FastQCFastQC Report
Mon 6 Feb 2017
SRR2077138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3018520
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.020.1657241
GTATCAA51650.019.765931
TTAGTCG1002.492621E-517.59373939
CGCTATC3400.016.82116533
TAGTCGG953.3583282E-416.20422240
TATCAAC68300.014.7548912
GACGCTA4500.014.66655126
CGCTACG4200.014.66630728
ATCAACG69150.014.3826023
TACCGTA1402.7535096E-514.14602857
TCAACGC70850.014.0998484
TATGCCG4550.014.0160643
TATATCG950.006234796713.897395
ACGCTAC4600.013.86945527
CAACGCA72050.013.8652425
TATCTCG3650.013.85968936
TTATACT7000.013.8309834
GTACATG85050.013.8145451
CCGTATG1302.3272424E-413.5419459
ATTATAC6200.013.4859993
Produced by FastQC (version 0.11.5)