FastQCFastQC Report
Mon 6 Feb 2017
SRR2077139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989572
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC32490.1086777639073419No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC30960.10355997447126211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49950.021.4114741
GGTATCA24850.021.0763951
TTTACGC1158.80209E-921.03958141
ATAGGCC10850.017.8415056
CTTATAG12700.016.6340793
TATAGGC11850.016.1540435
TCAACGC64800.016.0284294
TATCATA8250.016.0042
ATCAACG65600.016.000953
TCGCGCA1259.440595E-615.84157218
TATCAAC67450.015.692582
CAACGCA66550.015.638445
GGATACG1004.9360783E-415.4043661
GTACATG98200.015.0816671
AACGCAG69100.015.0578056
TTATAGG12800.014.78469854
TACATGG99150.014.603752
ATCATAC13500.014.507333
TCATACT9650.014.3660434
CGTGCGC1707.6141805E-714.23313410
Produced by FastQC (version 0.11.5)