FastQCFastQC Report
Mon 6 Feb 2017
SRR2077138_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077138_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018520
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46750.019.5815031
GCACCGT4600.016.7364946
GGTATCA26600.015.9665091
ATCAACG58050.015.3527833
TATCAAC58900.015.2804142
TCAACGC57950.015.2653554
CAACGCA58600.015.056235
GTACTAG2355.0931703E-1114.98257351
AACGCAG60900.014.4837626
GTACATG90200.014.271961
TACATGG89700.013.7134422
TTAATAC5650.013.6318223
GCTATAC4100.013.4180833
GTATATA6900.013.3947471
ACCGCGC1658.736028E-613.3316768
GTGTAGA8150.012.9603861
GTATAGG4250.012.9445021
CCTATAC3253.6379788E-1212.8648433
GTCTTAG6400.012.7220191
ACATGGG96000.012.6990973
Produced by FastQC (version 0.11.5)