FastQCFastQC Report
Mon 6 Feb 2017
SRR2077143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524712
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC39110.1109594202306458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32700.025.1656171
GTATCAA61250.021.9851321
CGCAATA951.6014767E-518.52234836
GTTACGC850.002988254415.52410542
TTACGCG850.002988254415.52410543
TATCAAC87350.015.3658592
ATCAACG88200.015.0928263
TCAACGC88200.015.0678794
TTAATAC7000.014.7735073
ATTGCGA1951.5133992E-814.67060310
CAACGCA90400.014.628375
TAAGGCG2900.014.4160245
AACGCAG93850.014.0896216
GTACATG101800.014.00591
ATATCGC1100.001018033414.0011746
TACATGG102600.013.6609852
CGTAAAC1453.8348135E-513.6570943
CGCACGA2104.0268787E-813.61806428
TATACTG6850.013.491145
ATACCGT1801.4463731E-613.4455726
Produced by FastQC (version 0.11.5)