FastQCFastQC Report
Mon 6 Feb 2017
SRR2077137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466818
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.020.3761841
GTATCAA51500.020.1660231
TGCGATA2900.018.21110713
GCGATAC3400.016.8274214
CGTGCCG4150.016.43682310
CGCGATC1259.447753E-615.84046223
CGATACT3650.015.07197915
CGCACGA4300.014.83443235
GTACCGA5050.014.8133836
TATCAAC69750.014.7951852
CAACGCA71000.014.4419425
TCAACGC71000.014.4417344
GTGCCGA5450.014.13129911
CGAATCG3900.014.09992437
ATCAACG72800.014.0844553
GTACATG98200.014.0488751
AACGCAG74650.013.8232366
TACCGAT4150.013.7847287
TACATGG98550.013.5079832
CGATTAC1150.001422322713.39440114
Produced by FastQC (version 0.11.5)