##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077138_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3018520
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96631163616607	31.0	30.0	34.0	23.0	34.0
2	29.099740270066125	31.0	30.0	34.0	22.0	34.0
3	29.244314100950135	31.0	30.0	34.0	23.0	34.0
4	32.644210739037675	35.0	33.0	37.0	25.0	37.0
5	32.660083749652145	35.0	33.0	37.0	25.0	37.0
6	32.73306885493553	37.0	35.0	37.0	25.0	37.0
7	32.54348554920955	36.0	33.0	37.0	23.0	37.0
8	32.502771556921935	36.0	33.0	37.0	23.0	37.0
9	33.84292235930191	38.0	34.0	39.0	23.0	39.0
10	33.77856300438626	38.0	34.0	39.0	22.0	39.0
11	33.83541536912129	38.0	34.0	39.0	23.0	39.0
12	33.724355313199844	38.0	34.0	39.0	22.0	39.0
13	33.669993573009286	38.0	34.0	39.0	21.0	39.0
14	34.603043875806684	39.0	34.0	41.0	19.0	41.0
15	34.64087532963174	39.0	34.0	41.0	19.0	41.0
16	34.615990286630534	39.0	34.0	41.0	19.0	41.0
17	34.46014669440653	39.0	34.0	41.0	18.0	41.0
18	34.44271530418881	39.0	34.0	41.0	18.0	41.0
19	34.56788790533109	39.0	34.0	41.0	19.0	41.0
20	34.53991492519513	39.0	34.0	41.0	18.0	41.0
21	34.500481030438756	39.0	34.0	41.0	18.0	41.0
22	34.43199581251739	39.0	34.0	41.0	18.0	41.0
23	34.39277327962048	39.0	34.0	41.0	18.0	41.0
24	34.404518108211974	39.0	34.0	41.0	18.0	41.0
25	34.30628718709831	39.0	34.0	41.0	18.0	41.0
26	34.25523037780104	39.0	34.0	41.0	18.0	41.0
27	34.187518055205864	39.0	34.0	41.0	17.0	41.0
28	34.08486079270636	39.0	34.0	41.0	17.0	41.0
29	34.069334309529175	39.0	34.0	41.0	16.0	41.0
30	33.91581834806461	39.0	33.0	41.0	16.0	41.0
31	33.899971509216435	39.0	33.0	41.0	16.0	41.0
32	33.77401342379709	38.0	33.0	41.0	15.0	41.0
33	33.73148165326054	38.0	33.0	40.0	15.0	41.0
34	33.657661370472944	38.0	33.0	40.0	13.0	41.0
35	33.60120091965599	38.0	33.0	40.0	9.0	41.0
36	33.5143063488067	38.0	33.0	40.0	9.0	41.0
37	33.42174641877477	38.0	33.0	40.0	9.0	41.0
38	33.34020877781164	38.0	33.0	40.0	9.0	41.0
39	33.22391635636007	38.0	33.0	40.0	8.0	41.0
40	33.16546552615189	38.0	33.0	40.0	8.0	41.0
41	33.055234353259216	38.0	32.0	40.0	2.0	41.0
42	32.914358361051114	38.0	32.0	40.0	2.0	41.0
43	32.842333328916155	38.0	32.0	40.0	2.0	41.0
44	32.69071929289851	38.0	32.0	40.0	2.0	41.0
45	32.555069040456914	38.0	32.0	40.0	2.0	41.0
46	32.416507427481015	38.0	32.0	40.0	2.0	41.0
47	32.257395677351816	38.0	31.0	40.0	2.0	41.0
48	32.097024700846774	38.0	31.0	40.0	2.0	41.0
49	31.92380669997217	38.0	31.0	40.0	2.0	41.0
50	31.71711401614036	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.4717753835416616
1101	2	1.7786578777045676
1101	3	2.055216138774572
1101	4	2.2785498742902135
1101	5	2.4061021108118794
1101	6	2.4208217361791853
1101	7	2.4334061564706673
1101	8	2.5541875367927744
1101	9	2.8955151728872437
1101	10	2.7749078684433073
1101	11	2.816294839726403
1101	12	2.74148229941472
1101	13	2.7719058482921355
1101	14	3.2317765593016645
1101	15	3.2086159308042213
1101	16	3.3438339779949118
1101	17	3.4330734500214604
1101	18	3.387274790774214
1101	19	3.3472636759339167
1101	20	3.369495762807354
1101	21	3.3952502622710057
1101	22	3.3026961463597857
1101	23	3.4183976502986013
1101	24	3.4164968539654197
1101	25	3.386129781199415
1101	26	3.4057117856768997
1101	27	3.484781641643906
1101	28	3.5487430926595707
1101	29	3.520257166471964
1101	30	3.5380996803916247
1101	31	3.564669730791408
1101	32	3.5506507771596745
1101	33	3.6159188011835326
1101	34	3.687572595436066
1101	35	3.70695256584078
1101	36	3.690868803085486
1101	37	3.6946180571988947
1101	38	3.8137074468763217
1101	39	3.8106243362226877
1101	40	3.7981985480046703
1101	41	3.827068737678246
1101	42	3.7523181263401995
1101	43	3.8086933135338015
1101	44	3.8141217614163736
1101	45	4.055294416336409
1101	46	4.026801430004852
1101	47	4.061320289869855
1101	48	3.9800425641843944
1101	49	3.9558236657593433
1101	50	4.086792166815631
1102	1	0.8905478252829298
1102	2	0.9676249341283771
1102	3	1.1734531958874221
1102	4	1.333797098123732
1102	5	1.3302048606139252
1102	6	1.4517031522387
1102	7	1.56293960607794
1102	8	1.616495576065283
1102	9	1.7257373883765013
1102	10	1.8102955918507746
1102	11	1.6597325866639494
1102	12	1.6637870221597026
1102	13	1.8289087466700522
1102	14	2.039450867176086
1102	15	1.9370188778223678
1102	16	2.0828950977204173
1102	17	2.1768700693461653
1102	18	2.144499925817641
1102	19	2.1628501661991137
1102	20	2.1909427820130816
1102	21	2.1338257127021976
1102	22	2.09728645991661
1102	23	2.001836824190036
1102	24	2.029851725180393
1102	25	2.0503676941588154
1102	26	2.1725371765295023
1102	27	1.9696988530697723
1102	28	2.200530751495741
1102	29	2.1633988041882475
1102	30	2.1785930643590206
1102	31	2.113289682310814
1102	32	2.3037169769061805
1102	33	2.181399125102061
1102	34	2.207255599910283
1102	35	2.269150488580344
1102	36	2.2381487862617675
1102	37	2.1867217570003774
1102	38	2.1187391208544923
1102	39	2.3703991251076317
1102	40	2.411078663842048
1102	41	2.322488557774541
1102	42	2.2628066446554627
1102	43	2.292702715140038
1102	44	2.150765025061908
1102	45	2.078819608181071
1102	46	2.280706616737895
1102	47	2.1861404749470736
1102	48	2.15990488919725
1102	49	2.2494834457445556
1102	50	2.2091698336189616
1103	1	0.5080417163691173
1103	2	0.6199707964595262
1103	3	0.5036219041156293
1103	4	0.6132236836435183
1103	5	0.6800512923622577
1103	6	0.5676672748615914
1103	7	0.6436200315622287
1103	8	0.7215212779345208
1103	9	0.7741816738997187
1103	10	0.74473421626017
1103	11	0.7115315052110631
1103	12	0.7444606237362947
1103	13	0.773819643116191
1103	14	0.834261573492995
1103	15	0.7134367266173101
1103	16	0.6923251012597476
1103	17	0.8013818187504356
1103	18	0.7001281180795758
1103	19	0.8042069592172538
1103	20	0.784180259156777
1103	21	0.6709080402673564
1103	22	0.7219098181223274
1103	23	0.6928443696122386
1103	24	0.7192718780298577
1103	25	0.7375859994580196
1103	26	0.8287714531088497
1103	27	0.7255535384102885
1103	28	0.7701205072635346
1103	29	0.7555230745229622
1103	30	0.9370549373670087
1103	31	0.787627331083371
1103	32	0.8503154712185292
1103	33	0.9115558839096778
1103	34	0.920387770510402
1103	35	0.6740832943194661
1103	36	0.8347132975137868
1103	37	0.8478484721137534
1103	38	0.7857384909319975
1103	39	0.904353979954017
1103	40	0.9494875590635274
1103	41	0.8711530280527882
1103	42	0.8438143144244279
1103	43	0.9430027699435968
1103	44	0.856969109640751
1103	45	0.8045440114758406
1103	46	0.7158340008975159
1103	47	0.8247117488432636
1103	48	0.7450369287783403
1103	49	0.6338797770904598
1103	50	0.8323231704643383
1104	1	0.5728464269097984
1104	2	0.6227489994206472
1104	3	0.548396925436144
1104	4	0.4988911635315034
1104	5	0.5688697404286742
1104	6	0.49200532785695117
1104	7	0.5853270144942329
1104	8	0.6447513581372135
1104	9	0.7454232879515317
1104	10	0.46400964292213587
1104	11	0.6547356411915501
1104	12	0.6497806700267006
1104	13	0.6037879781684481
1104	14	0.8211712148827885
1104	15	0.7259949926003273
1104	16	0.7518171353546279
1104	17	0.7723791110306024
1104	18	0.6400812726057552
1104	19	0.7625434338305084
1104	20	0.7211049659981512
1104	21	0.7870075038932782
1104	22	0.783424032657237
1104	23	0.7206438719319621
1104	24	0.6752450101655896
1104	25	0.7766833635666686
1104	26	0.7053829931686124
1104	27	0.8802503420722942
1104	28	0.6799303627342042
1104	29	0.7922065818345487
1104	30	0.8598172735857474
1104	31	0.8845683717342041
1104	32	0.8444091457511291
1104	33	0.8457340755833442
1104	34	0.8484771674870402
1104	35	0.9530004365357172
1104	36	0.9797603854055694
1104	37	0.8767548049425358
1104	38	0.8057496074668364
1104	39	0.8410624567391309
1104	40	0.8488409926961253
1104	41	0.9352037891055858
1104	42	0.8462072293553575
1104	43	0.8615183918565208
1104	44	0.8580317718503139
1104	45	1.015661121691231
1104	46	0.8908761448166231
1104	47	0.8206277041845382
1104	48	0.9633808443116294
1104	49	0.9640986953208106
1104	50	1.101434654044514
1105	1	0.557971799614684
1105	2	0.4775204883582802
1105	3	0.356701429004719
1105	4	0.4941730894148577
1105	5	0.4826727668789843
1105	6	0.30694144969513104
1105	7	0.33725484424058294
1105	8	0.384740219807135
1105	9	0.40922794646540694
1105	10	0.3193438512564555
1105	11	0.4375522685745139
1105	12	0.40347809994972295
1105	13	0.42037816620231894
1105	14	0.626475864732619
1105	15	0.523194895401879
1105	16	0.47701728477911587
1105	17	0.5239144798628672
1105	18	0.4289830389015066
1105	19	0.43104122208215045
1105	20	0.48767796699230104
1105	21	0.5614952474989181
1105	22	0.5440629311392584
1105	23	0.5464583220212447
1105	24	0.46749871263274656
1105	25	0.45670791302645597
1105	26	0.5077056412542049
1105	27	0.59010740151286
1105	28	0.5483587580610987
1105	29	0.47696596932311053
1105	30	0.560102219794139
1105	31	0.47201961635393275
1105	32	0.6419729785778543
1105	33	0.6416864773562949
1105	34	0.5234698030520804
1105	35	0.5460645693800501
1105	36	0.5338664759846949
1105	37	0.5352312393551415
1105	38	0.5862497120398018
1105	39	0.673507557949236
1105	40	0.6888801461024485
1105	41	0.6753069758205044
1105	42	0.5510847218287225
1105	43	0.6043407173576938
1105	44	0.6549253302767823
1105	45	0.6444455244829044
1105	46	0.7588306372428377
1105	47	0.5382140554586172
1105	48	0.5142072527925592
1105	49	0.5268754358762955
1105	50	0.6341518867417228
1106	1	0.2912969486068917
1106	2	0.1657037081205921
1106	3	0.12365672655866433
1106	4	0.14312982507446037
1106	5	0.12319123426013334
1106	6	0.06829220079443843
1106	7	0.1751131350312889
1106	8	0.33030090563366343
1106	9	0.1814116827978225
1106	10	0.24515752888482467
1106	11	0.1271747897554789
1106	12	0.16649678256841582
1106	13	0.3110338275874298
1106	14	0.3470874103932715
1106	15	0.3272008646563904
1106	16	0.22239988146860412
1106	17	0.34744807445737536
1106	18	0.06754029368217118
1106	19	0.19706015660847953
1106	20	0.07613382097515142
1106	21	0.292431738074697
1106	22	0.3388429424272701
1106	23	0.3289486682188709
1106	24	0.36910956634455516
1106	25	0.3610929395086444
1106	26	0.21007795756091951
1106	27	0.32713049092093627
1106	28	0.4076150087187713
1106	29	0.3160130090894384
1106	30	0.3561663223173639
1106	31	0.31524433710315236
1106	32	0.3998760228338796
1106	33	0.30302590855521316
1106	34	0.4047780965531871
1106	35	0.35657756813326813
1106	36	0.4663164053548954
1106	37	0.40515577866707986
1106	38	0.43784924585122553
1106	39	0.35378494333923527
1106	40	0.4069294083956976
1106	41	0.29967696756176565
1106	42	0.3533365051116206
1106	43	0.38298636302249633
1106	44	0.3216174002879697
1106	45	0.5061505406336906
1106	46	0.42477368060603027
1106	47	0.4228185923471486
1106	48	0.49503006605884536
1106	49	0.40811421562292693
1106	50	0.3939719003255995
1107	1	-0.003804044127011963
1107	2	-0.14751486527294233
1107	3	-0.21685611120509307
1107	4	-0.3094418391502387
1107	5	-0.20928772247221872
1107	6	-0.18640328854563393
1107	7	-0.14027392902796265
1107	8	-0.09337706883034969
1107	9	-0.16188948137205728
1107	10	-0.2285922717710136
1107	11	-0.16995733306045935
1107	12	-0.3008469066939128
1107	13	-0.14518708062455232
1107	14	-0.31030258900869967
1107	15	-0.16996595650199708
1107	16	-0.29266306144919696
1107	17	-0.18250556556694875
1107	18	-0.10596200874284989
1107	19	-0.19617640759528854
1107	20	-0.1762019318978929
1107	21	-0.16049376202995802
1107	22	-0.465855225599924
1107	23	-0.5430652185935116
1107	24	-0.3207180881720859
1107	25	-0.28490734455677824
1107	26	-0.14725952711449963
1107	27	-0.14772691571123886
1107	28	-0.19680831355096018
1107	29	-0.08157268398036166
1107	30	-0.15849980113647177
1107	31	-0.1580989883560875
1107	32	-0.18244657332476066
1107	33	-0.14413916834179474
1107	34	-0.13455001396321364
1107	35	-0.1153977580436063
1107	36	-0.12407464558750547
1107	37	-0.2526942696738743
1107	38	-0.23238038615946266
1107	39	-0.11283550040086254
1107	40	-0.10442915941619901
1107	41	-0.0033619718276440835
1107	42	-0.0039252910789144835
1107	43	-0.1867585512096852
1107	44	0.024670293768423335
1107	45	-0.03567497840865741
1107	46	-0.06098598260201982
1107	47	-0.13260749333097266
1107	48	-0.37501716604752744
1107	49	-0.2780143588670043
1107	50	-0.18553589403096638
1108	1	0.10527777013651374
1108	2	0.11041775379413821
1108	3	0.13244030495439318
1108	4	0.046977113242739676
1108	5	0.05441618524661607
1108	6	0.01602398745814071
1108	7	-0.076564107278692
1108	8	0.016052156016748143
1108	9	0.00835393516641858
1108	10	0.07332231043214676
1108	11	0.023471327631490624
1108	12	-0.09431131475829346
1108	13	-0.07985608689403989
1108	14	-0.08331830794424633
1108	15	0.029999488220703086
1108	16	7.614036871288477E-4
1108	17	-0.024657693627069932
1108	18	0.04669846893741436
1108	19	0.048509402571148996
1108	20	-0.025857757001126913
1108	21	0.007897726968089103
1108	22	-0.0790338218877693
1108	23	-0.2972535751990222
1108	24	0.004323759011846562
1108	25	0.027420807962045046
1108	26	-0.00828554017862615
1108	27	-0.004359438915294334
1108	28	-0.18075234019444508
1108	29	-0.20553819310716648
1108	30	-0.10705067789922396
1108	31	-0.02830196655034456
1108	32	0.05163005455082015
1108	33	-0.02862669747845814
1108	34	0.05551144241167805
1108	35	0.13777925291252302
1108	36	-0.17161826388421275
1108	37	5.420630215269284E-5
1108	38	0.12146557595111318
1108	39	0.07835689818125502
1108	40	0.15262765676706636
1108	41	0.17627275473979154
1108	42	0.2548112955285049
1108	43	0.09175032664794003
1108	44	0.04042398585736606
1108	45	0.10237815373616854
1108	46	0.19605984153515266
1108	47	0.15410926919872026
1108	48	0.025484922750862893
1108	49	0.0014547748482947043
1108	50	0.11912909936761551
1109	1	-0.07506362405148082
1109	2	-0.10611402534359371
1109	3	-0.17363420800574758
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2210	41	-0.3899358242635813
2210	42	-0.3396177016674784
2210	43	-0.30222344372822363
2210	44	-0.3573431062128023
2210	45	-0.23646841687669706
2210	46	-0.3282584645319915
2210	47	-0.1884740332727759
2210	48	-0.2622166638415777
2210	49	-0.17373399183424354
2210	50	-0.012329989590902812
2211	1	-0.2933711093240987
2211	2	-0.42431268892323004
2211	3	-0.5066059399615526
2211	4	-0.5538373494559892
2211	5	-0.7822904779364421
2211	6	-0.5356077066289515
2211	7	-0.596081809344291
2211	8	-0.5552008125254773
2211	9	-0.5726845184229319
2211	10	-0.5394309759244464
2211	11	-0.4958035096592468
2211	12	-0.7554620757579897
2211	13	-0.7145231227193278
2211	14	-1.008366058091454
2211	15	-0.7880623510471096
2211	16	-0.8528143784676914
2211	17	-0.6055757287153369
2211	18	-0.8124971292360357
2211	19	-0.6636704140714045
2211	20	-0.7265184989319593
2211	21	-0.7506819742474065
2211	22	-0.7921798722155273
2211	23	-1.031960308574213
2211	24	-0.6850869000532711
2211	25	-0.6857372530193189
2211	26	-0.9152797961225332
2211	27	-0.8487415336824711
2211	28	-0.7490414849813121
2211	29	-0.7586198226564917
2211	30	-1.100206165169105
2211	31	-0.8991256712130564
2211	32	-0.6087364263666686
2211	33	-0.7980055361106508
2211	34	-0.7635991452914439
2211	35	-0.6163076839418622
2211	36	-0.7792470566965406
2211	37	-0.6746079940651839
2211	38	-0.7965480647197722
2211	39	-0.6149889657474006
2211	40	-0.7705536846045717
2211	41	-0.7034602811868851
2211	42	-0.8289778317103327
2211	43	-0.7884182124588293
2211	44	-0.8879258361275859
2211	45	-0.9188574617513332
2211	46	-0.9163802319326102
2211	47	-0.9818349514541431
2211	48	-0.8501758776734079
2211	49	-0.8215493703870251
2211	50	-0.6740235022257473
2212	1	-0.34135681163657594
2212	2	-0.47330739267617616
2212	3	-0.4252576458365631
2212	4	-0.5124739571631878
2212	5	-0.4257566368397079
2212	6	-0.4537789261923706
2212	7	-0.3565800885098085
2212	8	-0.29777919123586827
2212	9	-0.44481639160625264
2212	10	-0.4900066282465332
2212	11	-0.45116611336008106
2212	12	-0.5552858908391158
2212	13	-0.3908680583936004
2212	14	-1.1858282581837898
2212	15	-0.7304295931745699
2212	16	-0.708615463078857
2212	17	-0.5796801517652099
2212	18	-0.5955937594914147
2212	19	-0.615211358440007
2212	20	-0.5745460877624851
2212	21	-0.5542826296901708
2212	22	-0.6262435183239816
2212	23	-0.7357794713590096
2212	24	-0.6282588626367627
2212	25	-0.39955183880049105
2212	26	-0.6630569772827144
2212	27	-0.5939381566067254
2212	28	-0.6957590204530888
2212	29	-0.677614585851039
2212	30	-0.7748388655814296
2212	31	-0.5585803935514093
2212	32	-0.5313690950768049
2212	33	-0.5248059331805734
2212	34	-0.4209614834864368
2212	35	-0.49777537643854686
2212	36	-0.7079011600496443
2212	37	-0.6800793957180602
2212	38	-0.7959883229341003
2212	39	-0.7359968972389623
2212	40	-0.5928934250825222
2212	41	-0.5471312758628954
2212	42	-0.44103437256553946
2212	43	-0.49471541652383877
2212	44	-0.4302476648683964
2212	45	-0.2986512390077891
2212	46	-0.3085472945464147
2212	47	-0.28155200941169056
2212	48	-0.288060707581252
2212	49	-0.3082299331801437
2212	50	-0.21687301391295932
2213	1	-0.6346727690437497
2213	2	-0.8567000338097088
2213	3	-0.771482147727081
2213	4	-0.9309922977614349
2213	5	-0.6433431598029387
2213	6	-0.7353471835923315
2213	7	-0.9767213328865161
2213	8	-0.779470699104003
2213	9	-0.8389189971180926
2213	10	-0.9330969697855949
2213	11	-0.9617887716353835
2213	12	-0.9862952000836884
2213	13	-0.8547650938404416
2213	14	-0.993185893512873
2213	15	-1.2673041907920037
2213	16	-1.0166864737592505
2213	17	-1.08771525026701
2213	18	-1.069373161833262
2213	19	-1.2336782568339686
2213	20	-1.1961649376409653
2213	21	-1.254251585732078
2213	22	-1.1055452931967054
2213	23	-1.0486463631869114
2213	24	-1.2007316587390306
2213	25	-1.2699441586314748
2213	26	-1.2133043754200301
2213	27	-1.4674638401712627
2213	28	-1.4813544629618605
2213	29	-1.3878179897590854
2213	30	-1.501855533966058
2213	31	-1.305978812498232
2213	32	-1.3592240710298071
2213	33	-1.398275773025773
2213	34	-1.1516578425966415
2213	35	-1.4251656264309176
2213	36	-1.615804044120253
2213	37	-1.3888006075364245
2213	38	-1.2758344131374812
2213	39	-1.450717486729772
2213	40	-1.2310448724447056
2213	41	-1.3866529623367825
2213	42	-1.2115139502196541
2213	43	-1.2959551470931814
2213	44	-1.1851872389868099
2213	45	-1.1873063862577773
2213	46	-1.273133726341161
2213	47	-1.2763139650973656
2213	48	-1.4270184690495533
2213	49	-1.3482908816913515
2213	50	-1.2252599117533087
2214	1	-0.5018809854373387
2214	2	-0.7086134004014149
2214	3	-0.7287722168907536
2214	4	-0.7470260653125003
2214	5	-0.6817501525471243
2214	6	-0.5793760533128633
2214	7	-0.5277998052644861
2214	8	-0.7416379487014062
2214	9	-0.9135064498623109
2214	10	-0.8040331949403239
2214	11	-0.9302171620797637
2214	12	-0.8216305418508298
2214	13	-0.7027782597908256
2214	14	-0.8775436291148893
2214	15	-0.7988509474946994
2214	16	-0.8132044476253597
2214	17	-0.6339363436713086
2214	18	-0.7912741828207857
2214	19	-0.9193896040808625
2214	20	-0.7936372610594233
2214	21	-0.9363250410666453
2214	22	-0.8272313673147877
2214	23	-0.9681272820554767
2214	24	-0.9931489926946995
2214	25	-1.0677562589400154
2214	26	-1.074028987624601
2214	27	-1.1522441955064409
2214	28	-1.041488870423379
2214	29	-0.9511836318012286
2214	30	-0.8788369428197313
2214	31	-0.9312377165884484
2214	32	-1.0495732839217666
2214	33	-1.0606683551563734
2214	34	-1.0651444958063934
2214	35	-1.1426035195555713
2214	36	-1.0688624604479742
2214	37	-0.9745512748254228
2214	38	-1.0477217549531161
2214	39	-1.0406370079385496
2214	40	-1.0258201157703013
2214	41	-0.8905193388514405
2214	42	-0.867750904997564
2214	43	-1.0590943655012666
2214	44	-0.8206826897023909
2214	45	-0.8836785971312047
2214	46	-0.9662424141307291
2214	47	-0.9068769933467777
2214	48	-0.9379175563638888
2214	49	-0.8664103087421644
2214	50	-0.9475853709754354
2215	1	-0.4343116821093531
2215	2	-0.5773533182977282
2215	3	-0.5151886134416195
2215	4	-0.622707394174256
2215	5	-0.5253946319272629
2215	6	-0.6524162435204559
2215	7	-0.5231484867898644
2215	8	-0.5316706649254144
2215	9	-0.581887461063566
2215	10	-0.595651665462249
2215	11	-0.7106075109016601
2215	12	-0.7078542689953622
2215	13	-0.6566644937107426
2215	14	-0.7408190954020597
2215	15	-0.7132782624104905
2215	16	-0.8427254950628082
2215	17	-0.658841013129468
2215	18	-0.5954957634355083
2215	19	-0.6514984508501627
2215	20	-0.6667410265564442
2215	21	-0.7203149951343661
2215	22	-0.6192474857237613
2215	23	-0.7348038967576969
2215	24	-0.8407411865820578
2215	25	-0.8234758107169498
2215	26	-0.8556372869640114
2215	27	-0.8378278939729924
2215	28	-0.7924896845498708
2215	29	-0.7934685847136151
2215	30	-0.8257913107055757
2215	31	-0.7350343965935409
2215	32	-0.7952045342861851
2215	33	-0.7502330674155644
2215	34	-0.8859182830926358
2215	35	-0.9638149916128924
2215	36	-0.8747213639038023
2215	37	-0.8067072847849772
2215	38	-0.8502546601322081
2215	39	-0.8613582765074383
2215	40	-0.8047046544931362
2215	41	-0.9841451386713445
2215	42	-1.0492380571312445
2215	43	-1.0534464007095359
2215	44	-1.015278012686025
2215	45	-0.9458883135258098
2215	46	-0.9529723681552582
2215	47	-1.0138857371085592
2215	48	-1.0457664268443523
2215	49	-1.1643356034581167
2215	50	-1.0028424038838537
2216	1	-0.3294419445955441
2216	2	-0.27055341366684615
2216	3	-0.4005941340829793
2216	4	-0.21520510368107892
2216	5	-0.23379201735212973
2216	6	-0.3830008889001988
2216	7	-0.2397724200160951
2216	8	-0.4013025468599878
2216	9	-0.34382302153498756
2216	10	-0.34724888187611924
2216	11	-0.5513086373532232
2216	12	-0.5399672477516972
2216	13	-0.5840800146501337
2216	14	-0.6547437797562381
2216	15	-0.6005619634244539
2216	16	-0.5364476209865785
2216	17	-0.6237846905511191
2216	18	-0.5605096217604526
2216	19	-0.607082711677009
2216	20	-0.6142625350805346
2216	21	-0.7439088774746381
2216	22	-0.7370526212353923
2216	23	-0.6801439088134273
2216	24	-0.7320016452190643
2216	25	-0.8728389430540986
2216	26	-0.7760241423692378
2216	27	-0.7704251449966222
2216	28	-0.8575259911500481
2216	29	-1.0849072836173548
2216	30	-0.8327985854849587
2216	31	-1.053198920819895
2216	32	-0.8717259937797834
2216	33	-0.8388439085384931
2216	34	-0.8738761293656907
2216	35	-0.9169389551226672
2216	36	-1.0089979814534473
2216	37	-1.1172182450231674
2216	38	-1.2500914895320463
2216	39	-1.1602348292599203
2216	40	-1.3804978669422638
2216	41	-1.190108784963435
2216	42	-1.268328176140752
2216	43	-1.3554011792118779
2216	44	-1.4959717749496555
2216	45	-1.5045476515756846
2216	46	-1.6502089470188857
2216	47	-1.5508135103838647
2216	48	-1.6547317728602415
2216	49	-1.6300243521133844
2216	50	-1.7071712196820563
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	148277.0
3	7232.0
4	13659.0
5	10531.0
6	8862.0
7	7748.0
8	6813.0
9	6235.0
10	6223.0
11	6161.0
12	6469.0
13	7044.0
14	7432.0
15	7731.0
16	8152.0
17	8920.0
18	9875.0
19	11218.0
20	13539.0
21	17615.0
22	21459.0
23	24081.0
24	26296.0
25	28672.0
26	32338.0
27	36115.0
28	41713.0
29	48180.0
30	57138.0
31	68042.0
32	83338.0
33	104281.0
34	132716.0
35	172182.0
36	226777.0
37	311965.0
38	487594.0
39	795897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.953255100227324	17.253403909356408	12.879284230741685	24.914056759674583
2	16.26125096953239	19.188965220854424	38.96215350097774	25.587630308635447
3	19.992412318839136	23.17807770342646	28.019601951838126	28.80990802589628
4	13.533189271506135	15.532113450678722	35.76898638045323	35.16571089736192
5	15.06658208430094	36.66953950472891	33.363863455410716	14.90001495555944
6	34.01786895195842	34.98086179754908	16.800348455502146	14.200920794990354
7	30.01404430602334	31.002217190535525	20.252439522805172	18.73129898063596
8	27.007959107227506	34.61363026118203	19.18903957012202	19.189371061468442
9	26.810210572166763	16.141976597788666	18.52333624385823	38.524476586186346
10	15.465439089353117	28.640174630805394	32.50940967310418	23.38497660673731
11	36.15105684104461	22.49495361301421	22.299992376558247	19.05399716938293
12	24.171305789638726	24.123373157858797	28.949917360960566	22.75540369154191
13	28.81754923632608	21.2006315030947	24.99203660410968	24.989782656469544
14	22.798020889578776	20.671607481957537	25.94657250412757	30.58379912433612
15	24.623470863118342	28.207317582496987	22.60146017531915	24.567751379065516
16	24.648710643509794	27.301243198538856	24.218024638567496	23.832021519383854
17	23.56244056196025	27.26892130958762	25.539371724381855	23.629266404070275
18	23.591952089361413	26.957888585291016	26.056763114880983	23.39339621046658
19	24.556893877965063	26.53485144930659	25.95799964595269	22.950255026775658
20	24.647601328604075	26.019203692880154	25.840182283577207	23.493012694938567
21	24.797901665259776	25.878898155436183	25.809669819296804	23.51353036000724
22	24.731854839378155	25.545217717391626	25.640310650176378	24.08261679305384
23	24.269445406675803	25.741477070256884	25.785631058949882	24.203446464117427
24	24.191711505733952	25.829490971458053	25.745543086782284	24.23325443602571
25	24.350226380873593	25.693093518262167	25.753074917065522	24.203605183798725
26	24.163330887518953	25.878827083354743	25.929489591805403	24.028352437320898
27	24.275873089970638	25.935619312349644	25.679567294303002	24.108940303376716
28	24.177749715973718	26.062271560297468	25.66602753844552	24.093951185283295
29	24.22840984937733	26.14459073717144	25.66431041394295	23.96268899950828
30	24.396277500482423	26.097383388405582	25.65229615807228	23.854042953039716
31	24.45349014512162	25.747585173595688	25.54418950006298	24.25473518121971
32	24.208846651173907	26.063304200712455	25.623414552654005	24.104434595459633
33	24.307282910194058	25.854941103468025	25.543718940764037	24.294057045573876
34	24.50569971680402	25.82598826911042	25.671562934207316	23.996749079878235
35	24.51521222599961	25.929135131981607	25.540708250905602	24.014944391113186
36	24.387351175974157	25.977868071590667	25.51599068952784	24.11879006290734
37	24.486735423484916	25.635069093276503	25.74820525212988	24.129990231108696
38	24.429102513619473	25.805302213719305	25.7276423896024	24.03795288305882
39	24.475935685298555	25.979754399700617	25.562564699042344	23.981745215958483
40	24.53320834508689	25.745147948980662	25.84243591838683	23.879207787545614
41	24.276446444243522	25.846051560523826	25.731143394255323	24.146358600977333
42	24.392681085402142	25.788740712655578	25.673471254554602	24.145106947387678
43	24.24349383486915	25.57220182865961	25.77973891820878	24.404565418262468
44	24.27193217934824	25.754050713038172	25.702118069709712	24.27189903790387
45	24.400925896776098	25.683693910588328	25.591700730543828	24.323679462091754
46	24.430729159763	25.594621104675053	25.5031944646952	24.471455270866745
47	24.207727254500583	25.84286009226681	25.64077582067324	24.308636832559362
48	24.205645693092897	25.841575168653037	25.735303376639557	24.21747576161451
49	24.413489517420597	25.729482612872427	25.54119482881287	24.315833040894105
50	24.370420126408074	25.75578580548612	25.689145361994136	24.184648706111666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1812.0
1	1269.0
2	726.0
3	737.5
4	749.0
5	688.5
6	628.0
7	642.0
8	656.0
9	711.5
10	767.0
11	883.5
12	1000.0
13	1587.5
14	2175.0
15	2972.5
16	3770.0
17	4224.5
18	4679.0
19	5115.5
20	5552.0
21	6661.0
22	7770.0
23	9938.0
24	12106.0
25	16315.5
26	20525.0
27	26310.0
28	32095.0
29	39782.0
30	47469.0
31	56073.5
32	64678.0
33	75257.5
34	85837.0
35	96697.5
36	107558.0
37	118204.5
38	128851.0
39	139989.0
40	151127.0
41	161851.5
42	172576.0
43	181439.0
44	190302.0
45	201099.0
46	211896.0
47	220316.5
48	228737.0
49	230038.0
50	231339.0
51	230911.0
52	230483.0
53	224164.5
54	217846.0
55	208712.5
56	199579.0
57	187080.0
58	174581.0
59	159551.0
60	144521.0
61	127138.0
62	109755.0
63	94770.5
64	79786.0
65	67189.0
66	54592.0
67	45317.0
68	36042.0
69	29444.5
70	22847.0
71	18136.5
72	13426.0
73	11120.5
74	8815.0
75	6791.0
76	4767.0
77	3700.0
78	2633.0
79	2057.5
80	1482.0
81	1148.5
82	815.0
83	665.0
84	515.0
85	387.5
86	260.0
87	208.5
88	157.0
89	124.5
90	92.0
91	71.5
92	51.0
93	42.0
94	33.0
95	25.0
96	17.0
97	21.5
98	26.0
99	22.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0853398354160317
2	0.09577541311636166
3	0.10289810900706306
4	0.10428951936710705
5	0.09670302002305765
6	0.05893616739329208
7	0.054298132859812095
8	0.06128831347812835
9	0.062348435657209496
10	0.04677789115195526
11	0.050024515325391254
12	0.05916806911996608
13	0.052707949591190385
14	0.053304268316923524
15	0.053734942952175235
16	0.05648463485416694
17	0.057080953579900076
18	0.05754475703324808
19	0.06539628692206777
20	0.07026622318222175
21	0.07997296688443344
22	0.08358400805692856
23	0.0597975166637955
24	0.07828339716152287
25	0.08563799477889827
26	0.08225885533307714
27	0.07738891907292315
28	0.09786252865642765
29	0.08524044896174283
30	0.08222572651498085
31	0.057644143487536936
32	0.05390058704265667
33	0.05645150603607066
34	0.05062083405112439
35	0.057511628215151794
36	0.05638524839987808
37	0.05966500139141036
38	0.05022328823396896
39	0.05522573976650809
40	0.05641837721797437
41	0.04399507043186727
42	0.04114599207558671
43	0.04068218862223871
44	0.03809814081072844
45	0.029981580377138467
46	0.02650305447702848
47	0.032035567099108174
48	0.02603925102368048
49	0.026469925658932192
50	0.027596305474205902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3018520.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68473646640963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59984269040669	38.43818685870869
2	13.841854083812496	14.861925773919396
3	5.753869066417484	9.26684833478547
4	2.958665939659952	6.35340805051147
5	1.6900704071806463	4.5365492209585305
6	1.0290471730510966	3.3146475778051165
7	0.6925920768664742	2.602713618770999
8	0.4979287263692538	2.1384937963350703
9	0.34266211436185645	1.6556152775885082
>10	1.525487200766479	13.562517454880908
>50	0.05284484435499468	1.892853435022268
>100	0.014700087190772796	1.1862581534686258
>500	3.111354013072399E-4	0.11715639926319775
>1k	1.244541604455307E-4	0.07282604798176569
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.31288180962856E-5	0.0	0.0	0.0	0.0
6	6.62576361925712E-5	0.0	0.0	0.0	3.31288180962856E-5
7	9.938645428885679E-5	0.0	0.0	0.0	3.31288180962856E-5
8	1.325152723851424E-4	0.0	0.0	0.0	3.31288180962856E-5
9	1.325152723851424E-4	0.0	0.0	0.0	3.31288180962856E-5
10	1.65644090481428E-4	0.0	0.0	0.0	3.31288180962856E-5
11	1.65644090481428E-4	0.0	0.0	0.0	3.31288180962856E-5
12	1.65644090481428E-4	0.0	0.0	0.0	3.31288180962856E-5
13	1.65644090481428E-4	0.0	0.0	0.0	3.31288180962856E-5
14	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
15	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
16	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
17	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
18	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
19	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
20	1.65644090481428E-4	0.0	0.0	0.0	6.62576361925712E-5
21	1.65644090481428E-4	0.0	0.0	1.325152723851424E-4	9.938645428885679E-5
22	1.65644090481428E-4	0.0	0.0	1.9877290857771358E-4	9.938645428885679E-5
23	1.65644090481428E-4	0.0	0.0	2.3190172667399917E-4	1.325152723851424E-4
24	2.3190172667399917E-4	0.0	0.0	4.3067463525171275E-4	1.325152723851424E-4
25	2.3190172667399917E-4	0.0	0.0	5.300610895405696E-4	1.325152723851424E-4
26	2.3190172667399917E-4	0.0	0.0	7.288339981182831E-4	1.65644090481428E-4
27	2.3190172667399917E-4	0.0	0.0	0.0012920239057551382	1.65644090481428E-4
28	2.981593628665704E-4	0.0	0.0	0.002219630812451135	1.65644090481428E-4
29	3.31288180962856E-4	0.0	0.0	0.004273617534420842	1.65644090481428E-4
30	3.31288180962856E-4	0.0	0.0	0.007453984071664259	1.65644090481428E-4
31	3.31288180962856E-4	0.0	0.0	0.01745888713674251	1.65644090481428E-4
32	3.31288180962856E-4	0.0	0.0	0.028325139472324186	1.65644090481428E-4
33	3.31288180962856E-4	0.0	0.0	0.042404887163245565	1.65644090481428E-4
34	3.31288180962856E-4	0.0	0.0	0.06082451002478036	1.65644090481428E-4
35	3.9754581715542716E-4	0.0	0.0	0.08252388587784742	1.65644090481428E-4
36	3.9754581715542716E-4	0.0	0.0	0.10843062162914276	1.65644090481428E-4
37	3.9754581715542716E-4	0.0	0.0	0.14351403999310922	1.65644090481428E-4
38	4.6380345334799835E-4	0.0	0.0	0.18121463498668222	1.65644090481428E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4675	0.0	19.581503	1
GCACCGT	460	0.0	16.736494	6
GGTATCA	2660	0.0	15.966509	1
ATCAACG	5805	0.0	15.352783	3
TATCAAC	5890	0.0	15.280414	2
TCAACGC	5795	0.0	15.265355	4
CAACGCA	5860	0.0	15.05623	5
GTACTAG	235	5.0931703E-11	14.9825735	1
AACGCAG	6090	0.0	14.483762	6
GTACATG	9020	0.0	14.27196	1
TACATGG	8970	0.0	13.713442	2
TTAATAC	565	0.0	13.631822	3
GCTATAC	410	0.0	13.418083	3
GTATATA	690	0.0	13.394747	1
ACCGCGC	165	8.736028E-6	13.331676	8
GTGTAGA	815	0.0	12.960386	1
GTATAGG	425	0.0	12.944502	1
CCTATAC	325	3.6379788E-12	12.864843	3
GTCTTAG	640	0.0	12.722019	1
ACATGGG	9600	0.0	12.699097	3
>>END_MODULE