FastQCFastQC Report
Mon 6 Feb 2017
SRR2077142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013125
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49600.022.354611
GGTATCA26050.021.8730961
TACCGCG1451.2493001E-716.69170217
TATCAAC67850.016.24532
TATACTG6250.016.1931785
TCAACGC68750.015.9048944
ATCAACG68900.015.8700053
CAACGCA70350.015.4495975
AACGCAG73350.014.9072036
GTACATG89250.014.7651171
TACATGG90400.014.3589752
TTACGCT1150.001424125213.3920564
TTACCGC2003.2742355E-713.20161816
ACATGGG96450.013.0248813
TTATACT7100.013.0148164
GTCTTAG7450.012.9931561
TAGACTG6800.012.942125
GTCTATG6950.012.6617411
TGGACCG4200.012.5723455
ACGCAGA87100.012.5037817
Produced by FastQC (version 0.11.5)