FastQCFastQC Report
Mon 6 Feb 2017
SRR2077136_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077136_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775720
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76750.016.8301491
GTACTAG3800.013.8981151
GTATAGG5900.013.7999641
TATCAAC93500.013.7445172
TCAACGC93650.013.5574814
GGTATCA48100.013.5417531
ATCAACG95400.013.4477123
CAACGCA94100.013.444815
GTACATG106500.013.4304941
GTATAGA7450.013.29182151
GTATAAT8000.013.203211
TACATGG104250.013.0026822
AGAGTAC87350.012.76768511
AACGCAG99000.012.7311986
GTATTAA10800.012.2251941
ACATGGG109550.012.2129183
GTATAAA10100.012.2009851
TATACCG1455.857525E-412.1394355
TATACAG13050.012.1394345
TATACTG8400.012.0491115
Produced by FastQC (version 0.11.5)