FastQCFastQC Report
Mon 6 Feb 2017
SRR2077134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3051652
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47350.019.2381251
GGTATCA27200.018.9290731
ACGTAAG1154.276939E-617.217523
TGCGATA1501.8356877E-716.13687313
TACCGAT1552.6689486E-715.6140267
CGTGCCG2550.015.53180210
ACCGTGC5850.015.4199138
TATCAAC60950.014.8015362
TCAACGC61650.014.2408684
ATCAACG62500.014.1528093
CAACGCA61850.014.05205255
GTACATG74200.013.8186111
GTATTAA5650.013.6301921
AACGCAG64550.013.4972636
TCTATAC4650.013.2495663
TACATGG75250.013.2168672
GTATATA7450.012.9950751
ACCGATC3600.012.8342558
GCGATAC1557.16659E-512.77657814
GGACCGT1953.5507255E-612.4109456
Produced by FastQC (version 0.11.5)