FastQCFastQC Report
Mon 6 Feb 2017
SRR2077141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3340822
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28800.024.5223921
GTATCAA57250.022.0974771
GGACCGT2850.017.7560486
TATCAAC78450.015.6776942
TCAACGC78350.015.5577614
ATCAACG78900.015.4767343
CAACGCA80500.015.0882555
AACGCAG83350.014.5976466
TATACTG6300.014.3198735
CGTATAC1402.7570755E-514.1441813
ACCGTGC5600.013.75211058
GTACATG104900.013.6748461
TACATGG105650.013.3490952
TATAACG1353.2073676E-413.0382582
CGTTACG1701.1864628E-512.94161224
TACCGTA2054.412159E-712.88156912
ACCGTCG2054.418598E-712.8800258
ATAGGAC4800.012.8345353
GTGGTAT13950.012.61729051
GTATTAG5600.012.5722281
Produced by FastQC (version 0.11.5)