FastQCFastQC Report
Mon 6 Feb 2017
SRR2077141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340822
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45400.022.2002411
GGTATCA23500.020.8826241
GGACCGT3550.016.730466
TATCAAC60300.016.2401772
TCAACGC59850.015.8838084
ATCAACG61050.015.7527673
CAACGCA61200.015.4601265
GACCGTA1004.95129E-415.3982187
AACGCAG62850.015.1199676
GTACATG113650.013.6123761
TACATGG112850.013.4748692
TTAATAC6700.013.4669773
CGTATAC1000.00874630413.2042073
GTATCGC1353.2130466E-413.0355296
ATTATAC5400.012.6336553
ACCGTGC5050.012.6322028
ACATGGG117950.012.6314093
GTTCGAC1404.3702786E-412.56903311
GTGTAGC12150.012.4974341
GTGTAGG7800.012.4138391
Produced by FastQC (version 0.11.5)