FastQCFastQC Report
Mon 6 Feb 2017
SRR2077135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830006
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49050.020.6773761
GGTATCA27500.020.08051
ATACCGT1601.1023076E-917.8763926
ATTCGGT2201.8189894E-1116.0015326
TATCAAC65650.015.2481752
TGCGATA1809.038922E-814.67014313
GCGATAC1951.5139449E-814.66988414
TATACTG6600.014.6683285
ATCAACG67850.014.5918883
TCAACGC67900.014.5168524
CAACGCA68800.014.3272055
TGTACGG2002.1178494E-814.3006092
TATAACG1251.6716658E-414.08062
CGTGCCG3150.013.97131810
CTATACT5050.013.9419274
AACGCAG71100.013.8013576
GTACATG74400.013.661631
ATAACGC1453.83719E-513.6559943
CGTACAC2451.5352271E-913.47023
GTACCGA2959.094947E-1213.4247756
Produced by FastQC (version 0.11.5)