
1103	45	0.2801385160771943
1103	46	0.32954551858630765
1103	47	0.34799321605414235
1103	48	0.31425955451844345
1103	49	0.3158677843100861
1103	50	0.35964578764045996
1104	1	0.5065672397357872
1104	2	0.5903179966927361
1104	3	0.5409311512267045
1104	4	0.5498671920516003
1104	5	0.6378874346381096
1104	6	0.531033880162596
1104	7	0.6052844263527035
1104	8	0.6550664347432829
1104	9	0.674734484203114
1104	10	0.5447941568100276
1104	11	0.6084046354008095
1104	12	0.6559248513331326
1104	13	0.6218432993216823
1104	14	0.7647264882583045
1104	15	0.728280294862401
1104	16	0.831118793390452
1104	17	0.865232808874886
1104	18	0.6814362408454073
1104	19	0.7996637019861481
1104	20	0.7612365682276234
1104	21	0.7404505155050813
1104	22	0.7219527119221354
1104	23	0.6883621880007311
1104	24	0.7485498048406498
1104	25	0.7374525400215646
1104	26	0.7817976087484908
1104	27	0.8119208455993885
1104	28	0.8131561316501461
1104	29	0.8401792702539908
1104	30	0.7880259680477764
1104	31	0.7949937011697372
1104	32	0.9027238461626084
1104	33	0.9035224078516677
1104	34	0.9206668087945005
1104	35	0.9765204568681796
1104	36	0.9658107125580173
1104	37	0.9469333730122713
1104	38	0.9045444422077438
1104	39	0.9963656936991896
1104	40	0.8783672812794805
1104	41	0.9756174260068775
1104	42	0.9143989321847528
1104	43	0.8577017897014727
1104	44	0.9820676692974004
1104	45	0.9447696324871586
1104	46	0.9383774165776728
1104	47	0.8748816737062697
1104	48	0.785952243364207
1104	49	0.8601886357349287
1104	50	0.9458450838507915
1105	1	0.4268133649954571
1105	2	0.4028194675762897
1105	3	0.37853855857836294
1105	4	0.4227237488592479
1105	5	0.41215014417704765
1105	6	0.30042651665372233
1105	7	0.44960850025753984
1105	8	0.42249991407189214
1105	9	0.39078637231585134
1105	10	0.347043733583817
1105	11	0.3959133009208742
1105	12	0.4380467380923605
1105	13	0.4843210329819172
1105	14	0.5233253183130486
1105	15	0.5364695831506836
1105	16	0.4513420953158658
1105	17	0.545230638239012
1105	18	0.39544642312267086
1105	19	0.41448367957735144
1105	20	0.4492634506629045
1105	21	0.49907165520479424
1105	22	0.4561680855910737
1105	23	0.36933354815079866
1105	24	0.4992814536793091
1105	25	0.4441880159648406
1105	26	0.5973858743650311
1105	27	0.6353742317017463
1105	28	0.5884395895770211
1105	29	0.5185817360103329
1105	30	0.5722794264011313
1105	31	0.4826668547763333
1105	32	0.5859687293693767
1105	33	0.5657621182073669
1105	34	0.5052476604411495
1105	35	0.5317841652341144
1105	36	0.4657567751834293
1105	37	0.5352789697812383
1105	38	0.5474279521725407
1105	39	0.5733982098723374
1105	40	0.5921434960082905
1105	41	0.6560112739684669
1105	42	0.650658371075977
1105	43	0.6708391302651577
1105	44	0.6370748137596038
1105	45	0.7512261751504283
1105	46	0.7165188983132111
1105	47	0.5294784640561758
1105	48	0.685241906657609
1105	49	0.7060653056282788
1105	50	0.7114079278210141
1106	1	0.37628082542498476
1106	2	0.3209657751013246
1106	3	0.3657085256040453
1106	4	0.366346762549405
1106	5	0.4011078105479484
1106	6	0.22110678379229398
1106	7	0.2721474923126479
1106	8	0.27216520635979435
1106	9	0.3533613642426374
1106	10	0.3593420700211496
1106	11	0.2858083268593745
1106	12	0.4163731082032669
1106	13	0.46831206123220426
1106	14	0.4929347807082465
1106	15	0.3919397464392418
1106	16	0.32456472898526556
1106	17	0.49257985994347564
1106	18	0.17248748330285224
1106	19	0.29256973830915456
1106	20	0.30006982390239756
1106	21	0.47156716078251293
1106	22	0.4694123758540556
1106	23	0.3810463275783462
1106	24	0.3589951196970773
1106	25	0.36950654797189486
1106	26	0.4125383123827504
1106	27	0.42756981406576955
1106	28	0.4353355754028172
1106	29	0.32950214592131033
1106	30	0.3352150727373413
1106	31	0.3216517909387946
1106	32	0.38220899906515626
1106	33	0.25807216578490255
1106	34	0.4072652737524649
1106	35	0.3482393827098207
1106	36	0.3697783493470723
1106	37	0.4320441332021048
1106	38	0.3942712484053743
1106	39	0.3839309414670993
1106	40	0.4684337177799591
1106	41	0.4608302290144053
1106	42	0.4064440981911943
1106	43	0.3713882494942169
1106	44	0.4776959959595146
1106	45	0.41917782390146385
1106	46	0.4494107631103077
1106	47	0.37185139959010627
1106	48	0.4605885866161046
1106	49	0.35439657938396607
1106	50	0.402489078458693
1107	1	0.10798802741298275
1107	2	0.0388288342694203
1107	3	0.1407770204519423
1107	4	0.05424099908203317
1107	5	-0.0651669838980311
1107	6	-0.05341474228733034
1107	7	-0.027553036859011115
1107	8	0.03453429932144303
1107	9	0.04944672946579942
1107	10	0.004525210333419238
1107	11	0.04427849791252925
1107	12	-0.03548744535865467
1107	13	0.15767114352195222
1107	14	-0.01639582041548948
1107	15	0.059210669579520925
1107	16	-0.019828778902166277
1107	17	-0.003736155597330537
1107	18	0.1285628372172667
1107	19	0.010828977131261297
1107	20	0.06277639037029559
1107	21	0.011480914851340174
1107	22	-0.19400472605732233
1107	23	-0.2871226722524298
1107	24	-0.25675823504952433
1107	25	-0.21070475015940104
1107	26	0.07173470858949571
1107	27	-0.013007493159655326
1107	28	-0.1428377393877085
1107	29	-0.048145384104365974
1107	30	-0.04253310880498873
1107	31	-0.08166818645644724
1107	32	0.015107704270384659
1107	33	-0.053594025269333656
1107	34	-0.040773561695885974
1107	35	0.07291096552425103
1107	36	-0.020540358455349406
1107	37	-0.004380871980998791
1107	38	-0.01542445400934156
1107	39	0.08564677047982627
1107	40	0.1520362685910257
1107	41	0.19760992585794668
1107	42	-0.04307750170534064
1107	43	-0.04271973519610128
1107	44	0.10543327865341467
1107	45	0.23565577693504025
1107	46	0.08159147591757687
1107	47	0.0785570485283884
1107	48	0.02596743493000986
1107	49	0.15584289275657426
1107	50	0.1573324369667688
1108	1	0.07198542392812612
1108	2	0.04166725369991653
1108	3	0.06318215124269955
1108	4	-0.010517476447404306
1108	5	0.019904300753495363
1108	6	-0.11705530249029295
1108	7	-0.12331188994454578
1108	8	-0.08015786062388486
1108	9	-0.09373711883786484
1108	10	-0.023491500982608216
1108	11	0.05430486520221933
1108	12	-0.010716500861455813
1108	13	-0.09930513350782633
1108	14	-0.11444982991372399
1108	15	-0.03898727408520841
1108	16	0.01661421633359339
1108	17	-0.15766407305856234
1108	18	0.007183302994960172
1108	19	-0.1280159865668793
1108	20	-0.15942175087575094
1108	21	-0.06415032592823167
1108	22	-0.27418587308422104
1108	23	-0.24206214670837767
1108	24	-0.16733511057606165
1108	25	-0.13220161755097593
1108	26	-0.1987693825907968
1108	27	-0.11224913074580911
1108	28	-0.11457299560236578
1108	29	-0.27256337600073266
1108	30	-0.20070514374903325
1108	31	-0.10550869530081997
1108	32	0.06293040180383258
1108	33	-0.030012849038989486
1108	34	-0.0853323436932314
1108	35	-0.039717006117747644
1108	36	-0.17972718063749937
1108	37	-0.11227142916630584
1108	38	0.004473959401835259
1108	39	0.017015683992887887
1108	40	-0.04091427310110163
1108	41	0.06913701114912385
1108	42	0.08492531556912297
1108	43	0.13993603223367757
1108	44	0.03372504291105116
1108	45	-0.029247152110983166
1108	46	-0.04939061309622872
1108	47	-0.0056862437014331135
1108	48	-0.03196581026409717
1108	49	-0.05699884280982559
1108	50	-0.05587272951646227
1109	1	-0.1384096006880391
1109	2	-0.1586906505729857
1109	3	-0.20204727339551098
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2210	36	0.03893383026982633
2210	37	0.23857821369925603
2210	38	0.16530268041495333
2210	39	0.27983865136437913
2210	40	0.002144072138385411
2210	41	0.06628178938269258
2210	42	0.16449958160433908
2210	43	0.07736219431055247
2210	44	-0.06775761771441324
2210	45	0.22344716926233588
2210	46	0.1750099276927557
2210	47	0.2804059418766016
2210	48	0.2825801168997373
2210	49	0.378121690323745
2210	50	0.400347959550551
2211	1	-0.21031516757237512
2211	2	-0.1651782918076634
2211	3	-0.28872926403810695
2211	4	-0.20697598497353908
2211	5	-0.5057807231974465
2211	6	-0.2629182945210644
2211	7	-0.2553953068448038
2211	8	-0.24197064053556971
2211	9	-0.2008404279249021
2211	10	-0.22628307185502194
2211	11	-0.23338531818400554
2211	12	-0.3114955645047246
2211	13	-0.40339717499296057
2211	14	-0.5765852144925745
2211	15	-0.39199790447320026
2211	16	-0.3888625261602172
2211	17	-0.29298675917095096
2211	18	-0.5177805861064115
2211	19	-0.391277779673338
2211	20	-0.4261854916202168
2211	21	-0.4121840483577941
2211	22	-0.45628141111544096
2211	23	-0.5307982330440311
2211	24	-0.43736675999261365
2211	25	-0.37905890805044606
2211	26	-0.4551822092089992
2211	27	-0.39458480417809483
2211	28	-0.2663566473827714
2211	29	-0.3543281057630949
2211	30	-0.743842711369318
2211	31	-0.5078983965960404
2211	32	-0.2847935755824693
2211	33	-0.3920566407988275
2211	34	-0.3717766824386288
2211	35	-0.2575603011770795
2211	36	-0.33774140600917235
2211	37	-0.207886798180823
2211	38	-0.3677195106718898
2211	39	-0.2805664788338049
2211	40	-0.3258691629977122
2211	41	-0.3520344929933401
2211	42	-0.3986247047246607
2211	43	-0.4146140225793786
2211	44	-0.40613655028747786
2211	45	-0.31868949090493004
2211	46	-0.39924682359547603
2211	47	-0.365393443765754
2211	48	-0.40403692963907645
2211	49	-0.34479733849546434
2211	50	-0.3104956742870648
2212	1	-0.30542497851503114
2212	2	-0.4103131396638311
2212	3	-0.3743326488677816
2212	4	-0.33873070480544953
2212	5	-0.395450630910652
2212	6	-0.4127863563069951
2212	7	-0.3198108781130742
2212	8	-0.1848176181351704
2212	9	-0.3496850546699406
2212	10	-0.2157421469950691
2212	11	-0.29823110715307877
2212	12	-0.3591714387929841
2212	13	-0.31529902297251766
2212	14	-1.0217768673007512
2212	15	-0.7234917629480151
2212	16	-0.7045672092130459
2212	17	-0.4910933395958921
2212	18	-0.3522979189785005
2212	19	-0.368847875078238
2212	20	-0.35000866589546575
2212	21	-0.5015502756376762
2212	22	-0.5425140758997031
2212	23	-0.4622861908755951
2212	24	-0.5180603366891958
2212	25	-0.35811232832480044
2212	26	-0.5450787342790875
2212	27	-0.6708676162658875
2212	28	-0.5562826981906284
2212	29	-0.5697626176175987
2212	30	-0.5678465675072459
2212	31	-0.419790053579149
2212	32	-0.36982012749176363
2212	33	-0.3889405324960151
2212	34	-0.28998397017783617
2212	35	-0.3764513090612809
2212	36	-0.576601049271936
2212	37	-0.5043053301306273
2212	38	-0.5849593209493165
2212	39	-0.4669053861636172
2212	40	-0.46742066788554837
2212	41	-0.4820513382600353
2212	42	-0.4775785769809744
2212	43	-0.4415300488531173
2212	44	-0.436788562076039
2212	45	-0.44341582957184755
2212	46	-0.36327229646967396
2212	47	-0.2919770267855384
2212	48	-0.3200666379139179
2212	49	-0.30030981034670745
2212	50	-0.29998461861835324
2213	1	-0.5545758946342758
2213	2	-0.5384744370558963
2213	3	-0.5598290696583206
2213	4	-0.7118322136535653
2213	5	-0.4984254171433591
2213	6	-0.6563250867235553
2213	7	-0.701356264183346
2213	8	-0.6358749904595129
2213	9	-0.653057264829485
2213	10	-0.8323223490956337
2213	11	-0.6307585428479356
2213	12	-0.8195463579914346
2213	13	-0.7166673546575879
2213	14	-0.7378287055245352
2213	15	-0.9575225218656485
2213	16	-0.7429891526123669
2213	17	-0.7255447269884527
2213	18	-0.6564417724436211
2213	19	-0.7041130791217256
2213	20	-0.8262433412251653
2213	21	-0.8802985928475309
2213	22	-0.8195115547071339
2213	23	-0.8349744377719901
2213	24	-0.8807558948598668
2213	25	-0.9352665299133918
2213	26	-0.88634176151497
2213	27	-0.9409678193977626
2213	28	-1.0441664249883829
2213	29	-0.9409370439956461
2213	30	-1.1190923386625187
2213	31	-1.0639932443886693
2213	32	-1.0757194234363112
2213	33	-1.125887882113048
2213	34	-1.211991274085591
2213	35	-1.1871574455592722
2213	36	-1.3822714305079273
2213	37	-1.1964181778995808
2213	38	-1.0819296541187455
2213	39	-1.2610567350660915
2213	40	-1.1001689151134286
2213	41	-1.1665641440398105
2213	42	-1.1085573853353452
2213	43	-1.150598791406022
2213	44	-1.080859155467902
2213	45	-1.054914603966548
2213	46	-1.193608375674529
2213	47	-1.2273530970032382
2213	48	-1.32061618054491
2213	49	-1.3333659782445046
2213	50	-1.2383949597436263
2214	1	-0.384414517168576
2214	2	-0.5815553133865734
2214	3	-0.5263059531188325
2214	4	-0.5465653781115734
2214	5	-0.34473359116648083
2214	6	-0.37736342811273715
2214	7	-0.3479390132072311
2214	8	-0.4956381503002696
2214	9	-0.6081103073461165
2214	10	-0.4585779090576736
2214	11	-0.6650500556119212
2214	12	-0.6142584757066913
2214	13	-0.5082888890428592
2214	14	-0.6167835144382607
2214	15	-0.5598525931122893
2214	16	-0.5463919423402288
2214	17	-0.42988563166009897
2214	18	-0.5381342794760968
2214	19	-0.6393714196414138
2214	20	-0.5163904433629511
2214	21	-0.6539726347741066
2214	22	-0.5721562157666469
2214	23	-0.6686438302919271
2214	24	-0.8285272620356423
2214	25	-0.8383079491929948
2214	26	-0.7535684351860326
2214	27	-0.9126040904170694
2214	28	-0.6614633360126376
2214	29	-0.6220970919211624
2214	30	-0.5554389562611206
2214	31	-0.6294014632703195
2214	32	-0.7070008438006781
2214	33	-0.7165171889474635
2214	34	-0.749799211654377
2214	35	-0.6964760390900011
2214	36	-0.6983676455470373
2214	37	-0.6176361630793537
2214	38	-0.4969615799977518
2214	39	-0.49190055763880736
2214	40	-0.5442532021738913
2214	41	-0.5414340592056135
2214	42	-0.35814499862269855
2214	43	-0.5153386475987318
2214	44	-0.45485790099480283
2214	45	-0.35907260774673944
2214	46	-0.425989242317776
2214	47	-0.3455828948351254
2214	48	-0.36157468521968994
2214	49	-0.4520882553186638
2214	50	-0.48439973412229165
2215	1	-0.38086392088498044
2215	2	-0.4834324448053131
2215	3	-0.5054438430325838
2215	4	-0.4682226714955746
2215	5	-0.46131972662471554
2215	6	-0.41706568305057345
2215	7	-0.36598614398723583
2215	8	-0.2891751286002986
2215	9	-0.40201413417343446
2215	10	-0.39413441171144825
2215	11	-0.5296679908369981
2215	12	-0.41767036186833906
2215	13	-0.4458268666197043
2215	14	-0.43571505334490723
2215	15	-0.4844757946568663
2215	16	-0.6057178425540428
2215	17	-0.4662105418528455
2215	18	-0.49658078915248893
2215	19	-0.520009391065912
2215	20	-0.5423027240400771
2215	21	-0.5228706509694874
2215	22	-0.5129173424999749
2215	23	-0.6178797826969813
2215	24	-0.6504179750453858
2215	25	-0.6848719622694475
2215	26	-0.6703332495465517
2215	27	-0.6388173433459485
2215	28	-0.6106897461149643
2215	29	-0.6898903100490017
2215	30	-0.5631455835794981
2215	31	-0.6585919117863455
2215	32	-0.6225869115458167
2215	33	-0.6420513795196285
2215	34	-0.5583548288225799
2215	35	-0.6128723142449601
2215	36	-0.5481760988638413
2215	37	-0.5966440427003121
2215	38	-0.5779934106839448
2215	39	-0.6566719405422745
2215	40	-0.5226649027907655
2215	41	-0.602851213807277
2215	42	-0.48482625995006146
2215	43	-0.6508117224441676
2215	44	-0.6804898328294087
2215	45	-0.6870577430742131
2215	46	-0.6443655280965075
2215	47	-0.5766193806176574
2215	48	-0.7264523688024873
2215	49	-0.6634495731215608
2215	50	-0.7635112867073879
2216	1	-0.15887640181939844
2216	2	-0.17193808878877803
2216	3	-0.16880342863268183
2216	4	-0.12050367040194487
2216	5	-0.14154671891850512
2216	6	-0.15073196189965898
2216	7	-0.21618438478069635
2216	8	-0.05172100342090857
2216	9	-0.03363277905884132
2216	10	-0.1765848173965594
2216	11	-0.21682504045288908
2216	12	-0.24083756678263768
2216	13	-0.22604323518731206
2216	14	-0.21327953674460076
2216	15	-0.40789586646976517
2216	16	-0.40343927940865143
2216	17	-0.2824128588565884
2216	18	-0.28707011845658315
2216	19	-0.3897111349120834
2216	20	-0.46242606875404135
2216	21	-0.44434128092868264
2216	22	-0.276724854228128
2216	23	-0.567395542308482
2216	24	-0.4265046973394391
2216	25	-0.5694592602599684
2216	26	-0.5290650638536576
2216	27	-0.45933214737747363
2216	28	-0.588583866521212
2216	29	-0.7000910310342476
2216	30	-0.6392811242359144
2216	31	-0.6920433993619
2216	32	-0.6545902321148063
2216	33	-0.7539450813240904
2216	34	-0.7106458246632243
2216	35	-0.6426026413890824
2216	36	-0.8086556232382733
2216	37	-0.8980348304777763
2216	38	-0.8455637764067134
2216	39	-0.7679495217665746
2216	40	-0.89661157501763
2216	41	-0.8407892778803259
2216	42	-0.8264497493815526
2216	43	-0.9168287153282577
2216	44	-0.9924398824332421
2216	45	-1.0587361029803546
2216	46	-1.2795304664043137
2216	47	-0.9978025193757389
2216	48	-1.1316404127229873
2216	49	-1.1406743749759762
2216	50	-1.1943156416258454
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	184286.0
3	8764.0
4	16314.0
5	12293.0
6	10371.0
7	8782.0
8	8231.0
9	7306.0
10	7289.0
11	7519.0
12	7612.0
13	7749.0
14	8209.0
15	8753.0
16	9276.0
17	10261.0
18	11153.0
19	12604.0
20	15859.0
21	20756.0
22	25766.0
23	28862.0
24	30385.0
25	32685.0
26	36053.0
27	40737.0
28	46882.0
29	53427.0
30	62227.0
31	75286.0
32	92255.0
33	115068.0
34	148229.0
35	192737.0
36	256811.0
37	353821.0
38	564493.0
39	985601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84186692464455	16.743186411070184	13.053883169981734	25.361063494303544
2	15.942657028097852	18.965538784486206	38.8226811548108	26.26912303260514
3	19.64522345849902	23.49487922397992	27.6126579244371	29.24723939308396
4	13.204727506642111	15.713634536983523	35.469449134431194	35.61218882194318
5	14.908246915319198	36.882669814699064	33.16858605200898	15.040497217972751
6	33.9514897610597	34.91229260457737	16.893676659156284	14.242540975206655
7	30.170388821471406	31.132627933547653	20.259621184702617	18.43736206027833
8	27.717999175615475	34.14768746515386	19.000984492259757	19.13332886697091
9	26.869513402322916	16.309128500542645	17.90603484226391	38.91532325487053
10	15.260794879130959	29.417635117644654	32.12146396181601	23.200106041408375
11	35.86239588078927	22.65066328319148	22.09085709500838	19.39608374101087
12	24.17350949536516	24.528836966093294	28.79688281545852	22.500770723083026
13	28.64452567155477	21.283388973935445	24.682314917765357	25.38977043674443
14	22.536252350707876	20.649923712876557	25.90988894014122	30.90393499627435
15	24.19940230516727	28.071417946968207	22.670647892569352	25.058531855295175
16	23.936330568264978	27.284900069774874	24.272601183504516	24.50616817845563
17	23.503518353088854	27.40276023734442	25.506974114152527	23.586747295414202
18	23.451131364680347	26.87044695653803	26.40436632308395	23.274055355697673
19	24.285170832801033	26.309074649746627	26.640236199236327	22.765518318216014
20	24.620993380905414	26.00715909278032	25.72878462930061	23.643062897013657
21	24.92638962475603	25.69046106075035	25.555847063052507	23.827302251441115
22	24.327452336095657	25.813058397777272	25.913234586340206	23.946254679786872
23	24.189824507515894	25.986792145917182	25.711058391228526	24.112324955338398
24	23.788523741164074	25.958806172884373	26.206933364830732	24.04573672112082
25	24.210216963532748	25.634577870912235	25.856396889081974	24.298808276473043
26	23.85170247817492	25.76909513309138	26.14733212450445	24.231870264229247
27	23.98519501109579	26.057365357052813	25.82741444201909	24.130025189832303
28	23.258585069752584	25.834198289020627	26.310432714493626	24.596783926733163
29	23.768072402907997	26.277288735643804	25.852356379820524	24.102282481627675
30	24.14636313998097	25.90601000669252	26.088783628344594	23.85884322498192
31	24.012185923906674	25.80694700990958	26.41201355871388	23.768853507469867
32	24.02769782416412	26.025894751901575	25.65968827741879	24.286719146515516
33	23.563389201570477	26.292447980315398	25.67049123228339	24.473671585830743
34	23.994713522839234	26.127442590033183	25.730985188269724	24.146858698857862
35	24.178175656552494	25.93257805631563	26.066396588280348	23.822849698851527
36	24.217281853474752	26.25760632964717	25.653698671823282	23.87141314505479
37	24.12523281656879	25.685973208032763	26.017373985237708	24.171419990160743
38	24.208771832220457	25.819414340716047	25.788418065945994	24.183395761117502
39	24.328916785794984	25.932436928226654	25.549532589306494	24.189113696671864
40	24.134425709231987	25.704406170347394	26.191387959130434	23.969780161290185
41	24.012710018293003	26.046426478164058	25.937803789447393	24.003059714095546
42	24.064645423394772	26.027497180944348	25.88096021307037	24.026897182590513
43	23.98319319144878	25.335463512684292	26.790761250565513	23.89058204530141
44	24.181199562012225	25.561292461285817	25.983329672082476	24.274178304619475
45	23.884358313051692	26.016882196955937	26.079287978132154	24.01947151186022
46	23.854398216926427	26.03809009754518	25.794376132831488	24.313135552696902
47	24.093009712705527	25.901087287208195	25.656056024011587	24.349846976074687
48	24.037595457215584	26.000346214204427	26.095838902557727	23.866219426022255
49	24.449050549033917	25.48664434623049	25.673685363579246	24.390619741156343
50	24.230528449262753	25.940878294968307	25.421521535668212	24.40707172010073
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2760.0
1	2123.5
2	1487.0
3	1403.5
4	1320.0
5	1293.5
6	1267.0
7	1185.5
8	1104.0
9	1289.0
10	1474.0
11	1752.0
12	2030.0
13	3077.5
14	4125.0
15	5575.5
16	7026.0
17	7735.0
18	8444.0
19	8647.0
20	8850.0
21	9653.5
22	10457.0
23	12747.0
24	15037.0
25	19244.0
26	23451.0
27	28774.5
28	34098.0
29	42458.0
30	50818.0
31	59740.5
32	68663.0
33	79561.5
34	90460.0
35	109841.0
36	129222.0
37	137380.0
38	145538.0
39	161371.5
40	177205.0
41	194478.5
42	211752.0
43	216009.0
44	220266.0
45	231454.0
46	242642.0
47	259223.0
48	275804.0
49	279457.5
50	283111.0
51	274837.5
52	266564.0
53	258960.0
54	251356.0
55	250090.5
56	248825.0
57	230131.0
58	211437.0
59	188826.0
60	166215.0
61	146108.0
62	126001.0
63	106887.5
64	87774.0
65	72916.0
66	58058.0
67	46717.0
68	35376.0
69	28700.5
70	22025.0
71	17524.5
72	13024.0
73	10752.0
74	8480.0
75	6537.0
76	4594.0
77	3646.5
78	2699.0
79	2184.0
80	1669.0
81	1325.5
82	982.0
83	749.5
84	517.0
85	398.5
86	280.0
87	226.0
88	172.0
89	128.5
90	85.0
91	67.5
92	50.0
93	39.5
94	29.0
95	24.5
96	20.0
97	22.0
98	24.0
99	34.5
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08406360576410214
2	0.09373815506061205
3	0.10117138648490998
4	0.10261831321254047
5	0.09529856623746848
6	0.05767847131907514
7	0.05353628892232898
8	0.05912539804670566
9	0.061026262571239866
10	0.044258935198109806
11	0.04842948870716245
12	0.0580472957790594
13	0.051209857713197554
14	0.05211773330700495
15	0.05231633109315031
16	0.05543715344686318
17	0.053876742270006744
18	0.054784617863814125
19	0.06417545603725922
20	0.07030361629545903
21	0.07915540333508099
22	0.08275853459800403
23	0.059891418078980636
24	0.0773963943720792
25	0.08290039015953643
26	0.08074418562424392
27	0.0753536742860126
28	0.09467440176672591
29	0.08440405911177991
30	0.08139672120729297
31	0.05557900900839558
32	0.05208936219469846
33	0.05557900900839558
34	0.049961528771712414
35	0.057621729094462185
36	0.05640177126528352
37	0.05926725360823807
38	0.048457859819468936
39	0.05390511338231322
40	0.05654362682681592
41	0.04275526624586633
42	0.038840052747572004
43	0.03909539275833033
44	0.03762009491839333
45	0.02837111230648064
46	0.025789341086590905
47	0.03064080129099909
48	0.025023321054315928
49	0.025136805503541852
50	0.026385134445026995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3524712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.92646758134819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35463756508236	37.621908694973264
2	12.655026262867358	12.636415169083143
3	4.843662119851222	7.254808194052689
4	2.3989921423599863	4.790928136937796
5	1.3111267742656438	3.2729964195205645
6	0.8021291990113665	2.4028486470296264
7	0.5189868818796376	1.8137827213316096
8	0.362924338404797	1.449562417267947
9	0.2523696278403274	1.1339931638598313
>10	1.3101791822475384	11.659350960796361
>50	0.10212128635853165	3.568446445235652
>100	0.07775866274443984	7.5624883497042825
>500	0.006973718903099832	2.357074680487204
>1k	0.0031122381838124776	2.4753959997200767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG	4031	0.11436395370742349	No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC	3717	0.10545542444318853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.511333691944193E-5
2	2.8371112306480643E-5	0.0	0.0	0.0	1.1348444922592257E-4
3	2.8371112306480643E-5	0.0	0.0	0.0	1.1348444922592257E-4
4	2.8371112306480643E-5	0.0	0.0	0.0	1.1348444922592257E-4
5	2.8371112306480643E-5	0.0	0.0	0.0	1.1348444922592257E-4
6	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
7	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
8	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
9	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
10	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
11	2.8371112306480643E-5	0.0	0.0	0.0	1.418555615324032E-4
12	2.8371112306480643E-5	0.0	0.0	0.0	2.5534001075832577E-4
13	2.8371112306480643E-5	0.0	0.0	0.0	2.837111230648064E-4
14	2.8371112306480643E-5	0.0	0.0	2.8371112306480643E-5	3.1208223537128706E-4
15	2.8371112306480643E-5	0.0	0.0	2.8371112306480643E-5	4.2556668459720965E-4
16	2.8371112306480643E-5	0.0	0.0	2.8371112306480643E-5	4.539377969036903E-4
17	2.8371112306480643E-5	0.0	0.0	2.8371112306480643E-5	4.8230890921017094E-4
18	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	5.390511338231322E-4
19	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	5.390511338231322E-4
20	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	5.390511338231322E-4
21	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	5.674222461296128E-4
22	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	6.525355830490548E-4
23	2.8371112306480643E-5	0.0	0.0	5.674222461296129E-5	7.660200322749774E-4
24	2.8371112306480643E-5	0.0	0.0	1.985977861453645E-4	7.660200322749774E-4
25	2.8371112306480643E-5	0.0	0.0	2.837111230648064E-4	7.660200322749774E-4
26	2.8371112306480643E-5	0.0	0.0	4.539377969036903E-4	7.660200322749774E-4
27	2.8371112306480643E-5	0.0	0.0	8.227622568879386E-4	8.511333691944193E-4
28	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.0014752978399369934	8.511333691944193E-4
29	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.0029789667921804675	8.511333691944193E-4
30	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.005191913552085958	8.511333691944193E-4
31	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.01100799157491449	8.511333691944193E-4
32	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.01688081182235598	9.646178184203419E-4
33	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.025307032177380735	9.646178184203419E-4
34	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.03713778600918316	9.646178184203419E-4
35	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.051521939948568844	9.646178184203419E-4
36	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.06967945182471647	9.646178184203419E-4
37	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.09376652617291853	9.646178184203419E-4
38	2.8371112306480643E-5	2.8371112306480643E-5	0.0	0.11921541391183166	9.646178184203419E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2605	0.0	24.582073	1
GTATCAA	5035	0.0	21.940044	1
CGCGTAA	60	0.007419141	18.331493	10
ATCAACG	6780	0.0	16.196093	3
GTCTACG	150	1.8351784E-7	16.137442	1
TATCAAC	6910	0.0	16.018778	2
TCAACGC	6825	0.0	15.991668	4
CGTATAC	85	0.0029761207	15.533586	3
CAACGCA	7120	0.0	15.390247	5
GTACATG	11285	0.0	15.073398	1
AACGCAG	7455	0.0	14.902523	6
TACATGG	11335	0.0	14.754737	2
GTATAGC	795	0.0	14.670401	1
TTACGCT	105	7.152137E-4	14.669776	4
AATATCG	1335	0.0	14.66457	33
CGATCGG	90	0.0043690586	14.662698	43
ATATCGA	1350	0.0	14.175753	34
CGACGGT	140	2.7607251E-5	14.1422415	7
TATACCG	125	1.67005E-4	14.082386	5
GTGTATA	790	0.0	13.927596	1
>>END_MODULE