FastQCFastQC Report
Mon 6 Feb 2017
SRR2077134_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077134_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051652
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG654.928635E-420.31129522
GGTATCA22700.019.5840971
GTATCAA39700.018.6817461
GTATTAC3150.016.7679021
TAGATCG953.3443014E-416.2131355
GTATAAC2900.015.1778431
TCGATCG1503.2609933E-614.66373841
TCAACGC49700.014.4790784
ATCAACG50150.014.3500993
CAACGCA50400.014.2761085
ATACCGT1851.2704186E-714.2676546
TATCAAC50600.014.26573852
GTACATG77900.014.1822241
AACGCAG52450.013.797286
ACCGTGC5150.013.66739758
GTGTACG2907.2759576E-1213.6600591
TACATGG78050.013.5626052
TAGGACG1302.3265692E-413.5424174
ACGTCGT1302.3291564E-413.54064123
GTATACG1000.00874381513.2047241
Produced by FastQC (version 0.11.5)