##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077141_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3340822
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.95901278188422	31.0	30.0	34.0	23.0	34.0
2	29.115914885617972	31.0	30.0	34.0	22.0	34.0
3	29.178659024635255	31.0	30.0	34.0	22.0	34.0
4	32.58439180537005	35.0	33.0	37.0	24.0	37.0
5	32.54286310375111	35.0	33.0	37.0	24.0	37.0
6	32.60487628493826	36.0	33.0	37.0	25.0	37.0
7	32.4564238980706	36.0	33.0	37.0	23.0	37.0
8	32.34288896564977	36.0	33.0	37.0	22.0	37.0
9	33.70829634143932	38.0	34.0	39.0	22.0	39.0
10	33.65753129020343	38.0	34.0	39.0	21.0	39.0
11	33.67535205407531	38.0	34.0	39.0	22.0	39.0
12	33.58552805267686	38.0	34.0	39.0	19.0	39.0
13	33.52627197737563	38.0	34.0	39.0	19.0	39.0
14	34.44512188916381	39.0	34.0	41.0	18.0	41.0
15	34.486551214042535	39.0	34.0	41.0	18.0	41.0
16	34.44581213844976	39.0	34.0	41.0	18.0	41.0
17	34.28998432122394	39.0	33.0	41.0	17.0	41.0
18	34.26619646302616	39.0	33.0	41.0	17.0	41.0
19	34.40193521235193	39.0	34.0	41.0	18.0	41.0
20	34.37889956423898	39.0	34.0	41.0	18.0	41.0
21	34.330253751920935	39.0	34.0	41.0	18.0	41.0
22	34.24882588776056	39.0	34.0	41.0	18.0	41.0
23	34.206791621942145	39.0	34.0	41.0	18.0	41.0
24	34.21545416068261	39.0	34.0	41.0	18.0	41.0
25	34.12157067931186	39.0	34.0	41.0	17.0	41.0
26	34.069614603831035	39.0	33.0	41.0	17.0	41.0
27	34.0057629529499	39.0	33.0	41.0	16.0	41.0
28	33.895509847576434	39.0	33.0	41.0	16.0	41.0
29	33.88645399246054	39.0	33.0	41.0	16.0	41.0
30	33.72896191416364	39.0	33.0	41.0	13.0	41.0
31	33.7223240268413	38.0	33.0	41.0	14.0	41.0
32	33.59244311729269	38.0	33.0	40.0	10.0	41.0
33	33.54861647822003	38.0	33.0	40.0	9.0	41.0
34	33.47428716645185	38.0	33.0	40.0	9.0	41.0
35	33.40423374846071	38.0	33.0	40.0	9.0	41.0
36	33.31542177344378	38.0	33.0	40.0	9.0	41.0
37	33.22594199870571	38.0	33.0	40.0	8.0	41.0
38	33.14209526876918	38.0	32.0	40.0	8.0	41.0
39	33.02900813033439	38.0	32.0	40.0	6.0	41.0
40	32.97134597413451	38.0	32.0	40.0	2.0	41.0
41	32.860093414135804	38.0	32.0	40.0	2.0	41.0
42	32.71744199481445	38.0	32.0	40.0	2.0	41.0
43	32.638888872259585	38.0	32.0	40.0	2.0	41.0
44	32.497605679081374	38.0	32.0	40.0	2.0	41.0
45	32.35197535217381	38.0	31.0	40.0	2.0	41.0
46	32.22317471568375	38.0	31.0	40.0	2.0	41.0
47	32.068019786747094	38.0	31.0	40.0	2.0	41.0
48	31.903318105544084	38.0	31.0	40.0	2.0	41.0
49	31.72957703223937	38.0	31.0	40.0	2.0	41.0
50	31.518482277714885	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.6613505774073438
1101	2	2.0374414474994964
1101	3	2.0354002528781727
1101	4	2.2912597978085287
1101	5	2.3580322240145435
1101	6	2.350180667113392
1101	7	2.4361595693989244
1101	8	2.369256817773845
1101	9	2.676377817203381
1101	10	2.7135749341715893
1101	11	2.7214075525445693
1101	12	2.851149877673535
1101	13	2.78798776102947
1101	14	3.2214392413146484
1101	15	3.1956384778197418
1101	16	3.203901760057505
1101	17	3.3365506010707335
1101	18	3.242292746958398
1101	19	3.2566730835385513
1101	20	3.305636705296166
1101	21	3.281266817954254
1101	22	3.3438534188506637
1101	23	3.3048968424894056
1101	24	3.349039207343594
1101	25	3.2377128198009544
1101	26	3.3079549465726856
1101	27	3.3337764210121605
1101	28	3.508562182464871
1101	29	3.4493747150599603
1101	30	3.5933818996694242
1101	31	3.5781687243973863
1101	32	3.635697377339234
1101	33	3.5686760375160347
1101	34	3.601470564099735
1101	35	3.6648168614674432
1101	36	3.659255564035078
1101	37	3.5792818558604367
1101	38	3.754967757087954
1101	39	3.7338186219773704
1101	40	3.74443364633764
1101	41	3.830552956538604
1101	42	3.776607794129852
1101	43	3.750006513541919
1101	44	3.6969221991537182
1101	45	3.776567578013818
1101	46	3.7915687592182223
1101	47	3.785626910831162
1101	48	3.6866087395287153
1101	49	3.6665476693211474
1101	50	3.765142618426335
1102	1	0.9790114635713998
1102	2	1.1039767092705297
1102	3	1.0635121423817182
1102	4	1.2892761994607866
1102	5	1.3487230126436245
1102	6	1.2244276154008773
1102	7	1.3928904473529045
1102	8	1.3461289391747684
1102	9	1.4771434651515918
1102	10	1.6283153445587217
1102	11	1.4054941701131085
1102	12	1.4903485977387305
1102	13	1.5299587523561797
1102	14	1.765349325797274
1102	15	1.7253654200980506
1102	16	1.668243297185839
1102	17	1.91332424882021
1102	18	1.9290130058734007
1102	19	1.922340841983356
1102	20	1.8297363432078768
1102	21	1.7574922015419006
1102	22	1.8209105262623027
1102	23	1.8371841553715456
1102	24	1.672390579103407
1102	25	1.8793795044431931
1102	26	1.756778324907799
1102	27	1.7638333042974352
1102	28	1.8973020210201312
1102	29	1.7837899689570023
1102	30	1.9087400109459054
1102	31	1.8994491713308292
1102	32	1.9270448123796413
1102	33	1.9381700943403146
1102	34	2.0725394408940474
1102	35	1.9465076579593799
1102	36	1.9916934124581616
1102	37	1.914673078832628
1102	38	1.928102973198719
1102	39	2.019509945350663
1102	40	1.9587355386363328
1102	41	1.9380173386425312
1102	42	1.9810189015847683
1102	43	1.892883835425998
1102	44	1.646614145432693
1102	45	1.77435025086789
1102	46	1.799801454293572
1102	47	1.6996069198170858
1102	48	1.7112069037800097
1102	49	1.7229107531969419
1102	50	1.7947178352969466
1103	1	0.29065756947963806
1103	2	0.46507968848540315
1103	3	0.32338684365663894
1103	4	0.3544822507432528
1103	5	0.4554767983969299
1103	6	0.46170054655136283
1103	7	0.4162924166325368
1103	8	0.44830456160416077
1103	9	0.6509412307018749
1103	10	0.565055807026944
1103	11	0.5500627643630907
1103	12	0.48689114510518294
1103	13	0.5469992308962475
1103	14	0.5917551575648119
1103	15	0.558766362136673
1103	16	0.49323813784471326
1103	17	0.665074028950535
1103	18	0.45794217961070416
1103	19	0.5577995359435803
1103	20	0.5984476307974234
1103	21	0.41095074928223596
1103	22	0.4553145244200536
1103	23	0.6322733263695426
1103	24	0.5674287582277131
1103	25	0.5822381650257924
1103	26	0.5210305227562699
1103	27	0.5633482491117618
1103	28	0.5449509501445107
1103	29	0.5267193580138922
1103	30	0.6564233973739206
1103	31	0.5032244208686478
1103	32	0.7248023421642316
1103	33	0.6912805832114088
1103	34	0.6717852670719111
1103	35	0.6243559304515642
1103	36	0.6281346177172651
1103	37	0.6322138823572061
1103	38	0.5290827698666973
1103	39	0.6958842966541354
1103	40	0.5910702476911354
1103	41	0.6012778633128235
1103	42	0.6459375104721587
1103	43	0.6188822566213403
1103	44	0.5605461556508118
1103	45	0.49949664813622974
1103	46	0.4813829612407119
1103	47	0.5293363235576649
1103	48	0.4763746725971103
1103	49	0.4839087872489074
1103	50	0.5031282245595925
1104	1	0.4745406497795841
1104	2	0.5239960352454247
1104	3	0.5127019486622295
1104	4	0.453274794012529
1104	5	0.5021707269160061
1104	6	0.39096151840176674
1104	7	0.4804093595423993
1104	8	0.6806558932129008
1104	9	0.6908941981110317
1104	10	0.49104590707720774
1104	11	0.6328663915054307
1104	12	0.5095469666919925
1104	13	0.5409212742876548
1104	14	0.7397984648328588
1104	15	0.6683662321319943
1104	16	0.8029612950613654
1104	17	0.8251094045646212
1104	18	0.6606426852832143
1104	19	0.6809940296251753
1104	20	0.6286098535857505
1104	21	0.5430779686316853
1104	22	0.5779368442871302
1104	23	0.6311852922785022
1104	24	0.6373929730944354
1104	25	0.6716177181603911
1104	26	0.6311625445272142
1104	27	0.7159531017393448
1104	28	0.5616857044908343
1104	29	0.6289310331642284
1104	30	0.6109167025534603
1104	31	0.6554573586570243
1104	32	0.6448204175452972
1104	33	0.5837476506712704
1104	34	0.5251553775345954
1104	35	0.531514842903988
1104	36	0.6240925777641877
1104	37	0.6892917817967756
1104	38	0.758314948910126
1104	39	0.7190219821015162
1104	40	0.7038544734434069
1104	41	0.6764055907115534
1104	42	0.5952301451114508
1104	43	0.69943233797283
1104	44	0.649940177719543
1104	45	0.6727480746559706
1104	46	0.6669266957886606
1104	47	0.6648738223297599
1104	48	0.7309280071154802
1104	49	0.7919571560402403
1104	50	0.8890229553643287
1105	1	0.11090495855230387
1105	2	0.12202424926556787
1105	3	0.01624617007730933
1105	4	0.1480347542975906
1105	5	0.02927351184403193
1105	6	0.04080470371300038
1105	7	0.08105472549827653
1105	8	0.0984203089290574
1105	9	0.0481851859132405
1105	10	-0.01930835717747925
1105	11	0.0849770592177066
1105	12	0.09399552686193147
1105	13	0.08478826189129762
1105	14	0.081695131532598
1105	15	0.04766763911341343
1105	16	0.02012939641787881
1105	17	0.026087847714464374
1105	18	-0.02212310358603986
1105	19	0.0940580051891331
1105	20	0.20841991929049897
1105	21	0.18356034986656766
1105	22	0.08311569218909654
1105	23	0.008290792219845855
1105	24	0.13610205271416476
1105	25	0.03863819711291683
1105	26	0.08780225272960251
1105	27	0.1170235815293168
1105	28	0.16332547296758548
1105	29	0.17412220761782748
1105	30	0.18110584208057645
1105	31	0.06280704017547123
1105	32	0.212275092380402
1105	33	0.17169471975304873
1105	34	0.1986497248484156
1105	35	0.14376673967061038
1105	36	0.19177803762147505
1105	37	0.23815944344203643
1105	38	0.15640890643550875
1105	39	0.3653262977920946
1105	40	0.29979811908363985
1105	41	0.15914921852906616
1105	42	0.18599460502880305
1105	43	0.27346989775513464
1105	44	0.14786209905712866
1105	45	0.24057477921405024
1105	46	0.16683013234833055
1105	47	0.06222553397407893
1105	48	0.17063129509795516
1105	49	0.22151525535169014
1105	50	0.2889279885723042
1106	1	0.11434162685187488
1106	2	0.030495079000967706
1106	3	0.086685705484296
1106	4	0.02446356951949724
1106	5	0.052533251502659084
1106	6	0.008364027997451728
1106	7	0.03171135236723899
1106	8	0.15566629130307064
1106	9	0.12421885845591163
1106	10	0.15124676589297792
1106	11	-0.04097733137169968
1106	12	0.1948403004558088
1106	13	0.2062287507202285
1106	14	0.3182250707759451
1106	15	0.27275641025745045
1106	16	0.15986205011780896
1106	17	0.27628934248627957
1106	18	0.025777081785662403
1106	19	0.191873882146389
1106	20	0.022880426768999484
1106	21	0.21229240478930933
1106	22	0.13945966912378083
1106	23	0.13625740786095264
1106	24	0.1391684034677496
1106	25	0.1680054501232604
1106	26	0.13777079196947284
1106	27	0.15270485865257655
1106	28	0.14509484734001887
1106	29	0.16939558881284
1106	30	0.14924149900728878
1106	31	0.06791050394477338
1106	32	0.29295569337481453
1106	33	0.2209391344296776
1106	34	0.2170265716278479
1106	35	0.1639222555869324
1106	36	0.2387441040518894
1106	37	0.21439691804182814
1106	38	0.367788004720488
1106	39	0.28787886323156187
1106	40	0.3972472389049102
1106	41	0.29240237980663153
1106	42	0.20923183033008286
1106	43	0.23380427749994226
1106	44	0.27505816518106485
1106	45	0.2727137625410734
1106	46	0.23028889054852897
1106	47	0.23188057143413232
1106	48	0.2519495585421865
1106	49	0.23315112900963442
1106	50	0.3131764052195827
1107	1	-0.1473288486002069
1107	2	-0.20791911596733925
1107	3	-0.22988198533474602
1107	4	-0.33054200184950133
1107	5	-0.34549139589069
1107	6	-0.332869180073466
1107	7	-0.2659982159159924
1107	8	-0.300996011793238
1107	9	-0.26997915632710345
1107	10	-0.4580224176987784
1107	11	-0.4504402331376838
1107	12	-0.4714203079039265
1107	13	-0.3579182930231113
1107	14	-0.5122281539357516
1107	15	-0.41529486404620997
1107	16	-0.45437425727787684
1107	17	-0.3507006545059852
1107	18	-0.30422256127040725
1107	19	-0.4199701399974174
1107	20	-0.23933840033538445
1107	21	-0.30173957295952647
1107	22	-0.6430983211214141
1107	23	-0.7024027602125997
1107	24	-0.5985716101957337
1107	25	-0.45312236541033
1107	26	-0.23613226261278442
1107	27	-0.2769502089966025
1107	28	-0.46013561253934654
1107	29	-0.3442135902354906
1107	30	-0.42780540148809365
1107	31	-0.4413422989643436
1107	32	-0.3229990638438238
1107	33	-0.28130186470342267
1107	34	-0.34437214221186707
1107	35	-0.2612322748477922
1107	36	-0.3346086343029526
1107	37	-0.2882379645665054
1107	38	-0.31750961319067983
1107	39	-0.254820485110578
1107	40	-0.24755322252121204
1107	41	-0.11079961476283984
1107	42	-0.40790699844557565
1107	43	-0.3903220745635352
1107	44	-0.14636000625329615
1107	45	0.01128678372278813
1107	46	-0.20676674538858464
1107	47	-0.21313166288821606
1107	48	-0.3195182253313078
1107	49	-0.30891374458102305
1107	50	-0.2585253982880431
1108	1	-0.03563379900013075
1108	2	-0.12003532872439138
1108	3	-0.049648482378643166
1108	4	-0.22672782285001603
1108	5	-0.19102248215124717
1108	6	-0.18917502948550435
1108	7	-0.23253345067139009
1108	8	-0.09022927798660874
1108	9	-0.09260760010968028
1108	10	-0.16354135700173345
1108	11	-0.08165396812126602
1108	12	-0.19926964445357243
1108	13	-0.18310377432057123
1108	14	-0.2437772684774444
1108	15	-0.2092326743683799
1108	16	-0.2667714971047985
1108	17	-0.2964815082929846
1108	18	-0.273253378170466
1108	19	-0.31040221482816577
1108	20	-0.4657923808659419
1108	21	-0.2937844480757619
1108	22	-0.3888211508821442
1108	23	-0.44886435832673754
1108	24	-0.32440777724728775
1108	25	-0.3069246719597345
1108	26	-0.2599414661635393
1108	27	-0.39189233938081003
1108	28	-0.526668925742122
1108	29	-0.6016889102940937
1108	30	-0.6379377484244131
1108	31	-0.536322482953004
1108	32	-0.29034227716017824
1108	33	-0.4324311543122832
1108	34	-0.358478362895319
1108	35	-0.2667506308222798
1108	36	-0.43717867661142407
1108	37	-0.45678008946056536
1108	38	-0.3448938653807403
1108	39	-0.3286855852543482
1108	40	-0.3942858968077516
1108	41	-0.25707139092941134
1108	42	-0.21567875230840627
1108	43	-0.2789756151957974
1108	44	-0.26638124627237403
1108	45	-0.26788372318965514
1108	46	-0.24038200937903298
1108	47	-0.2569480722612738
1108	48	-0.26366754369483303
1108	49	-0.37208725735077053
1108	50	-0.35709401315742895
1109	1	-0.12796154662403225
1109	2	-0.20662495345817078
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2210	36	-0.362266422789844
2210	37	-0.3074842982127919
2210	38	-0.29361992522964186
2210	39	-0.24016200752031125
2210	40	-0.5048743678202783
2210	41	-0.3757725824859719
2210	42	-0.4246223802504687
2210	43	-0.5087565149329691
2210	44	-0.4836379554076444
2210	45	-0.2791772279688445
2210	46	-0.36186703536698417
2210	47	-0.285369583923444
2210	48	-0.2406135759646837
2210	49	-0.04241573095620055
2210	50	-0.010046975548558379
2211	1	-0.3115083180886167
2211	2	-0.3502801179688859
2211	3	-0.3417746397371175
2211	4	-0.36892881215369755
2211	5	-0.48165369172297545
2211	6	-0.44592723850761473
2211	7	-0.2948553548695898
2211	8	-0.2791059532497897
2211	9	-0.2984791597427048
2211	10	-0.3399900104494975
2211	11	-0.387781732041276
2211	12	-0.5099608886932074
2211	13	-0.4415022861372506
2211	14	-0.710009036117917
2211	15	-0.457108208127309
2211	16	-0.5048706811406518
2211	17	-0.3736750989580173
2211	18	-0.6046532006031384
2211	19	-0.4761596240362991
2211	20	-0.6225234509204753
2211	21	-0.5508300659677801
2211	22	-0.5105714573971767
2211	23	-0.639537000274899
2211	24	-0.4296485500879399
2211	25	-0.5476366323875865
2211	26	-0.6088477359813211
2211	27	-0.5835652772745235
2211	28	-0.3784966008784707
2211	29	-0.4131479218359715
2211	30	-0.8881360319402489
2211	31	-0.5748782686884297
2211	32	-0.49651570125014644
2211	33	-0.5373979661331916
2211	34	-0.4927953628530375
2211	35	-0.44599977469409424
2211	36	-0.4262565948459027
2211	37	-0.28096983283349886
2211	38	-0.3773041304633935
2211	39	-0.35494863856682457
2211	40	-0.4214494701388318
2211	41	-0.3799632016933714
2211	42	-0.47314714955188464
2211	43	-0.4972625132636921
2211	44	-0.5594671127586537
2211	45	-0.3760526658184986
2211	46	-0.5421390803636825
2211	47	-0.5658957744554982
2211	48	-0.6674835501210765
2211	49	-0.5440941909321353
2211	50	-0.5016422734203978
2212	1	-0.0526459193547808
2212	2	-0.16123564133387092
2212	3	-0.1614110204442376
2212	4	-0.12414583023406323
2212	5	-0.08732499919654657
2212	6	-0.12761127466835376
2212	7	-0.125148624358296
2212	8	0.08178270762257966
2212	9	-0.038302044554122006
2212	10	-0.05197938756200671
2212	11	-0.0969509471279224
2212	12	-0.1130237541902801
2212	13	0.015143456660105414
2212	14	-0.747975128719446
2212	15	-0.4078168700726934
2212	16	-0.34267870463976635
2212	17	-0.2007369048488883
2212	18	-0.16815151059830669
2212	19	-0.1271256093359412
2212	20	-0.06908670538692263
2212	21	-0.14106686783483724
2212	22	-0.13244937466741646
2212	23	-0.23919182349503387
2212	24	-0.17255415124679985
2212	25	0.08673491383270004
2212	26	-0.2049805086622598
2212	27	-0.29759426219860785
2212	28	-0.26194421072878527
2212	29	-0.16777974221260195
2212	30	-0.10696033850175724
2212	31	0.06651330274780776
2212	32	-0.061726214153203784
2212	33	-0.0922282950134985
2212	34	-0.07875102034539339
2212	35	-0.09185785452670103
2212	36	-0.2238914599137658
2212	37	-0.19653303364898989
2212	38	-0.3323638171418608
2212	39	-0.13199904363147397
2212	40	-0.17716564321678163
2212	41	-0.2015650848416044
2212	42	-0.15070455606511501
2212	43	-0.21369921855904295
2212	44	-0.15317177770849355
2212	45	-0.08867666656961148
2212	46	-0.038028747191305
2212	47	-0.0063410102514112054
2212	48	-0.07949700309602292
2212	49	-0.013239128965288671
2212	50	-0.011633756332571465
2213	1	-0.4393839165586755
2213	2	-0.5911763519539583
2213	3	-0.4042076378380628
2213	4	-0.7940945114609406
2213	5	-0.49761304925366545
2213	6	-0.5129714651104464
2213	7	-0.6227100236047107
2213	8	-0.6112045436689186
2213	9	-0.7125711569609905
2213	10	-0.8069279835417476
2213	11	-0.6849409050158428
2213	12	-0.7520964572063278
2213	13	-0.6059181258676389
2213	14	-0.6614279785310515
2213	15	-0.8038031154480336
2213	16	-0.7234504682324214
2213	17	-0.8334379175848099
2213	18	-0.7182032982306552
2213	19	-0.7928279178282622
2213	20	-0.7740985973617569
2213	21	-0.9003486820642905
2213	22	-0.6755993414210337
2213	23	-0.7726358715403165
2213	24	-0.8117286180259953
2213	25	-0.7435460394007194
2213	26	-0.8764354001426611
2213	27	-0.8763887185776511
2213	28	-1.0085204728125632
2213	29	-0.9534268958108854
2213	30	-1.116812242922677
2213	31	-0.9699021406225228
2213	32	-0.9686997491812477
2213	33	-1.0128419038655636
2213	34	-1.124183479043161
2213	35	-1.0862491260729144
2213	36	-1.3355380295692
2213	37	-1.1908438532149148
2213	38	-1.0793258224953846
2213	39	-1.0317207117473401
2213	40	-1.0137218509128942
2213	41	-0.9757805981145893
2213	42	-0.9605459111535133
2213	43	-1.0395281403533225
2213	44	-0.9938741927597263
2213	45	-0.9471680267728395
2213	46	-0.9656027843249539
2213	47	-1.009660853226194
2213	48	-0.8975758950538086
2213	49	-1.0321352212367856
2213	50	-0.9095039633126056
2214	1	-0.2178237779660428
2214	2	-0.5233796247280793
2214	3	-0.42954740948281867
2214	4	-0.4741598014533537
2214	5	-0.3753479219756244
2214	6	-0.3095022043532083
2214	7	-0.3535100747704476
2214	8	-0.4246259655107707
2214	9	-0.6182479809251973
2214	10	-0.45852689968702975
2214	11	-0.5867394827445551
2214	12	-0.6764886415223756
2214	13	-0.48093462249682517
2214	14	-0.6519379652761401
2214	15	-0.4593413546344962
2214	16	-0.4840156754798315
2214	17	-0.3256470898677506
2214	18	-0.47942778148028964
2214	19	-0.5418637487687121
2214	20	-0.4129321338043823
2214	21	-0.5650515843188799
2214	22	-0.5013382136590891
2214	23	-0.5266265828906782
2214	24	-0.7460340326910213
2214	25	-0.6930643247358432
2214	26	-0.670701747909412
2214	27	-0.661291920023487
2214	28	-0.5375863878439304
2214	29	-0.5868452173338525
2214	30	-0.5051369696416117
2214	31	-0.43989225180980895
2214	32	-0.615915209822127
2214	33	-0.609631195039448
2214	34	-0.6777233872695305
2214	35	-0.753051420780217
2214	36	-0.747165390616523
2214	37	-0.67587434055676
2214	38	-0.6571360363702539
2214	39	-0.598676438198666
2214	40	-0.6972274409135295
2214	41	-0.6541540016729357
2214	42	-0.5379405998149878
2214	43	-0.669119178441683
2214	44	-0.5588055471434572
2214	45	-0.5501466633761893
2214	46	-0.6086794567296536
2214	47	-0.48220136292490423
2214	48	-0.5423541423890796
2214	49	-0.41100348439310963
2214	50	-0.39198460857694783
2215	1	-0.009828503383090492
2215	2	-0.1638347898651773
2215	3	-0.11572021852754233
2215	4	-0.06450084636544773
2215	5	-0.09270070740034697
2215	6	-0.013713485752120391
2215	7	0.03430702272542874
2215	8	0.01776606830433991
2215	9	0.03759845615263657
2215	10	-0.050548310316841594
2215	11	-0.030328577580448268
2215	12	0.06814697728063379
2215	13	-0.006134878040647607
2215	14	0.08722256060030986
2215	15	0.07084058274079297
2215	16	0.006714069874014683
2215	17	0.09020911501172435
2215	18	0.08984908352272214
2215	19	-0.047108534905255794
2215	20	-0.05948351693738374
2215	21	0.051614696652443115
2215	22	0.032586406193090056
2215	23	-0.14789951463801998
2215	24	-0.13044759139617668
2215	25	-0.1542660713918096
2215	26	-0.1323450540444071
2215	27	-0.1335467511151549
2215	28	-0.07115120060986868
2215	29	-0.14858338521187875
2215	30	-0.054498082738419384
2215	31	-0.14888746437037526
2215	32	-0.09224620234412839
2215	33	-0.10056792843977291
2215	34	-0.08354780108958693
2215	35	-0.09762410955237044
2215	36	-0.15424763259027685
2215	37	-0.12036811895827526
2215	38	-0.09968481591484846
2215	39	-0.059893955985522496
2215	40	-0.10232725173398904
2215	41	-0.17412294091342773
2215	42	-0.10890671386659534
2215	43	-0.1205847944635039
2215	44	-0.12198851154122536
2215	45	-0.06047454937164076
2215	46	-0.15228211151951854
2215	47	-0.08104652347453367
2215	48	-0.1616311248709259
2215	49	-0.158077545334109
2215	50	-0.10975433120491829
2216	1	-0.2526425966540913
2216	2	-0.21325096101836394
2216	3	-0.24276117613650072
2216	4	-0.1685843073793052
2216	5	-0.2087745917927606
2216	6	-0.2540693977900119
2216	7	-0.2617141317414706
2216	8	-0.3464947636530127
2216	9	-0.20982466875531713
2216	10	-0.24433853217679058
2216	11	-0.5767643876556505
2216	12	-0.40109261411064523
2216	13	-0.4345359366283077
2216	14	-0.5018202393314724
2216	15	-0.5500693492142688
2216	16	-0.46274612155868056
2216	17	-0.3705469819897971
2216	18	-0.43310854604406757
2216	19	-0.49453411978956296
2216	20	-0.6228131481171246
2216	21	-0.6317328591117288
2216	22	-0.4475679340528913
2216	23	-0.5585058688899025
2216	24	-0.7476383715377892
2216	25	-0.7845810248234599
2216	26	-0.599096412599593
2216	27	-0.6614536536425817
2216	28	-0.7280705655769424
2216	29	-0.919753426423128
2216	30	-0.6446514955068281
2216	31	-0.8729865562069392
2216	32	-0.7013064282164976
2216	33	-0.7816896377643161
2216	34	-0.7940184909700108
2216	35	-0.860132640517655
2216	36	-0.8207288591477848
2216	37	-0.995376055706302
2216	38	-0.9779012266830307
2216	39	-0.9059421535708267
2216	40	-1.1428465527416805
2216	41	-1.1425510725381223
2216	42	-1.054698442295841
2216	43	-1.1736455535523689
2216	44	-1.1532003258103494
2216	45	-1.2482679472605156
2216	46	-1.2268372131614242
2216	47	-1.1971575402126788
2216	48	-1.3249446997185323
2216	49	-1.4814726185333669
2216	50	-1.558410668851966
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	169487.0
3	8375.0
4	15861.0
5	12478.0
6	10389.0
7	9060.0
8	8192.0
9	7495.0
10	7337.0
11	7367.0
12	7491.0
13	7866.0
14	8278.0
15	8742.0
16	9462.0
17	10024.0
18	11069.0
19	12630.0
20	15636.0
21	20223.0
22	24611.0
23	27388.0
24	30417.0
25	33082.0
26	36579.0
27	41487.0
28	47238.0
29	54145.0
30	63461.0
31	77394.0
32	93511.0
33	114797.0
34	146935.0
35	190426.0
36	250114.0
37	341960.0
38	527721.0
39	872094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84983805991858	17.324428101809396	13.039749092485447	24.78598474578658
2	16.080438897076647	19.269549522881295	39.13925382583317	25.51075775420889
3	19.897447053063495	23.577379743141215	27.846663778188873	28.678509425606414
4	13.334611788958938	15.518313876836782	36.14575592684038	35.001318407363904
5	14.864964871601352	36.95206724086945	33.325822975881266	14.857144911647936
6	33.759203280370635	34.90718924337452	17.07944344394591	14.25416403230893
7	29.817033744221007	31.18434659636284	20.299661788105592	18.69895787131057
8	26.856265724212292	34.601743141248356	19.36006948604289	19.181921648496466
9	26.771231400089796	16.07712727323916	18.65096159001172	38.50067973665933
10	15.315543284261466	28.88570128050022	32.572020650910964	23.22673478432735
11	36.10503177426793	22.613995052677605	22.19655723860349	19.084415934450973
12	23.936584419131776	24.442235442953052	28.967367344102662	22.653812793812506
13	28.69958111479707	21.3042073300568	25.068184520324483	24.92802703482165
14	22.632651423218327	20.82372481770275	26.09842554114162	30.445198217937307
15	24.60449757475493	28.34302273345305	22.669243052801942	24.383236638990084
16	24.446198811669113	27.361829355109318	24.421250990949602	23.77072084227197
17	23.468502114114884	27.286307879098626	25.539280488148197	23.705909518638293
18	23.428876912456406	26.991058606484348	26.288655316842195	23.291409164217054
19	24.420091455497438	26.566183630442396	26.20684924818932	22.80687566587084
20	24.800503216240706	26.06004148538651	25.94475105023925	23.194704248133533
21	24.937300412804735	25.939804801476274	25.804639709538847	23.318255076180147
22	24.66236316129098	25.743052688782146	25.705306145436836	23.88927800449004
23	24.214302593498537	25.77597848839525	25.771905228418536	24.237813689687673
24	23.881780499911482	25.939307375672925	25.952488009367837	24.22642411504776
25	24.307472460972022	25.822246321888322	25.880065548428842	23.99021566871081
26	24.070742695852005	26.02345847254805	25.92573730501077	23.980061526589175
27	24.129550629417178	26.220378539994194	25.662061555061683	23.988009275526945
28	24.035661296778787	26.050148249173667	25.812074697449443	24.10211575659811
29	24.066588436119027	26.140287124284413	25.924858530430072	23.868265909166485
30	24.261837280216792	26.21769924918794	25.764390065183417	23.75607340541185
31	24.382996132326312	25.858815834161437	25.65321276877397	24.104975264738282
32	24.06912703237728	26.18238012380693	25.633754308834494	24.1147385349813
33	24.192702078300606	25.996337801566828	25.52712237097699	24.28383774915558
34	24.245798344493824	26.01309910276836	25.804783238898406	23.93631931383941
35	24.38919858529656	25.973626904163694	25.723157667434478	23.91401684310527
36	24.266386578782804	26.124873912533037	25.63882319961473	23.969916309069433
37	24.422682440974384	25.771082053175842	25.768446412788602	24.037789093061168
38	24.25849154865591	25.98481719874951	25.69950703362779	24.057184218966793
39	24.391961219689406	26.07194818590159	25.6125311244577	23.923559469951307
40	24.521019094545494	25.825974763429617	25.841877899851575	23.81112824217332
41	24.32688671010199	25.97911403075609	25.673906852518037	24.02009240662388
42	24.33888797890215	25.98360167295156	25.674632061750803	24.002878286395486
43	24.229852001264867	25.688368995486734	25.825635546526893	24.25614345672151
44	24.150634465820712	26.008292657072847	25.722957460831164	24.118115416275277
45	24.35487430135398	25.85826382179096	25.623463068584158	24.1633988082709
46	24.22846644803992	25.778434875607008	25.606126339433143	24.386972336919925
47	24.18852390787651	25.898427979344735	25.65143551457612	24.261612598202635
48	24.150335662099554	25.961252300173115	25.771789293663467	24.11662274406386
49	24.298988190869466	25.816222838730308	25.616130502239848	24.26865846816038
50	24.383361901839113	25.954774433557642	25.66252236202909	23.99934130257416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2560.0
1	1843.5
2	1127.0
3	1037.5
4	948.0
5	929.5
6	911.0
7	912.0
8	913.0
9	961.5
10	1010.0
11	1197.5
12	1385.0
13	1979.5
14	2574.0
15	3364.0
16	4154.0
17	4549.0
18	4944.0
19	5231.0
20	5518.0
21	6318.0
22	7118.0
23	9384.5
24	11651.0
25	15249.0
26	18847.0
27	24304.0
28	29761.0
29	37725.0
30	45689.0
31	56605.0
32	67521.0
33	81092.0
34	94663.0
35	108804.5
36	122946.0
37	136736.5
38	150527.0
39	164123.0
40	177719.0
41	191130.5
42	204542.0
43	214997.0
44	225452.0
45	235588.0
46	245724.0
47	253343.5
48	260963.0
49	261100.5
50	261238.0
51	257849.5
52	254461.0
53	246331.5
54	238202.0
55	226957.0
56	215712.0
57	200635.5
58	185559.0
59	168609.5
60	151660.0
61	132612.0
62	113564.0
63	97616.5
64	81669.0
65	68547.5
66	55426.0
67	45708.5
68	35991.0
69	29515.0
70	23039.0
71	18490.5
72	13942.0
73	11448.5
74	8955.0
75	6973.5
76	4992.0
77	3994.5
78	2997.0
79	2408.5
80	1820.0
81	1426.5
82	1033.0
83	812.0
84	591.0
85	466.0
86	341.0
87	275.0
88	209.0
89	159.0
90	109.0
91	81.5
92	54.0
93	43.5
94	33.0
95	22.5
96	12.0
97	14.0
98	16.0
99	23.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08557774104696389
2	0.0952759530438916
3	0.1024897465354335
4	0.1036271911523571
5	0.09605420462389197
6	0.05780014619156603
7	0.0536095607607948
8	0.06052402672156733
9	0.060733555993105884
10	0.0449589951215599
11	0.04914958055233114
12	0.05860833052464333
13	0.051514268045409185
14	0.05298097294617911
15	0.05322043497079461
16	0.05546539145156492
17	0.054956534649256976
18	0.05558512246387267
19	0.06432548636233837
20	0.0687555338177251
21	0.08027964375234597
22	0.08285386051696259
23	0.0590573218207974
24	0.07755576322234468
25	0.08476955671388658
26	0.08240486922080853
27	0.07707683917311368
28	0.09572494434004565
29	0.08456002744234802
30	0.08090823156696167
31	0.055794651735411226
32	0.05244218339079425
33	0.055195996673872476
34	0.04974823561386987
35	0.05654297056233466
36	0.05597424825387285
37	0.05911718732695127
38	0.04935910982386969
39	0.05411841756310273
40	0.05561505521694961
41	0.0434324247146361
42	0.03996022535771137
43	0.03993029260463443
44	0.03714654656847926
45	0.029004837731552296
46	0.026430620966935683
47	0.031249794212322598
48	0.025622436633858374
49	0.02586189865847387
50	0.026849679510012804
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3340822.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.898845647154914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.70405553030245	41.93675679188015
2	13.467784252868933	15.32602754826346
3	5.314343259360715	9.071399904910905
4	2.618118876753418	5.9587176741718135
5	1.4247670109441235	4.053379911943399
6	0.8834200070297856	3.015934717295777
7	0.5803534457709157	2.3115008792199676
8	0.39726634541256794	1.8083197174751227
9	0.2988627589791143	1.5304451393551939
>10	1.2507282835277316	11.888815568865727
>50	0.046343164310209004	1.7427380700019452
>100	0.013639169751452813	1.1868727518905613
>500	2.119299925455487E-4	0.08395780705764426
>1k	1.0596499604295171E-4	0.08513351766835198
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.98655061538747E-5
2	0.0	0.0	0.0	0.0	5.98655061538747E-5
3	0.0	0.0	0.0	0.0	5.98655061538747E-5
4	0.0	0.0	0.0	0.0	5.98655061538747E-5
5	0.0	0.0	0.0	0.0	5.98655061538747E-5
6	0.0	0.0	0.0	0.0	5.98655061538747E-5
7	0.0	0.0	0.0	0.0	5.98655061538747E-5
8	0.0	0.0	0.0	0.0	5.98655061538747E-5
9	0.0	0.0	0.0	0.0	5.98655061538747E-5
10	0.0	0.0	0.0	0.0	5.98655061538747E-5
11	0.0	0.0	0.0	2.993275307693735E-5	5.98655061538747E-5
12	0.0	0.0	0.0	2.993275307693735E-5	1.795965184616241E-4
13	0.0	0.0	0.0	2.993275307693735E-5	1.795965184616241E-4
14	0.0	0.0	0.0	2.993275307693735E-5	1.795965184616241E-4
15	0.0	0.0	0.0	2.993275307693735E-5	2.394620246154988E-4
16	0.0	0.0	0.0	2.993275307693735E-5	2.9932753076937355E-4
17	0.0	0.0	0.0	2.993275307693735E-5	2.9932753076937355E-4
18	2.993275307693735E-5	0.0	0.0	2.993275307693735E-5	2.9932753076937355E-4
19	2.993275307693735E-5	0.0	0.0	2.993275307693735E-5	2.9932753076937355E-4
20	2.993275307693735E-5	2.993275307693735E-5	0.0	2.993275307693735E-5	3.292602838463109E-4
21	2.993275307693735E-5	2.993275307693735E-5	0.0	2.993275307693735E-5	3.292602838463109E-4
22	2.993275307693735E-5	2.993275307693735E-5	0.0	5.98655061538747E-5	3.591930369232482E-4
23	2.993275307693735E-5	2.993275307693735E-5	0.0	5.98655061538747E-5	3.891257900001856E-4
24	2.993275307693735E-5	2.993275307693735E-5	0.0	2.0952927153856146E-4	3.891257900001856E-4
25	2.993275307693735E-5	2.993275307693735E-5	0.0	2.394620246154988E-4	3.891257900001856E-4
26	2.993275307693735E-5	2.993275307693735E-5	0.0	5.08856802307935E-4	3.891257900001856E-4
27	2.993275307693735E-5	2.993275307693735E-5	0.0	8.979825923081206E-4	4.190585430771229E-4
28	2.993275307693735E-5	2.993275307693735E-5	0.0	0.0018857634438470532	5.08856802307935E-4
29	2.993275307693735E-5	2.993275307693735E-5	0.0	0.0031728718261553593	5.08856802307935E-4
30	2.993275307693735E-5	2.993275307693735E-5	0.0	0.005028702516925475	5.08856802307935E-4
31	2.993275307693735E-5	2.993275307693735E-5	0.0	0.010326799811543387	5.387895553848723E-4
32	2.993275307693735E-5	2.993275307693735E-5	0.0	0.01772018982154691	5.687223084618097E-4
33	2.993275307693735E-5	2.993275307693735E-5	0.0	0.026400688213858745	5.687223084618097E-4
34	2.993275307693735E-5	2.993275307693735E-5	0.0	0.038703049728479995	5.986550615387471E-4
35	2.993275307693735E-5	5.98655061538747E-5	0.0	0.0521727886131018	5.986550615387471E-4
36	2.993275307693735E-5	5.98655061538747E-5	0.0	0.07165901086618802	5.986550615387471E-4
37	2.993275307693735E-5	8.979825923081205E-5	0.0	0.09294119830389048	5.986550615387471E-4
38	2.993275307693735E-5	8.979825923081205E-5	0.0	0.11679760250620955	6.285878146156844E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4540	0.0	22.200241	1
GGTATCA	2350	0.0	20.882624	1
GGACCGT	355	0.0	16.73046	6
TATCAAC	6030	0.0	16.240177	2
TCAACGC	5985	0.0	15.883808	4
ATCAACG	6105	0.0	15.752767	3
CAACGCA	6120	0.0	15.460126	5
GACCGTA	100	4.95129E-4	15.398218	7
AACGCAG	6285	0.0	15.119967	6
GTACATG	11365	0.0	13.612376	1
TACATGG	11285	0.0	13.474869	2
TTAATAC	670	0.0	13.466977	3
CGTATAC	100	0.008746304	13.204207	3
GTATCGC	135	3.2130466E-4	13.035529	6
ATTATAC	540	0.0	12.633655	3
ACCGTGC	505	0.0	12.632202	8
ACATGGG	11795	0.0	12.631409	3
GTTCGAC	140	4.3702786E-4	12.569033	11
GTGTAGC	1215	0.0	12.497434	1
GTGTAGG	780	0.0	12.413839	1
>>END_MODULE