##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077142_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3013125
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.378602281684298	31.0	30.0	34.0	16.0	34.0
2	28.517293507571043	31.0	30.0	34.0	16.0	34.0
3	28.586750300767477	31.0	30.0	34.0	16.0	34.0
4	31.94072034847542	35.0	33.0	37.0	17.0	37.0
5	31.864987678904793	35.0	33.0	37.0	17.0	37.0
6	31.955702136486206	35.0	33.0	37.0	17.0	37.0
7	31.783872889442023	35.0	32.0	37.0	17.0	37.0
8	31.716226509023024	35.0	32.0	37.0	17.0	37.0
9	32.9814040240614	38.0	33.0	39.0	16.0	39.0
10	32.926580543455714	38.0	33.0	39.0	15.0	39.0
11	32.94534046878241	38.0	33.0	39.0	15.0	39.0
12	32.83771333748185	38.0	33.0	39.0	12.0	39.0
13	32.77304227338726	38.0	33.0	39.0	11.0	39.0
14	33.64593735739473	39.0	33.0	41.0	10.0	41.0
15	33.68378411118025	39.0	33.0	41.0	10.0	41.0
16	33.6423009334163	39.0	33.0	41.0	10.0	41.0
17	33.485523169466916	38.0	33.0	41.0	10.0	41.0
18	33.46112258867455	38.0	33.0	41.0	10.0	41.0
19	33.58907712092927	39.0	33.0	41.0	10.0	41.0
20	33.563931466500726	39.0	33.0	41.0	10.0	41.0
21	33.50601883426675	39.0	33.0	41.0	10.0	41.0
22	33.42769948143539	39.0	33.0	41.0	10.0	41.0
23	33.379653681808755	39.0	32.0	41.0	9.0	41.0
24	33.38018336444721	39.0	32.0	41.0	8.0	41.0
25	33.28312101223813	38.0	32.0	41.0	8.0	41.0
26	33.23088487865588	38.0	32.0	41.0	8.0	41.0
27	33.163406430201206	38.0	32.0	41.0	7.0	41.0
28	33.05717685127567	38.0	32.0	40.0	2.0	41.0
29	33.03767317983821	38.0	32.0	40.0	2.0	41.0
30	32.88037834474176	38.0	32.0	40.0	2.0	41.0
31	32.86643634100809	38.0	32.0	40.0	2.0	41.0
32	32.73849176519394	38.0	32.0	40.0	2.0	41.0
33	32.688543289773904	38.0	32.0	40.0	2.0	41.0
34	32.60168164281269	38.0	31.0	40.0	2.0	41.0
35	32.54163932794026	38.0	31.0	40.0	2.0	41.0
36	32.44244264675378	38.0	31.0	40.0	2.0	41.0
37	32.34741074465879	38.0	31.0	40.0	2.0	41.0
38	32.261576602364656	38.0	31.0	40.0	2.0	41.0
39	32.15297871810828	38.0	31.0	40.0	2.0	41.0
40	32.08582186268409	38.0	31.0	40.0	2.0	41.0
41	31.971087492221532	38.0	31.0	40.0	2.0	41.0
42	31.825447002696535	38.0	30.0	40.0	2.0	41.0
43	31.745061356565028	38.0	30.0	40.0	2.0	41.0
44	31.600707239162	38.0	30.0	40.0	2.0	41.0
45	31.456884795685543	38.0	30.0	40.0	2.0	41.0
46	31.320717527483925	38.0	30.0	40.0	2.0	41.0
47	31.15503372744244	37.0	30.0	40.0	2.0	41.0
48	30.986122713130055	37.0	30.0	40.0	2.0	41.0
49	30.808604687824104	37.0	30.0	40.0	2.0	41.0
50	30.597331673926572	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.39655815202212
1101	2	1.737446364552902
1101	3	1.776738300268896
1101	4	1.9847466154628108
1101	5	1.9427392988643852
1101	6	1.9606608970902428
1101	7	1.8920337237464189
1101	8	2.132857668743565
1101	9	2.216825692935359
1101	10	2.2535979287283325
1101	11	2.1959040652906268
1101	12	2.3841637430570373
1101	13	2.302025983579732
1101	14	2.6813559872187014
1101	15	2.7720851263087596
1101	16	2.676727653882189
1101	17	2.97489468611122
1101	18	2.783684768995826
1101	19	2.691257773606644
1101	20	2.779557204820321
1101	21	2.830684251044069
1101	22	2.8207762865686377
1101	23	2.760873175287635
1101	24	2.7685634954303353
1101	25	2.8952453799443205
1101	26	2.8960755602589714
1101	27	2.8950339514559644
1101	28	3.0388399058244033
1101	29	2.895858842272993
1101	30	3.0373578968969994
1101	31	2.963523032856642
1101	32	3.0196214099477885
1101	33	3.160415219527522
1101	34	3.260072872029099
1101	35	3.2417422609453013
1101	36	3.3134111003020976
1101	37	3.1842414890768467
1101	38	3.208444274277909
1101	39	3.257215399095898
1101	40	3.154503794622677
1101	41	3.1124127611230463
1101	42	3.328832125020355
1101	43	3.266407898435368
1101	44	3.161173920494086
1101	45	3.2320342845713412
1101	46	3.165501863692967
1101	47	3.2787590495755445
1101	48	3.1259247167517366
1101	49	3.3463207926781244
1101	50	3.517529533666398
1102	1	0.8969151803927282
1102	2	0.999855997930279
1102	3	0.9509215024656825
1102	4	1.1410851835161466
1102	5	1.0869766404701444
1102	6	1.1460507760285097
1102	7	1.285848030548845
1102	8	1.1930291045516732
1102	9	1.4477243924248953
1102	10	1.46384961818206
1102	11	1.289737227035502
1102	12	1.4571954086184107
1102	13	1.3830856991895288
1102	14	1.615072808032302
1102	15	1.4336750926525497
1102	16	1.397889909849205
1102	17	1.7692429524459072
1102	18	1.6631974464711519
1102	19	1.6269132330312033
1102	20	1.6354050920773773
1102	21	1.4481200127922236
1102	22	1.5455065647793873
1102	23	1.4800003978104428
1102	24	1.5043723950762455
1102	25	1.5849420726121508
1102	26	1.5882968929726644
1102	27	1.4729756758323234
1102	28	1.6263026694331728
1102	29	1.549777786938968
1102	30	1.6276934244141898
1102	31	1.6712551705548293
1102	32	1.6978382129523695
1102	33	1.682287094115864
1102	34	1.6195605829599344
1102	35	1.6480306528024542
1102	36	1.6619028745154338
1102	37	1.6227110557297735
1102	38	1.6374985250639114
1102	39	1.73090540461952
1102	40	1.64937281920308
1102	41	1.6200320518493
1102	42	1.6372335425049656
1102	43	1.605841385152388
1102	44	1.5687384838311473
1102	45	1.5370132614518468
1102	46	1.6297523839706543
1102	47	1.603656986952256
1102	48	1.4963304852899348
1102	49	1.5794365733321065
1102	50	1.6553214248538737
1103	1	0.3788748783579834
1103	2	0.5679300754193903
1103	3	0.4820951443817201
1103	4	0.5419286678601694
1103	5	0.5419881361514989
1103	6	0.46903131455157165
1103	7	0.4587823658539172
1103	8	0.5708100672222791
1103	9	0.6931085807015052
1103	10	0.6357847212022065
1103	11	0.6303571719925358
1103	12	0.5923423268337089
1103	13	0.6018413994238543
1103	14	0.6514210099153033
1103	15	0.6506387895514223
1103	16	0.5218498363249466
1103	17	0.7237152435442198
1103	18	0.6585300194391621
1103	19	0.6702229870216172
1103	20	0.5868145407857881
1103	21	0.5169748344638379
1103	22	0.5051373800206349
1103	23	0.5975580215418645
1103	24	0.5329482334992832
1103	25	0.6727712830683146
1103	26	0.6551151649923597
1103	27	0.5468697552056412
1103	28	0.6215821237340862
1103	29	0.6518257621155144
1103	30	0.7451628511134345
1103	31	0.6389309582645666
1103	32	0.7687877141380213
1103	33	0.835427209132817
1103	34	0.7289389341965986
1103	35	0.5664263487872816
1103	36	0.6401613038465612
1103	37	0.7332650333156963
1103	38	0.5914145735161469
1103	39	0.6415157080373532
1103	40	0.5483260807894652
1103	41	0.6406440991629978
1103	42	0.656948585911934
1103	43	0.7256460287261319
1103	44	0.7756858041447288
1103	45	0.6034917744498856
1103	46	0.6246479807338261
1103	47	0.6152849638526519
1103	48	0.48858518228301406
1103	49	0.5522799053358014
1103	50	0.5654907431108604
1104	1	0.3775586989694055
1104	2	0.4255062951165023
1104	3	0.3408601712685204
1104	4	0.36959148332298497
1104	5	0.4370949641678372
1104	6	0.30855187519719607
1104	7	0.36103503417151117
1104	8	0.3904311046038593
1104	9	0.44721611749647394
1104	10	0.260469106756986
1104	11	0.4787227354022292
1104	12	0.4445117266593712
1104	13	0.46666310433973024
1104	14	0.5280389401084093
1104	15	0.6132546159370449
1104	16	0.5598769362766021
1104	17	0.5736846568869325
1104	18	0.46700815561130327
1104	19	0.5933187726481393
1104	20	0.5554532525762994
1104	21	0.5086124596915056
1104	22	0.528916123246951
1104	23	0.55056461197006
1104	24	0.6307579515648172
1104	25	0.5991128935727943
1104	26	0.45311412175339427
1104	27	0.6138354721551593
1104	28	0.5496159648810206
1104	29	0.5352117484009042
1104	30	0.5794672169471866
1104	31	0.5798371897977646
1104	32	0.5408633957525808
1104	33	0.5872541770332873
1104	34	0.5697080889824093
1104	35	0.6477088741880692
1104	36	0.6821343425313131
1104	37	0.6591474107221273
1104	38	0.7285667044443755
1104	39	0.7020149030797143
1104	40	0.6742850548107313
1104	41	0.7006805951971273
1104	42	0.703411711227119
1104	43	0.702971177890241
1104	44	0.8667673240849751
1104	45	0.7320874396360324
1104	46	0.699897222999212
1104	47	0.7536995525617591
1104	48	0.690670273843697
1104	49	0.9031651854419955
1104	50	0.9928478652963264
1105	1	0.4068985979999802
1105	2	0.39742538939119854
1105	3	0.364760170782489
1105	4	0.45993388680074077
1105	5	0.40430815219648153
1105	6	0.48499411078604027
1105	7	0.441843943879487
1105	8	0.4592848287800031
1105	9	0.5072839239978961
1105	10	0.3354526771807613
1105	11	0.3662978271578936
1105	12	0.410526935604004
1105	13	0.4962155550457936
1105	14	0.6162210669513328
1105	15	0.5893542258626283
1105	16	0.45294660601174286
1105	17	0.5102056086324112
1105	18	0.3649220333870602
1105	19	0.34502625953572874
1105	20	0.43972919342773764
1105	21	0.5405579176644224
1105	22	0.5453890082840047
1105	23	0.5175116113548839
1105	24	0.45369390034550605
1105	25	0.39045167750989407
1105	26	0.5462148801002797
1105	27	0.5380398831622273
1105	28	0.6143673722872975
1105	29	0.5070079029253662
1105	30	0.523479942621691
1105	31	0.3812924874638455
1105	32	0.6532068504454855
1105	33	0.6272322049703405
1105	34	0.5200500637115866
1105	35	0.514203726080602
1105	36	0.5117455335289165
1105	37	0.5667202823586166
1105	38	0.6103458357407305
1105	39	0.7478957314566941
1105	40	0.6318281397036429
1105	41	0.5984600333872052
1105	42	0.5323423289444662
1105	43	0.5127174663508924
1105	44	0.5604492450591465
1105	45	0.6639667974227308
1105	46	0.8047570621369076
1105	47	0.575066012480189
1105	48	0.6682682038386005
1105	49	0.5426257294631931
1105	50	0.5643439912838346
1106	1	0.1936883869956887
1106	2	0.11470427110887726
1106	3	0.08954909069921868
1106	4	0.10535997971808087
1106	5	0.1759340288546376
1106	6	0.10245152423183868
1106	7	-0.006752518834328924
1106	8	0.13051208282728055
1106	9	0.11390589316917499
1106	10	0.16417434790088237
1106	11	0.18227801687311995
1106	12	0.1810089460060027
1106	13	0.1563607468158139
1106	14	0.2839272037268472
1106	15	0.14562860554908497
1106	16	0.14991923002391871
1106	17	0.18300600510758613
1106	18	-0.15576276210989448
1106	19	0.06986638100467246
1106	20	-0.027695461130207377
1106	21	0.09985929437983998
1106	22	0.14020375635215743
1106	23	0.004562039831029097
1106	24	0.022035930082829225
1106	25	-0.059601587262768874
1106	26	0.055045295169705355
1106	27	0.17518260917329087
1106	28	-0.027434189696897704
1106	29	0.007923972802011292
1106	30	0.11111005182921474
1106	31	0.03613802383379294
1106	32	0.17247753991480863
1106	33	0.06215730585760326
1106	34	0.18329349677566142
1106	35	0.11426204363802128
1106	36	0.146603221846199
1106	37	0.18069708502379456
1106	38	0.18836255877401697
1106	39	0.10878924064939355
1106	40	0.18949064568277407
1106	41	0.23591047399003884
1106	42	0.1642340747152815
1106	43	0.1633058631359745
1106	44	0.15859238876708304
1106	45	0.21780106102988128
1106	46	0.11395422516669385
1106	47	0.11619866997548911
1106	48	0.18790732552987066
1106	49	0.1559111006407612
1106	50	0.2269983117516574
1107	1	0.09635703387904826
1107	2	-0.10234489080563591
1107	3	-0.034478187728595344
1107	4	-0.11414262194885794
1107	5	-0.13856132663149268
1107	6	-0.12340587167309636
1107	7	0.006571121265348978
1107	8	0.037103598715091834
1107	9	0.02309418258116125
1107	10	-0.10483365727581884
1107	11	-0.11319053404144341
1107	12	-0.20897486855123049
1107	13	-0.060670906162016536
1107	14	-0.22787945631753104
1107	15	-0.1903635947151585
1107	16	-0.18274181244743204
1107	17	-0.11383716251508957
1107	18	-0.0653105628491133
1107	19	-0.07737948183800114
1107	20	-0.04188125118277952
1107	21	-0.0722009025007182
1107	22	-0.32039388890233056
1107	23	-0.35775885231536364
1107	24	-0.29201488836704925
1107	25	-0.2595494733993462
1107	26	-0.02978595729119604
1107	27	-0.06610627155760795
1107	28	-0.10233795764897735
1107	29	-0.07267416385895586
1107	30	-0.11448937632231093
1107	31	-0.04391126547475466
1107	32	-0.028570064365055714
1107	33	-0.0386379260955394
1107	34	-0.061107267092992856
1107	35	-0.016165618334611054
1107	36	-0.056943039840959386
1107	37	-0.10249332404319489
1107	38	-0.0889030573702172
1107	39	-0.006075619472163396
1107	40	0.06664530307025274
1107	41	0.10331170386895394
1107	42	-0.021241064762598683
1107	43	-0.09519421976305864
1107	44	0.07964880532781393
1107	45	0.11537192318602862
1107	46	-0.10929194408236853
1107	47	0.11844113529984668
1107	48	-0.061923934157601224
1107	49	0.0015507869582478406
1107	50	0.0790658425641837
1108	1	-0.2119835269166721
1108	2	-0.16682545668642845
1108	3	-0.16029798875295143
1108	4	-0.32300179336863266
1108	5	-0.29678661845936816
1108	6	-0.33008886658370784
1108	7	-0.4265807744709633
1108	8	-0.22977799082350003
1108	9	-0.38496561643717797
1108	10	-0.37257142939049004
1108	11	-0.34006658259337996
1108	12	-0.5182651557617532
1108	13	-0.39154608815118763
1108	14	-0.5997415754673057
1108	15	-0.6154320897283085
1108	16	-0.4614340239906767
1108	17	-0.594066236640316
1108	18	-0.41801846158827516
1108	19	-0.4191212176387751
1108	20	-0.6092643627016088
1108	21	-0.5264373678104022
1108	22	-0.6360679121528179
1108	23	-0.8908285125151352
1108	24	-0.7050886045755576
1108	25	-0.5246064288966537
1108	26	-0.7254135603276168
1108	27	-0.6269626039340892
1108	28	-0.8279023571272504
1108	29	-0.7579136788280465
1108	30	-0.703980788485012
1108	31	-0.6083432276377749
1108	32	-0.49465492743323836
1108	33	-0.5230460244030368
1108	34	-0.6929690719875765
1108	35	-0.673323419463344
1108	36	-0.7824973033680394
1108	37	-0.7083893457922592
1108	38	-0.7394223396536859
1108	39	-0.7261933902695716
1108	40	-0.6889922600647118
1108	41	-0.6793573420188999
1108	42	-0.6193325262128901
1108	43	-0.7076782841345697
1108	44	-0.720031216126209
1108	45	-0.6237963068188144
1108	46	-0.6134876986900935
1108	47	-0.6054811586718856
1108	48	-0.6673243526706187
1108	49	-0.6000198702690369
1108	50	-0.6529036232288519
1109	1	-0.4364103206581227
1109	2	-0.39284341505633336
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2210	36	-0.1423354530310803
2210	37	-0.01934586422009943
2210	38	0.027122825181571386
2210	39	0.07452034863597845
2210	40	-0.1502216555063356
2210	41	-0.058109536527233274
2210	42	-0.08129648393661526
2210	43	-0.13513082920990982
2210	44	-0.1837284066333531
2210	45	0.04559109627556168
2210	46	0.06507175104575325
2210	47	-0.006610047580053902
2210	48	0.03960550155208864
2210	49	0.1875621669657619
2210	50	0.09919713170691935
2211	1	-0.15288509969161623
2211	2	-0.28154750764926106
2211	3	-0.3571498254257435
2211	4	-0.4282266896131617
2211	5	-0.5604890128042079
2211	6	-0.28599711748485035
2211	7	-0.32450776372300183
2211	8	-0.2434849586849417
2211	9	-0.28774587896099035
2211	10	-0.2990103547289138
2211	11	-0.31459649042216853
2211	12	-0.43441616338071753
2211	13	-0.41322206222113067
2211	14	-0.644482011570922
2211	15	-0.24606332609437942
2211	16	-0.3992933964725225
2211	17	-0.15565709182456544
2211	18	-0.3802001121644736
2211	19	-0.38926581599888266
2211	20	-0.458034522307436
2211	21	-0.44115894360268015
2211	22	-0.40482239904979167
2211	23	-0.5115165278029536
2211	24	-0.3487646190349949
2211	25	-0.3326105581287919
2211	26	-0.4748746971177411
2211	27	-0.28698500030632346
2211	28	-0.2512517235071101
2211	29	-0.27348525555086667
2211	30	-0.6089501221652185
2211	31	-0.4775906247920574
2211	32	-0.22713140946050459
2211	33	-0.4791509563170351
2211	34	-0.29953612706657395
2211	35	-0.12151557368469668
2211	36	-0.22356566100832964
2211	37	-0.2089355543469935
2211	38	-0.22004733940620014
2211	39	-0.18099152395801354
2211	40	-0.28212584753509873
2211	41	-0.288458085700416
2211	42	-0.3076218290545292
2211	43	-0.46608680330908925
2211	44	-0.494110068304213
2211	45	-0.4514693292876508
2211	46	-0.4749250341870379
2211	47	-0.6296832978105371
2211	48	-0.6170250942138686
2211	49	-0.5602124568956022
2211	50	-0.46960404680380563
2212	1	-0.2915906363052052
2212	2	-0.3856355062972696
2212	3	-0.32591712801636774
2212	4	-0.3272204295929093
2212	5	-0.33050228795585923
2212	6	-0.4307549012884593
2212	7	-0.2731126586683601
2212	8	-0.22454600342899766
2212	9	-0.5084919702914448
2212	10	-0.49367640310263994
2212	11	-0.30157881287068733
2212	12	-0.33560039150574994
2212	13	-0.33764977080540604
2212	14	-1.061847413889872
2212	15	-0.612961454433119
2212	16	-0.5996780801708326
2212	17	-0.4456859235191786
2212	18	-0.4541343450383266
2212	19	-0.4719990911975316
2212	20	-0.40497785940131337
2212	21	-0.500374031412747
2212	22	-0.5550607310061295
2212	23	-0.8374589627191682
2212	24	-0.5448055728484746
2212	25	-0.3318697815588578
2212	26	-0.5311712490052898
2212	27	-0.6465230665442192
2212	28	-0.7089525549714324
2212	29	-0.5806742622605086
2212	30	-0.6266499196599185
2212	31	-0.5011025848635953
2212	32	-0.4242088786553566
2212	33	-0.4356586299186702
2212	34	-0.4501536203834675
2212	35	-0.4001159469712334
2212	36	-0.6959201919591713
2212	37	-0.7228797900936321
2212	38	-0.6409214628085174
2212	39	-0.6086995990845239
2212	40	-0.5671488171311054
2212	41	-0.47472203425416026
2212	42	-0.5194156233960108
2212	43	-0.5969825793669337
2212	44	-0.4581558504425054
2212	45	-0.468475035507506
2212	46	-0.3700381765088423
2212	47	-0.3805260711755558
2212	48	-0.40129034815426223
2212	49	-0.35511831572388886
2212	50	-0.2936452896587909
2213	1	-0.21587075609507878
2213	2	-0.3379686608225647
2213	3	-0.29192207416679494
2213	4	-0.5189476728438223
2213	5	-0.32443063563150076
2213	6	-0.300859952840419
2213	7	-0.4020579967693507
2213	8	-0.2950965632059166
2213	9	-0.22263371369176355
2213	10	-0.49409873115187253
2213	11	-0.4011059263000618
2213	12	-0.4250117201927921
2213	13	-0.25279683073675585
2213	14	-0.2651014978068176
2213	15	-0.45273857319848076
2213	16	-0.3094352470354522
2213	17	-0.2938865721874393
2213	18	-0.3837628941005704
2213	19	-0.5095233150304566
2213	20	-0.3818780766703398
2213	21	-0.44181434740389847
2213	22	-0.2902321670031114
2213	23	-0.2551930369811046
2213	24	-0.3336520632725666
2213	25	-0.3700610435899492
2213	26	-0.4493696722511302
2213	27	-0.4886833333689182
2213	28	-0.6878569864183746
2213	29	-0.6004141358037813
2213	30	-0.7201712959565612
2213	31	-0.5555814122762825
2213	32	-0.6203622877890709
2213	33	-0.7255726718005562
2213	34	-0.6290403881284661
2213	35	-0.7757697628958375
2213	36	-0.9110018153687314
2213	37	-0.7647696790798939
2213	38	-0.7183384630269813
2213	39	-0.8421670881426024
2213	40	-0.6712279630711038
2213	41	-0.646742047675751
2213	42	-0.6060533349837769
2213	43	-0.7853494818425837
2213	44	-0.6491057260197337
2213	45	-0.6559337600422701
2213	46	-0.6555222466903174
2213	47	-0.7647702059790298
2213	48	-0.720329174399307
2213	49	-0.6641078609467321
2213	50	-0.6197962866103701
2214	1	-0.18722069361964344
2214	2	-0.491861039622691
2214	3	-0.40447835458572
2214	4	-0.39035699602041873
2214	5	-0.3001584497451404
2214	6	-0.1816510967156013
2214	7	-0.10238832559654298
2214	8	-0.25997255317276924
2214	9	-0.39511635837399695
2214	10	-0.24614432277139286
2214	11	-0.36343817419221125
2214	12	-0.37862706894291165
2214	13	-0.27937577225085164
2214	14	-0.3875287503830833
2214	15	-0.19635075337492225
2214	16	-0.3306886751510092
2214	17	-0.05696234574580927
2214	18	-0.28731999269434993
2214	19	-0.3962039737848073
2214	20	-0.28711806484264457
2214	21	-0.2735144208171718
2214	22	-0.31822082255149553
2214	23	-0.3056628077685559
2214	24	-0.466664955088099
2214	25	-0.4747793912228033
2214	26	-0.32890474161060723
2214	27	-0.529774836709592
2214	28	-0.4016201906110908
2214	29	-0.35883457015328446
2214	30	-0.419496711099967
2214	31	-0.4416818481830376
2214	32	-0.48279289123627933
2214	33	-0.3684768511044396
2214	34	-0.5530126950107714
2214	35	-0.48505320486598436
2214	36	-0.4756014395891768
2214	37	-0.4500635731719811
2214	38	-0.34760151551129326
2214	39	-0.36158549343083024
2214	40	-0.3268693528313129
2214	41	-0.32649128542599115
2214	42	-0.24242833683764786
2214	43	-0.3867455776530839
2214	44	-0.32941023661027913
2214	45	-0.3365776834255101
2214	46	-0.3174129953203071
2214	47	-0.21283834931535495
2214	48	-0.2585425051495811
2214	49	-0.23235702237930767
2214	50	-0.2388585324756214
2215	1	-0.07560173733395814
2215	2	-0.14774853057050663
2215	3	-0.1939260631675097
2215	4	-0.014381341568245887
2215	5	0.01602284613522187
2215	6	-0.05901468811353894
2215	7	-0.0519088176941338
2215	8	-0.012691116598702479
2215	9	-0.22018771727105957
2215	10	-0.20858669628393756
2215	11	-0.2778027283129134
2215	12	-0.12967878190542592
2215	13	-0.16456375445559956
2215	14	-0.2642797246997688
2215	15	-0.24859071624953089
2215	16	-0.28537226631939916
2215	17	-0.06625102282638551
2215	18	-0.11681623913028005
2215	19	-0.2642467430575195
2215	20	-0.19342057197000884
2215	21	-0.2284040659870641
2215	22	-0.15970550328567157
2215	23	-0.2678889680495615
2215	24	-0.292461923418756
2215	25	-0.310947421165082
2215	26	-0.3097381521075562
2215	27	-0.31147128543052816
2215	28	-0.2154166266291142
2215	29	-0.3388285645748539
2215	30	-0.2033083735548189
2215	31	-0.10664633973528481
2215	32	-0.27026714999637136
2215	33	-0.22558413986341463
2215	34	-0.26340146935628184
2215	35	-0.3458198327724773
2215	36	-0.3874443719989671
2215	37	-0.22428333267276201
2215	38	-0.2027151910912437
2215	39	-0.31154500385830985
2215	40	-0.3966944775843295
2215	41	-0.46275928684487155
2215	42	-0.29138807045798387
2215	43	-0.380250843934256
2215	44	-0.3681080234026268
2215	45	-0.314265920386525
2215	46	-0.3149410452646322
2215	47	-0.303961446766877
2215	48	-0.3465471455136111
2215	49	-0.33782607713197166
2215	50	-0.3479860733106861
2216	1	-0.36055726924424647
2216	2	-0.3185211480183838
2216	3	-0.4228731993426038
2216	4	-0.30491341004627515
2216	5	-0.27242027950274306
2216	6	-0.28670096536595935
2216	7	-0.3731852292083815
2216	8	-0.4083519602019088
2216	9	-0.3780534321324396
2216	10	-0.5657985029322532
2216	11	-0.7014717245379956
2216	12	-0.5780007172206325
2216	13	-0.5376003230277107
2216	14	-0.623805936811145
2216	15	-0.6291815315616702
2216	16	-0.6802338332679767
2216	17	-0.6615745050749524
2216	18	-0.5719997980095002
2216	19	-0.6683821671653689
2216	20	-0.8584337350574103
2216	21	-0.9677265624610882
2216	22	-0.6973073069866587
2216	23	-0.7683156652219552
2216	24	-0.9489351484834074
2216	25	-0.8925021974258982
2216	26	-0.7304942012164446
2216	27	-0.7811172558273185
2216	28	-0.7780041515856908
2216	29	-0.9238454576199828
2216	30	-0.8288337730682542
2216	31	-0.9666150680361767
2216	32	-0.8875255311614154
2216	33	-0.9426011561303618
2216	34	-0.9161025249010137
2216	35	-0.9959671994056691
2216	36	-0.8963299971748491
2216	37	-1.0478061278764343
2216	38	-1.1496577913713253
2216	39	-1.033415877384435
2216	40	-1.1407931516176646
2216	41	-1.1103936777211274
2216	42	-1.0122470264361567
2216	43	-1.1336309516034824
2216	44	-1.3675347921768868
2216	45	-1.410537294792686
2216	46	-1.5120867950277628
2216	47	-1.389497536605571
2216	48	-1.4663170037918718
2216	49	-1.486955365126338
2216	50	-1.4683165908589721
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	195843.0
3	9549.0
4	17298.0
5	13622.0
6	11080.0
7	9237.0
8	8479.0
9	7585.0
10	7291.0
11	7278.0
12	7399.0
13	7799.0
14	8224.0
15	8755.0
16	9361.0
17	10146.0
18	10975.0
19	12446.0
20	15356.0
21	19276.0
22	23565.0
23	26803.0
24	28678.0
25	31076.0
26	34713.0
27	38837.0
28	44911.0
29	50795.0
30	59592.0
31	70891.0
32	85175.0
33	105215.0
34	133355.0
35	170450.0
36	221619.0
37	299207.0
38	457930.0
39	733314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80025350925299	17.17734453707822	13.078147748195423	24.944254205473367
2	16.08052904699056	19.09756122678468	39.19939353733237	25.62251618889239
3	20.01788016700116	23.40630802458041	27.786085163680603	28.789726644737822
4	13.548246489773557	15.340544207607266	35.75226636890277	35.35894293371641
5	14.98375869452991	36.823879668835495	33.16425411248192	15.028107524152674
6	34.077282121851304	34.67666951804604	16.78998661091423	14.45606174918842
7	29.892778753656778	31.10575023327013	20.310307385282563	18.691163627790523
8	26.995540265458807	34.72612315243127	19.20163114043681	19.076705441673113
9	26.831071251026543	16.26915984433358	18.513697190359945	38.38607171427993
10	15.488422082262135	28.736497741724165	32.36292051526474	23.412159660748966
11	36.1326521227069	22.724729612573906	22.042921316453814	19.09969694826538
12	24.055241970029947	24.30499241882363	28.80837057507357	22.831395036072852
13	28.559849622612127	21.340685736561316	25.211488954626795	24.887975686199766
14	22.7384552346375	20.657005588053938	25.91941899380496	30.6851201835036
15	24.555526814824947	28.313264813808214	22.656435999613496	24.474772371753346
16	24.535431774002774	27.33794984967654	24.225188327700494	23.901430048620192
17	23.493264584545337	27.227096798187244	25.55141150187412	23.7282271153933
18	23.49685803581746	27.07561567488806	26.057449264351355	23.370077024943118
19	24.444555877160706	26.53472737875272	26.17670734477386	22.844009399312718
20	24.83406451441495	25.914064912939082	25.925954216219267	23.3259163564267
21	24.839201142307115	25.880470275004658	25.91464781687215	23.36568076581608
22	24.7122729655166	25.497248781498843	25.68763854907851	24.102839703906046
23	24.289196315310587	25.705092017613186	25.805145813547277	24.200565853528946
24	24.059829230154016	25.85281961554864	25.897292313496983	24.19005884080036
25	24.30699906530322	25.713960197993625	25.983839776895117	23.995200959808038
26	24.1963791640842	25.771070114917332	25.979328299264687	24.053222421733782
27	24.11828944099544	26.044920175418785	25.734144355152626	24.102646028433146
28	24.108470761056644	25.951773424723335	25.784079655869004	24.15567615835102
29	24.096610051827273	26.140603611865764	25.88291214607698	23.87987419022998
30	24.362930284867033	26.10824987096187	25.605549204215045	23.92327063995605
31	24.42916453456691	25.71287398634637	25.685379446237384	24.172582032849334
32	24.224616367479676	26.082395904377933	25.65637736144768	24.036610366694713
33	24.29302195074572	25.80226231647526	25.57473563970294	24.329980093076074
34	24.35301977232671	25.85360881154862	25.812502241270995	23.98086917485367
35	24.404427073821093	25.996739134044173	25.627351426379807	23.97148236575493
36	24.441924483066636	25.917363714548515	25.596428369821115	24.04428343256373
37	24.388300883938967	25.606967108730238	25.924858380152532	24.07987362717826
38	24.338758909766934	25.79068526554401	25.725573035767233	24.144982788921823
39	24.369952430570084	26.09226065364634	25.640407517869253	23.89737939791433
40	24.629318474637056	25.665486737418146	25.760755508919203	23.944439279025595
41	24.340457631547405	25.933870956496218	25.680542408626582	24.045129003329794
42	24.459475521434058	25.8855132600394	25.519508416716967	24.135502801809572
43	24.152431797129452	25.60573312483192	25.82299956506871	24.418835512969917
44	24.206055406632036	25.823360400745553	25.685046344836927	24.28553784778548
45	24.34757061096578	25.911103881445285	25.57322028496687	24.168105222622067
46	24.4329953375504	25.568681086123362	25.581063383753293	24.417260192572947
47	24.30888824558934	25.854610908530773	25.617379445517102	24.219121400362788
48	24.17209672423872	25.975111530710244	25.598335413071112	24.254456331979927
49	24.365865082819045	25.733374363591153	25.59491384143797	24.305846712151826
50	24.398791641077562	25.863063721015155	25.6583398343486	24.079804803558684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2304.0
1	1737.5
2	1171.0
3	1104.5
4	1038.0
5	1006.5
6	975.0
7	978.0
8	981.0
9	1060.0
10	1139.0
11	1325.0
12	1511.0
13	2154.0
14	2797.0
15	3624.5
16	4452.0
17	4818.5
18	5185.0
19	5372.5
20	5560.0
21	6331.0
22	7102.0
23	8826.0
24	10550.0
25	13558.5
26	16567.0
27	21330.5
28	26094.0
29	33148.0
30	40202.0
31	49505.0
32	58808.0
33	71162.5
34	83517.0
35	95955.5
36	108394.0
37	120743.0
38	133092.0
39	144877.0
40	156662.0
41	168884.0
42	181106.0
43	189382.5
44	197659.0
45	208364.5
46	219070.0
47	225489.5
48	231909.0
49	233058.5
50	234208.0
51	232929.0
52	231650.0
53	224928.5
54	218207.0
55	207533.5
56	196860.0
57	183134.0
58	169408.0
59	155368.5
60	141329.0
61	124092.5
62	106856.0
63	92002.5
64	77149.0
65	64636.0
66	52123.0
67	42755.0
68	33387.0
69	27566.0
70	21745.0
71	17180.5
72	12616.0
73	10597.5
74	8579.0
75	6778.0
76	4977.0
77	3855.0
78	2733.0
79	2154.5
80	1576.0
81	1213.5
82	851.0
83	654.0
84	457.0
85	343.5
86	230.0
87	186.5
88	143.0
89	110.0
90	77.0
91	53.5
92	30.0
93	27.5
94	25.0
95	22.5
96	20.0
97	15.0
98	10.0
99	22.0
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08575814146442648
2	0.0956482057664385
3	0.10241858535573532
4	0.10401161584733458
5	0.09621240406554657
6	0.05698402820991495
7	0.05293507571043352
8	0.057581414644264674
9	0.060203277328355106
10	0.04360920970752956
11	0.047392657125077786
12	0.057050404480398255
13	0.04984857913295996
14	0.05051234183779298
15	0.05064509437875959
16	0.05373159095623315
17	0.052470441817050406
18	0.05419622484961627
19	0.06109935697987969
20	0.06687409251192698
21	0.07885500933416303
22	0.08160962455922008
23	0.057913295996681186
24	0.07596764156813939
25	0.08373366521468575
26	0.08094586185438706
27	0.07543663140427298
28	0.0954490769549886
29	0.08456336859572702
30	0.07922007882182121
31	0.053698402820991496
32	0.05041277743206803
33	0.05343289773905829
34	0.04782410288321925
35	0.05492636382493258
36	0.053598838415266546
37	0.05701721634515661
38	0.046529765608794856
39	0.05067828251400124
40	0.05459448247251608
41	0.040688653806264255
42	0.03793403858120722
43	0.03833229620410703
44	0.03780128604024061
45	0.028043974279195187
46	0.02522298278365484
47	0.029105994606928025
48	0.024094586185438707
49	0.023696328562538893
50	0.025720804812279608
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3013125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.84751837247778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9345964808848	47.42126791222281
2	11.970080769482925	14.566994190823104
3	4.152983716096513	7.580962588973509
4	2.0072299277776926	4.885398396329617
5	1.0895710928906663	3.314884854639275
6	0.6558308699832891	2.3943408546327762
7	0.44840638164403807	1.9099090881795324
8	0.31153555688896273	1.5164932417185004
9	0.23400273140664585	1.281463694862828
>10	1.1367390312346985	11.957037624865173
>50	0.04701944832442593	1.927734541059508
>100	0.011675000391929785	1.065182367522686
>500	2.193286624618578E-4	0.08738034751280196
>1k	1.0966433101143897E-4	0.09095029665780736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.318813524165111E-5
2	0.0	0.0	0.0	0.0	3.318813524165111E-5
3	0.0	0.0	0.0	0.0	3.318813524165111E-5
4	0.0	0.0	0.0	0.0	3.318813524165111E-5
5	0.0	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
6	0.0	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
7	9.956440572495333E-5	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
8	9.956440572495333E-5	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
9	9.956440572495333E-5	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
10	9.956440572495333E-5	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
11	9.956440572495333E-5	3.318813524165111E-5	0.0	0.0	3.318813524165111E-5
12	9.956440572495333E-5	6.637627048330222E-5	0.0	0.0	1.3275254096660444E-4
13	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	1.6594067620825554E-4
14	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	1.6594067620825554E-4
15	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	2.3231694669155776E-4
16	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	2.3231694669155776E-4
17	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	2.3231694669155776E-4
18	9.956440572495333E-5	9.956440572495333E-5	0.0	0.0	2.655050819332089E-4
19	1.9912881144990667E-4	9.956440572495333E-5	0.0	0.0	2.655050819332089E-4
20	1.9912881144990667E-4	9.956440572495333E-5	0.0	0.0	2.9869321717486E-4
21	1.9912881144990667E-4	9.956440572495333E-5	0.0	6.637627048330222E-5	2.9869321717486E-4
22	2.3231694669155776E-4	9.956440572495333E-5	0.0	9.956440572495333E-5	3.650694876581622E-4
23	2.3231694669155776E-4	9.956440572495333E-5	0.0	1.3275254096660444E-4	3.650694876581622E-4
24	2.655050819332089E-4	9.956440572495333E-5	0.0	2.9869321717486E-4	3.9825762289981333E-4
25	2.655050819332089E-4	9.956440572495333E-5	0.0	2.9869321717486E-4	3.9825762289981333E-4
26	2.655050819332089E-4	9.956440572495333E-5	0.0	4.314457581414644E-4	4.314457581414644E-4
27	2.655050819332089E-4	9.956440572495333E-5	0.0	6.637627048330222E-4	4.314457581414644E-4
28	3.318813524165111E-4	9.956440572495333E-5	0.0	0.0015930304915992533	4.978220286247666E-4
29	4.314457581414644E-4	9.956440572495333E-5	0.0	0.002455922007882182	4.978220286247666E-4
30	4.6463389338311553E-4	9.956440572495333E-5	0.0	0.004447210122381248	4.978220286247666E-4
31	4.6463389338311553E-4	9.956440572495333E-5	0.0	0.011350342252644679	4.978220286247666E-4
32	4.6463389338311553E-4	9.956440572495333E-5	0.0	0.018087533706699854	5.310101638664178E-4
33	4.6463389338311553E-4	9.956440572495333E-5	0.0	0.027944409873470234	5.310101638664178E-4
34	4.978220286247666E-4	9.956440572495333E-5	0.0	0.04025720804812279	5.310101638664178E-4
35	5.310101638664178E-4	1.3275254096660444E-4	0.0	0.054826799419207636	5.641982991080688E-4
36	5.9738643434972E-4	1.3275254096660444E-4	0.0	0.07327940261356565	5.641982991080688E-4
37	6.305745695913711E-4	1.3275254096660444E-4	0.0	0.09933208877826177	5.641982991080688E-4
38	6.305745695913711E-4	1.3275254096660444E-4	0.0	0.1276083800041485	5.641982991080688E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2475	0.0	21.339447	1
GTATCAA	4445	0.0	20.595327	1
TATCAAC	5720	0.0	15.964787	2
TAGATCG	85	0.002978599	15.53154	5
TCAACGC	5865	0.0	15.157892	4
ATCAACG	5925	0.0	15.116066	3
TATATAC	415	0.0	14.847135	3
CAACGCA	6000	0.0	14.815364	5
GGTTACG	90	0.004352717	14.67087	1
AACGCAG	6095	0.0	14.653235	6
GTCCTAA	380	0.0	14.477833	1
GGACCGT	290	7.2759576E-12	13.653868	6
TACACCG	230	9.145879E-9	13.393141	5
GTATAGC	615	0.0	13.239566	1
ACCGTGC	400	0.0	13.198301	8
GTCTAGG	605	0.0	13.094661	1
GTACATG	9350	0.0	12.92135	1
GTCTTAG	685	0.0	12.850398	1
CGCTTCG	155	7.1620554E-5	12.777431	22
CTAGGAC	775	0.0	12.77743	3
>>END_MODULE