FastQCFastQC Report
Mon 6 Feb 2017
SRR2077142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013125
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.021.3394471
GTATCAA44450.020.5953271
TATCAAC57200.015.9647872
TAGATCG850.00297859915.531545
TCAACGC58650.015.1578924
ATCAACG59250.015.1160663
TATATAC4150.014.8471353
CAACGCA60000.014.8153645
GGTTACG900.00435271714.670871
AACGCAG60950.014.6532356
GTCCTAA3800.014.4778331
GGACCGT2907.2759576E-1213.6538686
TACACCG2309.145879E-913.3931415
GTATAGC6150.013.2395661
ACCGTGC4000.013.1983018
GTCTAGG6050.013.0946611
GTACATG93500.012.921351
GTCTTAG6850.012.8503981
CGCTTCG1557.1620554E-512.77743122
CTAGGAC7750.012.777433
Produced by FastQC (version 0.11.5)