##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077133_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3178360
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.33181986936659	31.0	30.0	34.0	24.0	34.0
2	29.370484778313344	31.0	30.0	34.0	23.0	34.0
3	29.492372166777834	31.0	30.0	34.0	24.0	34.0
4	32.92422412816673	37.0	35.0	37.0	26.0	37.0
5	32.877224732251854	37.0	35.0	37.0	26.0	37.0
6	32.93904088901194	37.0	35.0	37.0	26.0	37.0
7	32.81536924703306	37.0	35.0	37.0	25.0	37.0
8	32.743767855120254	37.0	35.0	37.0	25.0	37.0
9	34.137482538164335	39.0	35.0	39.0	25.0	39.0
10	34.08633981046829	38.0	35.0	39.0	25.0	39.0
11	34.10107665588543	38.0	35.0	39.0	25.0	39.0
12	34.01201657458564	38.0	35.0	39.0	25.0	39.0
13	33.95618557998465	38.0	35.0	39.0	23.0	39.0
14	34.92073301954467	39.0	34.0	41.0	22.0	41.0
15	34.964345763223804	39.0	35.0	41.0	23.0	41.0
16	34.93263821593526	39.0	35.0	41.0	23.0	41.0
17	34.77317358637788	39.0	34.0	41.0	22.0	41.0
18	34.75228545539209	39.0	34.0	41.0	22.0	41.0
19	34.87986540228294	39.0	34.0	41.0	22.0	41.0
20	34.86853156974037	39.0	34.0	41.0	22.0	41.0
21	34.819836330686265	39.0	34.0	41.0	22.0	41.0
22	34.74394058571087	39.0	34.0	41.0	22.0	41.0
23	34.70605154859739	39.0	34.0	41.0	22.0	41.0
24	34.71124101738003	39.0	34.0	41.0	22.0	41.0
25	34.62237726374608	39.0	34.0	41.0	20.0	41.0
26	34.57217527278219	39.0	34.0	41.0	19.0	41.0
27	34.51577008268415	39.0	34.0	41.0	19.0	41.0
28	34.41335437143684	39.0	34.0	41.0	18.0	41.0
29	34.402443083854564	39.0	34.0	41.0	18.0	41.0
30	34.25004625026743	39.0	34.0	41.0	17.0	41.0
31	34.252017392617574	39.0	34.0	41.0	18.0	41.0
32	34.128018852489966	39.0	34.0	41.0	16.0	41.0
33	34.08303181514995	39.0	34.0	41.0	16.0	41.0
34	34.014711989831234	39.0	33.0	41.0	16.0	41.0
35	33.952748587321764	39.0	33.0	41.0	16.0	41.0
36	33.86869674926692	38.0	33.0	41.0	15.0	41.0
37	33.77651398834619	38.0	33.0	40.0	15.0	41.0
38	33.69573239028933	38.0	33.0	40.0	13.0	41.0
39	33.58580368491927	38.0	33.0	40.0	9.0	41.0
40	33.52845461181238	38.0	33.0	40.0	9.0	41.0
41	33.42655457531557	38.0	33.0	40.0	9.0	41.0
42	33.285306573201275	38.0	33.0	40.0	8.0	41.0
43	33.20347915277061	38.0	33.0	40.0	7.0	41.0
44	33.05853364628299	38.0	33.0	40.0	2.0	41.0
45	32.91810524924804	38.0	32.0	40.0	2.0	41.0
46	32.790274858732175	38.0	32.0	40.0	2.0	41.0
47	32.6415110308461	38.0	32.0	40.0	2.0	41.0
48	32.48324764973131	38.0	32.0	40.0	2.0	41.0
49	32.317894448709396	38.0	32.0	40.0	2.0	41.0
50	32.11185831686782	38.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.639463439076252
1101	2	1.7674690446484682
1101	3	1.8073175274471325
1101	4	2.0135998787909486
1101	5	2.0812493600659536
1101	6	2.005139938908144
1101	7	2.0384874925989678
1101	8	2.1588346012503763
1101	9	2.514410105799925
1101	10	2.460385767951749
1101	11	2.4123702671386695
1101	12	2.53139629038224
1101	13	2.480098974208346
1101	14	2.915930348304798
1101	15	2.8952624206207105
1101	16	2.9349925087731563
1101	17	3.0425269023258537
1101	18	3.028976163137031
1101	19	2.8600954180878517
1101	20	2.9846681557564168
1101	21	2.9751221876780107
1101	22	2.9601001991667957
1101	23	2.9976856470593916
1101	24	2.9755280336533687
1101	25	2.9203072244291306
1101	26	3.022103094492337
1101	27	3.1652457105652445
1101	28	3.137308759237051
1101	29	3.128779403585561
1101	30	3.1685295028554563
1101	31	3.2668552142012857
1101	32	3.276376159126073
1101	33	3.303353439798663
1101	34	3.330675577061953
1101	35	3.26845469062922
1101	36	3.2534023557555756
1101	37	3.341590506877438
1101	38	3.4172577518982976
1101	39	3.35817359819238
1101	40	3.412361320369989
1101	41	3.4425364444199857
1101	42	3.582820840500659
1101	43	3.54901111089346
1101	44	3.4023388186010806
1101	45	3.313820614589659
1101	46	3.4444938172728996
1101	47	3.3271053810641504
1101	48	3.3398613374725628
1101	49	3.4363829982586154
1101	50	3.435290710784436
1102	1	1.055494428378669
1102	2	1.0217347971069728
1102	3	1.1281697728312068
1102	4	1.2369109504334332
1102	5	1.1767406876016508
1102	6	1.1900197945261652
1102	7	1.2995816143948034
1102	8	1.3082127230679674
1102	9	1.3512332967346055
1102	10	1.5474185432343006
1102	11	1.326634812787006
1102	12	1.4928223838932126
1102	13	1.4476869711952745
1102	14	1.7583961186676191
1102	15	1.5974708832286382
1102	16	1.6489508016115764
1102	17	1.8972665256131407
1102	18	1.8612082821918179
1102	19	1.7900734729373724
1102	20	1.8496711060116056
1102	21	1.8176678350253255
1102	22	1.836682839202524
1102	23	1.7617658821301916
1102	24	1.6231802685520407
1102	25	1.8482557114403448
1102	26	1.9365716603023202
1102	27	1.7216873318652475
1102	28	1.8535327887069641
1102	29	1.7208100545854563
1102	30	1.8339530066705123
1102	31	1.9479857974912207
1102	32	1.9887735122550438
1102	33	2.0011948817587637
1102	34	1.9744922329217331
1102	35	1.9675177805875208
1102	36	2.0150724326501575
1102	37	1.9861305069466795
1102	38	1.9617912727818023
1102	39	1.9902231130200647
1102	40	2.0548425715353744
1102	41	1.9541213274410083
1102	42	1.9052036080428039
1102	43	1.7780231240951707
1102	44	1.655888151170764
1102	45	1.8213426524765737
1102	46	1.852090736741573
1102	47	1.7684647933673858
1102	48	1.6593982092326627
1102	49	1.7524077556724578
1102	50	1.7678759701917457
1103	1	0.3153660769525324
1103	2	0.43279889206058186
1103	3	0.4175460975242551
1103	4	0.486076724720256
1103	5	0.5534327264022991
1103	6	0.481076403980488
1103	7	0.4350601239606533
1103	8	0.5280378776149846
1103	9	0.6586314245302276
1103	10	0.5424346353498564
1103	11	0.5757020166138815
1103	12	0.5786518799322167
1103	13	0.6060177731708833
1103	14	0.6018254825636475
1103	15	0.5860075291149869
1103	16	0.49677265063237286
1103	17	0.6813056258795456
1103	18	0.4690142330661544
1103	19	0.584821521718041
1103	20	0.5698689008147468
1103	21	0.4248932449283913
1103	22	0.439603355057848
1103	23	0.583509256865554
1103	24	0.5174307560976388
1103	25	0.5658694474779224
1103	26	0.6418635520768348
1103	27	0.6053976606099312
1103	28	0.5355309680812894
1103	29	0.6011218007440746
1103	30	0.8673819582297142
1103	31	0.6518686196610801
1103	32	0.8306228697537605
1103	33	0.6637190028530568
1103	34	0.7188246632618132
1103	35	0.681903724439934
1103	36	0.8240669383229644
1103	37	0.777740599293054
1103	38	0.8142346612536002
1103	39	0.9002098377261945
1103	40	0.7832919337674227
1103	41	0.9097329510453207
1103	42	0.9220403209521919
1103	43	0.8743735528156762
1103	44	0.8318615039644399
1103	45	0.8149848578100602
1103	46	0.7768136964123471
1103	47	0.8244138256079765
1103	48	0.7557733781626652
1103	49	0.7590317393780808
1103	50	0.7643964329556567
1104	1	0.38107733787710885
1104	2	0.4411444190412048
1104	3	0.40233382255892636
1104	4	0.3763232271115058
1104	5	0.3367486518331759
1104	6	0.32631568757022933
1104	7	0.43635678951548584
1104	8	0.4231740528594017
1104	9	0.4127137196789832
1104	10	0.3172404905729138
1104	11	0.47099112981719315
1104	12	0.33192306421332773
1104	13	0.3290241219830463
1104	14	0.5952234645454553
1104	15	0.4071937985406251
1104	16	0.48833123816572055
1104	17	0.6295490508458101
1104	18	0.4702212215477246
1104	19	0.5564177898532989
1104	20	0.5140203325234225
1104	21	0.41197718997297983
1104	22	0.4603523168732693
1104	23	0.4596039461162391
1104	24	0.42735216489605676
1104	25	0.5089794483787529
1104	26	0.566427279202081
1104	27	0.5914591247078533
1104	28	0.42611020893443197
1104	29	0.432675835441394
1104	30	0.3295525167190547
1104	31	0.4711610391485195
1104	32	0.5290852934369425
1104	33	0.5383801980009224
1104	34	0.5811826914699196
1104	35	0.5502050702452763
1104	36	0.5064894609836657
1104	37	0.5254698621799676
1104	38	0.5488483884194224
1104	39	0.548187680590857
1104	40	0.5257353215643619
1104	41	0.5737129169436841
1104	42	0.48533066609936526
1104	43	0.48447062317525535
1104	44	0.6045709445661629
1104	45	0.5890118209872739
1104	46	0.6053062532152609
1104	47	0.6888934672892333
1104	48	0.5991691283621066
1104	49	0.6965791647533663
1104	50	0.6340869618393867
1105	1	0.2982729036167129
1105	2	0.2389245221048668
1105	3	0.20723241204388287
1105	4	0.20799632625161024
1105	5	0.2564483450701829
1105	6	0.2983305577504538
1105	7	0.23474204937959797
1105	8	0.2689173924941528
1105	9	0.243707320314428
1105	10	0.19456364966922024
1105	11	0.3184589287463311
1105	12	0.22132038904626228
1105	13	0.35369292202670266
1105	14	0.4002938446798794
1105	15	0.2826963522931294
1105	16	0.15716523375334646
1105	17	0.3306670427798082
1105	18	0.23959888491592096
1105	19	0.23403257536062227
1105	20	0.2570810326015476
1105	21	0.29641787747893034
1105	22	0.3352413982841824
1105	23	0.3898669823939116
1105	24	0.32151720715816623
1105	25	0.27321615297807966
1105	26	0.43031030122761393
1105	27	0.4439328027629017
1105	28	0.44477001501141444
1105	29	0.3963928302155537
1105	30	0.4165256428827462
1105	31	0.2124487662221881
1105	32	0.40163441222717466
1105	33	0.42827802644141855
1105	34	0.4287831941731213
1105	35	0.4742758640874527
1105	36	0.38967545978898954
1105	37	0.4739020492868491
1105	38	0.4580300334066649
1105	39	0.5977049653436524
1105	40	0.48277976518108545
1105	41	0.5298912030654321
1105	42	0.5661672294647886
1105	43	0.5412358025633139
1105	44	0.6010413230757976
1105	45	0.6705650153889948
1105	46	0.597258572132283
1105	47	0.5333302706327387
1105	48	0.6339349880150849
1105	49	0.6136926097341444
1105	50	0.6222643316421497
1106	1	0.27253887590791237
1106	2	0.2673214995700377
1106	3	0.217585061154967
1106	4	0.19020605865775053
1106	5	0.2522590508641258
1106	6	0.2736789312367307
1106	7	0.2843847972150044
1106	8	0.2039886742630017
1106	9	0.25574186585367187
1106	10	0.18399000019678624
1106	11	0.2620445520228287
1106	12	0.2830193864793955
1106	13	0.3279271413924221
1106	14	0.4183714575711548
1106	15	0.37309644550477117
1106	16	0.2825381485153855
1106	17	0.46811439969804525
1106	18	-0.02864112551099396
1106	19	0.22219583186893743
1106	20	0.2568039603094405
1106	21	0.33448433052436854
1106	22	0.35901070438973903
1106	23	0.2139144964256232
1106	24	0.21044659451684566
1106	25	0.2786453055332174
1106	26	0.1704637470532191
1106	27	0.30337149247731077
1106	28	0.3052989881176913
1106	29	0.21297291537092633
1106	30	0.34310787341519244
1106	31	0.14047243014594102
1106	32	0.2592193661519957
1106	33	0.1439537023594113
1106	34	0.37902098062513545
1106	35	0.26879655685261383
1106	36	0.4022929143272975
1106	37	0.3555789563965064
1106	38	0.32028468462188897
1106	39	0.385505655741909
1106	40	0.45202294765054063
1106	41	0.31431756610739114
1106	42	0.21382657003422878
1106	43	0.17255826297274268
1106	44	0.2598305870157134
1106	45	0.4226816187060862
1106	46	0.42339173037873934
1106	47	0.3654995105876182
1106	48	0.3933479653311309
1106	49	0.35869764107857804
1106	50	0.47306940091825567
1107	1	-0.051819789342339106
1107	2	-0.1156728580796873
1107	3	-0.04604524940163657
1107	4	-0.1804311103896623
1107	5	-0.2025635247750941
1107	6	-0.17105421743750782
1107	7	-0.10055113868347831
1107	8	-0.08241465490157651
1107	9	-0.1482602761937386
1107	10	-0.20110573261428044
1107	11	-0.20405379164647997
1107	12	-0.3484441606781985
1107	13	-0.06587076885201526
1107	14	-0.2919950458915537
1107	15	-0.2726708106764377
1107	16	-0.24492825001950536
1107	17	-0.1791664791974199
1107	18	-0.13270556089779717
1107	19	-0.13762017580599917
1107	20	-0.09994852272956933
1107	21	-0.1338023705432363
1107	22	-0.38051747261999225
1107	23	-0.43323166250688416
1107	24	-0.3910782231334622
1107	25	-0.28579473057465776
1107	26	-0.020429095589989288
1107	27	-0.013979684612735355
1107	28	-0.10307507298850282
1107	29	-0.05213386008406928
1107	30	-0.23254049752392802
1107	31	-0.11456512633851901
1107	32	-0.1383045028940515
1107	33	-0.16318288266964487
1107	34	-0.10855187157439872
1107	35	-0.10006282120535559
1107	36	-0.26130998364553903
1107	37	-0.20466510199551635
1107	38	-0.1870016885051129
1107	39	-0.15077234358756897
1107	40	-0.215728849268487
1107	41	0.0349321219697174
1107	42	-0.10620066536350237
1107	43	-0.2061183564728708
1107	44	-0.0037307161685617984
1107	45	0.09379215270160302
1107	46	0.07269992506461165
1107	47	0.0026634693952303223
1107	48	-0.16073785318892675
1107	49	-0.054778243059622866
1107	50	-0.008094991043321897
1108	1	0.049907934600330606
1108	2	0.06394012633349533
1108	3	0.018989040096844434
1108	4	-0.0827629087985926
1108	5	-0.020007988885112127
1108	6	-0.11629913243247358
1108	7	-0.06766046825717353
1108	8	-0.059150907404067254
1108	9	-0.14839399247203033
1108	10	-0.17218919075668282
1108	11	-0.07880743707389826
1108	12	-0.20765245933833398
1108	13	-0.2155997517759971
1108	14	-0.28037817375330576
1108	15	-0.2449168215101949
1108	16	-0.04596874152996833
1108	17	-0.24482448950208635
1108	18	-0.11427536115568415
1108	19	-0.15093778341262265
1108	20	-0.2604615720391408
1108	21	-0.12872245018448325
1108	22	-0.2729082518800894
1108	23	-0.39446259657783145
1108	24	-0.21562486797278524
1108	25	-0.14135208440019653
1108	26	-0.2329942714571871
1108	27	-0.3287827113030559
1108	28	-0.463233738099035
1108	29	-0.5483233067684168
1108	30	-0.5196222068247422
1108	31	-0.4138861303674588
1108	32	-0.2608889370787395
1108	33	-0.46951411044039304
1108	34	-0.38660455867297117
1108	35	-0.32094639010004045
1108	36	-0.5432022175569955
1108	37	-0.49162355287151627
1108	38	-0.2868959704249008
1108	39	-0.33293704424990267
1108	40	-0.3718759250579211
1108	41	-0.2466263261079007
1108	42	-0.2082955681480172
1108	43	-0.23617242429659058
1108	44	-0.1822261527219169
1108	45	-0.28279572810310327
1108	46	-0.15904473771577443
1108	47	-0.22492120351701317
1108	48	-0.22705232819963328
1108	49	-0.27220675353384394
1108	50	-0.2093131073920631
1109	1	-0.2794019282782827
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2210	42	-0.3086280796793517
2210	43	-0.4021963116274634
2210	44	-0.3701162672806859
2210	45	-0.2534872219159823
2210	46	-0.1582646337638991
2210	47	-0.1952408480179102
2210	48	-0.17646415685570105
2210	49	-0.10898614797520167
2210	50	0.013738116835426695
2211	1	-0.2646835603694875
2211	2	-0.3523108090752771
2211	3	-0.3503236799352827
2211	4	-0.44959210374800307
2211	5	-0.6073880204916549
2211	6	-0.46592110790825103
2211	7	-0.4376997903791562
2211	8	-0.24927017296379717
2211	9	-0.3277368327204542
2211	10	-0.38502918321103863
2211	11	-0.3926428614854487
2211	12	-0.43657675054100764
2211	13	-0.48956078929839464
2211	14	-0.6920632173440566
2211	15	-0.4845812939590033
2211	16	-0.47768503030116705
2211	17	-0.3696114077391286
2211	18	-0.624300770718996
2211	19	-0.47244727021602273
2211	20	-0.5484449759139665
2211	21	-0.5933701826073445
2211	22	-0.6011139641598575
2211	23	-0.7992875097802852
2211	24	-0.5379095690840003
2211	25	-0.5834153547075687
2211	26	-0.5561226956878329
2211	27	-0.43555213473418775
2211	28	-0.3299547385987083
2211	29	-0.4394447368624341
2211	30	-0.6900918116052566
2211	31	-0.5498765516095361
2211	32	-0.3888844768001931
2211	33	-0.48992285315421213
2211	34	-0.42271418739822764
2211	35	-0.27423994315198996
2211	36	-0.3507099874456827
2211	37	-0.1970506122818847
2211	38	-0.3044229335357542
2211	39	-0.3734774830547565
2211	40	-0.45046542649660637
2211	41	-0.45744923416391714
2211	42	-0.4268463131732787
2211	43	-0.5680656744471051
2211	44	-0.5347252831870009
2211	45	-0.47425763731139625
2211	46	-0.550656484406538
2211	47	-0.5689652496280644
2211	48	-0.559983105046534
2211	49	-0.3814986348417406
2211	50	-0.33132697822165724
2212	1	-0.24077859671349344
2212	2	-0.3187687024175965
2212	3	-0.28027067950740303
2212	4	-0.19993573619376548
2212	5	-0.2369746454508217
2212	6	-0.26808828605777535
2212	7	-0.2766734762369367
2212	8	-0.11636834165796017
2212	9	-0.3134044871964221
2212	10	-0.30240916052229494
2212	11	-0.30727292175725296
2212	12	-0.21216472001838582
2212	13	-0.18950910509210672
2212	14	-1.0177605361106359
2212	15	-0.6202149281797418
2212	16	-0.5747519783905872
2212	17	-0.4261968245183425
2212	18	-0.40446989876291894
2212	19	-0.37676524877404205
2212	20	-0.2404056058824935
2212	21	-0.42097505802932744
2212	22	-0.48201777537166635
2212	23	-0.4763777296964804
2212	24	-0.43840150154596813
2212	25	-0.22194489080591495
2212	26	-0.36547968070863135
2212	27	-0.49472454861599857
2212	28	-0.4433669509056841
2212	29	-0.4099568713965738
2212	30	-0.34067495779503076
2212	31	-0.3195718570286843
2212	32	-0.23621606260283556
2212	33	-0.22339912729530198
2212	34	-0.19993392866422255
2212	35	-0.2728581248454489
2212	36	-0.3970036875032221
2212	37	-0.3064657125591097
2212	38	-0.4258515570252044
2212	39	-0.3716128000669414
2212	40	-0.30410940956322463
2212	41	-0.3572928095876975
2212	42	-0.25154885566751517
2212	43	-0.3321751840838232
2212	44	-0.19009270194354144
2212	45	-0.24797502867166088
2212	46	-0.2193632945456514
2212	47	-0.2154866517341958
2212	48	-0.27366782238473064
2212	49	-0.27061050253016816
2212	50	-0.12112789005882263
2213	1	-0.6705751232404609
2213	2	-0.695379503571516
2213	3	-0.7687827385505877
2213	4	-0.9551745008557191
2213	5	-0.6781492855831956
2213	6	-0.6638914168694683
2213	7	-0.9155995843949079
2213	8	-0.8379981063345525
2213	9	-0.8232029173974809
2213	10	-1.0045848403535942
2213	11	-0.9279859941407977
2213	12	-0.9431920450634124
2213	13	-0.8893491232502342
2213	14	-0.995593715685672
2213	15	-1.1577795432714808
2213	16	-0.9857387405338329
2213	17	-1.131143814516733
2213	18	-1.0320014192127687
2213	19	-1.1421593825291154
2213	20	-1.0972895842684665
2213	21	-1.1316037830070016
2213	22	-1.033702526942193
2213	23	-1.0766043401315386
2213	24	-1.1146451132550297
2213	25	-1.143030277910718
2213	26	-1.0494569684123647
2213	27	-1.158317879899215
2213	28	-1.1334890789204408
2213	29	-1.127733526494758
2213	30	-1.3031268168059995
2213	31	-1.1788354280284068
2213	32	-1.140688232351991
2213	33	-1.2479052703051536
2213	34	-1.2810176317496342
2213	35	-1.4454993994284706
2213	36	-1.5154341814334273
2213	37	-1.3081680365058617
2213	38	-1.234632985952267
2213	39	-1.42160071905829
2213	40	-1.1898139154133602
2213	41	-1.1851146403364297
2213	42	-1.1119198524605522
2213	43	-1.126483198551746
2213	44	-1.1646182477854765
2213	45	-1.1799205103744264
2213	46	-1.2177341888331625
2213	47	-1.238546734617234
2213	48	-1.364601208435193
2213	49	-1.4892932332774365
2213	50	-1.3211564451701854
2214	1	-0.691004841057385
2214	2	-0.9369813721008562
2214	3	-0.9252555965511426
2214	4	-0.9033671087427848
2214	5	-0.743761975767562
2214	6	-0.7596041231618429
2214	7	-0.7072918774435664
2214	8	-0.8428012225026045
2214	9	-1.0247544117816432
2214	10	-0.8976203219099261
2214	11	-1.0819712626320594
2214	12	-1.0323004666247186
2214	13	-0.966658426359075
2214	14	-1.0809204154469825
2214	15	-0.9199886838358466
2214	16	-0.8658530341975279
2214	17	-0.7110761608682665
2214	18	-0.86581982334814
2214	19	-0.9286129109158239
2214	20	-1.011333207692445
2214	21	-1.0757638966416962
2214	22	-1.0785486954319978
2214	23	-1.0574486195909003
2214	24	-1.2225266900829226
2214	25	-1.2813340310774066
2214	26	-1.2849744016739422
2214	27	-1.2528316376054391
2214	28	-1.1224770564863746
2214	29	-1.0255300237344258
2214	30	-1.1264969789111703
2214	31	-1.0944587348567367
2214	32	-1.2079355196164059
2214	33	-1.0787912841580436
2214	34	-1.2006880764771921
2214	35	-1.3650526949811947
2214	36	-1.16397201329508
2214	37	-1.0941731032076731
2214	38	-1.1677444680357638
2214	39	-1.2021180791016306
2214	40	-1.0869148394274788
2214	41	-1.07524152753642
2214	42	-1.0428384484276947
2214	43	-1.2137739774473175
2214	44	-0.8873023550355512
2214	45	-0.8646794027289815
2214	46	-0.9556000969589213
2214	47	-0.8293629714928663
2214	48	-0.9650328299262121
2214	49	-0.9783782250037731
2214	50	-1.0113993832714918
2215	1	-0.4222713855718929
2215	2	-0.46036327686529077
2215	3	-0.4409356172603047
2215	4	-0.3905892354721061
2215	5	-0.43689096856454057
2215	6	-0.4407552049438337
2215	7	-0.35075454409960116
2215	8	-0.30468375382846347
2215	9	-0.35926356300429774
2215	10	-0.2975299777485816
2215	11	-0.503771140420838
2215	12	-0.3052743238183382
2215	13	-0.37707612855766826
2215	14	-0.29368656376439617
2215	15	-0.4168374180577388
2215	16	-0.529876824039107
2215	17	-0.3428511977044053
2215	18	-0.3415740892966568
2215	19	-0.4499260037901536
2215	20	-0.48906343837278143
2215	21	-0.4715275115422557
2215	22	-0.39967404876804125
2215	23	-0.49131726201555637
2215	24	-0.46655822370976807
2215	25	-0.5637015841446313
2215	26	-0.5565505307323022
2215	27	-0.6447669223608443
2215	28	-0.46671311966645135
2215	29	-0.44726710509527123
2215	30	-0.4358524505511028
2215	31	-0.39261336897469334
2215	32	-0.5527630727249857
2215	33	-0.6366648930012033
2215	34	-0.4517556895868111
2215	35	-0.516427194714062
2215	36	-0.4685576513791432
2215	37	-0.5639079568273928
2215	38	-0.49431653470792725
2215	39	-0.5402953391306795
2215	40	-0.6126414764244004
2215	41	-0.6344324954239298
2215	42	-0.5057373884860112
2215	43	-0.6342691957632951
2215	44	-0.544227896561523
2215	45	-0.5603812770380685
2215	46	-0.7622211736404125
2215	47	-0.6560757671381552
2215	48	-0.6378338695781132
2215	49	-0.706866221398279
2215	50	-0.5849462938644407
2216	1	-0.46968663732862836
2216	2	-0.5003324317951545
2216	3	-0.5160937890843016
2216	4	-0.3812586825015529
2216	5	-0.44769481773860775
2216	6	-0.5654274659803349
2216	7	-0.4899768768993411
2216	8	-0.4864609385698415
2216	9	-0.4866525318544106
2216	10	-0.46901153025686426
2216	11	-0.8874387565289084
2216	12	-0.6926146430486639
2216	13	-0.6510610168956887
2216	14	-0.6738357021009023
2216	15	-0.7770429980278237
2216	16	-0.6903158010647843
2216	17	-0.7041199636391795
2216	18	-0.566329922709059
2216	19	-0.6948100622585187
2216	20	-0.6992737621854559
2216	21	-0.783212544617939
2216	22	-0.6939280240071994
2216	23	-0.841858362869651
2216	24	-1.0496596066912787
2216	25	-1.0105340901652795
2216	26	-0.906071725615412
2216	27	-0.9657151256556915
2216	28	-0.953094213737856
2216	29	-1.074651713647505
2216	30	-1.0315739486364137
2216	31	-1.2178254994848743
2216	32	-1.0856209855553587
2216	33	-1.0816072098939244
2216	34	-1.0947608634032235
2216	35	-1.1239501619775112
2216	36	-1.216976942467383
2216	37	-1.366121292475455
2216	38	-1.4327733122459279
2216	39	-1.3295076392079892
2216	40	-1.4311319251515329
2216	41	-1.3358002966427946
2216	42	-1.3592791817392609
2216	43	-1.4650314606150516
2216	44	-1.6085521412307564
2216	45	-1.6963169141966894
2216	46	-1.9864294263115987
2216	47	-1.74182631368938
2216	48	-1.9024431485342248
2216	49	-1.8834713661460363
2216	50	-2.057677313952091
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	145090.0
3	7124.0
4	13348.0
5	10414.0
6	8942.0
7	7749.0
8	6867.0
9	6234.0
10	6139.0
11	6096.0
12	6426.0
13	6639.0
14	7016.0
15	7557.0
16	8001.0
17	8801.0
18	9511.0
19	10873.0
20	13525.0
21	17199.0
22	21048.0
23	24257.0
24	26570.0
25	28751.0
26	32283.0
27	36911.0
28	42546.0
29	48935.0
30	57357.0
31	69427.0
32	84774.0
33	105548.0
34	135270.0
35	176909.0
36	235703.0
37	325677.0
38	513460.0
39	899383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01559064649382	17.06645186360341	12.796485223997061	25.1214722659057
2	16.19353879701019	18.982925196247812	39.13933767976917	25.684198326972822
3	20.167082141108033	22.972518845952155	27.844073465749737	29.016325547190075
4	13.424733900571177	15.396523187933361	35.965965729511645	35.212777181983824
5	15.030420872192026	36.49660644848796	33.58543652904446	14.88753615027555
6	34.23338508867037	34.78758274872469	16.80918273159884	14.169849431006106
7	29.998426009872066	30.67244006245593	20.357201319633322	18.97193260803868
8	26.733968383493227	35.00709284161335	19.09435147879294	19.16458729610048
9	26.436626369474876	16.202745246190656	18.558651303362296	38.80197708097217
10	15.097575263284964	28.80236936794296	32.847783722462374	23.252271646309698
11	36.292498633848815	22.453072392162195	22.135867197513985	19.118561776475
12	23.857601628460536	24.16699905587822	29.273599188036382	22.701800127624868
13	28.81127149306738	21.141415584996107	24.919240651212164	25.12807227072435
14	22.52424849497528	20.61739066928742	26.119371975627477	30.738988860109824
15	24.55407270764112	28.328530123288516	22.622694341131268	24.4947028279391
16	24.522734620521963	27.305469675472448	24.388471022515677	23.783324681489916
17	23.534398603285215	27.2437454294643	25.578535772715266	23.64332019453522
18	23.53846153846154	27.02935574758779	26.034345437660356	23.397837276290314
19	24.39549008718342	26.635271568231133	25.99641342798458	22.972824916600867
20	24.489049267825933	26.112240557490672	26.045239916135856	23.35347025854754
21	24.701498435501968	25.891064837949994	25.881020058385673	23.526416668162362
22	24.47838403278891	25.715682928027388	25.79569658167565	24.010236457508057
23	24.169260473159415	25.828960826727222	25.772294274443038	24.229484425670325
24	24.017672124839688	25.884354073509165	25.912126174764605	24.18584762688654
25	24.263723681699638	25.724782579396543	25.973141808805906	24.038351930097914
26	24.197606438419918	25.965256010827265	25.96475218267801	23.872385368074802
27	24.219942567822866	26.110512859265977	25.77756996397894	23.891974608932216
28	24.099419921335553	26.08577756964078	25.822523453992257	23.992279055031414
29	24.14167394599796	26.17262312251508	25.835023796643092	23.850679134843865
30	24.191958696402434	26.250431400407592	25.72132037494678	23.83628952824319
31	24.252059127592247	25.841657778723587	25.716680916757223	24.189602176926943
32	24.09484378748071	26.20399454265112	25.714293809107374	23.986867860760793
33	24.24190433316701	25.9507105990943	25.626049686058277	24.18133538168041
34	24.305105884804608	25.94815952494202	25.828349923364037	23.918384666889327
35	24.38497572769687	26.07991285896879	25.70449494090906	23.830616472425277
36	24.354854348759574	26.027611591655198	25.6651047759604	23.95242928362483
37	24.30587912323164	25.78025634115689	25.883077403544654	24.030787132066823
38	24.291634978116015	25.926858627931924	25.8099152845422	23.97159110940986
39	24.313395500939063	26.134854720840757	25.693183914494043	23.858565863726138
40	24.435785709855857	25.780978228532874	25.865473822263958	23.917762239347308
41	24.244913787622107	25.99802576204263	25.81309833858055	23.94396211175471
42	24.31642398567993	25.96765771326429	25.758597755989072	23.95732054506671
43	24.133274830866387	25.777397693601095	25.818536175109553	24.270791300422967
44	24.29855947545871	25.947854320944135	25.66955686433487	24.08402933926229
45	24.34169426490596	25.892787174065134	25.59279696182804	24.172721599200866
46	24.32710824206448	25.762065159682862	25.63570863664931	24.27511796160335
47	24.293838298734826	25.89051326686081	25.661423203528223	24.154225230876143
48	24.058241299320006	26.00673036585861	25.87590585040813	24.05912248441325
49	24.36512087675537	25.74672686429162	25.732564938722923	24.15558732023009
50	24.238716347311325	25.867924261092462	25.855052486313905	24.038306905282305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1585.0
1	1150.5
2	716.0
3	698.5
4	681.0
5	645.0
6	609.0
7	599.0
8	589.0
9	670.0
10	751.0
11	870.0
12	989.0
13	1473.0
14	1957.0
15	2646.5
16	3336.0
17	3802.0
18	4268.0
19	4813.0
20	5358.0
21	6505.0
22	7652.0
23	10016.0
24	12380.0
25	16451.5
26	20523.0
27	26430.0
28	32337.0
29	40194.0
30	48051.0
31	57581.0
32	67111.0
33	78579.5
34	90048.0
35	102426.0
36	114804.0
37	127084.5
38	139365.0
39	151219.5
40	163074.0
41	175464.0
42	187854.0
43	197611.5
44	207369.0
45	218485.0
46	229601.0
47	237475.0
48	245349.0
49	246452.0
50	247555.0
51	246646.0
52	245737.0
53	237931.0
54	230125.0
55	220255.0
56	210385.0
57	195825.5
58	181266.0
59	164454.0
60	147642.0
61	129686.5
62	111731.0
63	95501.0
64	79271.0
65	66033.0
66	52795.0
67	43431.0
68	34067.0
69	27814.0
70	21561.0
71	16933.0
72	12305.0
73	10095.0
74	7885.0
75	6027.0
76	4169.0
77	3339.5
78	2510.0
79	1915.0
80	1320.0
81	1027.0
82	734.0
83	566.5
84	399.0
85	319.5
86	240.0
87	175.0
88	110.0
89	98.0
90	86.0
91	56.5
92	27.0
93	24.5
94	22.0
95	17.0
96	12.0
97	11.0
98	10.0
99	24.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08614505594079966
2	0.09558388602927297
3	0.10304056179916686
4	0.10461370014724575
5	0.09668508287292818
6	0.059024150819919706
7	0.05411595917391359
8	0.060345587032305976
9	0.06166702324469223
10	0.04653343233617337
11	0.048830214324368536
12	0.0586151348494192
13	0.051536012283064224
14	0.05247989529191155
15	0.052385506991026815
16	0.05578348582287721
17	0.05546885815326143
18	0.05694760820045558
19	0.06506500207654262
20	0.0714519437697429
21	0.08120540152783196
22	0.08343925798210398
23	0.059244390188650756
24	0.07900300784052153
25	0.0855158006015681
26	0.08413143885525869
27	0.07676915138624951
28	0.09756604034785235
29	0.08501239633018287
30	0.08356510904995029
31	0.05575202305591563
32	0.05430473577568305
33	0.05710492203526347
34	0.05200795378748789
35	0.059779257226997576
36	0.05877244868422709
37	0.06191872538038485
38	0.05059212927421689
39	0.05619250179337772
40	0.0586151348494192
41	0.04524345889074869
42	0.040303804477780994
43	0.04046111831258888
44	0.037251916082507955
45	0.029040133905536187
46	0.026523112548609975
47	0.03168300633030872
48	0.02583093167545527
49	0.025988245510263155
50	0.027215293421764684
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3178360.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.30591541229872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.82664238497946	50.047134820317126
2	12.80512334713356	16.46890357609644
3	4.2795217669194665	8.255956942458432
4	1.8696770948969272	4.809251884510168
5	1.011215699479417	3.251357561715593
6	0.5517742120366227	2.1289407483548906
7	0.37101514654567036	1.6700928041313245
8	0.27074570709143025	1.3928440430771616
9	0.17151329791691647	0.9926387665136659
>10	0.8032088187425444	8.654040570864483
>50	0.031337444459107036	1.3454021642488698
>100	0.007831573001653054	0.7897910224226515
>500	3.443184477195943E-4	0.1502789689818943
>1k	4.918834955726667E-5	0.04336612630729135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.146276696157767E-5
2	0.0	0.0	0.0	0.0	3.146276696157767E-5
3	0.0	0.0	0.0	0.0	3.146276696157767E-5
4	0.0	0.0	0.0	0.0	3.146276696157767E-5
5	0.0	0.0	0.0	0.0	3.146276696157767E-5
6	0.0	0.0	0.0	0.0	6.292553392315533E-5
7	0.0	0.0	0.0	0.0	6.292553392315533E-5
8	0.0	0.0	0.0	0.0	6.292553392315533E-5
9	0.0	0.0	0.0	0.0	6.292553392315533E-5
10	0.0	0.0	0.0	0.0	6.292553392315533E-5
11	0.0	0.0	0.0	0.0	6.292553392315533E-5
12	0.0	0.0	0.0	0.0	9.4388300884733E-5
13	0.0	0.0	0.0	0.0	9.4388300884733E-5
14	0.0	0.0	0.0	0.0	1.2585106784631067E-4
15	0.0	0.0	0.0	3.146276696157767E-5	1.88776601769466E-4
16	0.0	0.0	0.0	3.146276696157767E-5	1.88776601769466E-4
17	0.0	0.0	0.0	3.146276696157767E-5	2.202393687310437E-4
18	0.0	0.0	0.0	3.146276696157767E-5	2.202393687310437E-4
19	0.0	0.0	0.0	3.146276696157767E-5	2.5170213569262133E-4
20	0.0	0.0	0.0	6.292553392315533E-5	2.8316490265419903E-4
21	0.0	0.0	0.0	6.292553392315533E-5	2.8316490265419903E-4
22	0.0	0.0	0.0	9.4388300884733E-5	3.1462766961577667E-4
23	0.0	0.0	0.0	1.2585106784631067E-4	4.7194150442366503E-4
24	0.0	0.0	0.0	2.202393687310437E-4	5.034042713852427E-4
25	0.0	0.0	0.0	2.202393687310437E-4	5.034042713852427E-4
26	0.0	0.0	0.0	6.292553392315533E-4	5.034042713852427E-4
27	0.0	0.0	0.0	8.494947079625971E-4	5.034042713852427E-4
28	0.0	0.0	0.0	0.0012270479115015292	5.034042713852427E-4
29	0.0	0.0	0.0	0.0020450798525025486	5.034042713852427E-4
30	0.0	0.0	0.0	0.003429441598811966	5.034042713852427E-4
31	0.0	0.0	0.0	0.007739840672548106	5.663298053083981E-4
32	0.0	0.0	0.0	0.014000931297902062	6.921808731547087E-4
33	0.0	0.0	0.0	0.021677846436527013	6.921808731547087E-4
34	0.0	0.0	0.0	0.03089643715626927	8.180319410010194E-4
35	3.146276696157767E-5	0.0	0.0	0.04234888433028354	8.180319410010194E-4
36	3.146276696157767E-5	3.146276696157767E-5	0.0	0.05729369863703294	8.180319410010194E-4
37	3.146276696157767E-5	3.146276696157767E-5	0.0	0.07686353968713425	8.180319410010194E-4
38	3.146276696157767E-5	3.146276696157767E-5	0.0	0.09841553505581495	8.494947079625971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2865	0.0	15.899083	1
CGTGCCG	255	0.0	15.527005	10
GTATCAA	4430	0.0	15.349036	1
GTACATG	7925	0.0	13.800139	1
TACATGG	7845	0.0	13.435543	2
TAGACTG	785	0.0	13.173252	5
GTGTAGC	1340	0.0	13.137465	1
GTCCTAT	535	0.0	12.750708	1
TATCAAC	5325	0.0	12.479582	2
TCAACGC	5375	0.0	12.404821	4
CAACGCA	5385	0.0	12.380031	5
ATCAACG	5410	0.0	12.365243	3
ACATGGG	8315	0.0	12.200147	3
TATACTG	765	0.0	12.079602	5
TACACAG	1955	0.0	12.0420885	5
GCACCGT	530	0.0	12.036629	6
TAGCCCT	1140	0.0	11.967769	4
GTGCCGA	350	1.6370905E-11	11.941007	11
GTATAAC	500	0.0	11.882836	1
AACGCAG	5600	0.0	11.823913	6
>>END_MODULE