FastQCFastQC Report
Mon 6 Feb 2017
SRR2077140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447465
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT550.00448020720.003923
GTATCAA51400.018.539651
TAACACG800.001985415116.5048964
TAGTCGG1351.0882868E-616.29426840
GGTATCA30100.016.0123231
CGTGCCG3850.015.99708110
GTGCCGA4200.015.71187511
CGCACGA3400.015.52702935
CAACGCA63250.014.5073875
TATCAAC64800.014.3316192
TCAACGC64250.014.2143334
AACGCAG65600.014.0163456
ATCAACG65100.013.9953413
TAGACTG8050.013.9408095
CCCCTAT3650.013.8679311
GTACATG95050.013.7071241
TTAGTCG1453.843286E-513.65387139
GTGTACG3600.013.4492141
TACATGG96350.013.2703462
GTCTACG1505.2623E-513.2046841
Produced by FastQC (version 0.11.5)