FastQCFastQC Report
Mon 6 Feb 2017
SRR2077133_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077133_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178360
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.015.8990831
CGTGCCG2550.015.52700510
GTATCAA44300.015.3490361
GTACATG79250.013.8001391
TACATGG78450.013.4355432
TAGACTG7850.013.1732525
GTGTAGC13400.013.1374651
GTCCTAT5350.012.7507081
TATCAAC53250.012.4795822
TCAACGC53750.012.4048214
CAACGCA53850.012.3800315
ATCAACG54100.012.3652433
ACATGGG83150.012.2001473
TATACTG7650.012.0796025
TACACAG19550.012.04208855
GCACCGT5300.012.0366296
TAGCCCT11400.011.9677694
GTGCCGA3501.6370905E-1111.94100711
GTATAAC5000.011.8828361
AACGCAG56000.011.8239136
Produced by FastQC (version 0.11.5)