##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077135_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2830006
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.433813214530286	31.0	31.0	34.0	27.0	34.0
2	30.566252863068136	31.0	31.0	34.0	27.0	34.0
3	30.631469685929993	31.0	31.0	34.0	27.0	34.0
4	34.15871485784836	37.0	35.0	37.0	30.0	37.0
5	33.70823065392794	37.0	35.0	37.0	28.0	37.0
6	33.6321046669159	37.0	35.0	37.0	28.0	37.0
7	33.45998877740895	37.0	35.0	37.0	28.0	37.0
8	33.3847380535589	37.0	35.0	37.0	27.0	37.0
9	34.80977390153943	39.0	35.0	39.0	27.0	39.0
10	34.668222964898305	39.0	35.0	39.0	27.0	39.0
11	34.678533190389	39.0	35.0	39.0	27.0	39.0
12	34.60247646118065	39.0	35.0	39.0	27.0	39.0
13	34.56783519186885	39.0	35.0	39.0	27.0	39.0
14	35.619225542278	40.0	36.0	41.0	27.0	41.0
15	35.6160251957063	40.0	36.0	41.0	27.0	41.0
16	35.584363425377894	40.0	36.0	41.0	27.0	41.0
17	35.567552860311956	40.0	36.0	41.0	27.0	41.0
18	35.486769992713796	40.0	36.0	41.0	26.0	41.0
19	35.54276068672646	40.0	36.0	41.0	26.0	41.0
20	35.51926709696022	40.0	36.0	41.0	26.0	41.0
21	35.48250145052696	40.0	36.0	41.0	26.0	41.0
22	35.39348538483664	39.0	35.0	41.0	25.0	41.0
23	35.34984272118151	39.0	35.0	41.0	25.0	41.0
24	35.33653073527053	39.0	35.0	41.0	25.0	41.0
25	35.26472417372967	39.0	35.0	41.0	25.0	41.0
26	35.20528437042183	39.0	35.0	41.0	25.0	41.0
27	35.09527859658248	39.0	35.0	41.0	24.0	41.0
28	35.058956058750404	39.0	35.0	41.0	24.0	41.0
29	35.02558793161569	39.0	35.0	41.0	24.0	41.0
30	34.95214709792135	39.0	35.0	41.0	24.0	41.0
31	34.87041546908381	39.0	35.0	41.0	24.0	41.0
32	34.798246010785846	39.0	34.0	41.0	24.0	41.0
33	34.68269042539132	39.0	34.0	41.0	23.0	41.0
34	34.633847419404766	39.0	34.0	41.0	23.0	41.0
35	34.56552318263636	39.0	34.0	41.0	22.0	41.0
36	34.49444912837641	39.0	34.0	41.0	22.0	41.0
37	34.40885178335311	39.0	34.0	41.0	21.0	41.0
38	34.243876161393295	39.0	34.0	41.0	18.0	41.0
39	34.09473478148103	39.0	33.0	41.0	17.0	41.0
40	34.07471079566616	39.0	33.0	41.0	18.0	41.0
41	33.96399018235297	38.0	33.0	41.0	16.0	41.0
42	34.09156659031818	39.0	34.0	41.0	17.0	41.0
43	34.07636379569513	39.0	34.0	41.0	16.0	41.0
44	33.98736751794873	39.0	34.0	41.0	15.0	41.0
45	33.8453031548343	39.0	33.0	41.0	11.0	41.0
46	33.73677087610415	39.0	33.0	41.0	9.0	41.0
47	33.59274927332309	38.0	33.0	41.0	2.0	41.0
48	33.44811671777374	38.0	33.0	41.0	2.0	41.0
49	33.189118680313754	38.0	33.0	40.0	2.0	41.0
50	33.06589031966717	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.9667280214494944
1101	2	1.2014639921742294
1101	3	1.243906017310227
1101	4	1.2744231719291221
1101	5	1.446873239316865
1101	6	1.5513956274844034
1101	7	1.6658266280285474
1101	8	1.7176070238845398
1101	9	1.8635620414550687
1101	10	2.003562406065548
1101	11	2.0334869642311872
1101	12	1.9859518829453506
1101	13	2.0686092221060832
1101	14	2.235922096416772
1101	15	2.2839597316770863
1101	16	2.292419621056254
1101	17	2.3289970483970137
1101	18	2.383141090450515
1101	19	2.262203696564221
1101	20	2.290323448862118
1101	21	2.3352006979408344
1101	22	2.438370298372959
1101	23	2.518358135043975
1101	24	2.4589270672192356
1101	25	2.509015012161889
1101	26	2.440876572673268
1101	27	2.495955929440292
1101	28	2.4913302661515004
1101	29	2.507476604507353
1101	30	2.449970939376165
1101	31	2.500775004148359
1101	32	2.5711000341082055
1101	33	2.6713981722317612
1101	34	2.6353407702053246
1101	35	2.6109401595824053
1101	36	2.7254012463338597
1101	37	2.694349378752676
1101	38	2.7437126281826494
1101	39	2.8093016728059084
1101	40	2.794747315597803
1101	41	2.7013584272824502
1101	42	2.7460358572154107
1101	43	2.8371540251147565
1101	44	2.864926283490256
1101	45	3.002696855170278
1101	46	3.015378892082637
1101	47	2.763515446247851
1101	48	2.9238814797833967
1101	49	3.0397474675943243
1101	50	3.094750945978916
1102	1	0.45021037871826053
1102	2	0.6224529277262718
1102	3	0.616170574040801
1102	4	0.6359534072457649
1102	5	0.8815949361744799
1102	6	0.917990916409785
1102	7	0.996940677589194
1102	8	0.9490127989796306
1102	9	1.0127181711937254
1102	10	1.078218422469142
1102	11	1.0491422905661238
1102	12	1.1053459732132325
1102	13	1.096005773774479
1102	14	1.2417257138708777
1102	15	1.3312741576322864
1102	16	1.3502510381109687
1102	17	1.16054234596173
1102	18	1.1902940238170387
1102	19	1.353989652427849
1102	20	1.3009866439913367
1102	21	1.3375640304012535
1102	22	1.4313420874612532
1102	23	1.4301134060225422
1102	24	1.2703102184082127
1102	25	1.390189932171765
1102	26	1.3506557542971152
1102	27	1.3603317549811678
1102	28	1.441751653466092
1102	29	1.4157535127842635
1102	30	1.4053499724250997
1102	31	1.41780691147153
1102	32	1.4305394081106826
1102	33	1.3993742594311485
1102	34	1.3277454529012687
1102	35	1.4526001177472239
1102	36	1.5286986321555176
1102	37	1.4638538764125073
1102	38	1.4995199276792661
1102	39	1.502894308146118
1102	40	1.5105626042286247
1102	41	1.5943464173578192
1102	42	1.5942290160881853
1102	43	1.556099947944169
1102	44	1.558005228996386
1102	45	1.5938216598873183
1102	46	1.6166808623166418
1102	47	1.6594982839782588
1102	48	1.7067900490997374
1102	49	1.7817756659331963
1102	50	1.8116891677715046
1103	1	0.3256713093008159
1103	2	0.40666831560155714
1103	3	0.3824678373253434
1103	4	0.2759951247255543
1103	5	0.41946414376027263
1103	6	0.4207384519624995
1103	7	0.5054136736765642
1103	8	0.509360524022739
1103	9	0.637430090482745
1103	10	0.6043028003723094
1103	11	0.6432135717608176
1103	12	0.5823975734516154
1103	13	0.6693844890858003
1103	14	0.7424007271546813
1103	15	0.7031607285815724
1103	16	0.7182704652689793
1103	17	0.6366964909397979
1103	18	0.7688870195044331
1103	19	0.6601516564251568
1103	20	0.6519115916580773
1103	21	0.6783439824287001
1103	22	0.81642102531859
1103	23	0.8319572497320422
1103	24	0.8352753267788131
1103	25	0.9191752170151162
1103	26	0.909026731101477
1103	27	0.8784334223798709
1103	28	0.805428534661381
1103	29	0.7366771443528393
1103	30	0.7835483814202107
1103	31	0.6696613989876994
1103	32	0.6935209652230157
1103	33	0.7203667303901469
1103	34	0.6455040909009213
1103	35	0.6654927818784486
1103	36	0.6793154614155057
1103	37	0.6200313793876049
1103	38	0.6920744534183498
1103	39	0.8349684759712304
1103	40	0.7212694587649864
1103	41	0.6978006924255737
1103	42	0.6554404702922767
1103	43	0.686008432014674
1103	44	0.6836439946708595
1103	45	0.7252786545444891
1103	46	0.73726932523941
1103	47	0.7501709742938871
1103	48	0.7259487277094294
1103	49	0.7927809516539526
1103	50	0.8406336749574734
1104	1	0.3840812884304121
1104	2	0.46268943362282755
1104	3	0.3668117616966562
1104	4	0.3904904370200626
1104	5	0.4243019894509956
1104	6	0.460595453406043
1104	7	0.4292819997061841
1104	8	0.5190942689748184
1104	9	0.6171007505547337
1104	10	0.6640100794428463
1104	11	0.5851198064832417
1104	12	0.6096245055331764
1104	13	0.6499612373503041
1104	14	0.7848999843963114
1104	15	0.7043392256930403
1104	16	0.7094764083372027
1104	17	0.7687300950036615
1104	18	0.8266387212057396
1104	19	0.7072877380382678
1104	20	0.71537800790815
1104	21	0.7783090058744477
1104	22	0.7886918287626798
1104	23	0.8670370103318277
1104	24	0.7099695827871599
1104	25	0.7773186696024865
1104	26	0.7826715942076916
1104	27	0.8429713072928493
1104	28	0.8679369707212956
1104	29	0.8683685885658576
1104	30	0.7005147348029794
1104	31	0.825313371393861
1104	32	0.7904995993909765
1104	33	0.8152411873774597
1104	34	0.7296211153463901
1104	35	0.8330930070839386
1104	36	0.7402802790262086
1104	37	0.7691902091431473
1104	38	0.7901359840416546
1104	39	0.9766929346626014
1104	40	0.9137057813148601
1104	41	0.8603566111074414
1104	42	0.7993326955231836
1104	43	0.847601219816454
1104	44	0.8585302799528449
1104	45	0.835479013494627
1104	46	0.8125277406484201
1104	47	0.8774944890322729
1104	48	0.8261952580217695
1104	49	0.8835106436388429
1104	50	0.80691682542367
1105	1	0.4297664315893357
1105	2	0.5437182717678688
1105	3	0.4360115151578903
1105	4	0.41644299927137496
1105	5	0.4876128589048534
1105	6	0.47347976558128835
1105	7	0.48660066819995507
1105	8	0.4904686825018558
1105	9	0.7086660949041672
1105	10	0.6860982139534073
1105	11	0.8153143066853232
1105	12	0.6711273055274134
1105	13	0.7392949446360149
1105	14	0.7465973319899035
1105	15	0.8022424065426463
1105	16	0.8094288378761334
1105	17	0.7113353174181256
1105	18	0.8275508909492544
1105	19	0.7651464620634201
1105	20	0.791826992849856
1105	21	0.7361417890043711
1105	22	0.8313304206843242
1105	23	0.8082386808306836
1105	24	0.8217747956492545
1105	25	0.8091583384632202
1105	26	0.8337213345526351
1105	27	0.8038433497710216
1105	28	0.7697038774673359
1105	29	0.8489191608241953
1105	30	0.8024031153054167
1105	31	0.7743577875660321
1105	32	0.8670935106271784
1105	33	0.8808203356374236
1105	34	0.7210431193871898
1105	35	0.7504714836515447
1105	36	0.792007390287857
1105	37	0.7933199358595573
1105	38	0.857956476692614
1105	39	1.0572495318442492
1105	40	0.8688209229771218
1105	41	0.9399352292470766
1105	42	0.9113107261553139
1105	43	0.9188953405303906
1105	44	0.9292411406928949
1105	45	0.9118383537755506
1105	46	0.9618125795168169
1105	47	0.9058623072279843
1105	48	1.0459997278403677
1105	49	1.0909277435196714
1105	50	1.1097006225545627
1106	1	0.15474925084539137
1106	2	0.20405552570488084
1106	3	0.18836714411061806
1106	4	0.07220519207262299
1106	5	0.16218260787231742
1106	6	0.15550434426774729
1106	7	0.18894139234618734
1106	8	0.16294618666886862
1106	9	0.12743633737935056
1106	10	0.08273263944613518
1106	11	0.07333209952837194
1106	12	0.15783632515062607
1106	13	0.0903745837536647
1106	14	0.19362514641684214
1106	15	0.229162616983956
1106	16	0.2355410792921191
1106	17	0.1966377942222124
1106	18	0.1572129926500878
1106	19	0.04072074196625408
1106	20	0.03676347015522907
1106	21	0.11259950109814554
1106	22	0.16318339863051534
1106	23	0.15969311943892706
1106	24	0.19428967641574246
1106	25	0.20003857400679692
1106	26	0.24304120176550725
1106	27	0.2813355192431217
1106	28	0.12570870811646984
1106	29	0.2774123967740678
1106	30	0.19771857551254612
1106	31	0.17251284482484408
1106	32	0.1709348886167561
1106	33	0.24445420744601876
1106	34	0.27067059808076976
1106	35	0.24022805765078914
1106	36	0.19791272225433687
1106	37	0.24369677088747466
1106	38	0.2417547947546268
1106	39	0.33030010492664275
1106	40	0.2597922409529474
1106	41	0.24286681640990793
1106	42	0.2661629394044951
1106	43	0.20864239738155987
1106	44	0.2729372163656336
1106	45	0.25067407854322
1106	46	0.32943938473063383
1106	47	0.40870621800117135
1106	48	0.48253162875454336
1106	49	0.6214079604782938
1106	50	0.5723555222277383
1107	1	0.05248016280622636
1107	2	-0.003597789233538151
1107	3	0.06360529420279448
1107	4	-0.04726573051883065
1107	5	-0.10138168316577634
1107	6	-0.1181278469817002
1107	7	-0.061781744769589864
1107	8	-0.04839719843036505
1107	9	-0.0658295437176264
1107	10	-0.018741856309318905
1107	11	-0.014147954542302443
1107	12	-0.12464625677900187
1107	13	-0.2106269874037352
1107	14	-0.11644809884839447
1107	15	-0.061511507880652516
1107	16	-0.06436128559226262
1107	17	-0.005359092666985532
1107	18	-0.01024281308342978
1107	19	-0.08136623747349603
1107	20	-0.12652860170572922
1107	21	-0.11973624508083702
1107	22	0.0941472957210081
1107	23	0.15140558186017472
1107	24	0.059837189753366715
1107	25	0.07451570740663271
1107	26	0.07924702834293385
1107	27	-0.08265286982163644
1107	28	-0.11658226850419595
1107	29	-0.006464000342340626
1107	30	0.05534644060338678
1107	31	0.025256700739497262
1107	32	0.006367620302533794
1107	33	-0.022984526564002294
1107	34	-0.02275253396035737
1107	35	-0.05343929738310038
1107	36	-0.08313061603736571
1107	37	0.015383155214514943
1107	38	0.05493421036627666
1107	39	0.11809055970600468
1107	40	0.05936060757208139
1107	41	-0.12242502668635069
1107	42	-0.006439675489595231
1107	43	-0.13109837561778903
1107	44	-0.11822090177520295
1107	45	-0.04369570490277397
1107	46	0.041300014208523805
1107	47	-0.012489660736953567
1107	48	-0.01652703785371301
1107	49	0.1577027691330315
1107	50	0.07320879292749538
1108	1	0.07873342525516236
1108	2	0.062352007348213334
1108	3	0.05821163963845066
1108	4	0.10996216300971184
1108	5	0.12196631742346398
1108	6	0.07274028132081867
1108	7	0.0021595627157040553
1108	8	-0.026443786176720607
1108	9	0.012576408927252203
1108	10	0.012036379381783036
1108	11	0.007284738315959771
1108	12	-0.02363795022797177
1108	13	0.05686862147335603
1108	14	0.06352456338335344
1108	15	0.04812047955944365
1108	16	-0.03816919788432216
1108	17	0.09165425859789167
1108	18	0.1574518339309705
1108	19	0.12366604566081918
1108	20	0.0027625005016034265
1108	21	-0.06730361927009199
1108	22	0.06898098607013026
1108	23	0.13843066399338255
1108	24	0.06903052983059865
1108	25	-0.002254677362913071
1108	26	0.05595413396039106
1108	27	0.10256369120848774
1108	28	0.037175304316996005
1108	29	-0.037458060519689695
1108	30	0.0171959145250824
1108	31	0.14357648019721836
1108	32	0.10918229460576612
1108	33	0.21061929700412918
1108	34	0.17875510529660232
1108	35	0.10666429837029057
1108	36	0.03152161534801934
1108	37	0.04517965866207874
1108	38	0.189378183357384
1108	39	0.1784626881024849
1108	40	0.17276046965974956
1108	41	0.11578990415927137
1108	42	0.0390802045883305
1108	43	-0.027212898648123485
1108	44	-0.07224049824703371
1108	45	-0.07354065874520899
1108	46	-0.025965773401587455
1108	47	-0.039293985252804475
1108	48	-0.1221728791703498
1108	49	-0.0358731979050404
1108	50	-0.06248754936132883
1109	1	-0.004099621046286472
1109	2	-0.1031951615581832
1109	3	-0.060361647945967434
1109	4	-0.001281411807241284
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2211	9	-0.07070491912591592
2211	10	-0.0996415209080439
2211	11	-0.10837452212309984
2211	12	-0.05836541118780758
2211	13	-0.1195878280260203
2211	14	0.041402410099294684
2211	15	-0.007955450562839417
2211	16	-0.09808555505320982
2211	17	-0.12403999379302633
2211	18	-0.1944446091974541
2211	19	-0.133643531757059
2211	20	-0.06493606675896046
2211	21	-0.0910831844528559
2211	22	-0.014588482607244657
2211	23	0.019180022675371333
2211	24	-0.0765929422880518
2211	25	-0.09705921714635224
2211	26	-0.070787680939425
2211	27	-0.06984624071407097
2211	28	-0.03444622273178766
2211	29	-0.07708825591957691
2211	30	-0.033382176651876705
2211	31	-0.03611515624364614
2211	32	-0.0073085676683248835
2211	33	0.16165131825993484
2211	34	0.1646395932436846
2211	35	0.1705649115740826
2211	36	0.15639558399040254
2211	37	0.11943354578528442
2211	38	0.07939292824192279
2211	39	0.136132079314919
2211	40	0.0998382072973314
2211	41	0.08706851007990934
2211	42	0.03519392601861426
2211	43	0.005413597257820868
2211	44	-0.06743574127217045
2211	45	-0.09860213598475553
2211	46	-0.09466245592322764
2211	47	-0.018655490727233826
2211	48	-0.010735337391167832
2211	49	0.22635407602884783
2211	50	0.1709135959614514
2212	1	-0.22081550923329019
2212	2	-0.28903881912876983
2212	3	-0.20706816708958087
2212	4	-0.27951303090021895
2212	5	-0.28628928189873193
2212	6	-0.264774068539154
2212	7	-0.2486744023391978
2212	8	-0.36822912347018644
2212	9	-0.3824495431676098
2212	10	-0.33766028404375703
2212	11	-0.3980603271530825
2212	12	-0.34815619217469873
2212	13	-0.4163154483325471
2212	14	-0.4134250137283999
2212	15	-0.44836377719254017
2212	16	-0.37634166391222124
2212	17	-0.5241097729617081
2212	18	-0.5278234087061051
2212	19	-0.4200681919785936
2212	20	-0.4133256820387601
2212	21	-0.42734346865156425
2212	22	-0.39058642346347483
2212	23	-0.43956736664016915
2212	24	-0.36891754093714724
2212	25	-0.2858860801075238
2212	26	-0.47841397355463755
2212	27	-0.36818979735390656
2212	28	-0.427719993620002
2212	29	-0.36209600404047393
2212	30	-0.46536473449287996
2212	31	-0.53091261452645
2212	32	-0.4216927313957086
2212	33	-0.39564514709602605
2212	34	-0.5111045533153913
2212	35	-0.605066491158361
2212	36	-0.5571779564510138
2212	37	-0.6374050541198315
2212	38	-0.40774220679832496
2212	39	-0.29038584800678535
2212	40	-0.46850052624147764
2212	41	-0.505554910178958
2212	42	-0.5602039403187291
2212	43	-0.5563748033378033
2212	44	-0.5390610736558088
2212	45	-0.38310387541705637
2212	46	-0.42814864513361073
2212	47	-0.6053066918724923
2212	48	-0.6021395531940001
2212	49	-0.39995587426117396
2212	50	-0.5280274722391312
2213	1	-0.40379217694175296
2213	2	-0.3423266144013368
2213	3	-0.5065905404330202
2213	4	-0.4429085942681752
2213	5	-0.523263250451663
2213	6	-0.3955703943890896
2213	7	-0.3792400273207903
2213	8	-0.40896848717844847
2213	9	-0.44226300478192826
2213	10	-0.3741863448527525
2213	11	-0.3733851512983364
2213	12	-0.48098696947330666
2213	13	-0.5243563686871155
2213	14	-0.4726699784253725
2213	15	-0.47051695078865663
2213	16	-0.4443538863282086
2213	17	-0.5932786233288567
2213	18	-0.6224037142950607
2213	19	-0.728428952090205
2213	20	-0.6994218895663735
2213	21	-0.7030997257321161
2213	22	-0.5638749933911811
2213	23	-0.5575568938024489
2213	24	-0.39888205851887193
2213	25	-0.43918756571402184
2213	26	-0.5789104846613924
2213	27	-0.4765910929914057
2213	28	-0.5487554862123218
2213	29	-0.5922339979656783
2213	30	-0.41424633059661886
2213	31	-0.49800297947648176
2213	32	-0.5622014299340208
2213	33	-0.6227387820189065
2213	34	-0.47346898643471746
2213	35	-0.5682050439419157
2213	36	-0.5679428070273573
2213	37	-0.5709909595372977
2213	38	-0.47865751182413874
2213	39	-0.4742006247499191
2213	40	-0.6130527818177001
2213	41	-0.5285170093730116
2213	42	-0.5185255303680165
2213	43	-0.7113464382867463
2213	44	-0.6680705164163783
2213	45	-0.6234731181809465
2213	46	-0.634360504746823
2213	47	-0.5221016793896567
2213	48	-0.7184912380600039
2213	49	-0.6164973317873148
2213	50	-0.6518125443489922
2214	1	-0.25241890083409757
2214	2	-0.41186958736547297
2214	3	-0.377150919280691
2214	4	-0.2822836263758859
2214	5	-0.41089676016336796
2214	6	-0.36693498390072676
2214	7	-0.4316129625029035
2214	8	-0.4148704687497897
2214	9	-0.4630197660826738
2214	10	-0.4699840872718326
2214	11	-0.46880343891806575
2214	12	-0.4340419892516536
2214	13	-0.49118267879858735
2214	14	-0.4735106167500689
2214	15	-0.6181937533740864
2214	16	-0.6551801403971282
2214	17	-0.6808135915480591
2214	18	-0.6571512659359371
2214	19	-0.682998288406246
2214	20	-0.7535286412931796
2214	21	-0.7984537979242674
2214	22	-0.731369844481442
2214	23	-0.6872720575167648
2214	24	-0.6209039804982979
2214	25	-0.5430904626470934
2214	26	-0.6833714720378055
2214	27	-0.6484023883683605
2214	28	-0.7093956322965767
2214	29	-0.7806327220635154
2214	30	-0.687277485586975
2214	31	-0.686818520076983
2214	32	-0.6266235144368508
2214	33	-0.6241164948288329
2214	34	-0.6396013029070247
2214	35	-0.6346972003193301
2214	36	-0.7066457954965983
2214	37	-0.7100021820589362
2214	38	-0.6403145967706436
2214	39	-0.6016215463697421
2214	40	-0.7045072262258216
2214	41	-0.7065186719688086
2214	42	-0.6491116887909811
2214	43	-0.6104628340032292
2214	44	-0.6612064709845455
2214	45	-0.48760261384465053
2214	46	-0.584300492510863
2214	47	-0.5234291874107484
2214	48	-0.5749709249689303
2214	49	-0.5045149665112802
2214	50	-0.4221524891470736
2215	1	-0.6060186562341805
2215	2	-0.5917359566753788
2215	3	-0.6463482872622528
2215	4	-0.7260988712198895
2215	5	-0.6484997580539513
2215	6	-0.7518805270178888
2215	7	-0.8389174303053863
2215	8	-0.7844418022561257
2215	9	-1.1007177006008106
2215	10	-1.067016000880784
2215	11	-0.9711678619628756
2215	12	-1.0685513891339795
2215	13	-1.2254802376811398
2215	14	-1.1459240111648654
2215	15	-1.2036187328837045
2215	16	-1.0575425079804504
2215	17	-1.060565882745287
2215	18	-1.1684428151629618
2215	19	-1.2265734296549908
2215	20	-1.1916952210309546
2215	21	-1.1665291408651655
2215	22	-1.1705371770319886
2215	23	-1.1472792002454426
2215	24	-1.1827862179709001
2215	25	-1.3000141562086185
2215	26	-1.2279724170362272
2215	27	-1.195810965295287
2215	28	-1.3763138788851563
2215	29	-1.4559044926123477
2215	30	-1.353352992964794
2215	31	-1.2656679498148833
2215	32	-1.2436659879792131
2215	33	-1.14455035136276
2215	34	-1.354144592210659
2215	35	-1.4465569561818867
2215	36	-1.2784687585984997
2215	37	-1.3311857681712098
2215	38	-1.3291858144003328
2215	39	-1.2690202570815217
2215	40	-1.3920513865312572
2215	41	-1.3078985289600737
2215	42	-1.3773771105331392
2215	43	-1.2322566352452355
2215	44	-1.2263729505185736
2215	45	-1.3112709959652165
2215	46	-1.2471097431153026
2215	47	-1.2994641765039532
2215	48	-1.4696336622783832
2215	49	-1.2978331750738192
2215	50	-1.468365686751433
2216	1	-1.129975004009495
2216	2	-1.0662272245132023
2216	3	-1.0638119983146517
2216	4	-1.036283081939878
2216	5	-1.247073100120673
2216	6	-1.3087844425689639
2216	7	-1.379888676358803
2216	8	-1.4421252218194027
2216	9	-1.4817493198839387
2216	10	-1.5270851565632526
2216	11	-1.6008129331179148
2216	12	-1.6268093479251462
2216	13	-1.6869186464380803
2216	14	-1.9006118293141228
2216	15	-1.9379166641009036
2216	16	-2.0148407752859256
2216	17	-1.9476545732521444
2216	18	-1.9194698469637785
2216	19	-2.07434629285725
2216	20	-1.9754252366578342
2216	21	-2.068840299731889
2216	22	-2.1794893911668325
2216	23	-1.8547214703377577
2216	24	-2.086945739695203
2216	25	-2.2164639600753517
2216	26	-2.138767596990647
2216	27	-2.191640158855151
2216	28	-2.169551512921821
2216	29	-2.2700455908114563
2216	30	-2.327581032539804
2216	31	-2.122405293147459
2216	32	-2.216486259253969
2216	33	-2.1623351861765983
2216	34	-2.130454539197032
2216	35	-2.246852689332705
2216	36	-2.3398745398074823
2216	37	-2.540372876119328
2216	38	-2.495922765221053
2216	39	-2.5862772861102243
2216	40	-2.7079137718748676
2216	41	-2.779469753915979
2216	42	-2.7333810699964793
2216	43	-2.490991127960463
2216	44	-2.4907394977144435
2216	45	-2.5301130369287392
2216	46	-2.6876277606360475
2216	47	-2.6797229154124302
2216	48	-2.631605641838327
2216	49	-2.4532644352085953
2216	50	-2.657984974783993
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76222.0
3	11429.0
4	19384.0
5	11673.0
6	8794.0
7	6919.0
8	5970.0
9	5332.0
10	5078.0
11	5014.0
12	5133.0
13	5359.0
14	5772.0
15	6230.0
16	6887.0
17	7306.0
18	7932.0
19	8906.0
20	10039.0
21	12587.0
22	15017.0
23	18586.0
24	21568.0
25	24494.0
26	27835.0
27	32011.0
28	37175.0
29	42702.0
30	50448.0
31	60854.0
32	74298.0
33	92680.0
34	117878.0
35	152183.0
36	198085.0
37	269752.0
38	421402.0
39	941072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17362913994806	17.858928598561747	14.469654485916216	26.49778777557398
2	15.666357802840276	19.16818094755464	37.78150252003197	27.38395872957311
3	18.845492389937395	22.77767903426251	28.532125230962652	29.84470334483744
4	13.203332027030227	15.79656160762212	34.9482255185664	36.051880846781245
5	14.825134579843638	36.42641908028976	33.54201987836556	15.206426461501044
6	34.49515271390055	34.97277687214673	16.279904086367512	14.252166327585208
7	29.90277735069606	30.662122752828548	20.22292605289192	19.21217384358347
8	27.12765637818844	34.62332547567466	18.762943663450436	19.486074482686462
9	26.5300575131323	15.500656924321682	18.44007955906716	39.529206003478855
10	15.261564043078682	28.532115831843686	32.59738639211612	23.60893373296151
11	36.51257172772574	21.969036316596362	22.00081515005513	19.51757680562277
12	24.021344010994373	23.87263863497867	29.100514512824642	23.00550284120231
13	28.943196754867945	20.588728157968976	25.195025394134074	25.273049693029005
14	22.642164446091062	20.155011355055475	26.06978621951599	31.133037979337473
15	24.663073443307745	27.92515037166034	22.73367794904863	24.67809823598328
16	24.65702960271859	26.94161336442611	24.372711704054836	24.02864532880046
17	23.537992519686295	26.85414236778551	25.731317511705004	23.876547600823194
18	23.493686047790106	26.377256125606625	26.421730785479763	23.707327041123506
19	24.334208787514225	26.013645489490127	26.591098791038903	23.061046931956742
20	24.58076839183692	25.3343689139166	26.431697815983174	23.653164878263308
21	24.654565713998863	25.222730258297105	26.452457226811198	23.67024680089283
22	24.446094157364936	24.989120732472536	26.294526782794676	24.270258327367852
23	24.25471787825129	25.117710545358907	26.27705203797705	24.350519538412755
24	24.02657175944583	25.262012932381435	26.255838024255638	24.4555772839171
25	24.140420137708112	25.144668459129793	26.46982093986246	24.245090463299633
26	24.07808497182794	25.389245941926575	26.44316611562155	24.089502970623926
27	24.160285455929877	25.429092382209483	26.332241970137982	24.078380191722655
28	24.075654313306845	25.426487846952195	26.33648176689337	24.161376072847588
29	24.107413021285502	25.490956419443418	26.286829684888623	24.11480087438246
30	24.224641858929516	25.49060916944358	26.332395776149948	23.95235319547696
31	24.30865407625015	25.12354709989067	26.16254100710372	24.405257816755466
32	24.047723400795977	25.43156771124197	26.23101066725104	24.28969822071101
33	24.206390721267628	25.308056403020903	26.05451707670166	24.43103579900981
34	24.356075774128094	25.30963179943232	26.261703415801673	24.07258901063791
35	24.362386267890365	25.37795251101272	26.168917275971072	24.090743945125848
36	24.239192015152014	25.411889693400752	26.085127934350698	24.263790357096536
37	24.331931536125406	25.129856796148243	26.28908736392736	24.24912430379899
38	24.36101640826248	25.32019418923661	26.119597358082082	24.199192044418826
39	24.40162845268723	25.51101184586243	25.975622685250627	24.11173701619971
40	24.425920612012096	25.19241996107074	26.280113988948838	24.101545437968326
41	24.14011345593944	25.403550259892764	26.05518158215661	24.401154702011187
42	24.34166470247121	25.385191391558564	26.111737572650203	24.161406333320024
43	24.20921324173805	25.160574449438837	26.0428151414921	24.587397167331016
44	24.289309625160875	25.373111121633833	25.92911153720298	24.40846771600231
45	24.373272045728243	25.38067998710925	25.72340858591855	24.52263938124396
46	24.326949749374098	25.228390252004036	25.85226058026478	24.59239941835709
47	24.25651802268653	25.399961342223442	25.836221771254092	24.50729886383593
48	24.214926233861505	25.497737244770857	25.83290134797101	24.454435173396625
49	24.477138548214068	25.333957125148938	25.701836463835388	24.487067862801606
50	24.495604015450347	25.264375687507528	25.835336005447406	24.404684291594712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	522.0
1	370.0
2	218.0
3	328.5
4	439.0
5	496.5
6	554.0
7	511.5
8	469.0
9	588.5
10	708.0
11	1098.0
12	1488.0
13	2563.5
14	3639.0
15	5090.0
16	6541.0
17	7372.5
18	8204.0
19	8507.5
20	8811.0
21	9129.5
22	9448.0
23	10801.0
24	12154.0
25	14969.5
26	17785.0
27	22195.5
28	26606.0
29	32765.5
30	38925.0
31	46792.0
32	54659.0
33	64043.5
34	73428.0
35	83544.0
36	93660.0
37	104555.5
38	115451.0
39	126468.0
40	137485.0
41	148984.0
42	160483.0
43	169466.5
44	178450.0
45	188695.0
46	198940.0
47	206539.0
48	214138.0
49	216499.5
50	218861.0
51	219248.5
52	219636.0
53	216156.0
54	212676.0
55	203201.0
56	193726.0
57	181314.5
58	168903.0
59	153727.5
60	138552.0
61	122480.0
62	106408.0
63	90830.5
64	75253.0
65	63156.5
66	51060.0
67	42037.0
68	33014.0
69	26589.5
70	20165.0
71	15976.5
72	11788.0
73	9810.0
74	7832.0
75	5945.0
76	4058.0
77	3119.5
78	2181.0
79	1740.5
80	1300.0
81	983.5
82	667.0
83	513.0
84	359.0
85	286.0
86	213.0
87	152.0
88	91.0
89	61.0
90	31.0
91	22.0
92	13.0
93	11.5
94	10.0
95	6.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0017314450923425603
2	0.009893971956243202
3	1.0600684238832003E-4
4	0.004840979135733281
5	0.042473408183586886
6	0.028056477618775368
7	0.025688991472102886
8	0.032473429384955364
9	0.03226141570017873
10	0.03685504553700593
11	0.03812712764566577
12	0.03257943622734369
13	0.04946985978121601
14	0.04628965450956641
15	0.04763240784648513
16	0.05236738013983009
17	0.05353345540610161
18	0.05049459259096977
19	0.04872781188449777
20	0.04660767503673137
21	0.04409884643354113
22	0.04349814099334065
23	0.043992839591152806
24	0.04021192887930273
25	0.039717230281490566
26	0.04024726449343217
27	0.03985857273800833
28	0.03837447694457185
29	0.036678367466358726
30	0.03614833325441713
31	0.03261477184147313
32	0.027773792705739847
33	0.023109491640653766
34	0.021095361635275686
35	0.019434587771192006
36	0.019328580928803685
37	0.019187238472285924
38	0.013957567581128802
39	0.011519410206197442
40	0.009116588445395523
41	0.0065370886139464015
42	0.006325074929169761
43	0.005865711945487041
44	0.005371013347674881
45	0.0024734929890608006
46	0.003215540885779041
47	0.0015901026358248005
48	0.0017314450923425603
49	7.067122825888002E-5
50	0.0013074177227892802
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2830006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.75998800900296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25640661602965	45.57081944983585
2	13.187942858031604	15.762226141187696
3	4.603672973918362	8.25346325156197
4	2.073802958004804	4.957217596136078
5	1.0964916653171315	3.2763164385661727
6	0.6759541089511065	2.4237005667332645
7	0.4288791253749193	1.7940867972801797
8	0.30498433565830324	1.458068819349915
9	0.21941075208369323	1.1800785522211046
>10	1.079171513418939	11.149713117159504
>50	0.055703236381385954	2.2702332185356946
>100	0.017045236014039785	1.5867166447898415
>500	3.564138774656877E-4	0.13358204110664834
>1k	1.7820693864074532E-4	0.18377736553614676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.533561412944001E-4	0.0	0.0	0.0
6	0.0	3.533561412944001E-4	0.0	0.0	0.0
7	3.533561412944001E-5	3.533561412944001E-4	0.0	0.0	0.0
8	3.533561412944001E-5	3.533561412944001E-4	0.0	0.0	0.0
9	3.533561412944001E-5	3.533561412944001E-4	0.0	0.0	0.0
10	7.067122825888002E-5	3.533561412944001E-4	0.0	3.533561412944001E-5	0.0
11	7.067122825888002E-5	3.533561412944001E-4	0.0	3.533561412944001E-5	0.0
12	1.4134245651776004E-4	3.533561412944001E-4	0.0	3.533561412944001E-5	0.0
13	1.7667807064720005E-4	3.533561412944001E-4	0.0	3.533561412944001E-5	0.0
14	2.1201368477664006E-4	3.533561412944001E-4	0.0	7.067122825888002E-5	0.0
15	2.1201368477664006E-4	3.533561412944001E-4	0.0	7.067122825888002E-5	0.0
16	2.1201368477664006E-4	3.533561412944001E-4	0.0	7.067122825888002E-5	0.0
17	2.1201368477664006E-4	3.533561412944001E-4	0.0	7.067122825888002E-5	0.0
18	2.1201368477664006E-4	3.533561412944001E-4	0.0	7.067122825888002E-5	0.0
19	3.180205271649601E-4	3.533561412944001E-4	0.0	1.0600684238832003E-4	0.0
20	3.180205271649601E-4	4.240273695532801E-4	0.0	1.0600684238832003E-4	0.0
21	3.180205271649601E-4	4.240273695532801E-4	0.0	2.4734929890608007E-4	0.0
22	3.886917554238401E-4	4.240273695532801E-4	0.0	2.826849130355201E-4	0.0
23	3.886917554238401E-4	4.240273695532801E-4	0.0	3.533561412944001E-4	0.0
24	4.240273695532801E-4	4.240273695532801E-4	0.0	4.240273695532801E-4	0.0
25	4.240273695532801E-4	4.240273695532801E-4	0.0	4.240273695532801E-4	0.0
26	4.240273695532801E-4	4.240273695532801E-4	0.0	5.300342119416001E-4	0.0
27	4.240273695532801E-4	4.240273695532801E-4	0.0	8.480547391065602E-4	3.533561412944001E-5
28	4.946985978121601E-4	4.240273695532801E-4	0.0	0.0015547670216953604	3.533561412944001E-5
29	6.007054402004801E-4	4.240273695532801E-4	0.0	0.002826849130355201	3.533561412944001E-5
30	6.007054402004801E-4	4.240273695532801E-4	0.0	0.004805643521603841	3.533561412944001E-5
31	6.007054402004801E-4	4.240273695532801E-4	0.0	0.009257930901913283	3.533561412944001E-5
32	6.007054402004801E-4	4.240273695532801E-4	0.0	0.014876293548494244	3.533561412944001E-5
33	6.007054402004801E-4	4.240273695532801E-4	0.0	0.021378046548311207	3.533561412944001E-5
34	6.360410543299202E-4	4.240273695532801E-4	0.0	0.031484032189331046	3.533561412944001E-5
35	7.773835108476802E-4	5.300342119416001E-4	0.0	0.04367481906398785	3.533561412944001E-5
36	8.127191249771202E-4	5.300342119416001E-4	0.0	0.05992920156353025	3.533561412944001E-5
37	8.127191249771202E-4	6.007054402004801E-4	0.0	0.07954046740536946	3.533561412944001E-5
38	9.540615814948802E-4	6.007054402004801E-4	0.0	0.09950508938850307	3.533561412944001E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4905	0.0	20.677376	1
GGTATCA	2750	0.0	20.0805	1
ATACCGT	160	1.1023076E-9	17.876392	6
ATTCGGT	220	1.8189894E-11	16.00153	26
TATCAAC	6565	0.0	15.248175	2
TGCGATA	180	9.038922E-8	14.670143	13
GCGATAC	195	1.5139449E-8	14.669884	14
TATACTG	660	0.0	14.668328	5
ATCAACG	6785	0.0	14.591888	3
TCAACGC	6790	0.0	14.516852	4
CAACGCA	6880	0.0	14.327205	5
TGTACGG	200	2.1178494E-8	14.300609	2
TATAACG	125	1.6716658E-4	14.0806	2
CGTGCCG	315	0.0	13.971318	10
CTATACT	505	0.0	13.941927	4
AACGCAG	7110	0.0	13.801357	6
GTACATG	7440	0.0	13.66163	1
ATAACGC	145	3.83719E-5	13.655994	3
CGTACAC	245	1.5352271E-9	13.4702	3
GTACCGA	295	9.094947E-12	13.424775	6
>>END_MODULE