##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077135_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2830006
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.04157341009171	31.0	30.0	34.0	23.0	34.0
2	29.084458124823765	31.0	30.0	34.0	22.0	34.0
3	29.21856561434852	31.0	30.0	34.0	22.0	34.0
4	32.634678159692946	35.0	33.0	37.0	25.0	37.0
5	32.5790623765462	35.0	33.0	37.0	24.0	37.0
6	32.62997604952074	36.0	33.0	37.0	25.0	37.0
7	32.502812714884705	36.0	33.0	37.0	23.0	37.0
8	32.428996616968305	37.0	33.0	37.0	22.0	37.0
9	33.78925345034604	38.0	34.0	39.0	22.0	39.0
10	33.74503940981044	38.0	34.0	39.0	22.0	39.0
11	33.74611290576769	38.0	34.0	39.0	22.0	39.0
12	33.66150283780317	38.0	34.0	39.0	21.0	39.0
13	33.59777717785757	38.0	34.0	39.0	19.0	39.0
14	34.54829318383071	39.0	34.0	41.0	18.0	41.0
15	34.58375105918503	39.0	34.0	41.0	18.0	41.0
16	34.55339211294958	39.0	34.0	41.0	18.0	41.0
17	34.392574432704386	39.0	34.0	41.0	17.0	41.0
18	34.363658946306124	39.0	34.0	41.0	17.0	41.0
19	34.489620870061756	39.0	34.0	41.0	18.0	41.0
20	34.482481309226905	39.0	34.0	41.0	18.0	41.0
21	34.42485280949934	39.0	34.0	41.0	18.0	41.0
22	34.347050854309146	39.0	34.0	41.0	18.0	41.0
23	34.30312091211114	39.0	34.0	41.0	18.0	41.0
24	34.31570639779562	39.0	34.0	41.0	18.0	41.0
25	34.21379283294806	39.0	34.0	41.0	17.0	41.0
26	34.167398938376806	39.0	34.0	41.0	17.0	41.0
27	34.110523087230206	39.0	34.0	41.0	16.0	41.0
28	34.00970775326978	39.0	33.0	41.0	16.0	41.0
29	33.994007786555926	39.0	33.0	41.0	16.0	41.0
30	33.829560078671214	39.0	33.0	41.0	15.0	41.0
31	33.830876330297535	39.0	33.0	41.0	15.0	41.0
32	33.69969604304726	39.0	33.0	41.0	10.0	41.0
33	33.666267138656245	38.0	33.0	41.0	10.0	41.0
34	33.58705493910614	38.0	33.0	40.0	9.0	41.0
35	33.51921268011446	38.0	33.0	40.0	9.0	41.0
36	33.43563158523339	38.0	33.0	40.0	9.0	41.0
37	33.33572084299468	38.0	33.0	40.0	8.0	41.0
38	33.246365555408715	38.0	33.0	40.0	8.0	41.0
39	33.13654423347512	38.0	33.0	40.0	7.0	41.0
40	33.072514333891874	38.0	32.0	40.0	2.0	41.0
41	32.96750112897287	38.0	32.0	40.0	2.0	41.0
42	32.823383059965245	38.0	32.0	40.0	2.0	41.0
43	32.735746496650535	38.0	32.0	40.0	2.0	41.0
44	32.58682384418973	38.0	32.0	40.0	2.0	41.0
45	32.442201889324615	38.0	32.0	40.0	2.0	41.0
46	32.30384741233764	38.0	31.0	40.0	2.0	41.0
47	32.15288483487314	38.0	31.0	40.0	2.0	41.0
48	31.994706018291126	38.0	31.0	40.0	2.0	41.0
49	31.822854439177867	38.0	31.0	40.0	2.0	41.0
50	31.610113900818586	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.5246191511265899
1101	2	1.6207653270786828
1101	3	1.7247676992027507
1101	4	2.015832688181142
1101	5	2.00696100817909
1101	6	1.9024549108292135
1101	7	2.0230865697982523
1101	8	2.0535487620948913
1101	9	2.3478343411621694
1101	10	2.3282500161668054
1101	11	2.2895730944064425
1101	12	2.3879681075458166
1101	13	2.4057547442167007
1101	14	2.7335851512480716
1101	15	2.6429216077926583
1101	16	2.748088502408635
1101	17	2.892062535178084
1101	18	2.928728657295892
1101	19	2.7585434152862547
1101	20	2.8241679624015887
1101	21	2.7534585263028077
1101	22	2.7600778216234687
1101	23	2.8879637707184926
1101	24	2.7612240864885607
1101	25	2.8059172641585164
1101	26	2.916310559873452
1101	27	2.847337264260709
1101	28	3.0607705890397128
1101	29	2.9725007806575334
1101	30	3.157898117032097
1101	31	3.087511528614314
1101	32	3.013850004956069
1101	33	3.2103835075559815
1101	34	3.192491345517766
1101	35	3.1737445129551034
1101	36	3.2230247722516268
1101	37	3.133452813781254
1101	38	3.291908023086954
1101	39	3.294030734333866
1101	40	3.304083713096297
1101	41	3.433443361667557
1101	42	3.3507026247521523
1101	43	3.256268326653675
1101	44	3.342117376052272
1101	45	3.3756242961181613
1101	46	3.2976969482287117
1101	47	3.4135384355596585
1101	48	3.3172672962319965
1101	49	3.4658260643056593
1101	50	3.1850764951072534
1102	1	0.8435397127655975
1102	2	0.7822715662548241
1102	3	0.8119976213841298
1102	4	0.9095344556693163
1102	5	0.9783959019440829
1102	6	0.9262409054889957
1102	7	1.1376121017995118
1102	8	1.088064408261019
1102	9	1.1902156204366534
1102	10	1.2122689098944335
1102	11	1.1063093965796327
1102	12	1.1971790332615981
1102	13	1.2768997804102753
1102	14	1.5593394041867867
1102	15	1.3758391756616817
1102	16	1.4966513383501123
1102	17	1.6084267175616844
1102	18	1.6368849382677126
1102	19	1.6653108657430238
1102	20	1.4807612791890818
1102	21	1.5087557282019546
1102	22	1.4721137181253852
1102	23	1.4290027389419322
1102	24	1.3892612776325493
1102	25	1.4736558542272036
1102	26	1.5109029505823415
1102	27	1.346756219990347
1102	28	1.585853919948292
1102	29	1.434592446632692
1102	30	1.4980082047149992
1102	31	1.4964298307364956
1102	32	1.5972127048236189
1102	33	1.6578170520769007
1102	34	1.659874016783938
1102	35	1.5896791716082106
1102	36	1.7525534959551052
1102	37	1.6522921190283242
1102	38	1.724360863306785
1102	39	1.699962091721531
1102	40	1.7944146694466738
1102	41	1.5605040032913067
1102	42	1.6024695561307354
1102	43	1.4923109607545655
1102	44	1.3534365135850024
1102	45	1.3886141828944574
1102	46	1.3745043551332046
1102	47	1.3386654480653135
1102	48	1.2573157411348106
1102	49	1.2702478201837266
1102	50	1.2647332640844127
1103	1	0.10853998066700399
1103	2	0.16226829595028036
1103	3	0.050156769384830824
1103	4	0.1258838282338246
1103	5	0.17480225456557008
1103	6	0.1468320792156277
1103	7	0.04747226651350189
1103	8	0.21904270616634847
1103	9	0.2801088245900658
1103	10	0.30621647092523574
1103	11	0.2107648772904156
1103	12	0.12021809756377166
1103	13	0.26475266248319684
1103	14	0.31885353102963165
1103	15	0.30067696526180043
1103	16	0.09621011889527864
1103	17	0.4161358738537473
1103	18	0.21391861483274255
1103	19	0.36428401439258096
1103	20	0.177057436766205
1103	21	0.09309978967165478
1103	22	0.08915833963329334
1103	23	0.13508484069463833
1103	24	0.14106105777421618
1103	25	0.1860304311409422
1103	26	0.14857112518424032
1103	27	0.09354297815848867
1103	28	0.050181677291000426
1103	29	0.12870580019706068
1103	30	0.40448258117375246
1103	31	0.18973323771988504
1103	32	0.2996966623845694
1103	33	0.36261582806514525
1103	34	0.3349770634558311
1103	35	0.23489725288967378
1103	36	0.38969066511637607
1103	37	0.36686510586196874
1103	38	0.34883724899718516
1103	39	0.33581449012693554
1103	40	0.3135929132399582
1103	41	0.3691287639357057
1103	42	0.417572213232944
1103	43	0.3542107048234655
1103	44	0.21374549551862287
1103	45	0.20637978575267368
1103	46	0.16695578940566236
1103	47	0.31981565081783714
1103	48	0.1682286109854232
1103	49	0.17177917005250265
1103	50	0.20035918609730174
1104	1	0.50932546569544
1104	2	0.5819278825202865
1104	3	0.5405866912156476
1104	4	0.5258150689300862
1104	5	0.5581243494464729
1104	6	0.4708561582578241
1104	7	0.39097798782285764
1104	8	0.5095791173080855
1104	9	0.656083676900046
1104	10	0.33116963815668043
1104	11	0.4987449576950951
1104	12	0.4758508535641326
1104	13	0.509581177241806
1104	14	0.6859525411536751
1104	15	0.6310155494748102
1104	16	0.6527187713804494
1104	17	0.6976590108492005
1104	18	0.5924198564859751
1104	19	0.6598909668805035
1104	20	0.6116130593074729
1104	21	0.6205562403475895
1104	22	0.694551420071285
1104	23	0.7099437166042222
1104	24	0.6840236845606142
1104	25	0.743487195672003
1104	26	0.6547745776291194
1104	27	0.7933026671839016
1104	28	0.6274449145159906
1104	29	0.6679083270581501
1104	30	0.6643454784724625
1104	31	0.763117861013825
1104	32	0.7836424432378308
1104	33	0.6681716991873756
1104	34	0.6312223645343522
1104	35	0.6873447837259405
1104	36	0.7376191924099302
1104	37	0.7997256700425481
1104	38	0.8154627518633788
1104	39	0.9112580896870739
1104	40	0.7536569339089567
1104	41	0.7970353752842811
1104	42	0.6870454314745089
1104	43	0.695172466679054
1104	44	0.7386944266686299
1104	45	0.8301606848378569
1104	46	0.8333521972856204
1104	47	0.7450864086277775
1104	48	0.7133663693165886
1104	49	0.8365663002209303
1104	50	0.8349823356277071
1105	1	0.3989831424959469
1105	2	0.3802784845659879
1105	3	0.3807055526528025
1105	4	0.4680353251780076
1105	5	0.4626541529464703
1105	6	0.4290376759806733
1105	7	0.5184415297449334
1105	8	0.5308514261589607
1105	9	0.5985400080553518
1105	10	0.42547805443677333
1105	11	0.46905575805830324
1105	12	0.49195366883488134
1105	13	0.45893121471299736
1105	14	0.6097193049786895
1105	15	0.5554268251415806
1105	16	0.5435980218909222
1105	17	0.6622248964390991
1105	18	0.5439369522286626
1105	19	0.41339877567331484
1105	20	0.6228909683291235
1105	21	0.6427281404333982
1105	22	0.5627293337779733
1105	23	0.5269886712823961
1105	24	0.5216258416472286
1105	25	0.5511076572230564
1105	26	0.5758488114293243
1105	27	0.6010116796123768
1105	28	0.6769334158450278
1105	29	0.5776391159840273
1105	30	0.6303715648408783
1105	31	0.5168410539789079
1105	32	0.731963321720869
1105	33	0.6442054475081846
1105	34	0.6230097377899284
1105	35	0.5628932477168576
1105	36	0.6031393492876305
1105	37	0.6464134752878579
1105	38	0.6473760049993018
1105	39	0.7885732872416682
1105	40	0.7106792235730524
1105	41	0.6092674097559581
1105	42	0.6565395402662162
1105	43	0.7272797934415465
1105	44	0.7196009342603062
1105	45	0.8029962106295372
1105	46	0.8210812697008265
1105	47	0.6064904308702239
1105	48	0.6968603581567194
1105	49	0.6772802709516554
1105	50	0.7141865426101646
1106	1	0.1606155194203076
1106	2	0.0817353002145147
1106	3	0.12645637969656676
1106	4	0.1582502231005236
1106	5	0.11763228192908315
1106	6	0.11174997870542569
1106	7	0.14581318911587005
1106	8	0.1729357966202869
1106	9	0.16346290971966937
1106	10	0.11572912471152108
1106	11	0.13936453873728283
1106	12	0.10738988987824172
1106	13	0.2571903674639415
1106	14	0.296967845345641
1106	15	0.20638541943633726
1106	16	0.11987922189275935
1106	17	0.3424036516662241
1106	18	-0.13430970510898277
1106	19	0.1371835037151925
1106	20	0.1331633771462606
1106	21	0.3020605219320984
1106	22	0.2095520127733792
1106	23	0.13122939204055228
1106	24	0.10889988689259411
1106	25	0.23155076269633668
1106	26	0.15145361091182252
1106	27	0.30448134062486787
1106	28	0.17221007053242943
1106	29	0.08307687648675
1106	30	0.12740637558488288
1106	31	0.06305503092188758
1106	32	0.1620964009280712
1106	33	0.05824582915391119
1106	34	0.09732925496226841
1106	35	0.018881874949578048
1106	36	0.10369604403513222
1106	37	0.11007100066939302
1106	38	0.14299479172378682
1106	39	0.21517563032936948
1106	40	0.25340196190908415
1106	41	0.23259562923235677
1106	42	0.16958769406979712
1106	43	0.1189483166560592
1106	44	0.1802451004956751
1106	45	0.29694037471703183
1106	46	0.19666509371248964
1106	47	0.1497434873441179
1106	48	0.1764724072076831
1106	49	0.29078088920680045
1106	50	0.32012147749049547
1107	1	-0.24525028511531133
1107	2	-0.2191411624306312
1107	3	-0.2954403951447979
1107	4	-0.19853928876806037
1107	5	-0.3958594723112938
1107	6	-0.308648783946154
1107	7	-0.30031024521374405
1107	8	-0.30523925776991945
1107	9	-0.1791893853319877
1107	10	-0.3681506705251465
1107	11	-0.38209445546058163
1107	12	-0.4685222688033406
1107	13	-0.32343927447423226
1107	14	-0.5450141547122271
1107	15	-0.5067630705769304
1107	16	-0.4828876411888672
1107	17	-0.42055776596824757
1107	18	-0.37969107830830495
1107	19	-0.42319658640079183
1107	20	-0.36675820469403675
1107	21	-0.3410947580121686
1107	22	-0.6425763056555454
1107	23	-0.6617886245767082
1107	24	-0.6285626492822871
1107	25	-0.49733611676477096
1107	26	-0.40466296498357224
1107	27	-0.4412402952789023
1107	28	-0.44402529174910654
1107	29	-0.4130918555970666
1107	30	-0.3467093672753023
1107	31	-0.4957766160154051
1107	32	-0.47497773100317886
1107	33	-0.4920970069280699
1107	34	-0.5343055410319835
1107	35	-0.4835708107326795
1107	36	-0.576541651321854
1107	37	-0.5181535968719331
1107	38	-0.5509328665323991
1107	39	-0.45609733706516664
1107	40	-0.26355429761320437
1107	41	-0.2428441636932277
1107	42	-0.4457515829994989
1107	43	-0.5386748884131052
1107	44	-0.24610199504441255
1107	45	-0.3017392347316843
1107	46	-0.4719555822358217
1107	47	-0.34627250959991684
1107	48	-0.4809527244063787
1107	49	-0.4425679835411671
1107	50	-0.38436847592641854
1108	1	-0.07481598850003479
1108	2	-0.09961354888664431
1108	3	-0.026361094572738608
1108	4	-0.07431541270710795
1108	5	-2.0278104258864005E-4
1108	6	-0.07568215648026211
1108	7	-0.14832478228979795
1108	8	-0.008104242180145604
1108	9	-0.0709487562011688
1108	10	-0.1611867337690569
1108	11	-0.06054239844768006
1108	12	-0.08111584780464653
1108	13	-0.11891302879745069
1108	14	-0.22844367920830422
1108	15	-0.19148803138838844
1108	16	-0.2007771419882971
1108	17	-0.2313364616934095
1108	18	-0.1795263469657442
1108	19	-0.08129536354218914
1108	20	-0.22764390727615336
1108	21	-0.2621666177781279
1108	22	-0.2336906989954315
1108	23	-0.5433871156650127
1108	24	-0.26955941583018017
1108	25	-0.014409497579492836
1108	26	-0.19879723717789233
1108	27	-0.36978085682387274
1108	28	-0.3910890754704539
1108	29	-0.47461211153260763
1108	30	-0.29057640373104476
1108	31	-0.2937482573630703
1108	32	-0.1588521182157976
1108	33	-0.1707020466500495
1108	34	-0.2067025680374499
1108	35	-0.20487141509018159
1108	36	-0.35391893846190214
1108	37	-0.2681397713804685
1108	38	-0.23702177936915803
1108	39	-0.20929689836886212
1108	40	-0.21121514680670117
1108	41	-0.20475383356006915
1108	42	-0.06417438072105597
1108	43	-0.02351945807816236
1108	44	-0.12892544914647175
1108	45	-0.19116417748436731
1108	46	-0.18243951358895316
1108	47	-0.10766557101397467
1108	48	-0.21041599680477674
1108	49	-0.23728561806564485
1108	50	-0.2727299569815784
1109	1	-0.09945668477625702
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2210	38	-0.10687348311080314
2210	39	0.01429098950593044
2210	40	-0.16089691057694466
2210	41	-0.094116140962349
2210	42	-0.08374623312170826
2210	43	-0.1684017916859375
2210	44	-0.12658621334871611
2210	45	0.09864850381882917
2210	46	-0.0018459108505695099
2210	47	0.08340455615520881
2210	48	0.17064042304588156
2210	49	0.23009529479560342
2210	50	0.2568553169394505
2211	1	0.046529826100538685
2211	2	-0.007027257526758035
2211	3	-0.010396076871639792
2211	4	-0.03897450663274782
2211	5	-0.24857227674823434
2211	6	-0.11138022178730722
2211	7	-0.05916915015415469
2211	8	-0.00892136458681847
2211	9	0.008025907212456218
2211	10	-0.006363032875434271
2211	11	0.11995960333329236
2211	12	-0.08548670457655305
2211	13	-0.04606461151963259
2211	14	-0.3079738700230408
2211	15	-0.17353788406020243
2211	16	-0.24641147367821503
2211	17	-0.07878313516540913
2211	18	-0.24905849250330903
2211	19	-0.20319380965820244
2211	20	-0.24535755727458053
2211	21	-0.14174217746836604
2211	22	-0.15296643798925658
2211	23	-0.23327878540855806
2211	24	-0.09639993216659093
2211	25	-0.06729030051205598
2211	26	-0.17701245457443804
2211	27	-0.030991192239824272
2211	28	0.042537466287008385
2211	29	0.03319205902541
2211	30	-0.44492421407949223
2211	31	-0.19559028197327422
2211	32	0.04405245511164679
2211	33	-0.06854292255893313
2211	34	-0.07358256494850934
2211	35	0.04373699118327323
2211	36	0.0015206207843050379
2211	37	0.1677865862469332
2211	38	0.006065620873613398
2211	39	0.12034273279183338
2211	40	-0.027412800656655634
2211	41	0.1495563302746632
2211	42	0.05324075172951126
2211	43	-0.053820557790750456
2211	44	-0.08744623074601776
2211	45	0.11529388888450853
2211	46	0.04182656038644694
2211	47	-0.00609962083768778
2211	48	0.07523320043158321
2211	49	0.11148423383393435
2211	50	0.2000832270205848
2212	1	-0.08090242776222212
2212	2	-0.14349336832993487
2212	3	-0.14663516400212018
2212	4	-0.09980228067287555
2212	5	-0.1056927581630589
2212	6	-0.19314793439544076
2212	7	-0.1814144858117075
2212	8	-0.1121580208568318
2212	9	-0.2839216644944216
2212	10	-0.24840019840171834
2212	11	-0.23761374049763617
2212	12	-0.2554799943659276
2212	13	-0.30464075661114975
2212	14	-0.9009690508717725
2212	15	-0.54039501713698
2212	16	-0.36006506267540317
2212	17	-0.286927768510985
2212	18	-0.34199903494637596
2212	19	-0.27459378604739015
2212	20	-0.264739094408732
2212	21	-0.2906709794565927
2212	22	-0.364472891245768
2212	23	-0.4932254986999993
2212	24	-0.30522594373413625
2212	25	-0.21467834334681157
2212	26	-0.2989484359175094
2212	27	-0.4273493779287776
2212	28	-0.38505554298133404
2212	29	-0.33965967516247986
2212	30	-0.25620816394908275
2212	31	-0.1562743723666955
2212	32	-0.24523578420193815
2212	33	-0.22811519819806136
2212	34	-0.22549699374457077
2212	35	-0.2511906776614694
2212	36	-0.4082675533581721
2212	37	-0.37876849519062716
2212	38	-0.49696959741888236
2212	39	-0.3823270006473578
2212	40	-0.34288723097996154
2212	41	-0.38369291696609764
2212	42	-0.23429800533233447
2212	43	-0.2957122842195403
2212	44	-0.12271604572589467
2212	45	-0.11297321348504141
2212	46	-0.061855278202486375
2212	47	-0.13598630210092466
2212	48	-0.170753817132141
2212	49	-0.1109336626357198
2212	50	-0.026523350565732073
2213	1	-0.6169040791699096
2213	2	-0.6377510139887512
2213	3	-0.6388710311419814
2213	4	-0.8669211329409663
2213	5	-0.48077363733595035
2213	6	-0.5068776436516558
2213	7	-0.600443478797505
2213	8	-0.49699785912168437
2213	9	-0.589454576360616
2213	10	-0.7422958623498914
2213	11	-0.7027401583262716
2213	12	-0.769037173286101
2213	13	-0.7918454706554172
2213	14	-0.8173199503731183
2213	15	-0.8311435285585915
2213	16	-0.6974106872036216
2213	17	-0.8865363713240555
2213	18	-0.7635917080652916
2213	19	-0.920386739291601
2213	20	-0.9169543671453511
2213	21	-0.9967719955578147
2213	22	-0.8088572037295165
2213	23	-1.0033173453118565
2213	24	-1.1337983310624224
2213	25	-0.9406257920986434
2213	26	-0.9343349504566589
2213	27	-0.9977141244799981
2213	28	-1.0687971380176862
2213	29	-1.0773151260483615
2213	30	-1.1705787843139817
2213	31	-1.0742228642255895
2213	32	-0.9946923207830594
2213	33	-1.0807915870842209
2213	34	-0.9970651152933172
2213	35	-1.2370904369293498
2213	36	-1.171611657491816
2213	37	-1.1094906040803636
2213	38	-0.9207608453508342
2213	39	-1.0899285987216416
2213	40	-0.9397065398126543
2213	41	-0.9956911827381347
2213	42	-0.9076371777329086
2213	43	-0.9781279516733008
2213	44	-0.8950871133916642
2213	45	-0.8650515293347887
2213	46	-0.8871438595536816
2213	47	-0.957476306639979
2213	48	-1.0328418197409803
2213	49	-1.0508817357524585
2213	50	-0.9117346306155234
2214	1	-0.2902623825749586
2214	2	-0.5329666143679717
2214	3	-0.5481486426082895
2214	4	-0.6671772941193552
2214	5	-0.36825886778760974
2214	6	-0.3238928638755425
2214	7	-0.26366041485761826
2214	8	-0.405954393450088
2214	9	-0.6530465305178055
2214	10	-0.5026123626791446
2214	11	-0.5739882260256834
2214	12	-0.5215182238971963
2214	13	-0.4387563583227845
2214	14	-0.5265291432202233
2214	15	-0.44705587800260105
2214	16	-0.4388163278867836
2214	17	-0.23461201009242671
2214	18	-0.4858541460098351
2214	19	-0.5472927699375347
2214	20	-0.54232389774225
2214	21	-0.5571520107827084
2214	22	-0.5505395545278873
2214	23	-0.567909892586087
2214	24	-0.7600843909438879
2214	25	-0.6367864100612195
2214	26	-0.6708006734289711
2214	27	-0.7411629833134725
2214	28	-0.621502825869996
2214	29	-0.5130845202750436
2214	30	-0.5941460797469773
2214	31	-0.5553353556440754
2214	32	-0.6280257319532794
2214	33	-0.7286995652089487
2214	34	-0.7742153980747091
2214	35	-0.7020696778329452
2214	36	-0.692053179420796
2214	37	-0.5236836146397152
2214	38	-0.6019872899644412
2214	39	-0.5525026799740047
2214	40	-0.5811848023066446
2214	41	-0.4195351882863463
2214	42	-0.323047946309849
2214	43	-0.4144005613415729
2214	44	-0.41386954218258865
2214	45	-0.35479340666697823
2214	46	-0.29150840703340464
2214	47	-0.22891725413277442
2214	48	-0.3253757838644056
2214	49	-0.3864131826311201
2214	50	-0.33601997128420535
2215	1	-0.3947093735078049
2215	2	-0.5968008979240231
2215	3	-0.5880162451950888
2215	4	-0.39256314278086535
2215	5	-0.3644870959541109
2215	6	-0.546461445632815
2215	7	-0.4854909574737647
2215	8	-0.4384435316207771
2215	9	-0.5190137407683935
2215	10	-0.6014280314620777
2215	11	-0.697224061969024
2215	12	-0.6005609954814659
2215	13	-0.6050971486991159
2215	14	-0.6241956805641067
2215	15	-0.7009811671161046
2215	16	-0.7518117262223711
2215	17	-0.6255941866719823
2215	18	-0.6597924359172609
2215	19	-0.8176116490943812
2215	20	-0.7913918744230415
2215	21	-0.8233541859748286
2215	22	-0.7181052714857046
2215	23	-0.8826119662897938
2215	24	-0.8979808059621206
2215	25	-0.9049432006636451
2215	26	-0.9110076840942511
2215	27	-0.8756826605032515
2215	28	-0.7063574148162388
2215	29	-0.9413880965211021
2215	30	-0.7997243452971858
2215	31	-0.7131684624484222
2215	32	-0.7659658255281627
2215	33	-0.7942418087965493
2215	34	-0.8515900341937908
2215	35	-0.8359733589075375
2215	36	-0.8745164958565255
2215	37	-0.6947127842075815
2215	38	-0.7762807893105474
2215	39	-0.839602475198653
2215	40	-0.9364706784599477
2215	41	-0.9064348329484417
2215	42	-0.8642625185040771
2215	43	-0.9717633528638245
2215	44	-0.9931544728236119
2215	45	-0.8713154630534241
2215	46	-0.8331398525109996
2215	47	-0.9145569009017187
2215	48	-0.9920499444010851
2215	49	-1.0501345442715824
2215	50	-0.9871394269980023
2216	1	-0.021983182418313874
2216	2	-0.016794715862307186
2216	3	-0.014293319313591013
2216	4	0.17437582898526927
2216	5	0.05380272989328461
2216	6	0.01584380102409
2216	7	0.07456090315508845
2216	8	-0.011637302457636167
2216	9	0.013385169029930921
2216	10	-0.04078114739290584
2216	11	-0.1600883926995138
2216	12	-0.0056600924588821044
2216	13	1.4263924635571357E-4
2216	14	-0.0739232528046827
2216	15	-0.10376652936775344
2216	16	-0.22198131587575887
2216	17	-0.1445164472458842
2216	18	-0.05944494026555702
2216	19	-0.13771021863399113
2216	20	-0.2687808610310256
2216	21	-0.39106297161674775
2216	22	-0.14973866050665663
2216	23	-0.30312009741819423
2216	24	-0.3964574919845276
2216	25	-0.5350134112217262
2216	26	-0.32596398035238394
2216	27	-0.39402007841307096
2216	28	-0.4182510146651168
2216	29	-0.513876817938268
2216	30	-0.4411172962749106
2216	31	-0.41359764962996337
2216	32	-0.35068214985089696
2216	33	-0.4763365219071787
2216	34	-0.5898507405679396
2216	35	-0.5407351410094918
2216	36	-0.5308430510007582
2216	37	-0.7835355184942259
2216	38	-0.6917834130069807
2216	39	-0.7713057803937957
2216	40	-0.8740610481815878
2216	41	-0.8027708574411818
2216	42	-0.7577903659935927
2216	43	-0.7785695084309907
2216	44	-0.9240932385210208
2216	45	-1.041054516265767
2216	46	-1.0842294408887234
2216	47	-0.9468865078256279
2216	48	-1.1156139029812735
2216	49	-1.153263477056381
2216	50	-1.284013438052117
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145095.0
3	7224.0
4	13138.0
5	10446.0
6	8661.0
7	7398.0
8	6821.0
9	6211.0
10	6054.0
11	6049.0
12	6257.0
13	6362.0
14	6838.0
15	7348.0
16	7832.0
17	8444.0
18	9026.0
19	10338.0
20	12912.0
21	16506.0
22	20157.0
23	22872.0
24	24543.0
25	26825.0
26	29827.0
27	33396.0
28	38077.0
29	44114.0
30	51692.0
31	62433.0
32	77146.0
33	95092.0
34	121974.0
35	159229.0
36	210376.0
37	290246.0
38	454792.0
39	758255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80146816194344	17.018045390932777	13.042527960400088	25.137958486723694
2	16.27400117003825	18.935343007252964	38.88343405518704	25.907221767521747
3	20.00411737635402	22.977966730608625	27.911531589721132	29.106384303316226
4	13.552818777179814	15.356953610993399	35.60349712734957	35.48673048447722
5	15.272659361228689	36.40293306932738	33.15757696017521	15.166830609268716
6	34.441056102109904	34.56876419789098	16.582217390151417	14.407962309847703
7	30.27592185490309	30.662383568288575	20.096999026332853	18.964695550475486
8	26.98386601198691	34.647678266406864	18.949860567636627	19.418595153969605
9	26.741471331262563	16.054563679724776	18.332717523702776	38.871247465309885
10	15.524074092404808	28.47405365887459	32.36292919251497	23.63894305620564
11	36.19246640043442	22.428876677150033	22.010543702061224	19.36811322035432
12	24.159764215302033	24.035132938261025	28.768894013208442	23.036208833228503
13	28.800054587005462	20.970531502076003	24.866855788485253	25.36255812243328
14	22.731536166483234	20.55206861331262	25.70747137429207	31.008923845912072
15	24.741531426518844	28.08699491989887	22.455114695595206	24.71635895798708
16	24.829171349789405	27.079651211507205	24.065918097707588	24.0252593409958
17	23.7972369781769	27.031970089360684	25.314830691992896	23.85596224046952
18	23.684624558011862	26.726082789182488	25.868357980252533	23.720934672553124
19	24.57670268309293	26.386010097045638	25.86814579404092	23.169141425820516
20	24.811561875880486	25.700110043055407	25.804424636065832	23.683903444998275
21	24.94552972409625	25.643308015839082	25.68196148869271	23.729200771371957
22	24.846177949072327	25.246797834785323	25.517485035107292	24.38953918103506
23	24.464683671813372	25.556017948711602	25.532081147523883	24.44721723195114
24	24.240631421784677	25.594910772667014	25.628329249442228	24.536128556106085
25	24.53030971761291	25.390966498892347	25.730122421495484	24.348601361999258
26	24.308760771795836	25.729451319634506	25.76085561134218	24.200932297227475
27	24.41886165383382	25.72200911743498	25.589537193455335	24.269592035275867
28	24.336540321858983	25.779738213247228	25.570206945728074	24.31351451916571
29	24.295075056425823	25.95131712126796	25.570217349941966	24.183390472364255
30	24.572109197428677	25.8328738821544	25.54468394061639	24.050332979800533
31	24.64971238054158	25.462895852410732	25.367965521020793	24.51942624602689
32	24.385766802451915	25.847149322994706	25.51138665703136	24.255697217522023
33	24.503544231206416	25.652710243498177	25.305093093301195	24.538652431994212
34	24.630897847768807	25.63384882659084	25.529060265089605	24.20619306055075
35	24.651640844017194	25.710427810606323	25.46766980243826	24.17026154293822
36	24.633135067461428	25.711216794768127	25.376746994810063	24.278901142960382
37	24.663029127888763	25.452800742216308	25.58337452435857	24.300795605536358
38	24.578084095051057	25.62235181194838	25.540225943360372	24.259338149640193
39	24.632709355843946	25.796011474982095	25.38238744398804	24.18889172518592
40	24.683777920457985	25.546246772017454	25.57046548003247	24.19950982749209
41	24.39701153586185	25.769175294759794	25.516165478389	24.317647690989357
42	24.68060376414045	25.621479801177365	25.481917936350882	24.215998498331302
43	24.45073601074083	25.468576949276926	25.488125660387627	24.59256137959461
44	24.491843298864115	25.70164173295566	25.380032740629666	24.426482227550558
45	24.559600839549006	25.68100248202809	25.246771278035922	24.512625400386973
46	24.531121540450687	25.53214727283138	25.303177791020637	24.633553395697298
47	24.47353835669314	25.699097514524176	25.36407646878345	24.463287659999235
48	24.35737611165759	25.674275710522437	25.49274164160382	24.47560653621615
49	24.639513479276353	25.5283059148207	25.357376135997328	24.474804469905614
50	24.691006748225995	25.473386710250434	25.43093636760795	24.404670173915623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1901.0
1	1433.5
2	966.0
3	909.0
4	852.0
5	850.0
6	848.0
7	807.5
8	767.0
9	857.5
10	948.0
11	1092.5
12	1237.0
13	1790.0
14	2343.0
15	3031.5
16	3720.0
17	4156.5
18	4593.0
19	4799.0
20	5005.0
21	5715.5
22	6426.0
23	8175.0
24	9924.0
25	12831.5
26	15739.0
27	20264.0
28	24789.0
29	30673.0
30	36557.0
31	44593.5
32	52630.0
33	62210.5
34	71791.0
35	82798.0
36	93805.0
37	105364.5
38	116924.0
39	128324.0
40	139724.0
41	151350.0
42	162976.0
43	171997.0
44	181018.0
45	190860.0
46	200702.0
47	208429.0
48	216156.0
49	218182.5
50	220209.0
51	219847.5
52	219486.0
53	215504.5
54	211523.0
55	202925.5
56	194328.0
57	181737.5
58	169147.0
59	154453.0
60	139759.0
61	123634.5
62	107510.0
63	92125.0
64	76740.0
65	64453.0
66	52166.0
67	43152.0
68	34138.0
69	27728.5
70	21319.0
71	16968.0
72	12617.0
73	10502.0
74	8387.0
75	6454.5
76	4522.0
77	3490.0
78	2458.0
79	1967.5
80	1477.0
81	1145.5
82	814.0
83	638.5
84	463.0
85	354.5
86	246.0
87	197.0
88	148.0
89	116.5
90	85.0
91	64.5
92	44.0
93	30.5
94	17.0
95	15.5
96	14.0
97	18.5
98	23.0
99	24.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08922242567683603
2	0.09710226762770113
3	0.10469942466553074
4	0.10600684238832002
5	0.0988337127200437
6	0.05939916735158865
7	0.05385147593326657
8	0.061943331568908334
9	0.061872660340649456
10	0.04671368187911969
11	0.04939918855295713
12	0.05876312629725873
13	0.05279140750938337
14	0.0536041266343605
15	0.053674797862619375
16	0.056077619623421296
17	0.055865605938644654
18	0.05628963330819793
19	0.06544155736772289
20	0.07144861176972771
21	0.0818726179379125
22	0.08438144654110274
23	0.060953934373284016
24	0.07876308389452177
25	0.08473480268239714
26	0.0836393986443845
27	0.0790457688075573
28	0.09692558955705394
29	0.08480547391065603
30	0.0832860425030901
31	0.05710235243317506
32	0.053710133476748814
33	0.05678433190601009
34	0.05134264733007634
35	0.05918715366681201
36	0.058268427699446575
37	0.06077725630263682
38	0.05021190767793425
39	0.05625429769406849
40	0.05703168120491617
41	0.044593545031353286
42	0.04074196309124433
43	0.041519346602092014
44	0.03904585361303121
45	0.03067131306435393
46	0.02855117621658753
47	0.03286212114037921
48	0.027738457091610408
49	0.02734976533618657
50	0.028904532357881926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2830006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.80358398378094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85633563336056	48.11800577985835
2	12.342626779008627	15.256371414338469
3	4.262143140513543	7.902471646068738
4	1.9230460524436828	4.754045528275262
5	1.0474717384165597	3.2368753777932433
6	0.6208141945611017	2.302112532712822
7	0.4092889755146969	1.7706867900300762
8	0.28437698131230793	1.406041331807146
9	0.20163265206208023	1.121545849103213
>10	0.9888377532891117	10.489735886728388
>50	0.04866519289474312	2.0625281665180615
>100	0.014243751747731534	1.350056845642696
>500	4.0223156972798014E-4	0.15473171323098928
>1k	1.1492330552227977E-4	0.0747911378926055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0600684238832003E-4	0.0	0.0	0.0	0.0
3	1.0600684238832003E-4	0.0	0.0	0.0	0.0
4	1.0600684238832003E-4	0.0	0.0	0.0	0.0
5	1.4134245651776004E-4	1.7667807064720005E-4	0.0	0.0	0.0
6	2.1201368477664006E-4	1.7667807064720005E-4	0.0	0.0	0.0
7	2.4734929890608007E-4	1.7667807064720005E-4	0.0	0.0	0.0
8	2.826849130355201E-4	1.7667807064720005E-4	0.0	0.0	0.0
9	2.826849130355201E-4	1.7667807064720005E-4	0.0	0.0	0.0
10	2.826849130355201E-4	1.7667807064720005E-4	0.0	0.0	0.0
11	2.826849130355201E-4	1.7667807064720005E-4	0.0	0.0	0.0
12	3.533561412944001E-4	1.7667807064720005E-4	0.0	0.0	0.0
13	3.533561412944001E-4	2.1201368477664006E-4	0.0	0.0	0.0
14	3.886917554238401E-4	2.1201368477664006E-4	0.0	0.0	0.0
15	3.886917554238401E-4	2.1201368477664006E-4	0.0	0.0	0.0
16	4.240273695532801E-4	2.1201368477664006E-4	0.0	0.0	0.0
17	4.5936298368272013E-4	2.1201368477664006E-4	0.0	0.0	0.0
18	4.946985978121601E-4	2.4734929890608007E-4	0.0	0.0	0.0
19	6.713766684593601E-4	2.4734929890608007E-4	0.0	3.533561412944001E-5	0.0
20	6.713766684593601E-4	3.533561412944001E-4	0.0	3.533561412944001E-5	0.0
21	6.713766684593601E-4	3.533561412944001E-4	0.0	1.4134245651776004E-4	0.0
22	6.713766684593601E-4	3.533561412944001E-4	0.0	1.7667807064720005E-4	0.0
23	6.713766684593601E-4	3.886917554238401E-4	0.0	2.4734929890608007E-4	0.0
24	7.773835108476802E-4	3.886917554238401E-4	0.0	3.180205271649601E-4	0.0
25	7.773835108476802E-4	3.886917554238401E-4	0.0	3.180205271649601E-4	0.0
26	7.773835108476802E-4	3.886917554238401E-4	0.0	4.5936298368272013E-4	0.0
27	7.773835108476802E-4	3.886917554238401E-4	0.0	8.127191249771202E-4	0.0
28	9.187259673654403E-4	3.886917554238401E-4	0.0	0.0015901026358248003	0.0
29	0.0011660752662715202	3.886917554238401E-4	0.0	0.002862184744484641	0.0
30	0.0011660752662715202	4.946985978121601E-4	0.0	0.004734972293344961	0.0
31	0.0011660752662715202	4.946985978121601E-4	0.0	0.009363937744301602	0.0
32	0.0011660752662715202	5.300342119416001E-4	0.0	0.014876293548494244	0.0
33	0.0011660752662715202	5.300342119416001E-4	0.0	0.021625395847217285	0.0
34	0.0011660752662715202	5.300342119416001E-4	0.0	0.03197873078714321	0.0
35	0.0012014108804009602	6.007054402004801E-4	0.0	0.04378082590637617	0.0
36	0.0013074177227892802	6.007054402004801E-4	0.0	0.060070544020048015	0.0
37	0.0013074177227892802	6.713766684593601E-4	0.0	0.07985848793253442	0.0
38	0.0013780889510481603	6.713766684593601E-4	0.0	0.09996445237218578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	55	0.0044901534	19.996086	7
GTATCAA	3835	0.0	19.799074	1
GGTATCA	2150	0.0	19.34704	1
GTACCGA	290	0.0	18.961805	6
CGACGCA	60	0.0074168174	18.332336	31
CGAATCG	240	0.0	17.415415	37
TACCGAT	260	0.0	16.919765	7
GTATACG	105	3.7646845E-5	16.768408	1
CGTGCCG	230	1.8189894E-12	16.256824	10
ACCGTAT	110	5.6340872E-5	15.997151	8
GTACGAC	85	0.0029739132	15.535163	3
CAACGCA	4895	0.0	15.283314	5
TATCAAC	4890	0.0	15.16717	2
TCAACGC	4905	0.0	15.165119	4
ATCAACG	4905	0.0	15.075919	3
GCACCGT	445	0.0	14.828558	6
AACGCAG	5205	0.0	14.410243	6
GCGATAC	185	1.2680357E-7	14.269748	14
GTATAGA	495	0.0	14.227741	1
GGCGTAA	110	0.0010200994	13.99726	6
>>END_MODULE