FastQCFastQC Report
Mon 6 Feb 2017
SRR2077140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447465
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60450.018.7056661
GGTATCA34900.017.8388711
CGCACGA3450.017.21349735
GCGATAC2850.016.986514
CGTGCCG3500.016.9762110
CCGTCCG2002.1129381E-814.3034069
CGAATCG3550.014.24967837
CGCGATC1707.591643E-714.23703223
TGCGATA3100.014.19734113
ATACCGT3100.014.1942516
TACCGAT4550.014.0236897
TATACTG7350.013.7701875
TATCAAC81500.013.7129652
TATAGCG1453.835962E-513.656645
CGATACT3750.013.49654715
ACTATAC4600.013.3917723
ATCGTTG4800.013.29348122
TCAACGC82500.013.2808494
GTACATG88550.013.2417371
CAACGCA82750.013.24130255
Produced by FastQC (version 0.11.5)