##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077140_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3447465
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14844733739139	31.0	30.0	34.0	23.0	34.0
2	29.26092128564032	31.0	30.0	34.0	23.0	34.0
3	29.364874770302237	31.0	30.0	34.0	23.0	34.0
4	32.757883836384124	37.0	35.0	37.0	25.0	37.0
5	32.72918187711841	37.0	35.0	37.0	25.0	37.0
6	32.79038423885377	37.0	35.0	37.0	25.0	37.0
7	32.63735411381987	37.0	33.0	37.0	24.0	37.0
8	32.52657822486958	37.0	33.0	37.0	23.0	37.0
9	33.90808434603397	38.0	34.0	39.0	23.0	39.0
10	33.8667710912221	38.0	34.0	39.0	23.0	39.0
11	33.88286581589661	38.0	34.0	39.0	23.0	39.0
12	33.7966552234758	38.0	34.0	39.0	22.0	39.0
13	33.7419344358826	38.0	34.0	39.0	22.0	39.0
14	34.694341784470616	39.0	34.0	41.0	19.0	41.0
15	34.738426931092846	39.0	34.0	41.0	19.0	41.0
16	34.701312123545854	39.0	34.0	41.0	19.0	41.0
17	34.55134337839543	39.0	34.0	41.0	18.0	41.0
18	34.53066006471422	39.0	34.0	41.0	18.0	41.0
19	34.65844091238055	39.0	34.0	41.0	19.0	41.0
20	34.639911355155164	39.0	34.0	41.0	18.0	41.0
21	34.59247766112201	39.0	34.0	41.0	18.0	41.0
22	34.51650038506555	39.0	34.0	41.0	18.0	41.0
23	34.47804053123092	39.0	34.0	41.0	18.0	41.0
24	34.485417545935924	39.0	34.0	41.0	18.0	41.0
25	34.387436855776635	39.0	34.0	41.0	18.0	41.0
26	34.34162290262555	39.0	34.0	41.0	18.0	41.0
27	34.28488614097605	39.0	34.0	41.0	17.0	41.0
28	34.17540105555821	39.0	34.0	41.0	17.0	41.0
29	34.16823869132826	39.0	34.0	41.0	16.0	41.0
30	34.01114297026946	39.0	33.0	41.0	16.0	41.0
31	34.006246328824226	39.0	33.0	41.0	16.0	41.0
32	33.88454066973849	39.0	33.0	41.0	15.0	41.0
33	33.8386959693572	39.0	33.0	41.0	15.0	41.0
34	33.75678621827923	39.0	33.0	41.0	13.0	41.0
35	33.69289869512816	38.0	33.0	41.0	9.0	41.0
36	33.60572797693378	38.0	33.0	41.0	9.0	41.0
37	33.513270475552325	38.0	33.0	40.0	9.0	41.0
38	33.43098885702973	38.0	33.0	40.0	9.0	41.0
39	33.329413641617826	38.0	33.0	40.0	8.0	41.0
40	33.2651229236555	38.0	33.0	40.0	7.0	41.0
41	33.15837405165825	38.0	33.0	40.0	2.0	41.0
42	33.017707503919546	38.0	32.0	40.0	2.0	41.0
43	32.95034757423208	38.0	32.0	40.0	2.0	41.0
44	32.80230517206121	38.0	32.0	40.0	2.0	41.0
45	32.666199076712886	38.0	32.0	40.0	2.0	41.0
46	32.53656382298298	38.0	32.0	40.0	2.0	41.0
47	32.38427076126951	38.0	32.0	40.0	2.0	41.0
48	32.22656299628858	38.0	32.0	40.0	2.0	41.0
49	32.054964444889215	38.0	31.0	40.0	2.0	41.0
50	31.84531387555784	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4312378682295872
1101	2	0.7281416805995207
1101	3	0.8481813876775419
1101	4	0.8892311446805863
1101	5	0.8794952980154065
1101	6	0.9230432455723161
1101	7	0.9399991109761316
1101	8	0.9524271529482604
1101	9	1.1791395917998315
1101	10	1.211210608305592
1101	11	1.0805081555676495
1101	12	1.1612130612157614
1101	13	1.1170214166149393
1101	14	1.3627059719031678
1101	15	1.371138180513121
1101	16	1.3486373495959327
1101	17	1.482224692068229
1101	18	1.2816980719658702
1101	19	1.3672241482914202
1101	20	1.408886532326946
1101	21	1.467457172253937
1101	22	1.4080774492324934
1101	23	1.5834484797314943
1101	24	1.4581334465397475
1101	25	1.5162821555053725
1101	26	1.4539778295735886
1101	27	1.4396979234671292
1101	28	1.508351931289262
1101	29	1.4297917018336719
1101	30	1.6115669413178182
1101	31	1.6744865122059025
1101	32	1.5525605380776781
1101	33	1.630434062875473
1101	34	1.6232076968432665
1101	35	1.6138459222121568
1101	36	1.7691352742114006
1101	37	1.6557010863564017
1101	38	1.7092733580025978
1101	39	1.854120921834685
1101	40	1.81054553824233
1101	41	1.7679771935229383
1101	42	1.830867112819412
1101	43	1.9906459160861658
1101	44	1.8659169702759897
1101	45	1.8641862204107156
1101	46	1.7395886530445281
1101	47	1.937693209944122
1101	48	1.8996726057092914
1101	49	1.8200431883522583
1101	50	2.020977377233365
1102	1	0.8827521797778104
1102	2	0.9416223262285364
1102	3	1.0720742252937256
1102	4	1.1528283648137716
1102	5	1.1755113326409017
1102	6	1.1538666719395962
1102	7	1.2776324036009044
1102	8	1.176279741527523
1102	9	1.3216377990560346
1102	10	1.4081895180564246
1102	11	1.2876207142764713
1102	12	1.3762693274000029
1102	13	1.3683905608416396
1102	14	1.7091667276151412
1102	15	1.5384631507448319
1102	16	1.6025403368622193
1102	17	1.784208415702338
1102	18	1.7260630714033667
1102	19	1.598969363764084
1102	20	1.6258369649610103
1102	21	1.691643077016593
1102	22	1.705883396686204
1102	23	1.644226494845931
1102	24	1.5599203068416188
1102	25	1.7306646503499223
1102	26	1.6893966620364225
1102	27	1.668783133016909
1102	28	1.8929026769549822
1102	29	1.7947915085890642
1102	30	1.8067262745771373
1102	31	1.8756578453401715
1102	32	1.9586991685617363
1102	33	1.9402833345402186
1102	34	1.9308080689560754
1102	35	1.9279127267512663
1102	36	1.942818853761345
1102	37	2.0083326225476696
1102	38	1.9214283702139312
1102	39	1.984941000269842
1102	40	2.0027958631271403
1102	41	1.8779333774612823
1102	42	1.910144545369043
1102	43	1.7484114569532139
1102	44	1.677110215133652
1102	45	1.7727020057875862
1102	46	1.791837811076526
1102	47	1.7011071554107389
1102	48	1.6161379574427954
1102	49	1.7611252449377233
1102	50	1.7037139129458296
1103	1	0.5479886538886589
1103	2	0.5787863137762379
1103	3	0.5182237263293139
1103	4	0.5406366320589271
1103	5	0.6854124146509903
1103	6	0.6316937773559559
1103	7	0.6231002682037854
1103	8	0.695198662279445
1103	9	0.7809642706413911
1103	10	0.7250967533943609
1103	11	0.7262263844125556
1103	12	0.6144461305163134
1103	13	0.6410843006361446
1103	14	0.8027869534409078
1103	15	0.7890892458973937
1103	16	0.6769616677657453
1103	17	0.8037809203652557
1103	18	0.6573313836427701
1103	19	0.7724419107389195
1103	20	0.7327825200944744
1103	21	0.5404701653928825
1103	22	0.5059590342198206
1103	23	0.7240318074628433
1103	24	0.5854029581499631
1103	25	0.7545356168885817
1103	26	0.66186352314066
1103	27	0.6731965093477186
1103	28	0.8032350251579032
1103	29	0.6612918975857269
1103	30	0.8985113449555655
1103	31	0.7322963190388307
1103	32	0.8859204658259756
1103	33	0.9008591140319737
1103	34	0.6426822207148746
1103	35	0.6830594828107266
1103	36	0.7341077531097611
1103	37	0.7339165647382799
1103	38	0.7673341474819964
1103	39	0.9769536614605769
1103	40	0.7984269266025592
1103	41	0.8907309734729267
1103	42	0.8193367353231835
1103	43	0.6767193337605164
1103	44	0.7136433897202892
1103	45	0.7654081805892972
1103	46	0.7891431073739739
1103	47	0.8199363318305828
1103	48	0.6797212148093834
1103	49	0.6598168139468825
1103	50	0.748009556852935
1104	1	0.5387691744158296
1104	2	0.5650262658712428
1104	3	0.5278043216878707
1104	4	0.46823430862909987
1104	5	0.5137503451759784
1104	6	0.4553024812705715
1104	7	0.5250587504979833
1104	8	0.6406322167884184
1104	9	0.5776605644509729
1104	10	0.4200923070037632
1104	11	0.49306769797898653
1104	12	0.5589901380193467
1104	13	0.557988859898984
1104	14	0.6438260865853209
1104	15	0.5809385739912756
1104	16	0.7070523614961175
1104	17	0.7472048434591301
1104	18	0.60993952833865
1104	19	0.6346034662691267
1104	20	0.6155139006280024
1104	21	0.6138691874894562
1104	22	0.7271572358196821
1104	23	0.6437187807584834
1104	24	0.6826020177697742
1104	25	0.6735597153239823
1104	26	0.6412351142776203
1104	27	0.7478348674508553
1104	28	0.5548356619103458
1104	29	0.6309625326058566
1104	30	0.693983125180921
1104	31	0.7299068504472075
1104	32	0.7900786157618
1104	33	0.6841870281622207
1104	34	0.6923770300639092
1104	35	0.6717217413808569
1104	36	0.6969254545910175
1104	37	0.7534114448181626
1104	38	0.7051702190197844
1104	39	0.8158747007026221
1104	40	0.7151571459455042
1104	41	0.8580204081914502
1104	42	0.871216289058701
1104	43	0.91417299352414
1104	44	0.9189189150239159
1104	45	0.9210999134622284
1104	46	0.7701244942431629
1104	47	0.7267364693127476
1104	48	0.713126618950291
1104	49	0.8051607818696809
1104	50	0.8271415625229679
1105	1	0.45653374195183716
1105	2	0.4503710138603516
1105	3	0.3971486430839555
1105	4	0.39901921541284224
1105	5	0.4291815288884777
1105	6	0.3791964166418538
1105	7	0.4351471754051417
1105	8	0.5060631535720717
1105	9	0.5634844419202523
1105	10	0.5248399610390777
1105	11	0.556881769684999
1105	12	0.5539528833329967
1105	13	0.5131282649855038
1105	14	0.5998912873031514
1105	15	0.5968058152915816
1105	16	0.5289078586104594
1105	17	0.618373562687971
1105	18	0.5452430767186698
1105	19	0.46622668710772075
1105	20	0.5951783412050773
1105	21	0.6645121683244639
1105	22	0.570413493387349
1105	23	0.5596836120895148
1105	24	0.6364178217285286
1105	25	0.5429118196672391
1105	26	0.553146374176734
1105	27	0.5982188980685308
1105	28	0.747798333582935
1105	29	0.6789764401385696
1105	30	0.7051906628746067
1105	31	0.5970221232713868
1105	32	0.5797545644733688
1105	33	0.6793797298707318
1105	34	0.6827099532869738
1105	35	0.7388470351402248
1105	36	0.760005147406531
1105	37	0.8280696624757482
1105	38	0.7764316022051503
1105	39	0.8383920171349359
1105	40	0.7624416756782679
1105	41	0.6709676815062409
1105	42	0.735044532605059
1105	43	0.742603018046367
1105	44	0.738334630113286
1105	45	0.8292224721876877
1105	46	0.7897220177281312
1105	47	0.7336801397168813
1105	48	0.7818818306336368
1105	49	0.7756069622898991
1105	50	0.7727204140612827
1106	1	0.4998195459232697
1106	2	0.44028460441882444
1106	3	0.4154540162344915
1106	4	0.49025319934384015
1106	5	0.5822153159989938
1106	6	0.5937010372865288
1106	7	0.6508891843773696
1106	8	0.6413669593579598
1106	9	0.7678733679917684
1106	10	0.6648938151759367
1106	11	0.5711612608802383
1106	12	0.7024611109297183
1106	13	0.8060909563379113
1106	14	0.8547332787653161
1106	15	0.709651067165062
1106	16	0.7196216653908181
1106	17	0.8989705767027374
1106	18	0.6328442400705256
1106	19	0.7789122434977642
1106	20	0.6764402497293034
1106	21	0.8403910120742495
1106	22	0.8435246481550038
1106	23	0.7291619462780119
1106	24	0.7344471154016574
1106	25	0.7334165733152176
1106	26	0.6762136622576236
1106	27	0.8023116782464044
1106	28	0.6818509016144318
1106	29	0.6647934068456465
1106	30	0.6582446445238119
1106	31	0.6629921597008703
1106	32	0.807798830170043
1106	33	0.6495408652064754
1106	34	0.8073663258231392
1106	35	0.7241394319269148
1106	36	0.7802602392062639
1106	37	0.709607278312177
1106	38	0.8222056356890377
1106	39	0.7889707950541904
1106	40	0.8477224194229933
1106	41	0.8516581333941247
1106	42	0.7178495845343065
1106	43	0.6833840891134457
1106	44	0.882017522166457
1106	45	0.9477449518818091
1106	46	0.8552220888055828
1106	47	0.7882306220979061
1106	48	0.8977096511426268
1106	49	0.9920611795623913
1106	50	0.9806446046171899
1107	1	0.2533498253101989
1107	2	0.19454808745680907
1107	3	0.21309087735332355
1107	4	0.20468213416781111
1107	5	0.12021792389536046
1107	6	0.1293095108121065
1107	7	0.2230092758675042
1107	8	0.2418190659824404
1107	9	0.1921032903961617
1107	10	0.12513942771094122
1107	11	0.08100286324017247
1107	12	0.04573188325181832
1107	13	0.17183880268578378
1107	14	0.042015508730472106
1107	15	0.2081183936437654
1107	16	0.16009536197704222
1107	17	0.11171720460722412
1107	18	0.22185473578459636
1107	19	0.16461049706224173
1107	20	0.16747934679862908
1107	21	0.2000359533965863
1107	22	-0.04919996226375645
1107	23	-0.16601878949077786
1107	24	0.03647144151695869
1107	25	-0.08283682015648708
1107	26	0.26899491960568866
1107	27	0.2292264513380431
1107	28	0.1874322273663438
1107	29	0.31765674880071515
1107	30	0.14355278244305225
1107	31	0.12536684172989965
1107	32	0.1400202448469443
1107	33	0.2505583723140674
1107	34	0.22490122246921374
1107	35	0.27735548117767905
1107	36	0.16821581946320663
1107	37	0.20960941593214955
1107	38	0.16397152354949895
1107	39	0.24480542877300593
1107	40	0.2770291892654555
1107	41	0.32470167671299066
1107	42	0.257237370173776
1107	43	0.13256644947342267
1107	44	0.28974181194222126
1107	45	0.41194836779852295
1107	46	0.299536019674953
1107	47	0.3619389652286671
1107	48	0.3052212477309837
1107	49	0.3299804202797816
1107	50	0.3933078653426989
1108	1	0.15446259717869282
1108	2	0.15633965474250644
1108	3	0.12900319465305543
1108	4	0.06959925837347214
1108	5	0.0948336033438224
1108	6	-0.005321969905338619
1108	7	-0.026925083866785826
1108	8	0.04824958434218729
1108	9	0.04538069574113024
1108	10	-0.005782691380218807
1108	11	0.030400291093855003
1108	12	-0.11932280781476123
1108	13	-0.036957023435562064
1108	14	-0.06423697833677977
1108	15	-0.07675971869965537
1108	16	-0.07478049217034766
1108	17	-0.13256482942928471
1108	18	-0.06789799785324391
1108	19	-0.01375903657755373
1108	20	-0.10918704929139977
1108	21	-0.09765662800222685
1108	22	-0.13346512696558932
1108	23	-0.16834091688232888
1108	24	-0.037534555519307844
1108	25	0.06596682420570943
1108	26	-0.11912690823920968
1108	27	-0.08378658638725511
1108	28	-0.10798378727164959
1108	29	-0.2750958761995932
1108	30	-0.2545425207533327
1108	31	-0.1893874177160697
1108	32	0.014427895615092723
1108	33	-0.013979924249326814
1108	34	-0.05486082470144993
1108	35	0.017303029997435715
1108	36	-0.22025779870391915
1108	37	-0.13115102242642251
1108	38	-0.05148167808421533
1108	39	-0.09732121818429107
1108	40	-0.0775600143603512
1108	41	-0.014014636495140564
1108	42	0.18889102271430858
1108	43	0.1444000985473224
1108	44	0.12799708696608292
1108	45	0.11282592108585021
1108	46	0.16738760859050927
1108	47	0.18829516452606043
1108	48	0.1294475409449234
1108	49	0.08404129758283574
1108	50	0.13040099334701694
1109	1	0.13722024642889252
1109	2	0.13498153043603622
1109	3	0.08348338746059625
1109	4	0.14687938655278998
1109	5	0.10174499038101459
1109	6	0.0501638419673327
1109	7	0.0171422257989704
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2211	3	-0.021707980133960803
2211	4	0.026779675272585735
2211	5	-0.17064820660001345
2211	6	-8.810896212594344E-4
2211	7	0.0040348878666023325
2211	8	0.07768486680262754
2211	9	0.1580387505309062
2211	10	0.0493836812013555
2211	11	0.06881057137736946
2211	12	-0.048603815212494794
2211	13	0.10245204972751765
2211	14	-0.17542572403195322
2211	15	0.0681287626060012
2211	16	-0.042649522713418264
2211	17	0.08432661537378294
2211	18	-0.03750959022833911
2211	19	0.0626414479930304
2211	20	0.03496211261393256
2211	21	0.048741112677873843
2211	22	0.12530067014057522
2211	23	-0.08271664232678688
2211	24	0.020966230089790372
2211	25	0.056837587203425244
2211	26	0.009270540110101422
2211	27	0.08411469144756012
2211	28	0.11364164608123417
2211	29	0.0891591132793721
2211	30	-0.1798718593776485
2211	31	-0.19047743837538889
2211	32	0.07454319882097593
2211	33	0.030269137277933567
2211	34	0.03547154248898465
2211	35	0.10263479434532741
2211	36	0.08951822615232885
2211	37	0.24052939638647075
2211	38	0.18223164540587788
2211	39	0.28044157092895006
2211	40	0.19367619083193688
2211	41	0.23903360574039567
2211	42	0.06954512362249687
2211	43	0.16535886459561056
2211	44	0.00114938652333052
2211	45	0.13515723043114747
2211	46	0.06419053362081684
2211	47	-0.09224000655204634
2211	48	-8.475456969492257E-4
2211	49	0.1590730066223145
2211	50	0.19450827444101293
2212	1	-0.28256988032208596
2212	2	-0.3781900161876912
2212	3	-0.3891008305386059
2212	4	-0.43605392965622514
2212	5	-0.40486444507274655
2212	6	-0.417157939612558
2212	7	-0.41536136107652055
2212	8	-0.24018957600272017
2212	9	-0.3853146865530235
2212	10	-0.4072430232685562
2212	11	-0.4314418935043136
2212	12	-0.4325875564269026
2212	13	-0.38510794682838423
2212	14	-1.0700123706226066
2212	15	-0.5687215438366735
2212	16	-0.5804009009869517
2212	17	-0.46322188157790123
2212	18	-0.4359642372257255
2212	19	-0.39899578145335113
2212	20	-0.3321965674656724
2212	21	-0.5429536543294375
2212	22	-0.5540088826690521
2212	23	-0.5107354994115383
2212	24	-0.5668018168854445
2212	25	-0.28785726578830406
2212	26	-0.5627201631863556
2212	27	-0.7087956485237257
2212	28	-0.6720679563951677
2212	29	-0.5507042476277917
2212	30	-0.5275914517442359
2212	31	-0.4316879514190859
2212	32	-0.37399701653900763
2212	33	-0.39045812918305245
2212	34	-0.4632595529636916
2212	35	-0.4971082792617594
2212	36	-0.537383824968444
2212	37	-0.5260296412395888
2212	38	-0.5922529777536951
2212	39	-0.5153483617653762
2212	40	-0.5277545721496253
2212	41	-0.4477051894780715
2212	42	-0.37708154012727135
2212	43	-0.4934260546230078
2212	44	-0.45944286965050907
2212	45	-0.37560017542668334
2212	46	-0.5015732042191274
2212	47	-0.4847195038129044
2212	48	-0.5001902989958573
2212	49	-0.4375025710533933
2212	50	-0.38629164147484474
2213	1	-0.37704554893167597
2213	2	-0.5056612378111893
2213	3	-0.5320386640540633
2213	4	-0.5887251530180393
2213	5	-0.4253547916365932
2213	6	-0.4589129226608719
2213	7	-0.6029796046608382
2213	8	-0.4356206653379644
2213	9	-0.4357426863854599
2213	10	-0.6202539911291822
2213	11	-0.6243000083574302
2213	12	-0.5861813291859548
2213	13	-0.49606010643682197
2213	14	-0.5852136379617647
2213	15	-0.7962942234258321
2213	16	-0.6569600548478007
2213	17	-0.6295053943170217
2213	18	-0.6145960644822352
2213	19	-0.670811217414105
2213	20	-0.7001157473374136
2213	21	-0.7873985579890146
2213	22	-0.6838320051224258
2213	23	-0.7055828336869325
2213	24	-0.8028809245945752
2213	25	-0.6996855812552596
2213	26	-0.7019902967124168
2213	27	-0.8263726564618281
2213	28	-0.8663569008411969
2213	29	-0.9010475400069211
2213	30	-1.0098207870708364
2213	31	-0.8640190595594888
2213	32	-0.7797811050363421
2213	33	-0.8288923837620459
2213	34	-0.8874764480342066
2213	35	-0.9882529609696533
2213	36	-1.206844746711944
2213	37	-0.9122053718520888
2213	38	-0.8564923101148167
2213	39	-1.0290103616730804
2213	40	-0.9424334606002915
2213	41	-0.9257524545992126
2213	42	-0.8931036298728259
2213	43	-0.9688103253006446
2213	44	-0.9451907881569284
2213	45	-0.9290842864304913
2213	46	-0.9253840486592857
2213	47	-1.0233800322120459
2213	48	-1.0540941188310597
2213	49	-1.0891339825625437
2213	50	-1.0404253714044955
2214	1	-0.4281901066265945
2214	2	-0.6223403297480488
2214	3	-0.613066679915498
2214	4	-0.574911926505294
2214	5	-0.47034079465421996
2214	6	-0.48954565915505555
2214	7	-0.4871549245619491
2214	8	-0.6569939817358978
2214	9	-0.6606816952824204
2214	10	-0.46986592738306143
2214	11	-0.6922489100032223
2214	12	-0.5467099123774375
2214	13	-0.5597405346596886
2214	14	-0.6128582255625616
2214	15	-0.5278599794105716
2214	16	-0.5747971731491575
2214	17	-0.4015429960796908
2214	18	-0.6246405030091822
2214	19	-0.665918702981763
2214	20	-0.6025080270048946
2214	21	-0.5981550651640006
2214	22	-0.5353978904299055
2214	23	-0.5842663752078323
2214	24	-0.7636405252283183
2214	25	-0.7646024818417843
2214	26	-0.7307106437793891
2214	27	-0.7774521853919865
2214	28	-0.7097034148071018
2214	29	-0.5787704193013354
2214	30	-0.6151409189036201
2214	31	-0.5860622236696074
2214	32	-0.6977137458170048
2214	33	-0.7045279940132829
2214	34	-0.7210182408089167
2214	35	-0.8808927481984696
2214	36	-0.7903287924739715
2214	37	-0.6777241963201277
2214	38	-0.7149374892495928
2214	39	-0.7163687551084266
2214	40	-0.6066996326662562
2214	41	-0.6578553114414376
2214	42	-0.39333178164070404
2214	43	-0.5598381486475432
2214	44	-0.41839830515882426
2214	45	-0.3979047057859333
2214	46	-0.44695315674826475
2214	47	-0.41391992673465694
2214	48	-0.3543492520254006
2214	49	-0.3995960894563133
2214	50	-0.40094889925245525
2215	1	-0.2778465705875206
2215	2	-0.37132979743947914
2215	3	-0.3989784062852202
2215	4	-0.3298586656845828
2215	5	-0.3370468638408468
2215	6	-0.3684250095378445
2215	7	-0.32794387387802004
2215	8	-0.28570987907959733
2215	9	-0.39581451024039893
2215	10	-0.39604328366873887
2215	11	-0.47457380850053
2215	12	-0.45924620614344747
2215	13	-0.4132641387167979
2215	14	-0.3369067970339401
2215	15	-0.3120269082613021
2215	16	-0.5057362415717108
2215	17	-0.4197322779949886
2215	18	-0.3802024009222009
2215	19	-0.4489757692169505
2215	20	-0.4341857054024274
2215	21	-0.36198505003223147
2215	22	-0.3316871618578361
2215	23	-0.5345986747868423
2215	24	-0.5235130288614371
2215	25	-0.5753003409215935
2215	26	-0.6263543431216831
2215	27	-0.5760244058663204
2215	28	-0.5493588300317569
2215	29	-0.5030591513791833
2215	30	-0.551843991700899
2215	31	-0.4760108355922412
2215	32	-0.5705824889814934
2215	33	-0.5068391091195821
2215	34	-0.6173130555571404
2215	35	-0.5362670721381235
2215	36	-0.5945193928005779
2215	37	-0.5319441612623734
2215	38	-0.5457593988485243
2215	39	-0.493553197854915
2215	40	-0.5041682829426719
2215	41	-0.6061938135463976
2215	42	-0.5510311538971351
2215	43	-0.6548193463043646
2215	44	-0.6522046185511883
2215	45	-0.6708955518164643
2215	46	-0.7486002719744143
2215	47	-0.6268057493184394
2215	48	-0.7605402418826195
2215	49	-0.8360682904541115
2215	50	-0.7776022319865135
2216	1	-0.7780099928417883
2216	2	-0.7494119892203024
2216	3	-0.7840901582679187
2216	4	-0.7389886825074612
2216	5	-0.7584720020287818
2216	6	-0.9691363769975929
2216	7	-0.8600803795257015
2216	8	-0.9242246591805348
2216	9	-1.0057135729657034
2216	10	-1.0719577273120962
2216	11	-1.210906420853334
2216	12	-1.1492005440429907
2216	13	-0.9521778613796243
2216	14	-1.1856426778318223
2216	15	-1.187333324988053
2216	16	-1.2821049029511826
2216	17	-1.1564205740776572
2216	18	-1.1486258568222212
2216	19	-1.2845801912308872
2216	20	-1.4147264408025677
2216	21	-1.555928813550615
2216	22	-1.3960755300385657
2216	23	-1.4552691866830187
2216	24	-1.580136436696094
2216	25	-1.6778934675738384
2216	26	-1.4615213702786107
2216	27	-1.540047477243391
2216	28	-1.5732280922283408
2216	29	-1.7166280050201337
2216	30	-1.5764534605785698
2216	31	-1.6454508740445348
2216	32	-1.506013875667385
2216	33	-1.595216902919148
2216	34	-1.6696346128750932
2216	35	-1.6473629625490815
2216	36	-1.7993043002747449
2216	37	-1.8423262809451408
2216	38	-1.932913929009544
2216	39	-1.81302688206544
2216	40	-2.0024187035146532
2216	41	-1.8394622586317162
2216	42	-1.7787456456058592
2216	43	-2.0047039894189993
2216	44	-2.1090443205459017
2216	45	-2.2172161478270382
2216	46	-2.407374605067421
2216	47	-2.319314761575111
2216	48	-2.4033929215269367
2216	49	-2.415767881898475
2216	50	-2.4862588049257113
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	170944.0
3	8393.0
4	15857.0
5	12503.0
6	10436.0
7	8894.0
8	7843.0
9	7204.0
10	6916.0
11	6826.0
12	7197.0
13	7533.0
14	8071.0
15	8630.0
16	9116.0
17	9955.0
18	10808.0
19	12186.0
20	15082.0
21	19511.0
22	23711.0
23	27195.0
24	29686.0
25	31915.0
26	35774.0
27	40537.0
28	46222.0
29	53054.0
30	62661.0
31	74718.0
32	91854.0
33	113465.0
34	145198.0
35	189255.0
36	250491.0
37	344236.0
38	542554.0
39	981034.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14850875764303	16.905904728061763	12.894613608974598	25.050972905320606
2	16.37623944632378	18.85076240230255	38.90210119741163	25.87089695396203
3	20.23635269217848	22.959498780605013	27.56329195704547	29.240856570171037
4	13.423616819686327	15.264034045192462	35.49335011279386	35.81899902232735
5	15.176227691176697	36.461201976999654	33.246095112114276	15.116475219709374
6	34.65330787419377	34.52133944329464	16.592933260310996	14.23241942220059
7	30.396932962239564	30.517577133039435	20.15416450211425	18.93132540260675
8	27.130643806520784	34.494054996003385	19.01939795914221	19.35590323833362
9	26.784986081292452	16.106662781251206	18.36134537928224	38.747005758174105
10	15.420874678277738	28.49403654828758	32.42217506160207	23.662913711832616
11	36.395332302129255	22.4229716670391	22.067931778014458	19.11376425281719
12	24.31414968608366	23.96415734824281	28.85897099524482	22.86272197042871
13	28.87829997768261	20.953216766311357	24.93186520294752	25.236618053058514
14	22.867122212274193	20.51859064307968	25.701798045961834	30.912489098684297
15	24.690512813071365	28.228226485184432	22.45076472124677	24.630495980497432
16	24.777832340181497	27.223911334933053	24.111010112619123	23.887246212266326
17	23.660570079600323	27.220251747959495	25.39887955295887	23.720298619481316
18	23.60720834511177	26.811330052978295	25.894121191259945	23.68734041064999
19	24.595353718827397	26.461029584421414	25.783757723943946	23.15985897280725
20	24.64051612184107	25.87764340894459	25.854625062190173	23.627215407024167
21	24.949314203170502	25.65990236742732	25.74585920844229	23.644924220959883
22	24.679966928367634	25.35007971796215	25.60252960828463	24.36742374538559
23	24.358552025325018	25.632552176829304	25.68459186863127	24.324303929214413
24	24.19441410126934	25.603908207403915	25.695261887807696	24.506415803519047
25	24.398044712199475	25.49573129946862	25.782091895507413	24.324132092824495
26	24.27683779173532	25.897863752535756	25.8665986207345	23.95869983499442
27	24.316572825939286	25.79286609204945	25.750832258296747	24.139728823714513
28	24.2820957716643	25.9303197963412	25.636346638050377	24.151237793944127
29	24.353851142701288	25.95342682650327	25.561654310615367	24.131067720180074
30	24.407530794812722	25.958360814878123	25.623531092077883	24.010577298231272
31	24.450221269758714	25.46668647955867	25.555612311235766	24.52747993944685
32	24.16938454850077	25.893969813953515	25.664205046408593	24.272440591137123
33	24.47675866380384	25.616579336429506	25.489458552529616	24.41720344723704
34	24.568563598651906	25.615764547529036	25.559028103258584	24.25664375056047
35	24.60481925472905	25.73219887203992	25.567259669970678	24.095722203260358
36	24.486823732982042	25.739714780016314	25.487418412904063	24.28604307409758
37	24.49603664438697	25.455914326156986	25.731873354890332	24.31617567456571
38	24.550204481529335	25.61930681748268	25.680366871090154	24.150121829897834
39	24.58130164710608	25.832310536945613	25.405623779065532	24.180764036882778
40	24.568188275803422	25.495125438936878	25.80059270808271	24.136093577176997
41	24.326098425468764	25.73904998813109	25.683158743323375	24.251692843076768
42	24.556290714278877	25.679844485927504	25.621140767338563	24.14272403245506
43	24.37161118083617	25.53023367164092	25.57709858317071	24.521056564352197
44	24.47410992671649	25.738979544165247	25.51476189025319	24.272148638865072
45	24.496224257023265	25.62088093512384	25.377211608153726	24.50568319969917
46	24.53973133900505	25.487919307695115	25.41343954895499	24.558909804344836
47	24.43551143601109	25.619270460661202	25.483882574075977	24.461335529251734
48	24.214490575453674	25.80412205124783	25.5974537114274	24.383933661871097
49	24.555211252894175	25.55530409855567	25.47679148605284	24.412693162497316
50	24.590162031831877	25.555621644284347	25.585912848954536	24.26830347492924
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2000.0
1	1508.5
2	1017.0
3	967.5
4	918.0
5	891.5
6	865.0
7	820.0
8	775.0
9	841.5
10	908.0
11	1162.5
12	1417.0
13	2056.5
14	2696.0
15	3610.0
16	4524.0
17	5057.5
18	5591.0
19	6038.0
20	6485.0
21	7732.5
22	8980.0
23	11911.5
24	14843.0
25	19536.5
26	24230.0
27	30746.0
28	37262.0
29	44863.0
30	52464.0
31	62041.5
32	71619.0
33	82664.0
34	93709.0
35	105569.5
36	117430.0
37	128726.0
38	140022.0
39	152494.0
40	164966.0
41	178139.5
42	191313.0
43	201289.5
44	211266.0
45	223162.0
46	235058.0
47	244132.0
48	253206.0
49	256687.0
50	260168.0
51	261170.5
52	262173.0
53	257979.5
54	253786.0
55	245583.5
56	237381.0
57	223986.5
58	210592.0
59	193023.5
60	175455.0
61	155165.5
62	134876.0
63	116111.5
64	97347.0
65	81812.0
66	66277.0
67	54556.0
68	42835.0
69	34707.5
70	26580.0
71	20857.5
72	15135.0
73	12472.5
74	9810.0
75	7421.5
76	5033.0
77	3950.5
78	2868.0
79	2238.5
80	1609.0
81	1226.5
82	844.0
83	658.0
84	472.0
85	380.5
86	289.0
87	219.0
88	149.0
89	120.0
90	91.0
91	63.5
92	36.0
93	31.5
94	27.0
95	21.5
96	16.0
97	15.5
98	15.0
99	26.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0866723810103946
2	0.0953164136546709
3	0.10280017346078932
4	0.10361236444749983
5	0.09444620902605247
6	0.05638925993447359
7	0.05206724361233544
8	0.059492989776545956
9	0.06097233764519727
10	0.04519262704624993
11	0.048006288678782816
12	0.056882375890690694
13	0.049949745682697284
14	0.051632141298026235
15	0.05119703898371702
16	0.05421374836292755
17	0.054416796109605176
18	0.05519998027536176
19	0.06300281511197357
20	0.06923928161707225
21	0.07869550524805909
22	0.0818282419110854
23	0.05853576468506569
24	0.0757658163317104
25	0.0828724874654275
26	0.07927564166713803
27	0.07602687772029593
28	0.09493932498226958
29	0.08307553521210513
30	0.07915961438332224
31	0.055025939349638064
32	0.051255052625624915
33	0.0557801166944407
34	0.04954365018934202
35	0.05665032132305912
36	0.05551905530585517
37	0.058767819252697265
38	0.04864443873976965
39	0.053546591480986755
40	0.055809123515394646
41	0.04313314275851966
42	0.03904318100401309
43	0.040319481125986774
44	0.036548594401973625
45	0.02874575956536179
46	0.025845077469967066
47	0.03106630524167758
48	0.025526002439473644
49	0.02567103654424338
50	0.02668627527763153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3447465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.05254828343457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.52025566531184	45.777686406200196
2	12.387134925639678	14.629837665795117
3	4.281735490119346	7.5854217530150425
4	1.970886978520937	4.655435938412005
5	1.0539752717561357	3.1119962812462636
6	0.6382088850712447	2.2612716600351974
7	0.4238737220799764	1.7521576407444852
8	0.29230136392500417	1.3808912325196072
9	0.2345328290600178	1.2464785090905486
>10	1.0992699331036149	11.575782685686702
>50	0.06734868891477258	2.6877513166995497
>100	0.029288595100653486	2.745024968384242
>500	9.402240235001661E-4	0.40926018713119716
>1k	2.474273732700423E-4	0.18100375503985966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.900682095394732E-5	0.0	0.0	0.0	0.0
3	2.900682095394732E-5	0.0	0.0	0.0	0.0
4	2.900682095394732E-5	0.0	0.0	0.0	0.0
5	2.900682095394732E-5	0.0	0.0	0.0	0.0
6	2.3205456763157855E-4	0.0	0.0	0.0	0.0
7	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
8	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
9	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
10	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
11	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
12	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
13	2.610613885855259E-4	2.900682095394732E-5	0.0	0.0	0.0
14	2.900682095394732E-4	2.900682095394732E-5	0.0	0.0	0.0
15	2.900682095394732E-4	2.900682095394732E-5	0.0	2.900682095394732E-5	0.0
16	3.4808185144736783E-4	2.900682095394732E-5	0.0	5.801364190789464E-5	0.0
17	3.7708867240131516E-4	2.900682095394732E-5	0.0	5.801364190789464E-5	0.0
18	3.7708867240131516E-4	2.900682095394732E-5	0.0	5.801364190789464E-5	0.0
19	4.641091352631571E-4	2.900682095394732E-5	0.0	5.801364190789464E-5	0.0
20	4.641091352631571E-4	2.900682095394732E-5	0.0	5.801364190789464E-5	0.0
21	4.641091352631571E-4	2.900682095394732E-5	0.0	8.702046286184196E-5	0.0
22	4.641091352631571E-4	2.900682095394732E-5	0.0	1.450341047697366E-4	0.0
23	4.641091352631571E-4	2.900682095394732E-5	0.0	2.610613885855259E-4	0.0
24	5.801364190789464E-4	2.900682095394732E-5	0.0	4.931159562171044E-4	0.0
25	5.801364190789464E-4	2.900682095394732E-5	0.0	4.931159562171044E-4	0.0
26	5.801364190789464E-4	2.900682095394732E-5	0.0	8.411978076644723E-4	0.0
27	5.801364190789464E-4	2.900682095394732E-5	0.0	9.862319124342089E-4	0.0
28	6.091432400328938E-4	2.900682095394732E-5	0.0	0.00162438197342105	0.0
29	6.381500609868411E-4	2.900682095394732E-5	0.0	0.002610613885855259	0.0
30	6.381500609868411E-4	2.900682095394732E-5	0.0	0.004380029964046046	0.0
31	6.381500609868411E-4	2.900682095394732E-5	0.0	0.010587489648190772	0.0
32	6.381500609868411E-4	2.900682095394732E-5	0.0	0.01676594251138155	0.0
33	6.671568819407883E-4	2.900682095394732E-5	0.0	0.025177920588026273	0.0
34	6.671568819407883E-4	2.900682095394732E-5	0.0	0.03669362850674336	0.0
35	6.961637028947357E-4	2.900682095394732E-5	0.0	0.05026882071319071	0.0
36	7.25170523848683E-4	2.900682095394732E-5	0.0	0.06720880415029594	0.0
37	7.25170523848683E-4	2.900682095394732E-5	0.0	0.08899292668671038	0.0
38	7.25170523848683E-4	2.900682095394732E-5	0.0	0.11344567675088797	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGT	55	0.004480207	20.0039	23
GTATCAA	5140	0.0	18.53965	1
TAACACG	80	0.0019854151	16.504896	4
TAGTCGG	135	1.0882868E-6	16.294268	40
GGTATCA	3010	0.0	16.012323	1
CGTGCCG	385	0.0	15.997081	10
GTGCCGA	420	0.0	15.711875	11
CGCACGA	340	0.0	15.527029	35
CAACGCA	6325	0.0	14.507387	5
TATCAAC	6480	0.0	14.331619	2
TCAACGC	6425	0.0	14.214333	4
AACGCAG	6560	0.0	14.016345	6
ATCAACG	6510	0.0	13.995341	3
TAGACTG	805	0.0	13.940809	5
CCCCTAT	365	0.0	13.867931	1
GTACATG	9505	0.0	13.707124	1
TTAGTCG	145	3.843286E-5	13.653871	39
GTGTACG	360	0.0	13.449214	1
TACATGG	9635	0.0	13.270346	2
GTCTACG	150	5.2623E-5	13.204684	1
>>END_MODULE