FastQCFastQC Report
Mon 6 Feb 2017
SRR2077136_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077136_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775720
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80650.016.2580911
GGTATCA52000.016.1194151
CGTACAC3100.015.6137023
TAGTCCG850.002978913515.5314415
GTATAGA7750.013.9098371
GTACATG100100.013.8243331
TACATGG100400.013.3013032
TATCAAC104150.012.3998952
ACATGGG106000.012.3911993
CGTATTA1609.6578144E-512.37563328
GTTACGG3409.094947E-1212.29507225
TGTTACG2706.53381E-912.22317224
GGACCGT3850.012.0009336
ATCAACG107850.011.9542513
TCAACGC107400.011.9430414
CAACGCA108400.011.8742465
GTATAAT8400.011.785851
TAATACT9150.011.782184
CGAATCG1507.808737E-411.73098237
TACACTG21200.011.5203735
Produced by FastQC (version 0.11.5)