FastQCFastQC Report
Mon 6 Feb 2017
SRR2077135_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077135_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830006
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA550.004490153419.9960867
GTATCAA38350.019.7990741
GGTATCA21500.019.347041
GTACCGA2900.018.9618056
CGACGCA600.007416817418.33233631
CGAATCG2400.017.41541537
TACCGAT2600.016.9197657
GTATACG1053.7646845E-516.7684081
CGTGCCG2301.8189894E-1216.25682410
ACCGTAT1105.6340872E-515.9971518
GTACGAC850.002973913215.5351633
CAACGCA48950.015.2833145
TATCAAC48900.015.167172
TCAACGC49050.015.1651194
ATCAACG49050.015.0759193
GCACCGT4450.014.8285586
AACGCAG52050.014.4102436
GCGATAC1851.2680357E-714.26974814
GTATAGA4950.014.2277411
GGCGTAA1100.001020099413.997266
Produced by FastQC (version 0.11.5)