##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077134_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3051652
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.13504029948369	31.0	30.0	34.0	23.0	34.0
2	29.16292257439577	31.0	30.0	34.0	22.0	34.0
3	29.225695459377413	31.0	30.0	34.0	22.0	34.0
4	32.59838638219561	35.0	33.0	37.0	24.0	37.0
5	32.55507672565548	37.0	33.0	37.0	24.0	37.0
6	32.63722469010228	37.0	35.0	37.0	25.0	37.0
7	32.44937627226171	37.0	33.0	37.0	22.0	37.0
8	32.390485874536154	37.0	33.0	37.0	22.0	37.0
9	33.74081284497708	38.0	34.0	39.0	21.0	39.0
10	33.6662401217439	38.0	34.0	39.0	20.0	39.0
11	33.7403023018352	38.0	34.0	39.0	22.0	39.0
12	33.63574680206	38.0	34.0	39.0	19.0	39.0
13	33.5880631212209	38.0	34.0	39.0	18.0	39.0
14	34.534005515701004	39.0	34.0	41.0	17.0	41.0
15	34.590941234452686	39.0	34.0	41.0	18.0	41.0
16	34.56411215957783	39.0	34.0	41.0	18.0	41.0
17	34.411829396012386	39.0	34.0	41.0	17.0	41.0
18	34.3958154468465	39.0	34.0	41.0	17.0	41.0
19	34.52121277262283	39.0	34.0	41.0	18.0	41.0
20	34.50200383267817	39.0	34.0	41.0	18.0	41.0
21	34.448126457407334	39.0	34.0	41.0	18.0	41.0
22	34.37849826913423	39.0	34.0	41.0	18.0	41.0
23	34.34170737685686	39.0	34.0	41.0	18.0	41.0
24	34.35023521685959	39.0	34.0	41.0	18.0	41.0
25	34.26117919081206	39.0	34.0	41.0	17.0	41.0
26	34.20895108616579	39.0	34.0	41.0	17.0	41.0
27	34.15394776337538	39.0	34.0	41.0	16.0	41.0
28	34.04891874958219	39.0	34.0	41.0	16.0	41.0
29	34.03453965262094	39.0	34.0	41.0	16.0	41.0
30	33.881232853549484	39.0	33.0	41.0	13.0	41.0
31	33.87645216427037	39.0	33.0	41.0	14.0	41.0
32	33.75033752210278	39.0	33.0	41.0	10.0	41.0
33	33.707039662451685	39.0	33.0	41.0	9.0	41.0
34	33.6225644339525	39.0	33.0	41.0	9.0	41.0
35	33.56056031290593	38.0	33.0	41.0	9.0	41.0
36	33.47662544746255	38.0	33.0	41.0	8.0	41.0
37	33.38993338689995	38.0	33.0	40.0	8.0	41.0
38	33.30624265152121	38.0	33.0	40.0	7.0	41.0
39	33.20604151456326	38.0	33.0	40.0	2.0	41.0
40	33.143020894912	38.0	33.0	40.0	2.0	41.0
41	33.03668275412793	38.0	33.0	40.0	2.0	41.0
42	32.89994534108083	38.0	32.0	40.0	2.0	41.0
43	32.81811654802055	38.0	32.0	40.0	2.0	41.0
44	32.67856852616222	38.0	32.0	40.0	2.0	41.0
45	32.54228365488594	38.0	32.0	40.0	2.0	41.0
46	32.411523659971714	38.0	32.0	40.0	2.0	41.0
47	32.25875132551156	38.0	32.0	40.0	2.0	41.0
48	32.09977874279243	38.0	31.0	40.0	2.0	41.0
49	31.934639008641877	38.0	31.0	40.0	2.0	41.0
50	31.72992792100803	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8334031639856576
1101	2	1.0767209080499711
1101	3	0.9442809705110342
1101	4	1.1085299590217517
1101	5	1.0305408487046535
1101	6	1.1026155145770602
1101	7	0.9876117929644295
1101	8	0.9742614984546663
1101	9	1.1434093667827625
1101	10	1.2794358971417878
1101	11	1.395496481255229
1101	12	1.2622888478520693
1101	13	1.2188147730004744
1101	14	1.4635025875314511
1101	15	1.4836065323991505
1101	16	1.481607476421047
1101	17	1.5556468119063922
1101	18	1.6225643815694468
1101	19	1.5520070006362587
1101	20	1.6362794487435082
1101	21	1.4595319048186113
1101	22	1.5094918768920138
1101	23	1.554125158282929
1101	24	1.6152421581075203
1101	25	1.4749263398611703
1101	26	1.6089638513956288
1101	27	1.5121940352383305
1101	28	1.6225821386040309
1101	29	1.6261930980586854
1101	30	1.7678974910820955
1101	31	1.5358120134198927
1101	32	1.6191272414932598
1101	33	1.893336396899187
1101	34	1.7255746841468635
1101	35	1.7052991979657435
1101	36	1.9044410624564847
1101	37	1.766865248948747
1101	38	1.780622967329542
1101	39	1.8454736685637911
1101	40	1.7314258151459896
1101	41	1.7040713036797968
1101	42	1.8896995052698955
1101	43	1.771987880663751
1101	44	1.6203650032635935
1101	45	1.7481765347120017
1101	46	1.8135194214093602
1101	47	1.8812197185206827
1101	48	1.9200343902386052
1101	49	1.8677921045641952
1101	50	2.0010449021512393
1102	1	0.7452540673354946
1102	2	0.7472278279073201
1102	3	0.7681832641719772
1102	4	0.7679302873955862
1102	5	0.8128802322603406
1102	6	0.7451097718967645
1102	7	0.8357385753836226
1102	8	0.9093433055018991
1102	9	0.9369612169588208
1102	10	1.1083445722737437
1102	11	0.8776998039967623
1102	12	1.0415094201956805
1102	13	1.0078012808394163
1102	14	1.278660045421148
1102	15	1.143672522537642
1102	16	1.1421688914020223
1102	17	1.3012077795729624
1102	18	1.2921576858841988
1102	19	1.1779597964156707
1102	20	1.210499850528393
1102	21	1.2480971647726378
1102	22	1.2531313234578931
1102	23	1.160328862046228
1102	24	1.0664039526943085
1102	25	1.248237534885746
1102	26	1.2628533628060055
1102	27	1.0534399635508862
1102	28	1.3003225186088798
1102	29	1.3025151052195767
1102	30	1.2564855029272337
1102	31	1.3293057770847057
1102	32	1.4057624524100447
1102	33	1.42967666993475
1102	34	1.456910731831222
1102	35	1.4163463191596293
1102	36	1.4231481371682904
1102	37	1.3558940102997568
1102	38	1.2999744644168487
1102	39	1.532785128758924
1102	40	1.562167513923825
1102	41	1.3553483702240925
1102	42	1.3297920382086517
1102	43	1.1748712564651385
1102	44	1.166680065787851
1102	45	1.28221233402423
1102	46	1.2471099658907505
1102	47	1.2663815594479573
1102	48	1.1708360162747482
1102	49	1.2232215360501115
1102	50	1.2664558489324058
1103	1	0.39335695051155994
1103	2	0.43629704285713444
1103	3	0.3439329508971021
1103	4	0.40001845215041243
1103	5	0.4316334537607105
1103	6	0.43767265883024464
1103	7	0.4014414745611603
1103	8	0.43745227719583824
1103	9	0.5643129253148373
1103	10	0.5606792476501852
1103	11	0.45467400120567447
1103	12	0.4968821235709271
1103	13	0.5004237962916278
1103	14	0.6002831223909482
1103	15	0.5826029812751017
1103	16	0.5295311012470734
1103	17	0.8047487857513289
1103	18	0.5327396996023168
1103	19	0.5723239065401415
1103	20	0.48637882662692533
1103	21	0.45553314684512713
1103	22	0.4996297449881695
1103	23	0.5507048568235504
1103	24	0.5512793684655151
1103	25	0.5340140177917121
1103	26	0.5835826926857592
1103	27	0.5796401733712244
1103	28	0.6178646444713465
1103	29	0.5417731228676885
1103	30	0.7532611227171984
1103	31	0.6677568409626176
1103	32	0.7382498790027725
1103	33	0.633278425153712
1103	34	0.6022641475693433
1103	35	0.5516362442438378
1103	36	0.6296820991388259
1103	37	0.7120947165008076
1103	38	0.6111225992924929
1103	39	0.6868835893294332
1103	40	0.6458174034110513
1103	41	0.6524830701472908
1103	42	0.766239231992536
1103	43	0.7559471396719886
1103	44	0.727660017661961
1103	45	0.5661424168108979
1103	46	0.5886684882344824
1103	47	0.7000619543575652
1103	48	0.5300175724429508
1103	49	0.5744122004902508
1103	50	0.7022051251485912
1104	1	0.6184248344426848
1104	2	0.5399348193260742
1104	3	0.5929148312078993
1104	4	0.6136132887598933
1104	5	0.6301524308431823
1104	6	0.5217401637032921
1104	7	0.5963222963394657
1104	8	0.6682887520760588
1104	9	0.6858809288019074
1104	10	0.5568951293591056
1104	11	0.6744017345520206
1104	12	0.5757893131728338
1104	13	0.7020994513813079
1104	14	0.7986213282139119
1104	15	0.7717810848660349
1104	16	0.8191751944592625
1104	17	0.8305498272808052
1104	18	0.7095727217336503
1104	19	0.8191134450720341
1104	20	0.7629339396880752
1104	21	0.8220313655395799
1104	22	0.802720929068883
1104	23	0.8268299696105998
1104	24	0.6589036949466092
1104	25	0.7799488594686679
1104	26	0.6879637438822215
1104	27	0.8127985071112462
1104	28	0.7414632048083192
1104	29	0.6422031454262154
1104	30	0.7703760517446483
1104	31	0.8062160378452958
1104	32	0.8425869407433311
1104	33	0.9241152253865366
1104	34	0.8206257119271001
1104	35	0.800710292938426
1104	36	0.794767663533122
1104	37	0.8990688012738843
1104	38	0.8976737033855002
1104	39	0.9623860752685189
1104	40	0.9814443389526772
1104	41	0.866328071717227
1104	42	0.7920595272656357
1104	43	0.8172187023606412
1104	44	0.9237596192387159
1104	45	0.8999168480622188
1104	46	0.8005083817684095
1104	47	0.8509784570665673
1104	48	0.8017899129694754
1104	49	0.8257579235184664
1104	50	0.8667051885477797
1105	1	0.5229595772844959
1105	2	0.4763258903264713
1105	3	0.5076771720651614
1105	4	0.5364251652208694
1105	5	0.503661509096041
1105	6	0.4117780104424824
1105	7	0.4641914393638018
1105	8	0.48996501510402624
1105	9	0.4679806781451603
1105	10	0.4334684942542708
1105	11	0.4744479998932647
1105	12	0.46287716345058527
1105	13	0.5318915847945007
1105	14	0.7352040095545078
1105	15	0.5854595703204453
1105	16	0.5667179027562881
1105	17	0.6307807314724698
1105	18	0.5425829370787412
1105	19	0.5488021680535482
1105	20	0.6654651186198066
1105	21	0.7076818213799427
1105	22	0.6764585876834843
1105	23	0.6502905115066113
1105	24	0.7516540849803377
1105	25	0.6155556378693063
1105	26	0.6627861029287345
1105	27	0.7529512249887844
1105	28	0.7183554625464126
1105	29	0.6911127938624375
1105	30	0.7242940818128929
1105	31	0.5833290594262834
1105	32	0.782222289576616
1105	33	0.8809988849539039
1105	34	0.7369739610796913
1105	35	0.8165019848239297
1105	36	0.8322966717964491
1105	37	0.8826969969361329
1105	38	0.8648770691362557
1105	39	0.8525477187655426
1105	40	0.9126657333985122
1105	41	0.853263711134872
1105	42	0.9643119416721078
1105	43	0.8977799706647644
1105	44	0.9349262558012228
1105	45	1.086075483255776
1105	46	1.101514917011933
1105	47	0.8729853377157184
1105	48	1.1231141157637765
1105	49	0.963432171152121
1105	50	1.07257476042437
1106	1	0.290672098663439
1106	2	0.18513413889562358
1106	3	0.25105067202254716
1106	4	0.19103552855739991
1106	5	0.26900883882780846
1106	6	0.18644809001724383
1106	7	0.1824363760606147
1106	8	0.3181296985384634
1106	9	0.3691741754712936
1106	10	0.3697148111185484
1106	11	0.2727560122287116
1106	12	0.34009149236898395
1106	13	0.3323190395356761
1106	14	0.4322776491007687
1106	15	0.4204795405492092
1106	16	0.34140564814352103
1106	17	0.5173787510811039
1106	18	0.24183451893433983
1106	19	0.2559744465461833
1106	20	0.3074320972032183
1106	21	0.3832654758283738
1106	22	0.5065986151047923
1106	23	0.3993455767129035
1106	24	0.35423424515588664
1106	25	0.465876146181337
1106	26	0.24770376038407704
1106	27	0.46225556432463577
1106	28	0.43612379465375284
1106	29	0.5075130391152172
1106	30	0.5800043178066403
1106	31	0.42013733116371554
1106	32	0.523221661550771
1106	33	0.38820070300182863
1106	34	0.46316412796576856
1106	35	0.33507098781843325
1106	36	0.5084343377082163
1106	37	0.5066751310457178
1106	38	0.5250241959801016
1106	39	0.5586637719830776
1106	40	0.6145167961191831
1106	41	0.5829893573436777
1106	42	0.46654246769960395
1106	43	0.4812688486498544
1106	44	0.46687071978240624
1106	45	0.44092658983068844
1106	46	0.33207135287013756
1106	47	0.34101418884215207
1106	48	0.480189666693029
1106	49	0.4665900075327727
1106	50	0.3933083839679732
1107	1	0.2389128899631352
1107	2	0.1570402329352092
1107	3	0.16720900218732737
1107	4	0.1418246808447492
1107	5	0.18102592998830858
1107	6	0.14950441470410425
1107	7	0.22450054764782124
1107	8	0.22747703754825466
1107	9	0.3385620632748072
1107	10	0.227113275355741
1107	11	0.1513619637525565
1107	12	0.0793399416751015
1107	13	0.22068192262904063
1107	14	0.1600652201737347
1107	15	0.1639029599159727
1107	16	0.1175036915634351
1107	17	0.17259879054725502
1107	18	0.229061096210728
1107	19	0.12824156042434964
1107	20	0.25880512188410876
1107	21	0.20439370732453455
1107	22	-0.06161305857170163
1107	23	-0.013319800966826278
1107	24	-0.07050026704671097
1107	25	-0.062036831269573156
1107	26	0.22289700682512148
1107	27	0.11436039849706958
1107	28	0.10610824260478324
1107	29	0.28804179902665794
1107	30	0.24812522649366286
1107	31	0.15739857421247194
1107	32	0.09052174326933482
1107	33	0.12890214804028233
1107	34	0.1982158649038439
1107	35	0.22678222689261673
1107	36	0.030652131092409718
1107	37	0.06598534150963076
1107	38	-0.0013564567963086915
1107	39	0.23071642136655157
1107	40	0.17538217084032937
1107	41	0.28775938387823174
1107	42	0.17537940299498445
1107	43	0.03839982967414102
1107	44	0.3191259700822897
1107	45	0.4100780757609357
1107	46	0.23785689552806133
1107	47	0.39250501212539746
1107	48	0.2681361703755627
1107	49	0.30975628245860065
1107	50	0.3275041323593264
1108	1	-0.13405673669645068
1108	2	-0.14048461539035628
1108	3	-0.10375523193890501
1108	4	-0.18599103965241426
1108	5	-0.299979281639267
1108	6	-0.2544936902741597
1108	7	-0.3257966995256396
1108	8	-0.13698676036227653
1108	9	-0.3503649779910134
1108	10	-0.188728079894652
1108	11	-0.147575530641312
1108	12	-0.26841732329655343
1108	13	-0.17345367947613965
1108	14	-0.2364794795612184
1108	15	-0.09985161903795614
1108	16	-0.19505381474304073
1108	17	-0.26889817334222244
1108	18	-0.22713058859087454
1108	19	-0.2281708182974782
1108	20	-0.3258172279793925
1108	21	-0.1710082558487045
1108	22	-0.2587407643027575
1108	23	-0.47047726994807704
1108	24	-0.2201580039703046
1108	25	-0.03918494431589892
1108	26	-0.16141116921804155
1108	27	-0.2645119046701794
1108	28	-0.3200206288840306
1108	29	-0.5293030365345999
1108	30	-0.3418175565934831
1108	31	-0.3433504726159029
1108	32	-0.15026789255391293
1108	33	-0.13639080530247583
1108	34	-0.10833329749130627
1108	35	-0.2512212359791519
1108	36	-0.48843839353671115
1108	37	-0.2793678311303296
1108	38	-0.09794516342967086
1108	39	-0.12668247776978347
1108	40	-0.09032777150101623
1108	41	-0.08084602011169295
1108	42	0.0015187856097398367
1108	43	-0.11035651662670176
1108	44	-0.23880884552372095
1108	45	-0.13462221533289664
1108	46	-0.09614080505389921
1108	47	-0.14223922469098937
1108	48	-0.17121920125867973
1108	49	-0.17316099275564767
1108	50	-0.19991862456997467
1109	1	0.11186976517166869
1109	2	0.16060086287252773
1109	3	0.10390754675270486
1109	4	-0.006052859457668092
1109	5	0.03086382641984642
1109	6	-0.0383703706263816
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2211	4	-0.12376182455408724
2211	5	-0.26694733452549
2211	6	-0.15725140420828154
2211	7	-0.07647973418271903
2211	8	0.033740877874485875
2211	9	0.0026187409633209313
2211	10	-0.20411191118940053
2211	11	-0.08197071392279298
2211	12	-0.1365804712671732
2211	13	-0.05094311445743216
2211	14	-0.4079880037906207
2211	15	-0.09908228119663676
2211	16	-0.21608815648147583
2211	17	-0.0909587146025359
2211	18	-0.2854382054743354
2211	19	-0.09401525145724321
2211	20	-0.23087841316462487
2211	21	-0.2308841116822009
2211	22	-0.27410180724555744
2211	23	-0.4429305407386792
2211	24	-0.273463885551557
2211	25	-0.2049131568807141
2211	26	-0.2457410823482249
2211	27	-0.18402821882054354
2211	28	-0.01911229749335064
2211	29	-0.13250416868456227
2211	30	-0.5222715098028416
2211	31	-0.4198187892619032
2211	32	-0.12192464885373511
2211	33	-0.3113431422325661
2211	34	-0.23671224807537783
2211	35	-0.09292341192801956
2211	36	-0.18716198406944784
2211	37	-0.11914138882012537
2211	38	-0.1641818949887366
2211	39	-0.11917972084112449
2211	40	-0.18949906076434786
2211	41	-0.16417255286702215
2211	42	-0.2192760936628062
2211	43	-0.11851543951369337
2211	44	-0.3289607206509402
2211	45	-0.1908690261184418
2211	46	-0.3152571220374085
2211	47	-0.3553322569060171
2211	48	-0.2841808309091576
2211	49	-0.1666592295839031
2211	50	-0.09433772638247717
2212	1	-0.18453039639017632
2212	2	-0.3167297592639109
2212	3	-0.25953584801935037
2212	4	-0.31911470311266754
2212	5	-0.279710920600202
2212	6	-0.35767963206674835
2212	7	-0.18976950675490656
2212	8	-0.07457544664459448
2212	9	-0.2066074161162419
2212	10	-0.24634921978337587
2212	11	-0.25156289664143117
2212	12	-0.27148634908879643
2212	13	-0.283758045416306
2212	14	-1.0647012033381316
2212	15	-0.5845821280414896
2212	16	-0.5795840061286128
2212	17	-0.3728657843048069
2212	18	-0.29932710278760055
2212	19	-0.32222324524612134
2212	20	-0.2555236542854118
2212	21	-0.41732305736822894
2212	22	-0.3568535048414816
2212	23	-0.506300378576185
2212	24	-0.3968342996163585
2212	25	-0.22283791979351264
2212	26	-0.3035871750722947
2212	27	-0.3576266975804998
2212	28	-0.3190912197056974
2212	29	-0.32663210933921505
2212	30	-0.42100320321353024
2212	31	-0.2815604817324342
2212	32	-0.29287020750609116
2212	33	-0.19730442582434193
2212	34	-0.24933268716057455
2212	35	-0.3046787518869536
2212	36	-0.4784224357709874
2212	37	-0.4035479202048009
2212	38	-0.45090319204603446
2212	39	-0.5040551192719605
2212	40	-0.401995004427512
2212	41	-0.3580480149861458
2212	42	-0.3469061921370198
2212	43	-0.36622084179615655
2212	44	-0.323215279670265
2212	45	-0.37538200791269105
2212	46	-0.21747317087721996
2212	47	-0.27592540707994573
2212	48	-0.3118420153291872
2212	49	-0.33995746359890333
2212	50	-0.22016984407841989
2213	1	-0.5023724450749647
2213	2	-0.6219057517321609
2213	3	-0.5441762880896626
2213	4	-0.8604468170630213
2213	5	-0.5007206212950379
2213	6	-0.5400598641035472
2213	7	-0.6410652212888017
2213	8	-0.5653223478009863
2213	9	-0.5568648976073831
2213	10	-0.6516577461835737
2213	11	-0.7384068745408143
2213	12	-0.6934812161489887
2213	13	-0.6641716620450282
2213	14	-0.6802506881096306
2213	15	-0.8751251833620799
2213	16	-0.7122349199451392
2213	17	-0.7589006376184955
2213	18	-0.7041317423097198
2213	19	-0.8291496035671955
2213	20	-0.8337242501191326
2213	21	-0.7764366428169609
2213	22	-0.6725217922102473
2213	23	-0.6589774577923322
2213	24	-0.6557174489927959
2213	25	-0.7805760579307446
2213	26	-0.825362189050189
2213	27	-0.8929166828856978
2213	28	-0.921367245326401
2213	29	-1.0441961933264352
2213	30	-1.1254484762584624
2213	31	-0.9819883910874125
2213	32	-1.032409623643673
2213	33	-1.0266014042479412
2213	34	-1.0785038455921097
2213	35	-1.0664741201029386
2213	36	-1.253811521863419
2213	37	-1.0409451343369938
2213	38	-0.8579515771727984
2213	39	-1.0473387517486188
2213	40	-0.9247133459250492
2213	41	-0.965143511487689
2213	42	-0.8795886273323248
2213	43	-1.030910221557079
2213	44	-0.8367983766248663
2213	45	-0.894461583652383
2213	46	-0.9103981955139666
2213	47	-1.0375425714000244
2213	48	-1.05612625243975
2213	49	-1.1437439739660604
2213	50	-1.024116161387564
2214	1	-0.48346900342700394
2214	2	-0.7789116370588722
2214	3	-0.739416092291691
2214	4	-0.7020493996361736
2214	5	-0.5379149889114174
2214	6	-0.6677667951929465
2214	7	-0.593467576799803
2214	8	-0.6884414501808251
2214	9	-0.820964404661666
2214	10	-0.6098108474971227
2214	11	-0.7195344900259002
2214	12	-0.7640205922802039
2214	13	-0.7124508380647754
2214	14	-0.8363110562746883
2214	15	-0.7144864761670036
2214	16	-0.6692992962057573
2214	17	-0.5718685435222852
2214	18	-0.7610939751105406
2214	19	-0.8521741542308661
2214	20	-0.6662538363518493
2214	21	-0.8738128326933676
2214	22	-0.8331794787644071
2214	23	-0.8843884040514141
2214	24	-1.0368868190678668
2214	25	-0.9935529358951882
2214	26	-0.9932812058503728
2214	27	-1.0756102680348647
2214	28	-0.8858603152722395
2214	29	-0.784388609519624
2214	30	-0.9077500513727301
2214	31	-0.899023573642971
2214	32	-0.9310236128116358
2214	33	-0.9596094428692155
2214	34	-0.974865329916021
2214	35	-1.0510775463948718
2214	36	-0.9019367925349613
2214	37	-0.8849184113994042
2214	38	-0.8189369164806024
2214	39	-0.7937451826456368
2214	40	-0.8520405172931333
2214	41	-0.7478660832943689
2214	42	-0.6466816969330864
2214	43	-0.8182456403193825
2214	44	-0.6563202002997173
2214	45	-0.678945785061341
2214	46	-0.7607114412086737
2214	47	-0.8108444795157688
2214	48	-0.6984214090798488
2214	49	-0.5307822794888821
2214	50	-0.665261341406783
2215	1	-0.427838431272086
2215	2	-0.42620429932217263
2215	3	-0.4912040480798723
2215	4	-0.34281740606320454
2215	5	-0.341765841571835
2215	6	-0.43592276006794606
2215	7	-0.4198522488910399
2215	8	-0.21886526113959803
2215	9	-0.3296451559446396
2215	10	-0.4412488257877598
2215	11	-0.4842646856688546
2215	12	-0.36633674384856363
2215	13	-0.3273877873476465
2215	14	-0.38863819460542715
2215	15	-0.41126883580922424
2215	16	-0.48418648013988275
2215	17	-0.4085133706613675
2215	18	-0.4517884291681895
2215	19	-0.5398232953743403
2215	20	-0.5579831467065759
2215	21	-0.4853065578465845
2215	22	-0.5029473240925526
2215	23	-0.653534462100815
2215	24	-0.5989509944546825
2215	25	-0.6861255671597988
2215	26	-0.7124251783795401
2215	27	-0.6309094238223238
2215	28	-0.5799021048662212
2215	29	-0.6273808232751676
2215	30	-0.5504717217716717
2215	31	-0.6286244655202324
2215	32	-0.6613392487426708
2215	33	-0.685419146099683
2215	34	-0.6606428677750102
2215	35	-0.6184316136278127
2215	36	-0.6552700734784125
2215	37	-0.5586056746437436
2215	38	-0.6027645080341841
2215	39	-0.5242665747938631
2215	40	-0.6698317881517397
2215	41	-0.6982260924464754
2215	42	-0.669375557373094
2215	43	-0.700599328229444
2215	44	-0.649789046103308
2215	45	-0.6290104966384256
2215	46	-0.7017550687807024
2215	47	-0.7048522962874024
2215	48	-0.782630862410592
2215	49	-0.8632883312756761
2215	50	-0.8398433381684853
2216	1	-0.592640644380424
2216	2	-0.5532456107406887
2216	3	-0.6618923548244062
2216	4	-0.588566530536248
2216	5	-0.716000767278171
2216	6	-0.6519756384070305
2216	7	-0.6476215970073476
2216	8	-0.583957409546489
2216	9	-0.686741804501942
2216	10	-0.7984154159116343
2216	11	-0.9396059737944711
2216	12	-0.8990911653343616
2216	13	-0.9414590246406789
2216	14	-0.96136477148913
2216	15	-1.0632961039042073
2216	16	-0.9825096900208479
2216	17	-0.9404406741829376
2216	18	-0.8982303995148513
2216	19	-1.030826530918361
2216	20	-1.139075285054453
2216	21	-1.2067822651758675
2216	22	-1.1173376206667953
2216	23	-1.143745629049917
2216	24	-1.2436868149415545
2216	25	-1.302555721311009
2216	26	-1.2040225243092664
2216	27	-1.3082857160517065
2216	28	-1.3700656172468157
2216	29	-1.3654083202126728
2216	30	-1.344939548179532
2216	31	-1.471859402857298
2216	32	-1.3504544863538186
2216	33	-1.4631365174158475
2216	34	-1.3529974215368128
2216	35	-1.3898269746001688
2216	36	-1.4043351354812188
2216	37	-1.5367623733012117
2216	38	-1.608905770509324
2216	39	-1.5985519841657805
2216	40	-1.751956389759858
2216	41	-1.5561377567194121
2216	42	-1.5156319703860603
2216	43	-1.7695865460545122
2216	44	-1.9121747545107866
2216	45	-2.0718423819455722
2216	46	-2.078104115862221
2216	47	-1.9122615757886763
2216	48	-2.0522401200963856
2216	49	-2.028181547006586
2216	50	-2.092783870233628
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	163369.0
3	7948.0
4	14560.0
5	11133.0
6	9080.0
7	7866.0
8	6942.0
9	6393.0
10	6166.0
11	6276.0
12	6445.0
13	6679.0
14	7330.0
15	7743.0
16	8166.0
17	8912.0
18	9483.0
19	10970.0
20	13254.0
21	16881.0
22	20604.0
23	23406.0
24	25722.0
25	27849.0
26	31109.0
27	35169.0
28	40259.0
29	46432.0
30	54838.0
31	65168.0
32	79929.0
33	99254.0
34	127794.0
35	166913.0
36	221702.0
37	305121.0
38	483267.0
39	861520.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.301620420399544	16.783972697938147	12.725231023627265	25.18917585803505
2	16.374647247976636	18.717045318877336	38.82968325459414	26.07862417855189
3	20.177442940168113	22.831608302975553	27.496505001243175	29.494443755613155
4	13.567840514648335	15.222039187746795	35.333690217758544	35.87643007984633
5	15.265985535529788	36.37732259704479	33.16306148219821	15.193630385227216
6	34.88085854764019	34.44292239838321	16.389113662086537	14.287105391890067
7	30.503245587662995	30.547014056071507	19.90803048121382	19.041709875051676
8	27.310617710107277	34.518525270316616	18.76838969396795	19.402467325608157
9	26.706185044767206	16.008517031275566	18.19495725910025	39.09034066485698
10	15.421824119718366	28.581684975440595	32.257580173350085	23.73891073149095
11	36.571101669354945	22.26579199286623	21.844224136800555	19.318882200978273
12	24.29491710808801	23.992818351237183	28.653250692787797	23.059013847887012
13	29.026547105843196	20.82482149314643	24.831033620170462	25.317597780839908
14	22.915465907623027	20.37572529124759	25.5327435926186	31.176065208510785
15	24.773896069237342	27.958301012476124	22.380010661784713	24.88779225650182
16	24.859011940994353	27.111050493413124	23.90346354736153	24.126474018230994
17	23.82716741533727	27.006009364366037	25.261133094191518	23.905690126105178
18	23.852905101620276	26.631532597537532	25.752623497925104	23.762938802917084
19	24.719292332067237	26.175227687658698	25.793955784525636	23.311524195748433
20	24.819435927872743	25.77949311551313	25.691153950833055	23.709917005781065
21	24.970919660211766	25.540950070360157	25.643792974872028	23.84433729455605
22	24.774468983828143	25.34943041694214	25.511995088625127	24.36410551060458
23	24.53122986646662	25.35973552344646	25.584034355984315	24.52500025410261
24	24.25219470123533	25.6347662975218	25.578591128061678	24.534447873181193
25	24.545715717475318	25.387774859700535	25.70018549176306	24.366323931061082
26	24.395470261580556	25.65632900453912	25.791672829341834	24.156527904538493
27	24.43924931766138	25.719124074952802	25.5065275940919	24.33509901329392
28	24.351762291465974	25.725037333834504	25.54680578829412	24.3763945864054
29	24.426416442045422	25.80467642898629	25.475476082797922	24.293431046170365
30	24.47984373995663	25.83777263848861	25.52855169555003	24.153831926004727
31	24.534061807281176	25.47224960811848	25.492118271662335	24.50157031293801
32	24.39475464957996	25.782317564147288	25.412633009067044	24.410294777205717
33	24.506485441935276	25.60239759060636	25.348697829749035	24.54241913770933
34	24.645918170304828	25.58332811511852	25.464188894349345	24.306564820227308
35	24.711239693103753	25.63185630525167	25.442447773310413	24.214456228334168
36	24.55096299147516	25.657805652212257	25.406430828317255	24.384800527995328
37	24.611926792002464	25.299153425969035	25.63932405227644	24.449595729752062
38	24.514534460578545	25.59660331485627	25.564901182453475	24.323961042111712
39	24.68767591538292	25.659605844628253	25.412926782808658	24.239791457180168
40	24.744380569185605	25.406371453245562	25.63004050436027	24.219207473208566
41	24.557938937729045	25.582385679119678	25.493545657706278	24.366129725444996
42	24.631523529978686	25.58132445007989	25.511205579088774	24.27594644085265
43	24.45935929868569	25.33764125958326	25.61071115859329	24.592288283137755
44	24.49981003680926	25.58241993108876	25.454313606773244	24.46345642532873
45	24.579190355458895	25.576946714762634	25.312757613054742	24.53110531672373
46	24.570453887686895	25.435527849822666	25.39000109146224	24.6040171710282
47	24.604420966303383	25.472559321695005	25.398742158155567	24.524277553846048
48	24.417494057600305	25.673362709917807	25.404561717704222	24.504581514777662
49	24.648504538237752	25.335795505945008	25.334713880596425	24.680986075220815
50	24.655441014851544	25.4933497305247	25.485745489324728	24.36546376529903
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1673.0
1	1253.5
2	834.0
3	811.5
4	789.0
5	778.0
6	767.0
7	733.0
8	699.0
9	785.5
10	872.0
11	1073.5
12	1275.0
13	1943.5
14	2612.0
15	3538.5
16	4465.0
17	4897.0
18	5329.0
19	5558.0
20	5787.0
21	6368.5
22	6950.0
23	8566.5
24	10183.0
25	12808.5
26	15434.0
27	19717.0
28	24000.0
29	29979.5
30	35959.0
31	44326.0
32	52693.0
33	63001.5
34	73310.0
35	85804.5
36	98299.0
37	110348.0
38	122397.0
39	135266.5
40	148136.0
41	161889.5
42	175643.0
43	185975.5
44	196308.0
45	207419.5
46	218531.0
47	227280.0
48	236029.0
49	239332.5
50	242636.0
51	242394.5
52	242153.0
53	237389.0
54	232625.0
55	222365.5
56	212106.0
57	198868.0
58	185630.0
59	168910.0
60	152190.0
61	133980.0
62	115770.0
63	99629.5
64	83489.0
65	69848.5
66	56208.0
67	45938.0
68	35668.0
69	29155.5
70	22643.0
71	17887.5
72	13132.0
73	10670.5
74	8209.0
75	6339.5
76	4470.0
77	3487.5
78	2505.0
79	2004.0
80	1503.0
81	1125.5
82	748.0
83	602.5
84	457.0
85	339.5
86	222.0
87	178.5
88	135.0
89	102.0
90	69.0
91	49.0
92	29.0
93	26.0
94	23.0
95	21.5
96	20.0
97	17.0
98	14.0
99	19.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08208668616211809
2	0.09070496898073568
3	0.09863509993931155
4	0.09981478884224021
5	0.0918191196112794
6	0.054527842624257285
7	0.049514164786810554
8	0.05603522288911056
9	0.05777198710731105
10	0.04237049309685377
11	0.04495925485605829
12	0.054265689534717595
13	0.04879324379057638
14	0.04908816601630854
15	0.049350319105848243
16	0.05148031295835829
17	0.05174246604789799
18	0.05311876976798141
19	0.060950593317979904
20	0.06691457610500803
21	0.07671254782655428
22	0.0787770034066794
23	0.055805838935763324
24	0.07412378606734975
25	0.0806120750334573
26	0.07802331327425277
27	0.0725180983939191
28	0.09204850356462664
29	0.08012053799057035
30	0.07785946759329046
31	0.053184308040366335
32	0.04862939810961407
33	0.052102926546015074
34	0.04715478698095327
35	0.05344646112990603
36	0.05324984631275125
37	0.056100761161495476
38	0.04535248449036784
39	0.05030062405542965
40	0.05275830926986432
41	0.04014219183576633
42	0.03663589426317287
43	0.03771727575752412
44	0.03476805350020251
45	0.02644469290731709
46	0.022905626198531155
47	0.02854191762363467
48	0.02280731878995377
49	0.02254516570041407
50	0.02369208546715025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3051652.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.88611985599606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.84153018321773	48.17310266692748
2	12.211850966972012	15.114881452111828
3	4.309384806518717	8.000733139254772
4	1.9578779456326416	4.846618768273319
5	1.0711363376698733	3.3144235887575224
6	0.6232214504106062	2.3141254426163083
7	0.4162230567567906	1.8030900984095857
8	0.2954679259319402	1.4628290782261295
9	0.22468271411669843	1.2514267237856844
>10	0.9923962569484666	10.55611178306797
>50	0.043008259562582266	1.7958202684560978
>100	0.012901218443604636	1.2010704586892402
>500	2.657315153187267E-4	0.12133189176251663
>1k	5.314630303881685E-5	0.04443463966160227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.2769136192462314E-5	0.0	0.0	0.0
3	0.0	3.2769136192462314E-5	0.0	0.0	0.0
4	0.0	3.2769136192462314E-5	0.0	0.0	0.0
5	3.2769136192462314E-5	3.2769136192462314E-5	0.0	0.0	0.0
6	3.2769136192462314E-5	3.2769136192462314E-5	0.0	0.0	0.0
7	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
8	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
9	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
10	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
11	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
12	6.553827238492463E-5	3.2769136192462314E-5	0.0	0.0	0.0
13	9.830740857738694E-5	3.2769136192462314E-5	0.0	0.0	0.0
14	1.6384568096231155E-4	3.2769136192462314E-5	0.0	0.0	0.0
15	1.6384568096231155E-4	3.2769136192462314E-5	0.0	0.0	0.0
16	1.6384568096231155E-4	3.2769136192462314E-5	0.0	0.0	0.0
17	1.6384568096231155E-4	3.2769136192462314E-5	0.0	3.2769136192462314E-5	0.0
18	1.6384568096231155E-4	3.2769136192462314E-5	0.0	3.2769136192462314E-5	3.2769136192462314E-5
19	3.276913619246231E-4	3.2769136192462314E-5	0.0	6.553827238492463E-5	3.2769136192462314E-5
20	3.276913619246231E-4	3.2769136192462314E-5	0.0	6.553827238492463E-5	3.2769136192462314E-5
21	3.276913619246231E-4	3.2769136192462314E-5	0.0	6.553827238492463E-5	3.2769136192462314E-5
22	3.9322963430954774E-4	3.2769136192462314E-5	0.0	1.6384568096231155E-4	3.2769136192462314E-5
23	3.9322963430954774E-4	3.2769136192462314E-5	0.0	1.6384568096231155E-4	6.553827238492463E-5
24	5.24306179079397E-4	3.2769136192462314E-5	0.0	2.293839533472362E-4	6.553827238492463E-5
25	5.24306179079397E-4	3.2769136192462314E-5	0.0	3.276913619246231E-4	6.553827238492463E-5
26	5.24306179079397E-4	3.2769136192462314E-5	0.0	4.587679066944724E-4	6.553827238492463E-5
27	5.24306179079397E-4	3.2769136192462314E-5	0.0	6.881518600417086E-4	6.553827238492463E-5
28	6.881518600417086E-4	3.2769136192462314E-5	0.0	0.0017367642182005026	6.553827238492463E-5
29	9.830740857738694E-4	3.2769136192462314E-5	0.0	0.0026870691677819097	6.553827238492463E-5
30	0.0010158432219663316	3.2769136192462314E-5	0.0	0.004784293884099498	6.553827238492463E-5
31	0.0010158432219663316	3.2769136192462314E-5	0.0	0.010289508764433165	9.830740857738694E-5
32	0.0010158432219663316	3.2769136192462314E-5	0.0	0.01753148786296734	9.830740857738694E-5
33	0.001048612358158794	3.2769136192462314E-5	0.0	0.024707928689116584	9.830740857738694E-5
34	0.001048612358158794	3.2769136192462314E-5	0.0	0.03594774240313116	1.3107654476984926E-4
35	0.001245227175313568	3.2769136192462314E-5	0.0	0.049907394421120105	1.3107654476984926E-4
36	0.0012779963115060302	3.2769136192462314E-5	0.0	0.06596427115542663	1.3107654476984926E-4
37	0.0012779963115060302	3.2769136192462314E-5	0.0	0.08801789981295377	1.3107654476984926E-4
38	0.0013107654476984924	3.2769136192462314E-5	0.0	0.11347951863449698	1.3107654476984926E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	65	4.928635E-4	20.311295	22
GGTATCA	2270	0.0	19.584097	1
GTATCAA	3970	0.0	18.681746	1
GTATTAC	315	0.0	16.767902	1
TAGATCG	95	3.3443014E-4	16.213135	5
GTATAAC	290	0.0	15.177843	1
TCGATCG	150	3.2609933E-6	14.663738	41
TCAACGC	4970	0.0	14.479078	4
ATCAACG	5015	0.0	14.350099	3
CAACGCA	5040	0.0	14.276108	5
ATACCGT	185	1.2704186E-7	14.267654	6
TATCAAC	5060	0.0	14.2657385	2
GTACATG	7790	0.0	14.182224	1
AACGCAG	5245	0.0	13.79728	6
ACCGTGC	515	0.0	13.6673975	8
GTGTACG	290	7.2759576E-12	13.660059	1
TACATGG	7805	0.0	13.562605	2
TAGGACG	130	2.3265692E-4	13.542417	4
ACGTCGT	130	2.3291564E-4	13.540641	23
GTATACG	100	0.008743815	13.204724	1
>>END_MODULE