FastQCFastQC Report
Mon 6 Feb 2017
SRR2077137_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077137_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466818
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41850.020.8216321
GCGATAC3550.019.2114714
GGTATCA23700.018.6621951
CGATACT3800.017.94755615
TGCGATA3700.017.83648113
TCATTCG4200.016.2406124
TATCAAC54700.015.9702572
ATTCGGT4600.015.78505326
ACCGCGT1401.5892347E-615.7133168
CTAACGC1004.937937E-415.4037193
CAACGCA57300.015.1297435
TCAACGC57450.015.0913294
ATCAACG58050.015.0113773
CGATCGT2203.0559022E-1015.00232325
ATACCGT2651.8189894E-1214.9418286
CATTCGG5100.014.6689425
AACGCAG59550.014.4804296
CGCGATC2451.0004442E-1014.36936423
GTGCGAT4950.014.22072912
GTACATG98700.014.20161
Produced by FastQC (version 0.11.5)