FastQCFastQC Report
Mon 6 Feb 2017
SRR2077139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2989572
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38070.12734264302716242No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC33280.11132028263577529No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC31900.10670423726205625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.025.0817681
GTATCAA61600.022.859431
GCGTATC1051.7927723E-618.8590281
TATCAAC87350.015.9706112
TCAACGC87550.015.910064
ATCAACG87350.015.8953063
CAACGCA90350.015.4654495
TCGTTAG1158.2205E-515.30510922
AACGCAG93000.015.0008556
CGTCGTA1201.1790864E-414.67083510
CGGTTTA1655.4215525E-714.667397522
ATAGGCC10550.014.1829376
ACCGTCG1402.7523713E-514.1466398
GTACATG94100.014.1459431
TACCGCG1100.00101758814.00186917
CGTTAGA1100.001018231714.00069823
TACATGG93600.013.9638692
TATAGGC11150.013.6173085
CGTATCA1302.3290131E-413.5407232
ATATCGA12350.013.53505934
Produced by FastQC (version 0.11.5)